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Mol Biol Rep ; 47(4): 2929-2939, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32236894

RESUMO

Drumstick (Moringa oleifera Lam.) is an important vegetable as well as forage crop of arid and semi-arid zones of the tropics. The leaves and pods of the plant are rich sources of minerals and vitamins. In the present work, genetic diversity study of 23 genotypes of M. oleifera collected from Kerala, Tamil Nadu and Karnataka states of India was carried out using seven cytochrome P450 (CytP450) markers. By using seven pairs of CytP450 gene-based markers, 88.25% of polymorphism was recorded among the 23 sampled genotypes. The Polymorphic Information Content (PI), Marker Index (MI) and Resolving Power obtained for seven primers were estimated 0.23, 2.96 and 9.83, respectively. The Unweighted Pair Group Method with Arithmetic mean (UPGMA) dendrogram based on this marker data indicate that genotypes from different geographical regions are placed in the same clusters. The dendrogram and Principal Coordinates Analysis (PCoA) plots derived from the binary data matrices were highly concordant. The investigation, in brief, proved that CytP450 based marker system is efficient in the elucidation of genetic diversity in M. oleifera accessions.


Assuntos
Sistema Enzimático do Citocromo P-450/genética , Moringa oleifera/genética , Moringa oleifera/metabolismo , Análise por Conglomerados , Sistema Enzimático do Citocromo P-450/metabolismo , Primers do DNA , Marcadores Genéticos/genética , Variação Genética/genética , Genótipo , Índia , Filogenia , Folhas de Planta/genética , Polimorfismo Genético/genética , Técnica de Amplificação ao Acaso de DNA Polimórfico/métodos
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