RESUMO
Introduction.Modern structure of hepatitides etiology and clinical course significantly changed. Prevalence of acute hepatitis tends to reduce, whilst number of patients with chronic hepatitis dramatically rises. Chronic hepatitis fraction is currently extended with mixed forms and lesions caused by toxic agents including drugs.Taking into account this issue, it is necessary to draw attention to the complimentary therapies supplemented with herbal medicines.Our research is focused on measuringpharmacotherapeutic efficiency of«BAIKALSKI - 7»dietary supplement in experimental liver lesions in white rats.Materials and Methods: Dietary supplement «BAIKALSKI - 7» consists of raw material of 7 medicinal plants: calendula flowers(Calendula officinalis L.), rose hips (Rosa majalisHerrm.), thornapple fruits (Grataegussanguinea L.), elecampane rhizomes(Inulahelenium L.), immortelle flowers (Helichrysum are-narium L.),licorice roots(Glicyrrhizaglabra L.)and peppermint leaves(Menthapiperita L.)in equal portions. The composition is based on pharmacological attributes and designed in the Institute of General and Experimental Biology of Siberian Division of the Russian Academy of Sciences(SD RAS). To determine features of «BAIKALSKI - 7» an experimental study in 90 rats of both genders with initial weight ranging from 160,0 to 200,0 was conducted. Chronic toxic hepatitis model was created by subcutaneous injection of CCl4 50% oil solution 0,2ml per 100 g of animal’s weight twice a week during 2 month. All rats were divided into 4 groups: I – intact rats, II –control group with chronicССl4 – hepatitis, III - comparison group with chronic ССl4 – hepatitis received standard treatment drug Carsil(Silibinin50 mg/kg), IV - chronic ССl4 – hepatitis treated with «BAIKALSKI - 7» injected into rats’ stomach. «BAIKALSKI - 7» infusion was used in dosage of dry extract 0,3 g per kgof animal’s weight once a day for 30 days after the 31st day of trials. II group animals received distilled water in equal volume.Functional condition of the liver was measured on 44th, 58th and 72ndday using approved biochemical methods. State of antioxidant defense was rated by means of serum antioxidant enzymes catalase, superoxide dismutases, and glutathione (GSH). Measuring of serum catalase activity is based on salts of ammonium molybdate ability to dye hydrogen peroxide. SOD activity was measured by inhibiting adrenaline autooxidationreaction(рН 10,2). Lipid peroxidation was tracked with malondialdehyde and conjugated dienes concentration in serum. Data is statistically approved forр< 0,05(Student t-test).Results: After injecting hepatotoxic CCl4 model showed increasing cytolytic markers (AST, ALT), cholestasis markers (ALP, ß-lipoprotein, cholesterol) and signs of dysproteinemia(thymol
