Assuntos
Humanos , Anticorpos Antibacterianos/sangue , Antígenos de Bactérias/imunologia , Antituberculosos/uso terapêutico , Proteínas de Bactérias/imunologia , Busca de Comunicante , Isoniazida/uso terapêutico , Mycobacterium tuberculosis/imunologia , Tuberculose Pulmonar/imunologia , Antibioticoprofilaxia , Fatores de Risco , Teste Tuberculínico , Tuberculose Pulmonar/prevenção & controleRESUMO
A tuberculose (TB) continua sendo um dos mais urgentes problemas de saúde pública do mundo, com 8 a 10 milhões de novos casos e 2 a 3 milhões de mortes a cada ano. Cerca de 50 milhõesde pessoas estão infectadas com o Mycobacterium tuberculosis. A fim de desenvolver testes para imunodiagnóstico da TB, vários antígenos têm sido testados na resposta imune humoral de pacientes com TB ativa. O teste imunoenzimático de ELISA realizado com amostras de plasma de 45 pacientes com tuberculose pulmonar e 172 contatos (96 prova tuberculínica negativa e 76, positiva) foi conduzido para avaliação da resposta imune humoral, com pesquisa de anticorpos das classesIgM e IgG contra o antígeno recombinante GroEs do Mycobacterium tuberculosis (MtGroEs). Pacientes com tuberculose pulmonar ativa apresentaram maiores níveis de IgG anti-rGroEs do que indivíduos saudáveis, o que permitiu a discriminação entre os dois grupos e sugeriu resposta imune humoral específica a este antígeno.
Tuberculosis (TB) remains one of the most important public health problems ofthe world, with 8-10 million new cases and 2-3 million deaths each year. About 50 million people are infected with Mycobacterium tuberculosis. In order to develop immunodiagnostic tests for TB, several antigens have been tested for the humoral immune response of patients with active TB. ELISA was performed with plasma samples from 45 patients with pulmonary TB and 172 contacts (96 negative and 76 positive for the tuberculin skin test). The humoral immune response was evaluated through assessment of IgM and IgG antibodies against recombinant Mycobacterium tuberculosis GroES (MtGroEs). Patients with active pulmonary tuberculosis had higher levels of IgG anti-rGroEs than healthy individuals, allowing discriminationbetween the two groups.
Assuntos
Humanos , Masculino , Feminino , Mycobacterium tuberculosis , Proteínas Recombinantes , Sistema Imunitário , Tuberculose Pulmonar , Ensaio de Imunoadsorção Enzimática , Estudos TransversaisAssuntos
Anticorpos Antibacterianos/sangue , Antígenos de Bactérias/imunologia , Antituberculosos/uso terapêutico , Proteínas de Bactérias/imunologia , Busca de Comunicante , Isoniazida/uso terapêutico , Mycobacterium tuberculosis/imunologia , Tuberculose Pulmonar/imunologia , Antibioticoprofilaxia , Humanos , Fatores de Risco , Teste Tuberculínico , Tuberculose Pulmonar/prevenção & controleRESUMO
Tuberculosis (TB) is one of the oldest human infectious diseases and one third of the world's population is latently infected. Brazil is an endemic area for TB. One of the most important challenges in TB control is the identification of latently infected individuals. Health Care Workers (HCW) are at high risk of being infected with Mycobacterium tuberculosis and even to become TB latently infected. The aim of this study was to increase knowledge about humoral immune response in TB latently infected individuals. HCW were classified according to their tuberculin skin test (TST), as positive or negative. The antibody response to GLcB, MPT51 and HSPX from Mycobacterium tuberculosis was evaluated. TST negative HCW constituted the majority of those who showed a humoral immune response. Antibody levels varied according to antigen characteristics, TST and BCG status. We suggest that possibly the presence of those antibodies could have a function in the protective immune response against Mycobacterium tuberculosis.
Assuntos
Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Antígenos de Bactérias/imunologia , Proteínas de Bactérias/imunologia , Tuberculose Latente/imunologia , Malato Sintase/imunologia , Mycobacterium tuberculosis/imunologia , Recursos Humanos em Hospital , Ensaio de Imunoadsorção Enzimática , Imunoglobulina G/imunologia , Imunoglobulina M/imunologia , Tuberculose Latente/microbiologiaRESUMO
Tuberculosis (TB) is one of the oldest human infectious diseases and one third of the world's population is latently infected. Brazil is an endemic area for TB. One of the most important challenges in TB control is the identification of latently infected individuals. Health Care Workers (HCW) are at high risk of being infected with Mycobacterium tuberculosis and even to become TB latently infected. The aim of this study was to increase knowledge about humoral immune response in TB latently infected individuals. HCW were classified according to their tuberculin skin test (TST), as positive or negative. The antibody response to GLcB, MPT51 and HSPX from Mycobacterium tuberculosis was evaluated. TST negative HCW constituted the majority of those who showed a humoral immune response. Antibody levels varied according to antigen characteristics, TST and BCG status. We suggest that possibly the presence of those antibodies could have a function in the protective immune response against Mycobacterium tuberculosis.
