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J Biomed Opt ; 20(5): 051028, 2015 May.
Artigo em Inglês | MEDLINE | ID: mdl-25652791

RESUMO

The eardrum or tympanic membrane (TM) transforms acoustic energy at the ear canal into mechanical motions of the ossicles. The acousto-mechanical transformer behavior of the TM is determined by its shape, three-dimensional (3-D) motion, and mechanical properties. We have developed an optoelectronic holographic system to measure the shape and 3-D sound-induced displacements of the TM. The shape of the TM is measured with dual-wavelength holographic contouring using a tunable near IR laser source with a central wavelength of 780 nm. 3-D components of sound-induced displacements of the TM are measured with the method of multiple sensitivity vectors using stroboscopic holographic interferometry. To accurately obtain sensitivity vectors, a new technique is developed and used in which the sensitivity vectors are obtained from the images of a specular sphere that is being illuminated from different directions. Shape and 3-D acoustically induced displacement components of cadaveric human TMs at several excitation frequencies are measured at more than one million points on its surface. A numerical rotation matrix is used to rotate the original Euclidean coordinate of the measuring system in order to obtain in-plane and out-of-plane motion components. Results show that in-plane components of motion are much smaller (<20%) than the out-of-plane motions' components.


Assuntos
Holografia/métodos , Imageamento Tridimensional/métodos , Interferometria/métodos , Estroboscopia/instrumentação , Estroboscopia/métodos , Membrana Timpânica/fisiologia , Acústica , Algoritmos , Cadáver , Computadores , Audição , Humanos , Luz , Membranas Artificiais , Modelos Teóricos , Movimento (Física) , Reprodutibilidade dos Testes , Som , Vibração
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