RESUMO
A prolonged and compromised wound healing process poses a significant clinical challenge, necessitating innovative solutions. This research investigates the potential application of nanotechnology-based formulations, specifically nanofiber (NF) scaffolds, in addressing this issue. The study focuses on the development and characterization of multifunctional nanofibrous scaffolds (AZL-CS/PVA-NF) composed of azilsartan medoxomil (AZL) enriched chitosan/polyvinyl alcohol (CS/PVA) through electrospinning. The scaffolds underwent comprehensive characterization both in vitro and in vivo. The mean diameter and tensile strength of AZL-CS/PVA-NF were determined to be 240.42 ± 3.55 nm and 18.05 ± 1.18 MPa, respectively. A notable drug release rate of 93.86 ± 2.04%, was observed from AZL-CS/PVA-NF over 48 h at pH 7.4. Moreover, AZL-CS/PVA-NF exhibited potent antimicrobial efficacy for Staphylococcus aureus and Pseudomonas aeruginosa. The expression levels of Akt and CD31 were significantly elevated, while Stat3 showed a decrease, indicating a heightened tissue regeneration rate with AZL-CS/PVA-NF compared to other treatment groups. In vivo ELISA findings revealed reduced inflammatory markers (IL-6, IL-1ß, TNF-α) within treated skin tissue, implying a beneficial effect on injury repair. The comprehensive findings of the present endeavour underscore the superior wound healing activity of the developed AZL-CS/PVA-NF scaffolds in a Wistar rat full-thickness excision wound model. This indicates their potential as novel carriers for drugs and dressings in the field of wound care.
RESUMO
Ethanol distillation generates a huge volume of unwanted chemical liquid known as distillery wastewater. Distillery wastewater is acidic, dark brown having high biological oxygen demand, chemical oxygen demand, contains various salt contents, and heavy metals. Inadequate and indiscriminate disposal of distillery wastewater deteriorates the quality of the soil, water, and ultimately groundwater. Its direct exposure via food web shows toxic, carcinogenic, and mutagenic effects on aquatic-terrestrial organisms including humans. So, there is an urgent need for its proper management. For this purpose, a group of researchers applied distillery wastewater for fertigation while others focused on its physico-chemical, biological treatment approaches. But until now no cutting-edge technology has been proposed for its effective management. So, it becomes imperative to comprehend its toxicity, treatment methods, and implication for environmental sustainability. This paper reviews the last decade's research data on advanced physico-chemical, biological, and combined (physico-chemical and biological) methods to treat distillery wastewater and its reuse aspects. Finally, it revealed that the combined methods along with the production of value-added products are one of the best options for distillery wastewater management.
