Assuntos
Anemia Hemolítica Autoimune/induzido quimicamente , Antibioticoprofilaxia/efeitos adversos , Cefotetan/efeitos adversos , Cefamicinas/efeitos adversos , Anemia Hemolítica Autoimune/sangue , Anemia Hemolítica Autoimune/terapia , Colecistectomia , Transfusão de Eritrócitos , Feminino , Hemoglobinas/análise , Herniorrafia , Humanos , Pessoa de Meia-Idade , Fatores de TempoRESUMO
The workshop on "Correlations of Aging and Space Effects on Biosystems" was held to explore the possibility that research in the space environment would be useful to the understanding of some phenomena of aging on Earth and that life sciences in space would benefit from this collaboration. The genesis and purpose of this workshop and the rationale for the concept are presented below. The unique characteristics of the space environment are described as background for discussion of the utility of this approach and the author's belief that carefully thought out selected projects would be of mutual benefit to the space and aging research communities.
Assuntos
Envelhecimento , Voo Espacial , HumanosAssuntos
Antivirais/uso terapêutico , Desoxiaçúcares/uso terapêutico , Desoxiglucose/uso terapêutico , Herpes Simples/tratamento farmacológico , Ceratite Dendrítica/tratamento farmacológico , Animais , Antivirais/farmacologia , Técnicas de Cultura , Desoxiglucose/farmacologia , Feminino , Doenças dos Genitais Femininos/tratamento farmacológico , Glicólise/efeitos dos fármacos , Humanos , Coelhos , Simplexvirus/efeitos dos fármacos , Simplexvirus/metabolismoAssuntos
Ceramidas/biossíntese , Desoxiaçúcares/farmacologia , Desoxiglucose/farmacologia , Gangliosídeo G(M3)/biossíntese , Gangliosídeos/biossíntese , Glicoesfingolipídeos/biossíntese , Simplexvirus , Linhagem Celular , Glucosiltransferases/metabolismo , Microssomos/enzimologia , Palmitatos/metabolismo , Serina/metabolismo , Uridina Difosfato GlucoseRESUMO
Human high density lipoprotein enriched in free cholesterol was obtained by exposing the lipoprotein to lipid dispersions having a free cholesterol/lecithin molar ratio greater than two. The metabolism of cholesterol was studied in tissue culture cells exposed to normal and cholesterol-enriched lipoproteins. Incubation of Fu5-AH rat hepatoma cells in medium containing cholesterol-enriched lipoprotein resulted in the accumulation of cellular cholesterol whereas normal high density lipoprotein produced no change in cellular content. The accumulated sterol was recovered primarily as esterified cholesterol and was derived almost entirely from lipoprotein free cholesterol. The esterification of incorporated free cholesterol and the cellular cholesterol content were directly related to the molar ratio of free cholesterol to phospholipid in the lipoprotein and to the concentration of lipoprotein in the culture medium. Isotopic experiments utilizing lipoprotein labeled with 125I or [4-14C]cholesteryl oleate demonstrated that a large fraction of the cholesterol incorporated from lipoprotein enriched in free cholesterol occurred by mechanisms that did not result in lipoprotein internalization and degradation. The response of other tissue culture cells to cholesterol/phospholipid dispersions is presented. The data indicate that the lipid composition of a lipoprotein can regulate free cholesterol uptake and esterification as well as cellular cholesterol content.
