RESUMO
An approach for automated nanotomography, a layer-by-layer imaging technique based on scanning probe microscopy (SPM), is presented. Stepwise etching and imaging is done in situ in a liquid cell of an SPM. The flow of etching and rinsing solutions after each etching step is controlled with solenoid valves which allow for an automated measuring protocol. The thermal drift and the drift of the piezo scanner is corrected by applying offsets calculated from the cross correlation coefficients between successive images. As an example, we have imaged human bone with approximately 10 nm resolution using tapping mode SPM and successive etching with hydrochloric acid.
Assuntos
Aumento da Imagem/instrumentação , Microscopia de Varredura por Sonda/instrumentação , Nanoestruturas/ultraestrutura , Nanotecnologia/instrumentação , Robótica/instrumentação , Manejo de Espécimes/instrumentação , Tomografia Óptica/instrumentação , Aumento da Imagem/métodos , Microscopia de Varredura por Sonda/métodos , Nanotecnologia/métodos , Reprodutibilidade dos Testes , Robótica/métodos , Sensibilidade e Especificidade , Manejo de Espécimes/métodos , Tomografia Óptica/métodosRESUMO
The surface structure of a lamellar polystyrene-block-polybutadiene-block-polymethylmethacrylate (SBM) triblock copolymer forms a complex reconstruction, which breaks the two-dimensional continuous translational symmetry of an ideal (homogeneous) SBM surface. Despite the very different types of matter and order, our findings reveal a remarkable analogy with the well-known phenomenon of surface reconstruction of single crystals, in particular, with the (2x1) "buckling row" reconstruction of the Si(100) surface. Similarities and differences between both classes of materials are discussed on the basis of symmetry considerations.
