Progesterone receptors in extratesticular ducts of the Amazonian stingray Potamotrygon wallacei: A potential role in sperm maturation and aggregate formation.

In cururu stingray (Potamotrygon wallacei Carvalho, Rosa and Araújo 2016) males, plasma progesterone (P4) levels appear to be associated with spermiation events. However, the specific contribution of P4 in sperm maturation via extratesticular ducts in this stingray species is unknown. With the aim of filling this knowledge gap, this study examined the morphology and the presence of progesterone receptors (PR) in the ducts, and analyzed the relationship of progesterone (P4) with sperm maturation and formation of aggregates. Morphological analysis showed that a columnar pseudostratified epithelium with stereocilia lined all the attached ducts. In active males, the secretory cells of the epididymis and the Leydig glands presented PR; however, these receptors were not found in the distal region of the epididymis (essential for nurturing and capacitation events) of regressing males. In the seminal vesicles of active males, the spermatozoa are parallelly aligned and embedded in a matrix to form the spermatozeugmata. The matrixes are formed by proteins secreted by the ducts and Sertoli cell cytoplasts. These structures presented PR, which suggests that P4 engages in sperm metabolism during storage. Our findings allude to the potential role of P4 in regulating the development and function of the attached ducts in different reproductive phases. Furthermore, P4 seems to be an essential component for regulating sperm progress, protein secretion, aggregate formation, and maintenance of sperm during storage in this freshwater stingray.

Characterization of education, technical practices and attitudes of Portuguese pharmacy technicians towards manipulation of cytotoxic drugs.

Pharmacy professionals that manipulate cytotoxic drugs need to undergo educational programs, adopt the most convenient practices, and use appropriate equipment to avoid, as far as possible, occupational exposure to cytotoxic drugs. The main goal of this work is to characterize the education, technical practices, and attitudes towards cytotoxic drugs, of Portuguese pharmacy technicians. A questionnaire comprising eleven questions deemed pertinent was elaborated and subsequently validated by a pilot test. The anonymous, web-based survey was conducted between December 2022 and January 2023, by graduated pharmacy technicians that had manipulated cytotoxic drugs between 2017 and 2022. A total of 77 pharmacy technicians responded to the survey. Although sixty-six pharmacy technicians (86%) had been trained before beginning to manipulate cytotoxic drugs, the promotion of regular post-admission training by the institutions is sparse - only assumed by 53% of the pharmacy technicians (n = 41). All participants reported using gloves and gown during manipulation and the use of double gloves was common (99%; n = 76). Compliances with the recommended limit time for uninterrupted manipulation activity (82%; n = 63) and systematic double-checking (86%; n = 66) were high, but the regular use of sterile gauze around syringe connection sites 58% (n = 45), was less frequent. None of the surveyed pharmacy technicians used closed-system transfer devices (CSTD) and 41 (53%) of those who used spikes did not thoughtfully use these devices. The implementation of regular training programs in manipulating cytotoxic drugs should be fostered, to promote the more judicious use of engineering controls and transversal adoption of the safest technical practices.

Facial Pain: RCT between Conventional Treatment and Fascial Manipulation® for Temporomandibular Disorders.

Background: To investigate the effectiveness of a specific manual therapy, Fascial Manipulation® (FM), in comparison with conventional treatments in temporomandibular disorders (TMD) patients using a two-arm randomized controlled trial. Methods: The study consisted of 28 patients that were divided in two groups (Group 1: Fascial Manipulation® vs. Group 2: conventional TMD treatment). The Verbal Rating Scale (VRS), RDC/TMD, electromyography (EMG) and Pression/Pain Evaluation on Masseter and Temporalis Muscle were assessed with different times. Results: In both groups, the improvement in pain was evident on the VRS scale (p < 0.0001) and pain-free opening (p < 0.001). In Group 1, the recovery of the function was faster; maximum unassisted opening T0 vs. T1 (p = 0.001). Conclusions: FM® can be used as an effective method for facial pain, being a rapid, safe and cost-effective approach to reduce pain, gain function and mouth opening that can be used prior to occlusion stabilization appliances.

Indicações da fototerapia em recém-nascidos com icterícia / Indications for phototherapy in newborns with jaundice

A fototerapia é um método eficaz, não invasivo de alto impacto, sem restrições enquanto a sua utilização independente da idade gestacional, comorbidades ou nível de pigmentação cutânea, tratamento utilizado para redução de bilirrubina usado devido a sua alta eficiência e ausência de efeitos adversos. Analisar as evidências científicas publicadas sobre as indicações da fototerapia em recém­nascidos com icterícia. Trata de uma revisão bibliográfica do método revisão integrativa de literatura, realizado no período compreendido entre os meses de janeiro a abril de 2021. A busca efetuou-se, através da BVS, utilizando as bases de dados LILACS, MEDLINE, e por meio do SCIELO. Aderindo-se através dos descritores/palavras chaves: "Fototerapia", "Icterícia Neonatal", "Terapêutica", "Diagnóstico", "Cuidados de Enfermagem", combinados entre si com o operador booleano "AND". Os profissionais da equipe de enfermagem são responsáveis pelo recebimento e preparação do recém-nascido para a terapêutica, bem como, preparam os aparelhos que serão usados para a fototerapia, como os focos de luz, as incubadoras, entre outros. Evidenciou-se que a fototerapia é utilizada como forma de tratamento mais eficaz no controle da hiperbilirrubinemia. Sendo indicada nas doenças hemolíticas ou não, incompatibilidade fator Rh e grupo ABO. Embora seja frequente o uso da técnica em neonatos, deve-se enquanto profissionais se atentar aos sinais e sintomas, como também os fatores de risco no manejo da técnica, intervir elaborando uma rotina para o controle e manutenção dos equipamentos, observando criteriosamente o tipo de aparelhos utilizados e se atentar a distância ideal das lâmpadas sob o RN, avaliando sempre possíveis complicações durante o tratamento.

Phototherapy is an effective, non-invasive, high-impact method, with no restrictions as long as its use regardless of gestational age, comorbidities or skin pigmentation level, treatment used to reduce bilirubin used due to its high efficiency and absence of adverse effects. Analyze the published scientific evidence on the indications for phototherapy in newborns with jaundice. This is a bibliographic review of the integrative literature review method, carried out between January and April 2021. The search was carried out, through the VHL, using the LILACS, MEDLINE databases, and through SCIELO . Adhering to the descriptors / keywords: "Phototherapy", "Neonatal Jaundice", "Therapeutics", "Diagnosis", "Nursing Care", combined with the Boolean operator "AND". The professionals of the nursing team are responsible for receiving and preparing the newborn for therapy, as well as preparing the devices that will be used for phototherapy, such as spotlights, incubators, among others. It became evident that phototherapy is used as the most effective treatment for controlling hyperbilirubinemia. Being indicated in hemolytic diseases or not, incompatibility Rh factor and ABO group. Although the technique is frequently used in neonates, professionals should pay attention to the signs and symptoms, as well as the risk factors in the management of the technique, to intervene by elaborating a routine for the control and maintenance of equipment, carefully observing the type of equipment. devices used and pay attention to the ideal distance of the lamps under the NB, always evaluating possible complications during treatment.

Innovation in justice: co-production, competence and user satisfaction in judicial mediation / Innovación en el Poder Judicial: coproducción, competencias y satisfacción del usuario en la mediación judicial / Inovação no Judiciário: coprodução, competências e satisfação do usuário na mediação judicial

Abstract This study evaluated the effects of co-production and the mediator's competence on the results of Judicial Mediation in the Court of Justice of the Brazilian Federal District and Territories. Data was collected using satisfaction surveys applied to the parties using the court's judicial mediation services. The sample refers to the period from January 2013 to July 2015, totaling 312 participants and 107 discursive answers. The evaluation adopted techniques of descriptive statistics, exploratory factor analysis, and logistic regression as well as content analysis. The results showed that the only statistically significant variable was the mediator's competence, which increases the likelihood of positive perception about the outcomes of judicial mediation both for citizens and the justice system. Both parties using the services did not perceive that co-production affects the outcomes. This result was due to the complexity of the cases mediated, which makes it hard to observe the role of cooperation in achieving the outcomes. For the citizen, the mediator is primarily responsible for directing the judicial mediation procedure as well as the outcomes achieved through the procedure. We provide recommendations and a research agenda aimed at strengthening research in the area.

Resumen Este estudio evaluó los efectos de la coproducción y las competencias de los mediadores en los resultados de la mediación judicial en el Tribunal de Justicia del Distrito Federal y Territorios. Los datos se recopilaron a través de encuestas de satisfacción de los usuarios realizadas por el Tribunal a los usuarios de los servicios de mediación judicial. La muestra se refiere al período comprendido entre enero de 2013 y julio de 2015, con un total de 312 participantes y 107 respuestas discursivas. La evaluación se realizó mediante técnicas de estadística descriptiva, análisis factorial exploratorio y regresión logística, así como análisis de contenido. Se constató que la única variable estadísticamente significativa era la competencia del mediador, lo que aumenta la probabilidad de una percepción positiva de los resultados de la mediación judicial para el usuario del servicio y el Poder Judicial. También se observó que la coproducción no se percibe como un factor que afecta los resultados del servicio para los ciudadanos o el Poder Judicial. Este resultado se debió a la complejidad de los casos mediados, lo que hizo que la percepción de cooperación para llegar a un resultado sea difícil de observar. Consiguientemente, para el ciudadano, el mediador es el principal responsable de dirigir el procedimiento de mediación judicial, así como de los resultados logrados a través del procedimiento. Finalmente, se enumeran recomendaciones y una agenda de investigación para fortalecer la investigación en el área.

Resumo O presente estudo teve por objetivo testar os efeitos da coprodução e das competências do mediador nos resultados da mediação Judicial no Tribunal de Justiça do Distrito Federal e Territórios - TJDFT, com base na Pesquisa de Satisfação do Usuário - PSU, aplicada pelo TJDFT às partes usuárias dos serviços de mediação judicial. Os dados analisados correspondem à amostra tabulada no período de janeiro de 2013 a julho de 2015, totalizando 312 participantes e 107 respostas discursivas. Os objetivos propostos foram alcançados a partir de técnicas de estatística descritiva, análise fatorial exploratória e regressão logística, além de análise de conteúdo. Por meio dos testes realizados foi constatado que a única variável que apresentou significância estatística foi a de competências do mediador, comprovando-se que as competências do mediador aumentam a probabilidade de percepção positiva dos resultados da Mediação Judicial para o usuário do serviço e para o Judiciário. Tem-se ainda, que a coprodução não pode ser percebida como um fator que impacta nos resultados do serviço para o cidadão e para o judiciário. Esse resultado se deu devido à complexidade dos casos mediados, o que faz com que a percepção de cooperação para se chegar a um resultado seja de difícil observação. Por conseguinte, observa-se que para o cidadão, o mediador é o principal responsável pelo direcionamento do procedimento de mediação judicial, bem como pelos resultados alcançados com o procedimento. Ao final do estudo foram elencadas recomendações e agenda de pesquisa, com vistas ao fortalecimento de pesquisas na área.

Morphological description of ovary and uterus of the nurse shark (Ginglymostoma cirratum) caught off at the Fortaleza coast, Northeast Brazil / Descrição morfológica de ovário e útero de Tubarões-lixa (Ginglymostoma cirratum) capturados na costa de Fortaleza, Nordeste do Brasil

The nurse shark, Ginglymostoma cirratum (Bonnaterre, 1778) is one of the most studied species of elasmobranchs. However, the knowledge of their reproductive biology is still relatively rare, particularly in the western South Atlantic. This study aimed to describe the morphology of the uterus and the ovary of G. cirratum, based on specimens caught off at the Fortaleza/CE coast, northeast Brazil. Samples were collected from September 2012 to June 2013, from regular landings of artisanal fishing, which commercialize this species freely. A total of ten females were collected. The methodologies followed for analyzing the ovaries and uterus of those females included both macroscopic and histological analysis. G. cirratum has internal type ovary morphology, with invaginations of connective tissue, which defines compartments and separate oocyte groups in ovigerous lots. The epithelium lining the ovary changes from simple columnar ciliated in the area without ovigerous lots, which turns into a simple cubic epithelium in the coating portion of the epigonal organ where ovarian tissue is absent. The uterine mucosa has secretory cells denoted by Alcian Blue staining, indicating the production of mucopolysaccharides, even in immature individuals. This lecithotrophic shark has a uterine vascularized mucosa that is one characteristic of viviparous elasmobranch species.(AU)

O tubarão-lixa, Ginglymostoma cirratum (Bonnaterre, 1778) é uma das espécies mais estudadas de elasmobrânquios. O conhecimento de sua biologia reprodutiva, no entanto, ainda é relativamente raro, particularmente no Oeste do Atlântico Sul. Este estudo objetivou a descrição morfológica do útero e ovário de G. cirratum, baseado em espécimes capturados na costa de Fortaleza/CE, no Nordeste do Brasil. Um total de dez fêmeas foi coletado de setembro de 2012 a junho de 2013, nos desembarques regulares da pesca artesanal, que comercializam essa espécie livremente. A metodologia foi de acordo com as analises macroscópica e histológica dos ovários e útero. G. cirratum tem o tipo de morfologia de ovário interno, com invaginações de tecido conjuntivo, que define compartimentos e separa grupos de oócitos em lotes ovígeros. O epitélio que reveste o ovário varia de epitélio simples colunar ciliado na área que não apresenta lotes ovígeros para epitélio simples cúbico na porção de revestimento do órgão epigonal, onde o tecido ovariano é ausente. A mucosa uterina possui células secretoras, evidenciadas pela coloração em Alcian Blue, indicando a produção de mucopolissacarídeos, mesmo em indivíduos imaturos. Este tubarão lecitotrófico apresenta a mucosa uterina vascularizada, sendo esta uma característica de espécies de elasmobrânquios vivíparos.(AU)

Bacterial Profile and Radiographic Analysis Around Osseointegrated Implants With Morse Taper and External Hexagon Connections: Split-Mouth Model.

The aim of this clinical study was to evaluate bacterial colonization, marginal bone loss, and optical alveolar density in implants with Morse taper (MT) and external hexagon (EH) connections. Thirty-five implants were installed in 7 patients (mean age: 65.8 ± 6.7 years). Implants were divided into 2 groups, according to platform design: G1 - MT, installed 2mm infra-osseous and G2 - EH, positioned according to Branemark protocol. Patients were evaluated at baseline (T0), 21 days (T1), 3 months (T2), 6 months (T3), and 12 months (T4) after installations. Bone loss and alveolar density were evaluated by standardized periapical radiographs and bacterial profile with checkerboard DNA-DNA hybridization. Statistical analyses were performed using SPSS 23.0. To present the results, boxplots and a line graph of mean were used. P-values ≤ .05 were statistically significant. After 3 months, alveolar bone loss was significantly higher in the G2 (T2-T0: P = .006; T3-T0: P = .003; and T4-T0: P = .005). No significant differences between G1 and G2 groups were observed for optical alveolar density. Microbiological analysis showed similar profiles between studied groups; however, there were significantly higher counts of Tannerella forsythia (P = .048), Campylobacter showae (P = .038), and Actinomyces naeslundii (P = .027) in G1 after 12 months. Based on the results of this study, it can be concluded that there was less peri-implant bone loss in MT compared to EH connections, but microbiological profile did not seem to influence bone changes.

Sexual selection and the evolution of sperm morphology in sharks.

Post-copulatory sexual selection, and sperm competition in particular, is a powerful selective force shaping the evolution of sperm morphology. Although mounting evidence suggests that post-copulatory sexual selection influences the evolution of sperm morphology among species, recent evidence also suggests that sperm competition influences variation in sperm morphology at the intraspecific level. However, contradictory empirical results and limited taxonomic scope have led to difficulty in assessing the generality of sperm morphological responses to variation in the strength of sperm competition. Here, we use phylogenetically controlled analyses to explore the effects of sperm competition on sperm morphology and variance in sharks, a basal vertebrate group characterized by wide variation in rates of multiple mating by females, and consequently sperm competition risk. Our analyses reveal that shark species experiencing greater levels of sperm competition produce sperm with longer flagella and that sperm flagellum length is less variable in species under higher sperm competition risk. In contrast, neither the length of the sperm head and midpiece nor variation in sperm head and midpiece length was associated with sperm competition risk. Our findings demonstrate that selection influences both the inter- and intraspecific variation in sperm morphology and suggest that the flagellum is an important target of sexual selection in sharks. These findings provide important insight into patterns of selection on the ejaculate in a basal vertebrate lineage.

Os efeitos da coprodução nos resultados da conciliação judicial: a percepção da sociedade sobre um serviço inovador / Los efectos de la coproducción en los resultados de la conciliación judicial: la percepción de la sociedad de un servicio innovador / The effects of coproduction on judicial conciliation results: society's perception of an innovative service

Resumo Este estudo examinou os efeitos da coprodução e das competências do prestador de serviços nos resultados da conciliação judicial no Tribunal de Justiça do Distrito Federal e Territórios (TJDFT), a partir da percepção de satisfação das partes litigantes e dos advogados usuários do serviço. A conciliação judicial é uma inovação do Poder Judiciário que visa a aumentar a eficiência e o valor gerado pelos serviços de Justiça e trata-se de um processo coproduzido, no qual os usuários participam da prestação dos serviços públicos. Para alcançar o objetivo proposto, utilizaram-se dados secundários provenientes da pesquisa de satisfação do usuário elaborada e aplicada pela equipe técnica do TJDFT e a amostra final, após o tratamento dos dados, consistiu em 1.735 partes litigantes e 764 advogados. Para análise dos dados, aplicaram-se as técnicas de análise fatorial exploratória e de regressão logística e os resultados sugerem que a coprodução é a variável com maior poder preditivo dos resultados do serviço, tanto para o cidadão como para a imagem do Poder Judiciário, na perspectiva das partes. Por fim, os resultados do serviço para o cidadão, se positivos, aumentam a probabilidade da imagem do Poder Judiciário também ser positiva, na percepção dos dois grupos investigados.

Resumen Este estudio examinó los efectos de la coproducción y las competencias del prestador de servicios en los resultados de la conciliación judicial en el Tribunal de Justicia del Distrito Federal y Territorios (TJDFT), a partir de la percepción de satisfacción de las partes litigantes y de los abogados usuarios del servicio. La conciliación judicial es una innovación cuyo propósito es aumentar la eficiencia y el valor generado por los servicios de justicia y se trata de un proceso coproducido, en que los usuarios tienen un papel esencial en la prestación de los servicios públicos. Para alcanzar el objetivo propuesto, se utilizaron datos secundarios provenientes de la encuesta de satisfacción del usuario, elaborada y aplicada por el equipo técnico del TJDFT y la muestra final, después del tratamiento de los datos, consistió en 1.735 partes litigantes y 764 abogados. Para el análisis de los datos, se aplicaron las técnicas de análisis factorial exploratorio y de regresión logística, y los resultados sugieren que la coproducción es la variable con mayor poder predictivo de los resultados del servicio, tanto para el ciudadano como para la imagen del Poder Judicial, desde la perspectiva de las partes. Por último, los resultados del servicio para el ciudadano, si fueran positivos, aumentan la probabilidad de que la imagen del Poder Judicial también sea positiva, según la percepción de los dos grupos investigados.

Abstract This study examined the effects of coproduction and the competencies of the service provider on the results of the Judicial Conciliation at the State Courthouse of Distrito Federal, based on the satisfaction of the litigants and lawyers who used the service. The Judicial Conciliation is an innovation to increase efficiency and results for the general public and is a coproduced process, in which the users play an essential role in the provision of public services. The study used secondary data obtained from a customer satisfaction survey designed by the TJDFT. The final research sample consisted of 1,735 litigants and 764 lawyers. For the data analysis, exploratory factor analysis and logistic regression were used, and the results of the regressions suggest that coproduction is the variable that best explains the results of the service for the customers as well as for the reputation of the Judiciary, from the perception of the litigants. Finally, the perception that the service was beneficial to citizens increases the likelihood of the customer to develop a positive image of the Judiciary, according to the results obtained both in the litigants' and lawyers' samples.

A case report of a sub-clinical necrotising lower limb infection secondary to pelvic anastomotic leak and chronic corticosteroid use.

Anabolic steroids have attracted attention from the media with regard to misuse, but overuse of medically prescribed steroids also has a propensity to cause harm. Gluteal necrotising soft tissue infections are an uncommon presentation for plastic surgeons, and therefore, abdominal sources may be overlooked. We report a rare case of a 57-year-old male who presented with a necrotising lower limb infection on a background of long-term corticosteroid overuse and recurrent pelvic anastomotic leaks.

Bilateral Low-Dose Computed Tomography Assessment for Post-Operative Rotational Malalignment After Intramedullary Nailing for Tibial Shaft Fractures: Reliability of a Practical Imaging Technique.

INTRODUCTION: The purpose of this study is to evaluate the intra- and inter-observer reliability of low-dose protocolled bilateral postoperative Computed Tomography (CT)-assessment of rotational malalignment after intramedullary nailing (IMN) of tibial shaft fractures. MATERIALS AND METHODS: 156 patients were prospectively included with tibial shaft fractures that were treated with IMN in a Level-I Trauma Centre. All patients underwent post-operative bilateral low-dose CT-assessment (effective dose of 0.03784 - 0.05768 mGy) as per hospital protocol. Four observers performed the validated reproducible measurements of tibial torsion in degrees, based on standardized techniques. The Intra-Class Coefficient (ICC) was calculated to evaluate intra- and inter-observer reliability. The intra- and inter-observer reliability was categorized according to Landis and Koch. RESULTS: Intra-observer reliability for quantification of rotational malalignment on post-operative CT after IMN of tibial shaft fractures was excellent with 0.95 (95% CI = 0.92-0.97). The overall inter-observer reliability was 0.90 (95% CI = 0.87-0.92), also excellent according Landis and Koch. CONCLUSION: Firstly, bilateral post-operative low-dose -similar radiation exposure as plain chest radiographs- CT assessment of tibial rotational alignment is a reliable diagnostic imaging modality to assess rotational malalignment in patients following IMN of tibial shaft fractures and it allows for early revision surgery. Secondly, it may contribute to our understanding of the incidence-, predictors- and clinical relevance of post-operative tibial rotational malalignment in patients treated with IMN for a tibial shaft fracture, and facilitates future studies on this topic.

Mapeamento de competências de suporte e de apoio pedagógico e administrativo de profissionais que atuam na modalidade a distância / Mapping the management and pedagogical support competences of professionals working with distance learning

RESUMO: O presente estudo teve como objetivo diagnosticar, empiricamente, as competências profissionais (técnicas e comportamentais), de apoio e suporte administrativo, pedagógico e secretarial, de cursos a distância ofertados no âmbito da Universidade Aberta do Brasil. Por meio de duas etapas empíricas foi realizada pesquisa com desenho multimétodo sequencial, que envolveu fase qualitativa, composta por análise documental e realização de nove entrevistas semiestruturadas, e fase quantitativa, com aplicação de questionário totalmente estruturado. O tratamento dos dados foi feito por análise de conteúdo e análise estatística. Os resultados indicaram baixas lacunas de competência, o que sugere que as atividades de captação, treinamento e desenvolvimento de competências provavelmente têm sido realizadas de forma condizente com as necessidades das atribuições das equipes secretariais e de apoio. Recomenda-se, para pesquisas futuras, que se utilize avaliação por múltiplas fontes e a validação psicométrica do instrumento de competência, para identificar suas evidências de validade interna e externa.

ABSTRACT: The aim of the present study was to empirically diagnose the professional competencies (technical and behavioral) concerning the management, pedagogical and secretarial support in distance learning courses provided by Brazil Open University. A sequential mixed model design was applied through two empirical stages, which encompassed one qualitative stage composed of documental analysis and of the conduction of nine semi-structured interviews; and the was based on the application of a totally structured questionnaire. Data treatment was conducted through content and statistical analysis. Results showed low competence gaps, fact that suggests that activities focused on recruitment, training and on competence development have been possibly performed in compliance with the attributes needed by the secretarial and support teams. It is recommended to apply data evaluations based on multiple sources and on the psychometric validation of the competence instrument in further studies in order to identify its internal and external validity evidences.

Comparison of the Morphology and Histomorphometry of Spermatogenic Cyst of Three Sharks Species With Diametric Testes.

Characterization of the reproductive anatomy of elasmobranchs (sharks, skates, rays, and sawfish) offers unique insights into the evolution of reproductive traits in animals due to their phylogenetic position at the base of the vertebrate tree of life. Yet, despite advances in our understanding of male elasmobranch reproductive physiology and testes histology, very little is known about how testes histomorphometrics varies with male maturation. In this study, we characterize and contrast testes morphology and histomorphology of males at different maturation stages in three shark species with diametric testes development: Prionaceglauca, Rhizoprionodon lalandii, and Mustelus canis. All stages of spermatogenesis were observed in P. glauca and R. lalandii, while for M. canis, only males at early stages of maturation were examined and therefore all the spermatogenesis cells lineage were not present. The number of Sertoli cells increased with cell development by six times in R. lalandii and roughly four times in P. glauca, and were statistically different among stages of spermatogenesis cysts in both species. Statistical differences in cyst diameter and Sertoli cell numbers were observed between P. glauca and R. lalandii. The increase of spermatocyte II cell diameter described for R. Lalandii in this study was not usual to elasmobranch species as compared, for example, to P. glauca. This information proves the importance of studying the testicular development and the process of spermatogenesis is necessary for understanding the reproductive biology of the species, including life cycles and history, variation of reproductive morphology. Anat Rec, 299:759-768, 2016. © 2016 Wiley Periodicals, Inc.

Osseointegrated implants placed at supracrestal level may harbour higher counts of A. gerencseriae and S. constellatus - a randomized, controlled pilot study.

PURPOSE: This study aimed at evaluating the bacterial colonization in dental implants inserted in the crestal or supracrestal position and correlated it to radiographic bone measurements. METHODS: Thirty-five implants with regular platform in nine patients (mean age 62.4±11.2 years) were inserted either at the bone crest level (control group) or at a suprecrestal level (test group). Radiographic examination was performed at baseline (implant installation) and after 6 months. Clinical and microbiological data were collected after 6 months. Digital radiography was used to assess bone remodeling (marginal bone loss and optical alveolar density). Bacterial profile was analyzed by checkerboard DNA-DNA hybridization, including a panel of 40 bacterial species. RESULTS: After 6 months, there were significantly higher counts of Actinomyces gerencseriae (p=0.009) and Streptococcus constellatus (p=0.05) in the test group. No significant differences between test and control groups were observed for marginal bone loss (p=0.725) and optical alveolar density (p=0.975). Probing depth was similar in both groups. CONCLUSION: Significantly higher counts of A. gerencseriae and S. constellatus were found in implants placed at the supracrestal level compared to the ones placed at the bone level. No relation was found between the installation level of dental implants and peri-implant bone remodeling.

Planning and treatment in oral rehabilitation with implant-supported prostheses using cephalometric analysis / Planejamento e tratamento em reabilitação oral com próteses sobre implantes usando análise cefalométrica

There are growing prosthetic and esthetic demands for Oral Rehabilitations on osseointegratable implants, requiring precise prosthetic-surgical planning. In edentulous patients planning may be done using cephalometric analysis to determine the position of the teeth in the dental prosthesis, and consequently, those of the implants. In this clinical case, the planning and treatment of an oral implant-supported rehabilitation is described, using cephalometry to optimize prosthetic success and patient comfort. The patient presented complete mandibular and partial maxillary edentulism, with unsatisfactory esthetics and function of the anterior teeth, with accentuated vestibular inclination. In order to determine the correct position of maxillary teeth it was necessary to use a Steiner cephalometric tracing to position the maxillary central incisor in the diagnostic wax-up. Therefore, the maxillary anterior teeth were extracted, osseointegratable implants were placed (Neodent(r), Curitiba, Brazil), and an immediate temporary fixed denture was inserted. After 30 days, surgery was performed for the placement of 4 osseointegratable implants (Neodent(r), Curitiba, Brazil) in the inter-mentonian region, on which a complete, temporary, implant-supported denture was placed. After the period of osseointegration, the definitive implant-supported dental prosthesis were fabricated.

Reabilitações orais sobre implantes osteointegráveis enfrentam crescentes exigências protéticas e estéticas, necessitando de um planejamento protético-cirúrgico preciso. O planejamento em pacientes edêntulos pode ser realizado usando análise cefalométrica para determinar o posicionamento dos dentes na prótese e conseqüentemente dos implantes. Esse caso clínico descreve o planejamento e tratamento de uma reabilitação oral sobre implantes usando análise cefalométrica para otimizar o sucesso protético assim como o conforto do paciente. Paciente apresentando edentulismo total mandibular e parcial maxilar com elementos anteriores insatisfatórios estética e funcionalmente, com inclinação vestibular acentuada. Para a determinação do correto posicionamento dos elementos superiores foi necessário a utilização de traçado cefalométrico de Steiner para o posicionamento do incisivo central superior no enceramento diagnóstico. Dessa forma, foi realizado exodontia dos elementos anteriores superiores, instalação de implantes osteointegraveis (Neodent(r), Curitiba, Brasil) e instalação de prótese fixa provisória imediata. Após 30 dias, foi realizada cirurgia para instalação de 4 implantes osteointegráveis (Neodent(r), Curitiba, Brasil) na região intermentoniana, sobre os quais foi instalada uma prótese total implanto-suportada inferior provisória. Após período de osteointegração, as próteses sobre implantes definitivas foram confeccionadas.

Remodelação alveolar e perfil bacteriano em implantes instalados em diferentes níveis relacionados à crista óssea / Bone remodeling and bacterial profile in dental implants inserted in different levels in relation to the crestal bone

A substituição clínica de dentes naturais perdidos por implantes osteointegrados tem representado uma das primeiras opções terapêuticas para a reabilitação de pacientes total ou parcialmente edêntulos. Apesar dos excelentes índices de sucesso demonstrados pelas restaurações implanto-suportadas, alguns fatores permanecem não esclarecidos, principalmente no que diz respeito à remodelação óssea ao redor dos implantes osteointegrados. Desta forma, o objetivo deste estudo foi avaliar a relação do nível de instalação dos implantes dentários com os parâmetros clínicos, com a remodelação óssea peri-implantar e com a colonização bacteriana, em implantes de plataforma regular, submetidos à carga imediata. Implantes de plataforma regular foram instalados em dois diferentes níveis em relação à crista óssea – ao nível ósseo e supra ósseo (1 mm). No total, trinta e cinco implantes em 9 pacientes (idade média de 62,4 ± 11,2 anos) foram avaliados radiograficamente no momento da instalação dos implantes (T1) e 6 meses após (T2), momento no qual também foram feitas análises clínicas e coleta de amostras para o teste microbiológico. Nos exames radiográficos foram analisadas a perda óssea, a partir de mensurações lineares da distância entre um ponto fixo do componente protético e o ponto mais coronário do contato osso-implante, e a densidade óptica alveolar obtida a partir de regiões ósseas de interesse (ROIs). As análises clínicas consistiram na avaliação da profundidade de sondagem e na mensuração do volume do fluido gengival peri-implantar. O perfil bacteriano dos sítios avaliados foi caracterizado por meio do método de análise de checkerboard DNA-DNA hybridization. Os testes estatísticos realizados mostraram não haver relação entre o nível de instalação dos implantes em relação à crista óssea e a remodelação óssea alveolar, tanto com relação ...

The replacement of missing teeth with osteointegrated dental implants represents one of the first therapeutic options for the rehabilitation of totally or partially edentulous patients. In spite of clinical success rates for implant-supported restorations, some factors remain nuclear, especially those related to alveolar bone remodeling around osteointegrated implants. Thus, the aim of this study was to evaluate the relation between the installation level of dental implants with bone remodeling and bacterial colonization, in regular platform implants, under an immediate loading protocol. Dental implants with regular platform were inserted in different levels related to the bone crest – on the level of bone crest and above the bone crest. In total, thirty-five implants in 9 patients (mean age 62,4 ± 11,2 years) were radiographically evaluated in the moment of implant installation (T1) and 6 months after (T2), when clinical analysis and sample collecting for microbiologic test were performed. In radiographic exams were analyzed the marginal bone loss, from linear measurements of the distance between a fixed point of prosthetic components and the most coronal point of bone-implant contact, and optical alveolar density, from bone regions of interest (ROIs). Clinical analysis consisted of probing depth evaluation and peri-implant crevicular fluid volume mensuration. The bacterial profile of selected sites was characterized by checkerboard DNA-DNA hybridization method. Statistic tests showed no relation between implant insertion levels and alveolar bone remodeling, with relation to marginal bone loss (p = 0,725) and optical alveolar density (p = 0,975). It was not also possible to establish a correlation among alveolar ...

Perfil do paciente idoso referenciado ao consultório odontológico do Instituto Jenny de Andrade Faria HC/UFMG / Profile of elderly patient referenced to the doctor's office dental Instituteof Jenny de Andrade Faria HC/UFMG

Introdução: Com o aumento da expectativa de vida, medidas de saúde públicas devem ser planejadas e executadas para garantir atendimento à população idosa. Neste contexto, pode-se citar a saúde bucal como importante fator para garantir bem- estar e qualidade de vida. Objetivo: Este estudo foi realizado com o objetivo de identificar o perfil do paciente geriátrico encaminhado ao consultório odontológico do Instituto Jenny de Andrade Faria do HC/UFMG. Material e Método: Trata-se de um estudo transversal e descritivo, realizado por meio da coleta de dados dos prontuários de 48 pacientes. Resultado: Os principais resultados mostraram que esta população é composta, em sua maioria, por mulheres; estes indivíduos apresentam comprometimento sistêmico, grande parte está sob o uso de polifarmácia e a taxa de pacientes edêntulos é alta. Conclusão: Há necessidade da inserção do cirurgião-dentista na equipe multidisciplinar, que esteja apto a cuidar de pacientes complexos, bem como da instalação de equipamentos odontológicos para que o tratamento seja efetivamente realizado em consultório próprio da instituição.

Introduction: With increasing life expectancy, public health measures should be planned and implemented to ensure care for the elderly population. In this context, we can cite the oral health as an important factor in ensuring well being and quality of life. Objective: This study aimed to identify the profile of geriatric patients referring to the dental office of the Institute of Jenny de Andrade Faria HC / UFMG. Material and Method: This was a cross-sectional and descriptive study by collecting data from medical records of 48 patients. Result: The results showed that this population is composed mostly of women, who have systemic involvement, most is under the use of polypharmacy and the rate of edentulous patients is high. Conclusion: There is need to insert the dental surgeon in the multidisciplinary team that is able to take care of complex patients, as well as the installation of equipment for dental treatment to be carried out effectively in the office of the institution itself.

Morphological analysis and description of the ovaries of female silky sharks, Carcharhinus falciformis (Müller & Henle, 1839)

This work aims to study the female reproductive tract of silky sharks, Carcharhinus falciformis, captured in the South and Equatorial Atlantic Ocean. Samples were collected between January 2008 and March 2010 through oceanic commercial vessels that targeted tuna and swordfish, with a total of 17 females collected. The methodologies followed for analyzing the ovaries of those females included both macroscopic and histological analysis. Macroscopically, it was possible to determine that the ovaries on these sharks is suspended by mesenteries in the anterior section of the body cavity, heavily irrigated by blood vessels, and contains a wide range of oocytes. Ovaries were found in three distinct maturational stages: Stage I (Immature), Stage II (Maturing) and Stage III (Mature). Immature ovaries were small, with widths ranging from 1.0 to 3.1 cm, and had a gelatinous or granulose internal structure; maturing ovaries were slightly larger, ranging in width between 5.2 and 6.0 cm; mature ovaries ranged in width between 6.5 and 7.8 cm, and had a more rounded shape and the presence of large and well developed oocytes. Under microscopic examination, it was observed that the ovaries were covered with simple epithelial tissue during the early development stages and a simple cubic epithelium in the final stages of maturation. During the initial maturation stages the epigonal organ was not differentiated from the ovary. In mature specimens, the ovary showed a simple cubic epithelium and just below this epithelium there was a layer of dense connective tissue and muscle with the presence of vitellogenic oocytes and fat cells. A thin yolk membrane enclosing the oocytes was also evident. Finally, it was possible to distinguish a zona pellucida, separating the oocytes from the follicle wall and a basal lamina between the granular layers and the teak layer.

Este trabalho tem como objetivo estudar o trato reprodutivo feminino de tubarões lombo preto, Carcharhinus falciformis, capturados no Atlântico Sul e Equatorial. As amostras foram coletadas entre janeiro de 2008 e março de 2010, através das embarcações da frota atuneira, que tem como pesca alvo o atum e espadarte, com um total de 17 fêmeas coletadas. A metodologia seguida para analisar os ovários das fêmeas incluiu a análise macroscópica e histológica. Macroscopicamente, foi possível determinar que o ovário destes tubarões, é suspenso por mesentério na seção anterior da cavidade do corpo, muito irrigada por vasos sanguíneos, contendo uma vasta gama de ovócitos. Os ovários foram encontrados em três diferentes estágios de maturação: estádio I (imaturo), estágio II (maturação) e fase III (Maturo). Ovários imaturos foram pequenos, com largura variando de 1,0 a 3,1 centímetros, e tinham uma estrutura gelatinosa ou granulosa interna; ovários em maturação foram ligeiramente maiores, variando de largura entre 5,2 e 6,0 cm; ovários maduros variaram de largura entre 6,5 e 7,8 cm, possuíam uma forma mais arredondada e a presença de ovócitos grandes e bem desenvolvidos. Ao exame microscópico, foi possível observar que os ovários foram cobertos com tecido epitelial simples durante os estágios iniciais de desenvolvimento, e um epitélio cúbico simples na fase final de maturação. Durante os estágios iniciais de maturação, o órgão epigonal não foi diferenciado do ovário. Em espécimes maduros, o ovário mostrou um epitélio cúbico simples, e só abaixo deste epitélio havia uma camada de tecido conjuntivo denso e músculo com a presença de ovócitos vitelogênicos e células de gordura. Também foi evidenciada uma membrana fina juntando os ovócitos. Finalmente, foi possível distinguir uma zona pelúcida, separando os ovócitos da parede do folículo e uma lâmina basal entre as camadas granular e da camada teca.

O corpo idealizado de consumo: paradoxos da hipermodernidade / The idealized body of consumption: hypermodernity paradoxes

Em tempos hipermodernos, nos quais reina o paradoxo do "elogio da moderação e da cultura do excesso" (Lipovetsky, 2004), os corpos, cada vez mais, ganham visibilidade e são tratados enquanto mercadoria a ser vendida pela indústria cultural e pela indústria da saúde. A publicidade, não raro, dá a impressão de que um corpo perfeito, veiculado como sinônimo de saúde, bem-estar e felicidade, está ao alcance de todos, desde que seguidas as devidas prescrições. Assim, o corpo é tratado enquanto "corpo rascunho", que pode ser modelado e remodelado a gosto de seu "proprietário" no intuito de alcançar o modelo ideal. Diante deste contexto, temos por objetivo, neste artigo, refletir criticamente sobre as diversas facetas desse paradoxo expresso na atual modalidade de "bem-estar" / "mal-estar", com ênfase em seus "excessos". Adotamos por eixo teórico-metodológico o referencial da Escola de Frankfurt (Teoria Crítica), além de dados coletados em uma pesquisa acerca dos distúrbios de auto-imagem: anorexia, bulimia e vigorexia. Essa investigação qualitativa, realizada em blogs e sites de relacionamentos Orkut de sujeitos portadores dos referidos distúrbios, nos forneceu dados relativos às contemporâneas formas de disciplina/controle, exclusão social/auto-confinamento e metamorfoses corporais, além de depoimentos significativos vinculados à influência das novas tecnologias e dos modelos midiáticos na busca pelo corpo idealizado do consumo. A análise teórico-crítica apontou para a não existência de um verdadeiro paradoxo, mas sim de um excesso de obediência às normas ideais do corpo padrão, que, quando não cumpridas, engendram sentimentos de fracasso e "mal-estar". O "bem-estar" transmuta-se, pois, em "mal-estar", numa jornada em que o prazer se associa ao esforço, o sucesso ao controle e a perfeição ao sofrimento.

In the hypermodern times, in which the "compliment to moderation and cult of the excess" (Lipovetsky, 2004) paradox reigns, the bodies increasingly attract visibility and are dealt with as merchandise to be sold by the cultural industry and health industry. Not rarely does the publicity create an impression that a perfect body, diffused as synonymous of health, well-being and happiness is achievable by all as long as the right instructions and prescriptions are followed./ This way, the body is seen as a "sketch body", which can be remodeled over and over according to the "owner's" taste in order to reach the ideal model. Considering this context, the objective of this article is to reflect critically on the different aspects of this paradox, which are expressed in the current modality of wellness/ discomfort, with emphasis in its excesses and having the Frankfurt school (Critical Theory) as a reference for our theoretic-methodological axis. The collected data from a research on self-image disorders as bulimia, anorexia and overtraining is also examined. This qualitative investigation was executed in blogs, network relationship sites (Orkut) of individuals who carry the referred disorders and it has provided us information related to the contemporaneous ways of discipline/control, social exclusion/ self-confinement, corporal metamorphosis as well as significant testimonials linked to the influence of the new technologies and the media models in a quest/ for the idealized body of consumption. The theoretic-critical analysis undertaken/ has pointed out to the non-existence of a true paradox, whereas to an "excessive" obedience to the ideal norms of a standard body, and when not followed they generate feelings of failure and "discomfort". The "wellness"(well-being) is transmuted into "discomfort", in a journey/ in which associates pleasure to effort, success to control and "perfection" to suffering (pain).

O corpo idealizado de consumo: paradoxos da hipermodernidade / The idealized body of consumption: hypermodernity paradoxes

Em tempos hipermodernos, nos quais reina o paradoxo do "elogio da moderação e da cultura do excesso" (Lipovetsky, 2004), os corpos, cada vez mais, ganham visibilidade e são tratados enquanto mercadoria a ser vendida pela indústria cultural e pela indústria da saúde. A publicidade, não raro, dá a impressão de que um corpo perfeito, veiculado como sinônimo de saúde, bem-estar e felicidade, está ao alcance de todos, desde que seguidas as devidas prescrições. Assim, o corpo é tratado enquanto "corpo rascunho", que pode ser modelado e remodelado a gosto de seu "proprietário" no intuito de alcançar o modelo ideal. Diante deste contexto, temos por objetivo, neste artigo, refletir criticamente sobre as diversas facetas desse paradoxo expresso na atual modalidade de "bem-estar" / "mal-estar", com ênfase em seus "excessos". Adotamos por eixo teórico-metodológico o referencial da Escola de Frankfurt (Teoria Crítica), além de dados coletados em uma pesquisa acerca dos distúrbios de auto-imagem: anorexia, bulimia e vigorexia. Essa investigação qualitativa, realizada em blogs e sites de relacionamentos Orkut de sujeitos portadores dos referidos distúrbios, nos forneceu dados relativos às contemporâneas formas de disciplina/controle, exclusão social/auto-confinamento e metamorfoses corporais, além de depoimentos significativos vinculados à influência das novas tecnologias e dos modelos midiáticos na busca pelo corpo idealizado do consumo. A análise teórico-crítica apontou para a não existência de um verdadeiro paradoxo, mas sim de um excesso de obediência às normas ideais do corpo padrão, que, quando não cumpridas, engendram sentimentos de fracasso e "mal-estar". O "bem-estar" transmuta-se, pois, em "mal-estar", numa jornada em que o prazer se associa ao esforço, o sucesso ao controle e a perfeição ao sofrimento.(AU)

In the hypermodern times, in which the "compliment to moderation and cult of the excess" (Lipovetsky, 2004) paradox reigns, the bodies increasingly attract visibility and are dealt with as merchandise to be sold by the cultural industry and health industry. Not rarely does the publicity create an impression that a perfect body, diffused as synonymous of health, well-being and happiness is achievable by all as long as the right instructions and prescriptions are followed./ This way, the body is seen as a "sketch body", which can be remodeled over and over according to the "owner's" taste in order to reach the ideal model. Considering this context, the objective of this article is to reflect critically on the different aspects of this paradox, which are expressed in the current modality of wellness/ discomfort, with emphasis in its excesses and having the Frankfurt school (Critical Theory) as a reference for our theoretic-methodological axis. The collected data from a research on self-image disorders as bulimia, anorexia and overtraining is also examined. This qualitative investigation was executed in blogs, network relationship sites (Orkut) of individuals who carry the referred disorders and it has provided us information related to the contemporaneous ways of discipline/control, social exclusion/ self-confinement, corporal metamorphosis as well as significant testimonials linked to the influence of the new technologies and the media models in a quest/ for the idealized body of consumption. The theoretic-critical analysis undertaken/ has pointed out to the non-existence of a true paradox, whereas to an "excessive" obedience to the ideal norms of a standard body, and when not followed they generate feelings of failure and "discomfort". The "wellness"(well-being) is transmuted into "discomfort", in a journey/ in which associates pleasure to effort, success to control and "perfection" to suffering (pain).(AU)