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1.
mBio ; 13(1): e0381421, 2021 02 22.
Artigo em Inglês | MEDLINE | ID: mdl-35100864

RESUMO

Bacteria have to process several levels of gene regulation and coordination of interconnected regulatory networks to ensure the most adequate cellular response to specific growth conditions. Especially, expression of complex and costly fitness and pathogenicity-associated traits is coordinated and tightly regulated at multiple levels. We studied the interconnected regulation of the expression of the colibactin and yersiniabactin polyketide biosynthesis machineries, which are encoded by two pathogenicity islands found in many phylogroup B2 Escherichia coli isolates. Comparative phenotypic and genotypic analyses identified the BarA-UvrY two-component system as an important regulatory element involved in colibactin and yersiniabactin expression. The carbon storage regulator (Csr) system controls the expression of a wide range of central metabolic and virulence-associated traits. The availability of CsrA, the key translational regulator of the Csr system, depends on BarA-UvrY activity. We employed reporter gene fusions to demonstrate UvrY- and CsrA-dependent expression of the colibactin and yersiniabactin determinants and confirmed a direct interaction of CsrA with the 5' untranslated leader transcripts of representative genes of the colibactin and yersiniabactin operons by RNA electrophoretic mobility shift assays. This posttranscriptional regulation adds an additional level of complexity to control mechanisms of polyketide expression, which is also orchestrated at the level of ferric uptake regulator (Fur)-dependent regulation of transcription and phosphopantetheinyl transferase-dependent activation of polyketide biosynthesis. Our results emphasize the interconnection of iron- and primary metabolism-responsive regulation of colibactin and yersiniabactin expression by the fine-tuned action of different regulatory mechanisms in response to variable environmental signals as a prerequisite for bacterial adaptability, fitness, and pathogenicity in different habitats. IMPORTANCE Secondary metabolite expression is a widespread strategy among bacteria to improve their fitness in habitats where they constantly compete for resources with other bacteria. The production of secondary metabolites is associated with a metabolic and energetic burden. Colibactin and yersiniabactin are two polyketides, which are expressed in concert and promote the virulence of different enterobacterial pathogens. To maximize fitness, they should be expressed only in microenvironments in which they are required. Accordingly, precise regulation of colibactin and yersiniabactin expression is crucial. We show that the expression of these two polyketides is also interconnected via primary metabolism-responsive regulation at the posttranscriptional level by the CsrA RNA-binding protein. Our findings may help to optimize (over-)expression and further functional characterization of the polyketide colibactin. Additionally, this new aspect of concerted colibactin and yersiniabactin expression extends our knowledge of conditions that favor the expression of these virulence- and fitness-associated factors in different Enterobacterales members.


Assuntos
Proteínas de Escherichia coli , Escherichia coli , Policetídeos , Escherichia coli/genética , Escherichia coli/metabolismo , Proteínas de Escherichia coli/genética , Proteínas de Escherichia coli/metabolismo , Regulação Bacteriana da Expressão Gênica , Proteínas de Membrana/metabolismo , Fosfotransferases/genética , Policetídeos/metabolismo , Proteínas Repressoras/genética , Proteínas de Ligação a RNA/genética
2.
mSphere ; 5(4)2020 07 15.
Artigo em Inglês | MEDLINE | ID: mdl-32669458

RESUMO

Colibactin is a nonribosomal peptide/polyketide hybrid natural product expressed by different members of the Enterobacteriaceae which can be correlated with induction of DNA double-strand breaks and interference with cell cycle progression in eukaryotes. Regulatory features of colibactin expression are only incompletely understood. We used Escherichia coli strain M1/5 as a model to investigate regulation of expression of the colibactin determinant at the transcriptional level and to characterize regulatory elements located within the colibactin pathogenicity island itself. We measured clbR transcription in vitro and observed that cultivation in defined minimal media led to increased colibactin expression relative to rich media. Transcription of clbR directly responds to iron availability. We also characterized structural DNA elements inside the colibactin determinant involved in ClbR-dependent regulation, i.e., ClbR binding sites and a variable number of tandem repeats located upstream of clbR We investigated the impact of clbR overexpression or deletion at the transcriptome and proteome levels. Moreover, we compared global gene regulation under these conditions with that occurring upon overexpression or deletion of clbQ, which affects the flux of colibactin production. Combining the results of the transcriptome and proteome analyses with indirect measurements of colibactin levels by cell culture assays and an approximate quantification of colibactin via the second product of colibactin cleavage from precolibactin, N-myristoyl-d-asparagine, we demonstrate that the variable number of tandem repeats plays a significant regulatory role in colibactin expression. We identify ClbR as the only transcriptional activator known so far that is specific and essential for efficient regulation of colibactin production.IMPORTANCE The nonribosomal peptide/polyketide hybrid colibactin can be considered a bacterial virulence factor involved in extraintestinal infection and also a procarcinogen. Nevertheless, and despite its genotoxic effect, colibactin expression can also inhibit bacterial or tumor growth and correlates with probiotic anti-inflammatory and analgesic properties. Although the biological function of this natural compound has been studied extensively, our understanding of the regulation of colibactin expression is still far from complete. We investigated in detail the role of regulatory elements involved in colibactin expression and in the growth conditions that promote colibactin expression. In this way, our data shed light on the regulatory mechanisms involved in colibactin expression and may support the expression and purification of this interesting nonribosomal peptide/polyketide hybrid for further molecular characterization.


Assuntos
Proteínas de Escherichia coli/genética , Escherichia coli/genética , Regulação Bacteriana da Expressão Gênica , Peptídeos/genética , Ativação Transcricional , Escherichia coli/metabolismo , Proteínas de Escherichia coli/metabolismo , Ferro/metabolismo , Peptídeos/metabolismo , Policetídeos/metabolismo
4.
FEMS Microbiol Lett ; 363(20)2016 10.
Artigo em Inglês | MEDLINE | ID: mdl-27754855

RESUMO

Corynebacterium glutamicum is able to metabolize different nitrogen and carbon sources. In standard minimal media, ammonium and urea typically serve as nitrogen source and glucose or sucrose as carbon and energy source; however, amino acids might also play a role as nitrogen, carbon and energy source. In this study, the function of the putative glutaminase GlsK was investigated. A glsK deletion strain showed impaired growth with L-glutamine as carbon and energy source, while growth was improved upon glsK overexpression. GlsK possesses a carboxy-terminal domain that seems to be restricted to Corynebacterium species. A truncated GlsK lacking this extension led to faster growth, indicating a regulatory function of this domain. In fact, GlsK activity is regulated in a pH-dependent manner depending on the carboxy-terminal extension, and is positively influenced by cAMP. Furthermore, the C-terminal extension seems to be important for oligomerization of GlsK.


Assuntos
Compostos de Amônio/metabolismo , Corynebacterium glutamicum/enzimologia , Corynebacterium glutamicum/crescimento & desenvolvimento , Glutaminase/genética , Glutamina/metabolismo , Corynebacterium glutamicum/genética , Corynebacterium glutamicum/metabolismo , Deleção de Genes , Genoma Bacteriano/genética , Glutaminase/metabolismo
5.
Infect Immun ; 84(12): 3358-3368, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-27620723

RESUMO

The genotoxin colibactin is a secondary metabolite produced by a variety of pathogenic enterobacteria. Its biosynthesis requires the enzymatic activity of the phosphopantetheinyl transferase (PPTase) ClbA. We previously showed that ClbA can also contribute to the production of siderophores. Because the biosynthesis of siderophores is regulated by iron availability, we hypothesized that iron could also modulate the production of colibactin through the transcriptional regulation of clbA This study revealed an increased transcription of clbA under iron-limiting conditions and a decrease of clbA expression in iron-rich media. We demonstrate that clbA transcription is regulated by both the ferric uptake regulator (Fur) and the small regulatory noncoding RNA RyhB. We evidenced that the regulation of the transcription of clbA by Fur and RyhB leads to the regulation of colibactin production. This work highlights the complex mechanism of regulation of an important virulence factor by the two major regulators of bacterial iron homeostasis, making iron a key environmental factor contributing to bacterial virulence and carcinogenesis.


Assuntos
Escherichia coli/metabolismo , Regulação Bacteriana da Expressão Gênica/efeitos dos fármacos , Homeostase/fisiologia , Ferro/metabolismo , Peptídeos/metabolismo , Policetídeos/metabolismo , Proteínas de Bactérias/metabolismo , Sequência de Bases , Ferro/farmacologia , RNA Bacteriano
6.
Microb Biotechnol ; 6(2): 196-201, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-22938655

RESUMO

Fluorescent reporter proteins are widely used for the non-invasive monitoring of gene expression patterns, but dynamic measurements are hampered by the extremely high stability of GFP and homologue proteins. In this study, we used SsrA-mediated peptide tagging for the construction of unstable variants of the GFP derivative eYFP (enhanced yellow fluorescent protein) and applied those for transient gene expression analysis in the industrial platform organism Corynebacterium glutamicum.


Assuntos
Proteínas de Bactérias/genética , Biotecnologia/métodos , Corynebacterium glutamicum/metabolismo , Regulação Bacteriana da Expressão Gênica , Variação Genética , Proteínas Luminescentes/genética , Estabilidade Proteica , Sequência de Aminoácidos , Proteínas de Bactérias/química , Proteínas de Bactérias/metabolismo , Corynebacterium glutamicum/genética , Genes Reporter , Vetores Genéticos , Proteínas Luminescentes/química , Proteínas Luminescentes/metabolismo , Dados de Sequência Molecular , Regiões Promotoras Genéticas , Proteínas de Ligação a RNA/química , Proteínas de Ligação a RNA/genética , Proteínas de Ligação a RNA/metabolismo
7.
Appl Microbiol Biotechnol ; 89(2): 239-48, 2011 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-20922371

RESUMO

Nitrogen is one of the macronutrients necessary for living cells, and consequently, assimilation of nitrogen is a crucial step for metabolism. To satisfy their nitrogen demand and to ensure a sufficient nitrogen supply even in situations of nitrogen limitation, microorganisms have evolved sophisticated uptake and assimilation mechanisms for different nitrogen sources. This mini-review focuses on nitrogen metabolism and its control in the biotechnology workhorse Corynebacterium glutamicum, which is used for the industrial production of more than 2 million tons of L: -amino acids annually. Ammonium assimilation and connected control mechanisms on activity and transcription level are summarized, and the influence of mutations on amino acid pools and production is described with emphasis on L: -glutamate, L: -glutamine, and L: -lysine.


Assuntos
Aminoácidos/metabolismo , Corynebacterium glutamicum/metabolismo , Regulação Bacteriana da Expressão Gênica , Engenharia Genética , Nitrogênio/metabolismo , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Corynebacterium glutamicum/genética , Microbiologia Industrial
8.
Microbiology (Reading) ; 156(Pt 10): 3180-3193, 2010 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-20656783

RESUMO

Corynebacterium glutamicum, a Gram-positive soil bacterium employed in the industrial production of various amino acids, is able to use a number of different nitrogen sources, such as ammonium, urea or creatinine. This study shows that l-glutamine serves as an excellent nitrogen source for C. glutamicum and allows similar growth rates in glucose minimal medium to those in ammonium. A transcriptome comparison revealed that the nitrogen starvation response was elicited when glutamine served as the sole nitrogen source, meaning that the target genes of the global nitrogen regulator AmtR were derepressed. Subsequent growth experiments with a variety of mutants defective in nitrogen metabolism showed that glutamate synthase is crucial for glutamine utilization, while a putative glutaminase is dispensable under the experimental conditions used. The gltBD operon encoding the glutamate synthase is a member of the AmtR regulon. The observation that the nitrogen starvation response was elicited at high intracellular l-glutamine levels has implications for nitrogen sensing. In contrast with other Gram-positive and Gram-negative bacteria such as Bacillus subtilis, Salmonella enterica serovar Typhimurium and Klebsiella pneumoniae, a drop in glutamine concentration obviously does not serve as a nitrogen starvation signal in C. glutamicum.


Assuntos
Proteínas de Bactérias/metabolismo , Corynebacterium glutamicum/metabolismo , Glutamina/metabolismo , Nitrogênio/metabolismo , Regulon , Proteínas Repressoras/metabolismo , Proteínas de Bactérias/genética , Corynebacterium glutamicum/genética , Corynebacterium glutamicum/crescimento & desenvolvimento , Meios de Cultura , Perfilação da Expressão Gênica , Regulação Bacteriana da Expressão Gênica , Glutamato Sintase/metabolismo , Mutação , Análise de Sequência com Séries de Oligonucleotídeos , Compostos de Amônio Quaternário/metabolismo , RNA Bacteriano/genética , Proteínas Repressoras/genética
9.
J Biotechnol ; 145(3): 244-52, 2010 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-19963018

RESUMO

Adenylyltransferases regulate glutamine synthetase activity in enterobacteria and actinomycetes such as Streptomyces coelicolor, Mycobacterium tuberculosis and Corynebacterium glutamicum. In this study the effects of a mutation of the glnE gene, coding for adenylyltransferase, on transcriptome and metabolome profiles of C. glutamicum was investigated. As expected, the glnE deletion led to a loss of activity regulation of glutamine synthetase. Astonishingly, additionally the glnE mutation caused a nitrogen limitation response on the transcript level as well. Interestingly, induction of the nitrogen starvation response in the mutant strain was unusually weak and GlnK was present in adenylylated form even without nitrogen starvation. The results obtained might hint to a moonlighting function of adenylyltransferase and might be explained by protein interaction of adenylyltransferase and an unknown interaction partner of the nitrogen regulatory network.


Assuntos
Corynebacterium glutamicum/enzimologia , Nitrogênio/deficiência , Nucleotidiltransferases/metabolismo , Corynebacterium glutamicum/genética , Corynebacterium glutamicum/crescimento & desenvolvimento , Cromatografia Gasosa-Espectrometria de Massas , Deleção de Genes , Perfilação da Expressão Gênica , Regulação Bacteriana da Expressão Gênica , Genes Bacterianos/genética , Teste de Complementação Genética , Metaboloma/genética , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Transdução de Sinais , Transcrição Gênica
10.
J Biotechnol ; 140(1-2): 68-74, 2009 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-19041910

RESUMO

The effects of a deletion of the amtR gene, encoding the master regulator of nitrogen control in Corynebacterium glutamicum, were investigated by metabolome and transcriptome analyses. Compared to the wild type, different metabolite patterns were observed in respect to glycolysis, pentose phosphate pathway, citric acid cycle, and most amino acid pools. Not all of these alterations could be attributed to changes at the level of mRNA and must be caused by posttranscriptional regulatory processes. However, subsequently carried out transcriptome analyses, which were confirmed by gel retardation experiments, revealed two new targets of AmtR, the dapD gene, encoding succinylase involved in m-diaminopimelate synthesis, and the mez gene, coding for malic enzyme. The regulation of dapD connects the AmtR-dependent nitrogen control with l-lysine biosynthesis, the regulation of mez with carbon metabolism. An increased l-glutamine pool in the amtR mutant compared to the wild type was correlated with deregulated expression of the AmtR-regulated glnA gene and an increased glutamine synthetase activity. The glutamate pool was decreased in the mutant and also glutamate excretion was impaired.


Assuntos
Proteínas de Bactérias/genética , Corynebacterium glutamicum/genética , Perfilação da Expressão Gênica , Metabolômica , Proteínas Repressoras/genética , Aciltransferases/genética , Aciltransferases/metabolismo , Proteínas de Bactérias/metabolismo , Sequência de Bases , Corynebacterium glutamicum/metabolismo , Ensaio de Desvio de Mobilidade Eletroforética , Cromatografia Gasosa-Espectrometria de Massas , Deleção de Genes , Glutamato-Amônia Ligase/genética , Glutamato-Amônia Ligase/metabolismo , Ácido Glutâmico/metabolismo , Dados de Sequência Molecular , Processamento Pós-Transcricional do RNA , Proteínas Repressoras/metabolismo
11.
J Bacteriol ; 189(16): 5903-15, 2007 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-17557815

RESUMO

We present a comprehensive analysis of carbohydrate uptake systems of the soil bacterium Mycobacterium smegmatis and the human pathogen Mycobacterium tuberculosis. Our results show that M. smegmatis has 28 putative carbohydrate transporters. The majority of sugar transport systems (19/28) in M. smegmatis belong to the ATP-binding cassette (ABC) transporter family. In contrast to previous reports, we identified genes encoding all components of the phosphotransferase system (PTS), including permeases for fructose, glucose, and dihydroxyacetone, in M. smegmatis. It is anticipated that the PTS of M. smegmatis plays an important role in the global control of carbon metabolism similar to those of other bacteria. M. smegmatis further possesses one putative glycerol facilitator of the major intrinsic protein family, four sugar permeases of the major facilitator superfamily, one of which was assigned as a glucose transporter, and one galactose permease of the sodium solute superfamily. Our predictions were validated by gene expression, growth, and sugar transport analyses. Strikingly, we detected only five sugar permeases in the slow-growing species M. tuberculosis, two of which occur in M. smegmatis. Genes for a PTS are missing in M. tuberculosis. Our analysis thus brings the diversity of carbohydrate uptake systems of fast- and a slow-growing mycobacteria to light, which reflects the lifestyles of M. smegmatis and M. tuberculosis in their natural habitats, the soil and the human body, respectively.


Assuntos
Transporte Biológico , Metabolismo dos Carboidratos , Proteínas de Membrana Transportadoras/metabolismo , Mycobacterium smegmatis/genética , Mycobacterium tuberculosis/genética , Sistema Fosfotransferase de Açúcar do Fosfoenolpiruvato/metabolismo , Carboidratos , Regulação Bacteriana da Expressão Gênica , Mycobacterium smegmatis/enzimologia , Mycobacterium smegmatis/fisiologia , Mycobacterium tuberculosis/enzimologia , Mycobacterium tuberculosis/fisiologia
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