Assessment of Antimicrobial and Antioxidant Potential of Oscillatoria sancta and Oscillatoria proteus Isolated from Chilika Lake.

Filamentous cyanobacteria are a promising source of biologically active secondary metabolites with antioxidant, antimicrobial, antiviral and anticancer properties. Previously, cyanobacteria isolated from fresh and marine water were studied extensively, but those isolated from brackish water were less investigated. The purpose of this study was to examine the antimicrobial activities as well as the potential antioxidant capacity of two cyanobacterial strains (Oscillatoria proteus and Oscillatoria sancta) obtained from Chilika Lake. The pigment and antioxidant was assayed using a spectrophotometer; antimicrobial activity was studied by minimum inhibitory concentration (MIC); and the presence of phytoconstituents was detected using gas chromatography mass spectrometry (GC-MS). The solvents used for extraction were methanol, acetone and benzene. The experimental data indicates that the total phenolic and flavonoid content was highest in O. sancta (58.26 ± 0.72 µg/g, 38.45 ± 0.79 µg/g, respectively). Similarly, the methanol extract of O. sancta presented the maximum antioxidant potential in both DPPH (83.18 ± 0.57%) and ABTS (68.42 ± 1.40%) radicals. Besides, more reducing power was also recorded in methanol extract of O. sancta as compared to O. proteus. Further, higher enzymatic activity (superoxide dismutase and catalase) was observed in O. sancta. The antimicrobial potential against bacterial and fungal pathogens demonstrated better activity in O. sancta. In GC-MS analysis seven major chemical classes have been detected. Differential results was found in the two species of Oscillatoria; however, both have potential antimicrobial and antioxidant properties. The findings have pharmaceutical and nutraceutical importance.

Antioxidant and antimicrobial activities and GC/MS-based phytochemical analysis of two traditional Lichen species Trypethellium virens and Phaeographis dendritica.

BACKGROUND: Lichens are complex plants living in symbiotic relationship between fungi and algae. They are used for human and animal nutrition and are used in folk medicine in many countries over a considerable period of time. In the present study, various solvent extracts of Trypethelslium virens and Phaeographis dendritica were tested for their antioxidant and antimicrobial activity. RESULTS: The phytochemical analysis by GC/MS revealed phenolics (1.273%), terpene (0.963%), hydrocarbons (2.081%), benzofurans (2.081%), quinone (1.273%), alkanes (0.963%), and aliphatic aldehydes (0.963%) as the predominant compounds in Trypethellium virens SPTV02, whereas secondary alcohol (1.184%), alkaloids (1.184%), and fatty acids (4.466) were the major constituents in Phaeographis dendritica. The antioxidant property of methanolic extract of T. virens and P. dendritica revealed the presence of total phenolic and terpenoids. The methanolic extracts of both the lichens exhibited encouraging DPPH antiradical activity, with the IC50 of 62.4 ± 0.76 µg/ml for T. virens and 68.48 ± 0.45 µg/ml for P. dendritica. Similarly, ferric reducing power assay result exhibited higher reducing activity. Further, the lichen extracts (methanolic) indicated promising antimicrobial activities against pathogens showing MIC from 62.5 to 500 µg/ml. CONCLUSION: The study results concludes that both the lichens could be used as new natural source of antioxidants and antimicrobial agents which can be exploited for pharmaceutical applications.

Commercial Initiation of Feather Hydrolysate as Supreme Fertilizer: A Smart Bio-Cleaning Strategy of Poultry Waste.

Purpose: Economic development of India mainly depends on agricultural sectors. The Indian traditional agricultural system is mainly based on chemical fertilizer to get better yield. The main motto of this research work is to change the traditional faith of Indian farmers and rural Indian economy. Methods: Bioprocessing of feather prepared from an efficient newly isolated bacterial strain, identified as Bacillus wiedmanni SAB10 is used to produce a nitrogen rich liquid fertilizer. The cell-free hydrolysate was prepared from submerged fermentation of poultry litter (1.25%, w/v) as sole media with supplemented as chicken feather (1%, w/v) in 79.41 h with pH 10.6. Results: Fermented hydrolysate contains a significant quantity of total amino acid (503.02 mg/L) with diversity (Cystine, Phenylalanine, Tyrosine, lysine, Valine, Proline and Alanine), total oligopeptides (4.65 mg/ml) and thiol content (58.09 µg/ml) which influence growth and yield (1.02 fold) of moong beans (Vigna radiata) plant in pot trials and as well as successfully scale up in field trials by the farmers. This liquid fertilizer not only makes plant healthy and has drought tolerance (proline content- 0.023 mg/g) capacity but also increases the grain quality by spraying the fertilizer on foliage with a ratio of 2:1 (Water: Feather hydrolysate) for two times (before the 1st flash and 2nd flash of flowering). Conclusion: Fermented feather hydrolysate is used full as a foliage fertilizer for the cultivation of moong beans. Some commercial properties and its eco-friendly, cost-effectiveness will make it a smart liquid fertilizer in near future. Supplementary Information: The online version contains supplementary material available at 10.1007/s12649-022-01982-9.

Phlebotomy for rapid weaning and extubation in COPD patient with secondary polycythemia and respiratory failure.

The increased incidence of ventilator-associated complications in patients with chronic obstructive pulmonary disease (COPD) necessitates rapid weaning and extubation. The presence of secondary polycythemia in this subgroup increases the incidence of stroke and myocardial infarction due to hyperviscosity and tissue hypoxia. We present a 58-year-old male patient of COPD with secondary polycythemia (hematocrit 64%) who had possible hyperviscosity-related complications leading to cardiac arrest after a minor surgical procedure. The patient developed ventilator dependence after recovery. Phlebotomy was done to remove 10% of total blood volume. Symptomatic improvement was dramatic. Improvement in weaning indices like rapid shallow breathing index and PaO(2)/PAO(2) was observed facilitating rapid weaning and early extubation. Monitored, acute phlebotomy is safe and cost-effective. It decreases blood volume and viscosity, increases cardiac output and improves exercise tolerance in patients.