Valve gape behaviour of mussels (Mytilus edulis) exposed to dispersed crude oil as an environmental monitoring endpoint.

Environmental monitoring requires cost-effective and efficient methods for detecting potential effects of pollution, and valve gape behaviour has been used with this purpose for a range of contaminants in freshwater and marine bivalves. The current study investigated the use of a new method for measuring valve behaviour responses in mussels (Mytilus edulis) exposed to dispersed crude oil (DCO). Results confirmed that valve gape is a sensitive parameter; at the high DCO concentration (0.25mgL-1) the mean valve gape was reduced from 49 to 31%, and mussels increased shell movement (measured as distance travelled) or spent more time closed to avoid contact with the oil. At the low DCO concentration (0.015mgL-1) the distance travelled parameter was the most sensitive endpoint. Results also demonstrated that valve gape behaviour is a valid endpoint when monitoring mussels for exposure to DCO.

Effect of dispersed crude oil on the feeding activity, retention efficiency, and filtration rate of differently sized blue mussels (Mytilus edulis).

The use of physiological response endpoints in environmental monitoring represents an opportunity to provide an integrated picture of health status and ecological fitness of individuals, and may provide an indication of potential longer term effects on aquatic organisms in the environment. The feeding behavior response sensitivity of blue mussels (Mytilus edulis) of differing size to dispersed crude oil (DCO) was investigated in a lab exposure experiment. The ability of mussels to recover following a single exposure was also investigated, as well as the response to consecutive exposures, in order to assess the utility of employing the same individuals in chronic environmental monitoring. Feeding physiology was assessed by measuring retention efficiency and filtration rate of individual mussels in a live-algae feeding assay. In addition, the percentage of mussels actively filtering during testing was calculated. The feeding physiology parameters were sensitive and able to discriminate exposed mussels from controls. Further, data indicated that larger mussels appear more suitable in environmental monitoring, as these animals showed both sensitivity and an ability to adapt and recover from exposure while remaining sensitive to subsequent treatments. Smaller mussels were also sensitive to the measured endpoints, even if these animals suffered higher rates of mortality during the exposure. Finally, when exposed to the high concentration of DCO, mussels displayed a tendency to close the valves and terminate filtration.