Current status and future trends of microbial and nematode-based biopesticides for biocontrol of crop pathogens.

The increasing public demand to avoid the use of synthetic pesticides and fertilizers in agricultural production systems, causing serious environmental damages, has challenged industry to develop new and effective solutions to manage and control phytopathogens. Biopesticides, particularly microbial-based biopesticides, are a promising new alternative with high biodegradability, specificity, suitability for incorporation into integrated pest management practices, low likelihood of resistance development, and practically no known human health risks. However: expensive production methods, narrow action spectra, susceptibility to environmental conditions, short shelf life, poor storage stability, legislation registry constraints, and general lack of knowledge are slowing down their adoption. In addition to regulatory framework revisions and improved training initiatives, improved preservation methods, thoughtfully designed formulations, and field test validations are needed to offer new microbial- and nematode-based biopesticides with improved efficacy and increased shelf-life. During the last several years, substantial advancements in biopesticide production have been developed. The novelty part of this review written in 2023 is to summarize (i) mechanisms of action of beneficial microorganisms used to increase crop performance and (ii) successful formulation including commercial products for the biological control of phytopathogens based on microorganisms, nematode and/or metabolites.

Statistical Experimental Design as a New Approach to Optimize a Solid-State Fermentation Substrate for the Production of Spores and Bioactive Compounds from Trichoderma asperellum.

Managing organic agricultural wastes is a challenge in today's modern agriculture, where the production of different agricultural goods leads to the generation of large amounts of waste, for example, olive pomace and vine shoot in Mediterranean Europe. The discovery of a cost-effective and environment-friendly way to valorize such types of waste in Mediterranean Europe is encouraged by the European Union regulation. As an opportunity, organic agricultural waste could be used as culture media for solid-state fermentation (SSF) for fungal strains. This methodology represents a great opportunity to produce secondary metabolites like 6-pentyl-alpha-pyrone (6-PP), a lactone compound with antifungal properties against phytopathogens, produced by Trichoderma spp. Therefore, to reach adequate yields of 6-PP, lytic enzymes, and spores, optimization using specific agricultural cheap local wastes from Southeastern France is in order. The present study was designed to show the applicability of an experimental admixture design to find the optimal formulation that favors the production of 6-PP. To conclude, the optimized formulation of 6-PP production by Trichoderma under SSF contains 18% wheat bran, 23% potato flakes, 20% olive pomace, 14% olive oil, 24% oatmeal, and 40% vine shoots.

Using bibliometrics to analyze the state of art of pesticide use in vineyard agrosystems: a review.

The production of wine dates to ancient civilization and has heavily influenced different landscapes around the globe. Nowadays, wine is still an important sector in terms of land use and income source in many countries, more specifically in the European Mediterranean region. However, to control pests and regulate wine growth, the extensive use of pesticides is common. The effects and persistence of agrochemicals in the environment is well known and defined in scientific literature as well as the environmental and human health risks of these compounds. The purpose of this review was to do a state of art of worldwide production of academic literature using bibliometric principles and analysis to identify thematic areas of this type of agriculture, and its challenges in a changing world. For this review, the focus is on current practices to see what may evolve for more sustainable viticulture. It was found that the three main producers of wine, i.e., Spain, France, and Italy, are also three top producers of scientific literature on this topic. The use of bibliometric methodologies defined the main thematic areas on this subject as follows: soil-plant transfer of agrochemicals, heavy metal accumulation in vineyard soils, management practices in viticulture, water pollution transport, effects of viticulture on the living part of soil, sustainable alternatives to pesticides. We found that Spain, France, and Italy work the most on developing sustainable alternatives for agricultural practices; however, sustainable alternatives and practices were also the least developed thematic in general.