RESUMO
The use of two monoclonal antibody types specific to different epitopes of diphtheria toxin systems have been developed to reveal diphtheria corynebacteria toxigenicity rapidly based on immunochromatographic and latex-agglutination detection of the diphtheria toxin. The methods have been tested on a sample of 36 clinical isolates. The possibility of significant detection of the toxigenic properties of the Corynebacterium strain, grown for 1 day, has been demonstrated. The developed methods allow for the detection of diphtheria toxin in concentrations of 3-4 ng/ml. The developed test systems are a perspective tool for diphtheria diagnostics because of significant time shortening as compared to traditional microbiological methods.
Assuntos
Corynebacterium diphtheriae/patogenicidade , Toxina Diftérica/análise , Anticorpos Monoclonais/imunologia , Cromatografia/métodos , Corynebacterium diphtheriae/crescimento & desenvolvimento , Corynebacterium diphtheriae/isolamento & purificação , Difteria/microbiologia , Toxina Diftérica/imunologia , Humanos , Imunoensaio/métodos , Testes de Fixação do Látex , Sensibilidade e EspecificidadeRESUMO
Latex diagnostic kit with high specificity and sensitivity of diphtheria toxin and toxoid detection has been developed on the basis of protective monoclonal antibodies to diphtheria toxin and polyacrolein microspheres. Diagnostic kit was stable during 1 year of shelf-life (time of the study).
