Assuntos
Doenças dos Bovinos/diagnóstico , Pleuropneumonia Contagiosa/diagnóstico , Reação em Cadeia da Polimerase/veterinária , Animais , Búfalos/microbiologia , Bovinos , Doenças dos Bovinos/epidemiologia , DNA Bacteriano/análise , Surtos de Doenças/veterinária , Itália/epidemiologia , Mycoplasma mycoides/genética , Mycoplasma mycoides/isolamento & purificação , Pleuropneumonia Contagiosa/epidemiologia , Sensibilidade e EspecificidadeRESUMO
Slide agglutination tests using single absorbed and double absorbed antisera indicated that the Att 25 prototype Escherichia coli strain 25 KH9 produces the F(Y) adhesion; that this E coli also produces at least one other surface antigen not found on the F(Y) prototype E coli strain 11a; and that F(Y)+ E coli strain 28a produces at least one other surface antigen not produced by the prototype strains for the F(Y) and Att 25 antigens. These antigens were found on E coli isolated from outbreaks of calf diarrhoea in the United Kingdom.
Assuntos
Proteínas de Bactérias/análise , Doenças dos Bovinos/microbiologia , Diarreia/veterinária , Infecções por Escherichia coli/veterinária , Escherichia coli/imunologia , Adesinas de Escherichia coli , Testes de Aglutinação/veterinária , Animais , Bovinos , Diarreia/microbiologia , Epitopos , Infecções por Escherichia coli/microbiologiaRESUMO
Fifteen batches of E. coli vaccines for pigs, from three different manufacturers, were subjected to a quantitative form of the in vitro Limulus-amoebocyte-lysate (LAL) test for their free endotoxin content. A batch of vaccine associated with abortions in pregnant sows was found to contain a much higher level of free endotoxin than batches of vaccine not associated with abortion. The evidence of these assays suggests that they will be useful in the quality control of E. coli vaccines.