Interpersonal memory failure in the workplace: The effect of memory and hierarchy on employee's affective commitment.

The current work examined whether being forgotten or remembered by a boss or a coworker affects employee's interpersonal closeness to that person and, in turn, affective organizational commitment (AOC). A first correlational study examined these possibilities in an employed student (1a) and general employed (1b) samples. Perceived memory by both bosses and coworkers was a significant predictor of closeness to the boss or coworker and, in turn, of AOC. The indirect effect of perceived memory on AOC was stronger for boss memory than coworker memory, but only when memory ratings were supported by specific examples of memory. Study 2 provided additional support for the direction of effects posited in Study 1 using vignettes depicting memory and forgetting in the workplace. Overall, these findings suggest that perceptions of boss and coworker memory have an effect on employee's AOC through interpersonal closeness, and that this indirect effect is stronger for boss memory.

Harnessing Split-Inteins as a Tool for the Selective Modification of Surface Receptors in Live Cells.

Biochemical studies of integral membrane proteins are often hampered by low purification yields and technical limitations such as aggregation causing in vitro manipulations to be challenging. The ability of controlling proteins in live cells bypasses these limitations while broadening the scope of accessible questions owing to the proteins being in their native environment. Here we take advantage of the intein biorthogonality to mammalian systems, site specificity, fast kinetics, and auto-processing nature as an attractive option for modifying surface proteins. Using EGFR as a model, we demonstrate that the split-intein pair AvaN /NpuC can be used to efficiently and specifically modify target membrane proteins with a synthetic adduct for downstream live cell application.

Chemical Labeling and Enrichment of Histone Glyoxal Adducts.

Because of their long half-lives and highly nucleophilic tails, histones are particularly susceptible to accumulating nonenzymatic covalent modifications, such as glycation. The resulting modifications can have profound effects on cellular physiology due to the regulatory role histones play in all DNA-templated processes; however, the complexity of Maillard chemistry on proteins makes tracking and enriching for glycated proteins a challenging task. Here, we characterize glyoxal (GO) modifications on histones using quantitative proteomics and an aniline-derived GO-reactive probe. In addition, we leverage this chemistry to demonstrate that the glycation regulatory proteins DJ-1 and GLO1 reduce levels of histone GO adducts. Finally, we employ a two-round pull-down method to enrich histone H3 GO glycation and map these adducts to specific chromatin regions.

Positive and negative emotions predict weight loss intentions and behaviors beyond theory of planned behavior constructs.

PURPOSE: The current study examined the predictive utility of emotional valence (i.e., positive and negative emotions) on weight loss intentions and behaviors, beyond theory of planned behavior constructs (i.e., attitude, subjective norm, and perceived control), among a community sample of people who were overweight (BMI > 25 kg/m2). METHODS: Participants were recruited for a longitudinal study via an online panel. They completed a baseline survey (N = 732) and a follow-up survey 6 months later (N = 526), both administered online. The surveys included measures of attitude, subjective norms, perceived control, positive and negative emotions regarding one's current weight, intentions to engage in weight loss behaviors (time 1), and having engaged in weight loss behaviors in the past 6 months (time 2). RESULTS: Emotion explained additional variance in weight loss intentions (range ΔR2 = 0.03-0.10, all ps < 0.01) and behaviors (range ΔR2 = 0.01-0.02, all ps < 0.05) beyond theory of planned behavior constructs. Negative emotions mainly predicted the intake of unhealthy food and seeking social support, whereas positive emotions predicted physical activity (intention and behavior). These results suggested that the differential relations might be based on whether the strategy is approach or avoidance oriented. CONCLUSIONS: Based on these findings, comprehensive models of weight loss behaviors should consider emotion, and the valence of such emotion, regarding current weight. LEVEL OF EVIDENCE: Level IV, multiple time series without intervention.

Utilizing intein trans-splicing for in vivo generation of site-specifically modified proteins.

Many cellular processes as well as their associated pathologies are regulated by protein post-translational modifications (PTMs). Understanding the precise roles of these adducts hinges on the development of methods to robustly and site-specifically manipulate proteins in their physiological environments. Recently, ultrafast intein protein trans-splicing (PTS) was harnessed to incorporate site-specific modifications on cellular chromatin in live cells. In this chapter, we present the protocols for the generation of synthetic modifications on native chromatin as well as highlight the capabilities of this methodology.

On being forgotten: Memory and forgetting serve as signals of interpersonal importance.

Our memories contain a wealth of social information-including details of past interactions, facts about others, and others' identities. Yet, human memory is imperfect, and we often find ourselves unable to recall such information in social interactions. Conversely, people routinely find themselves on the receiving end of others' memory failures; that is, people sometimes find themselves forgotten. Despite the apparent pervasiveness of such experiences, modern science possesses no explanatory framework for understanding the psychological impact of being forgotten in part or in whole. Here, we propose that evidence of memory in social interactions is a powerful signal of the subjective importance attached to an object of memory and that interpretation of such signals has important consequences for interpersonal relationships. We further proposed that attributional explanations for forgetting and that the closeness of the relationship between the people involved in forgetting might moderate the impact of being forgotten. We tested this framework in four studies examining the experience of being forgotten in daily life (Study 1), in experimentally controlled firsthand encounters (Study 2), and in third party perceptions of forgetting (Studies 3 and 4). Results converged to support our proposed framework as well as the moderating role of attribution. Surprisingly, we found no evidence supporting the moderating role of initial relationships closeness. These results advance a systematic model of an understudied but important phenomenon and suggest rich and varied avenues of additional exploration. (PsycINFO Database Record (c) 2019 APA, all rights reserved).

A Robust Method for the Purification and Characterization of Recombinant Human Histone H1 Variants.

Higher order compaction of the eukaryotic genome is key to the regulation of all DNA-templated processes, including transcription. This tightly controlled process involves the formation of mononucleosomes, the fundamental unit of chromatin, packaged into higher order architectures in an H1 linker histone-dependent process. While much work has been done to delineate the precise mechanism of this event in vitro and in vivo, major gaps still exist, primarily due to a lack of molecular tools. Specifically, there has never been a successful purification and biochemical characterization of all human H1 variants. Here we present a robust method to purify H1 and illustrate its utility in the purification of all somatic variants and one germline variant. In addition, we performed a first ever side-by-side biochemical comparison, which revealed a gradient of nucleosome binding affinities and compaction capabilities. These data provide new insight into H1 redundancy and lay the groundwork for the mechanistic investigation of disease-driving mutations.

Nitrimines as reagents for metal-free formal C(sp(2) )-C(sp(2) ) cross-coupling reactions.

Nitrimines are employed as powerful reagents for metal-free formal C(sp(2) )-C(sp(2) ) cross-coupling reactions. The new chemical process is tolerant of a wide array of nitrimine and heterocyclic coupling partners giving rise to the corresponding di- or trisubstituted alkenes, typically in high yield and with high stereoselectivity. This method is ideal for the metal-free construction of heterocycle-containing drug targets, such as phenprocoumon.

A motivational determinant of facial emotion recognition: regulatory focus affects recognition of emotions in faces.

Two studies examined an unexplored motivational determinant of facial emotion recognition: observer regulatory focus. It was predicted that a promotion focus would enhance facial emotion recognition relative to a prevention focus because the attentional strategies associated with promotion focus enhance performance on well-learned or innate tasks - such as facial emotion recognition. In Study 1, a promotion or a prevention focus was experimentally induced and better facial emotion recognition was observed in a promotion focus compared to a prevention focus. In Study 2, individual differences in chronic regulatory focus were assessed and attention allocation was measured using eye tracking during the facial emotion recognition task. Results indicated that the positive relation between a promotion focus and facial emotion recognition is mediated by shorter fixation duration on the face which reflects a pattern of attention allocation matched to the eager strategy in a promotion focus (i.e., striving to make hits). A prevention focus did not have an impact neither on perceptual processing nor on facial emotion recognition. Taken together, these findings demonstrate important mechanisms and consequences of observer motivational orientation for facial emotion recognition.

Perspective image comprehension depends on both visual and proprioceptive information.

Proprioceptive information can supplement visual information in the comprehension of ambiguous perspective images. The importance of proprioceptive information in unambiguous perspective image comprehension is untested, however. We explored the role of proprioception in perspective image comprehension using three experiments in which participants took or imagined taking an upward- or downward-oriented posture and then made judgments about images viewed from below or viewed from above. Participants were faster and more accurate in their judgments when their actual or simulated posture was consistent with the posture implied by the perspective of the image they were judging. These results support a role for proprioception in the comprehension of unambiguous perspective images as well as ambiguous perspective images.

Motivated shortcomings in explanation: the role of comparative self-evaluation and awareness of explanation recipient's knowledge.

Being aware of someone else's existing knowledge is a prerequisite to effectively adapting an explanation to that person's learning needs. However, such knowledge awareness introduces the potential for motivated self-evaluation based on relative knowledge, that is, for social comparison. Because favorable social comparisons are actively defended, we propose that knowledge awareness might undermine information sharing in explanation when social comparison motives are active. We tested this hypothesis in a series of experiments in which participants provided explanations to an ostensible learning partner with or without knowledge awareness. Both dispositionally and situationally motivated social comparison interacted with knowledge awareness to reduce information sharing in explanation. Intriguingly, knowledge awareness uniformly facilitated adaptation of the information that was shared to address partner knowledge deficit. These results illustrate a tension in the components of effective explanation. At the same time that knowledge awareness effectively coordinates explanation content, it can lead to knowledge hoarding by knowledgeable explainers who are motivated to rely on knowledge differences between the self and the explanation recipient for self-evaluation.

Implicit theories about groups and stereotyping: the role of group entitativity.

The consequences of holding an entity (i.e., the belief that a group's characteristics are fixed) or incremental (i.e., the belief that a group's characteristics are malleable) implicit theory about groups was examined for stereotyping and perceptions of group entitativity. Two studies showed that implicit theories about groups affect stereotyping by changing perceptions of group entitativity. Study 1 found that entity theorists were more likely to stereotype than incremental theorists and that perception of group entitativity significantly accounted for this relation. In Study 2, implicit theories of groups were manipulated via instruction set and entity theorists stereotyped more and perceived groups as more entitative than incremental theorists. Again, the effect of implicit theory was significantly, although partially, mediated by perceptions of group entitativity. The roles of implicit theories about groups and perceptions of group entitativity are discussed regarding stereotyping.