In vivo antioxidant and hypoglycaemic potentials of Rivina humilis extract against streptozotocin induced diabetes and its complications in wistar rats.

Purpose: This current research study was designed to investigate beneficial effects of R. humilis (Rivina humilis) against streptozotocin-induced diabetic rats. Methods: The R. humilis ethanol extract was prepared using soxhlet and its phenol content was determined. The type-2 diabetes was induced in rats by giving fructose mixed drinking water and single dose of streptozotocin. Oral glucose tolerance test (OGTT) was performed after 72 h of streptozotocin to check ability of extract to utilize oral glucose load with 2 h. The extract was also tested for its potentials to reduce blood glucose (BGL) and diabetic complications by administering to diabetic rats for 21 days. Blood glucose was determined on day 1, 7, 14 and 21. At 21st day, blood samples were collected from experimental rats were euthanized to collect pancreas and liver. Liver and kidney function tests, HbAc1 and lipid profile was established from blood samples. Pancreas was subjected to histopathological examination and liver was used to determine antioxidant enzymes. In vitro study was done to investigate the effect of extract on glucose utilization by rat hemidiaphragm. Results: In OGTT, administration of extract could stimulate glucose utilization which was witnessed by significant BGL reduction at 90 and 120 min in therapeutic groups compare to diabetics. In chronic study, we observed significant reduction in BGL on 21st day and all tests performed to determine liver and kidney function, HbAc1, vitamin E were normal in extract treated groups. There was significant increase in liver antioxidant enzymes in therapeutic groups which revealed regeneration of ß-cells in therapeutic groups. Conclusion: The results of research demonstrated significant antidiabetic potentials in R. humilis. Supplementary Information: The online version contains supplementary material available at 10.1007/s40200-023-01258-6.

Gallic acid a flavonoid isolated from Euphorbia hirta antagonizes gamma radiation induced radiotoxicity in lymphocytes in vitro.

OBJECTIVES: The current study was executed to isolate and evaluate gallic acid from Euphorbia hirta for in vitro radioprotective potentials against gamma irradiation caused radiotoxicity in human lymphocytes. METHODS: The defatted E. hirta plant material was treated to methanol extraction using the soxhlet device. Bioflavonoids were isolated from the E. hirta methanol extract using column chromatography. In human cells exhibited to gamma radiation, separated flavonoid gallic acid was examined for in vitro radioprotective potentials using the micronucleus test, DNA fragmentation assay, superoxide free radical scavenging method, and apoptic assay. RESULTS: The frequency of micronuclei was considerably declined when cells were preprocessed with gallic acid (25 g/mL) before being exhibited to 2 Gy gamma radiation, as determined by the cytokinesis blocked micronucleus test. Similarly, pre-gamma radiation treatment of human cells with gallic acid led in markedly less DNA injury, as assessed by comet metrics like olive tail moment and percent tail DNA. Gallic acid (25 g/mL) given to lymphocytes prior to gamma irradiation considerably decreased the percentage of apoptotic bodies. Gallic acid also considerably lowered the reactive oxygen species concentrations elicited by gamma radiation. CONCLUSIONS: Our findings showed that gallic acid protects lymphocytes isolated from human blood from gamma radiation-induced DNA destruction and anti-apoptotic activity, which could be because of inhibition of free radicals formed by gamma radiation as well as the decline of gamma radiation-induced oxidative stress.

Attenuation of cisplatin induced myelosuppression by methanol extract of Cedrus deodara in Wistar rats.

OBJECTIVE: The present study was performed to evaluate the protective effects of methanol extract of Cedrus deodara against cisplatin induced. METHODS: Myelosuppression in albino Wistar rats. All experimental animals were administered with cisplatin on 1st, 3rd, 5th and 7th day to induce bone marrow toxicity and rats were treated with methanol extract of C. deodara for 21 days. Blood samples were collected from all the animals on day 1st, 7th, 14th and 21st after 1 h before the administration of the drugs and hematological parameters like RBC, WBC, platelets, hemoglobin, hematocrit, eosinophils, basophils, neutrophils, lymphocytes, bleeding time and clotting time were determined were determined. At 21st, all rats were sacrificed and bone marrow samples were collected. The part of bone marrow samples was used for the determination of antioxidant enzymes and remaining were subjected to histopathological examination. RESULTS: The animals of therapeutic groups administered with extract of C. deodara have exhibited significant rise in hematological parameters and shorten bleeding time and clotting time when compare to toxic control animals on the day 14 and day 21. The histopathological examination revealed the regeneration of bone marrow cells in the extract treated animals. There was significant reduction in lipid peroxidation and increase in antioxidant enzymes was found in extract treated animals. CONCLUSIONS: The methanol extract of C. deodara of have shown significant protective effects against cisplatin induced myelosuppression in albino Wistar rats.