Bioelectrochemical approach for enhancing lignocellulose degradation and biofilm formation in Geobacillus strain WSUCF1.

Investigations on microbial electrocatalysis as a strategy for enhancing the rates of substrate utilization leading to enhanced yield of biomass and enhanced biofilm formation are reported. A thermophilic Geobacillus sp. strain WSUCF1 (60 °C), a potential lignocellulose degrading microorganism was used as the electrocatalyst. Glucose, cellulose, and corn stover were used as the feedstocks. The results of this investigation showed that applying the oxidation potential of -0.383 mV (vs PRE) increased the glucose utilization and COD removal by 25.5% and 29.7% respectively. The bioelectrocatalysis strategy also increased the biomass yield by 81.2, 42.1, and 49.5% in the case of systems fed with glucose, cellulose, and corn stover, respectively, when compared with the systems without applied oxidation potential. This is the first work reporting the effects of applied oxidation potential on increasing the rates of degradation of lignocellulosic biomass and enhanced biofilm formation.

Short term atmospheric pressure cold plasma treatment: A novel strategy for enhancing the substrate utilization in a thermophile, Geobacillus sp. strain WSUCF1.

The aim of this study was to investigate the effect of atmospheric pressure cold plasma on the microbial substrate utilization and biomass yield in a thermophilic strain. Geobacillus sp. strain WSUCF1, a thermophile capable of producing cellulolytic enzymes with higher activity was used for this investigation. Treatment with cold plasma for 4 min increased the rates of glucose utilization by 74% and biomass yield by 60% when compared with the control. WSUCF1 treated with plasma also displayed enhanced biofilm formation. This study for the first time, reports the use of cold plasma for enhancing the substrate utilization and biofilm formation in a thermophile.