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1.
Neuron ; 110(14): 2258-2267.e11, 2022 07 20.
Artigo em Inglês | MEDLINE | ID: mdl-35397211

RESUMO

The amygdala and prelimbic cortex (PL) communicate during fear discrimination retrieval, but how they coordinate discrimination of a non-threatening stimulus is unknown. Here, we show that somatostatin (SOM) interneurons in the basolateral amygdala (BLA) become active specifically during learned non-threatening cues and desynchronize cell firing by blocking phase reset of theta oscillations during the safe cue. Furthermore, we show that SOM activation and desynchronization of the BLA is PL-dependent and promotes discrimination of non-threat. Thus, fear discrimination engages PL-dependent coordination of BLA SOM responses to non-threatening stimuli.


Assuntos
Tonsila do Cerebelo , Complexo Nuclear Basolateral da Amígdala , Tonsila do Cerebelo/fisiologia , Complexo Nuclear Basolateral da Amígdala/fisiologia , Medo/fisiologia , Interneurônios/metabolismo , Córtex Pré-Frontal/fisiologia , Somatostatina/metabolismo
2.
Neuron ; 89(4): 711-24, 2016 Feb 17.
Artigo em Inglês | MEDLINE | ID: mdl-26804990

RESUMO

Virally based transsynaptic tracing technologies are powerful experimental tools for neuronal circuit mapping. The glycoprotein-deletion variant of the SAD-B19 vaccine strain rabies virus (RABV) has been the reagent of choice in monosynaptic tracing, since it permits the mapping of synaptic inputs to genetically marked neurons. Since its introduction, new helper viruses and reagents that facilitate complementation have enhanced the efficiency of SAD-B19(ΔG) transsynaptic transfer, but there has been little focus on improvements to the core RABV strain. Here we generate a new deletion mutant strain, CVS-N2c(ΔG), and examine its neuronal toxicity and efficiency in directing retrograde transsynaptic transfer. We find that by comparison with SAD-B19(ΔG), the CVS-N2c(ΔG) strain exhibits a reduction in neuronal toxicity and a marked enhancement in transsynaptic neuronal transfer. We conclude that the CVS-N2c(ΔG) strain provides a more effective means of mapping neuronal circuitry and of monitoring and manipulating neuronal activity in vivo in the mammalian CNS.


Assuntos
Glicoproteínas/deficiência , Rede Nervosa/fisiologia , Neurônios/fisiologia , Vírus da Raiva/fisiologia , Potenciais de Ação/genética , Animais , Células Cultivadas , Estimulação Elétrica , Glicoproteínas/genética , Humanos , Proteínas Luminescentes/genética , Proteínas Luminescentes/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Vias Neurais/fisiologia , Neuroblastoma/patologia , Neurônios/virologia , Optogenética , Transporte Proteico , Proteínas do Envelope Viral
3.
Science ; 343(6173): 857-63, 2014 Feb 21.
Artigo em Inglês | MEDLINE | ID: mdl-24558155

RESUMO

Fear memories guide adaptive behavior in contexts associated with aversive events. The hippocampus forms a neural representation of the context that predicts aversive events. Representations of context incorporate multisensory features of the environment, but must somehow exclude sensory features of the aversive event itself. We investigated this selectivity using cell type-specific imaging and inactivation in hippocampal area CA1 of behaving mice. Aversive stimuli activated CA1 dendrite-targeting interneurons via cholinergic input, leading to inhibition of pyramidal cell distal dendrites receiving aversive sensory excitation from the entorhinal cortex. Inactivating dendrite-targeting interneurons during aversive stimuli increased CA1 pyramidal cell population responses and prevented fear learning. We propose subcortical activation of dendritic inhibition as a mechanism for exclusion of aversive stimuli from hippocampal contextual representations during fear learning.


Assuntos
Dendritos/fisiologia , Medo/fisiologia , Hipocampo/fisiologia , Aprendizagem/fisiologia , Inibição Neural , Tonsila do Cerebelo/metabolismo , Animais , Região CA1 Hipocampal/citologia , Região CA1 Hipocampal/fisiologia , Condicionamento Psicológico , Hipocampo/citologia , Interneurônios/metabolismo , Interneurônios/fisiologia , Camundongos , Receptores de Glicina/metabolismo , Receptores Nicotínicos/metabolismo , Somatostatina/metabolismo
4.
Nat Neurosci ; 16(9): 1182-4, 2013 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-23912949

RESUMO

Hippocampal interneurons receive GABAergic input from the medial septum. Using two-photon Ca(2+) imaging of axonal boutons in hippocampal CA1 of behaving mice, we found that populations of septo-hippocampal GABAergic boutons were activated during locomotion and salient sensory events; sensory responses scaled with stimulus intensity and were abolished by anesthesia. We found similar activity patterns among boutons with common putative postsynaptic targets, with low-dimensional bouton population dynamics being driven primarily by presynaptic spiking.


Assuntos
Hipocampo/fisiologia , Vias Neurais/fisiologia , Septo do Cérebro/citologia , Transdução de Sinais/fisiologia , Vigília/fisiologia , Ácido gama-Aminobutírico/metabolismo , Potenciais de Ação/fisiologia , Anestésicos Locais/farmacologia , Animais , Calmodulina/genética , Calmodulina/metabolismo , Channelrhodopsins , Colina O-Acetiltransferase/metabolismo , Condicionamento Psicológico , Proteínas de Ligação a DNA/genética , Proteínas de Ligação a DNA/metabolismo , Glutamato Descarboxilase/genética , Glutamato Descarboxilase/metabolismo , Hipocampo/efeitos dos fármacos , Técnicas In Vitro , Camundongos , Camundongos Transgênicos , Atividade Motora/efeitos dos fármacos , Atividade Motora/fisiologia , Vias Neurais/efeitos dos fármacos , Parvalbuminas/genética , Parvalbuminas/metabolismo , Lectinas de Plantas/metabolismo , Proteínas/genética , RNA não Traduzido , Septo do Cérebro/efeitos dos fármacos , Septo do Cérebro/fisiologia , Tetrodotoxina/farmacologia , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo
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