Functional capacity and risk of injury in CrossFit practitioners measured through smartphone apps.

BACKGROUND: CrossFit is a high intensity functional training that tends to challenge physical limits. The objectives of this study were to assess functional capacity, prevalence and risk of injury in CrossFit practitioners. METHODS: This cross-sectional, observational and prospective study evaluate the rate of injuries that occurred in CrossFit practitioners in the last 12 months and their functional capacities. The sample was given for convenience, with a total of 22 participants. Functional capacities and risk of injury were measured by functional tests using PHAST and Clinometer applications. The prevalence of injuries was cataloged using the Nordic Musculoskeletal Questionnaire. RESULTS: 5% of the injuries occurred in the neck; 9% in shoulder, hip, thighs, ankles and feet; 14% in the lumbar spine and knees. The worst functional results were for the shoulder medial rotation ROM test, where 86-95% of the athletes were classified as "Bad"; the dorsiflexion ROM test also performed poorly in 68% of athletes. CONCLUSION: This study shows that the CrossFit practice suggests that the injury prevalence is relatively low, affecting mainly knees, lumbar spine, wrists and hands. However, the risk of injuries shown by the functional musculoskeletal assessment is higher, especially in the shoulder and ankle, and it is important for the practitioner to realize a specific functional assessment before starting training.

Electrochemical degradation of diclofenac generates unexpected thyroidogenic transformation products: Implications for environmental risk assessment.

Diclofenac (DCF) is an environmentally persistent, nonsteroidal anti-inflammatory drug (NSAID) with thyroid disrupting properties. Electrochemical advanced oxidation processes (eAOPs) can efficiently remove NSAIDs from wastewater. However, eAOPs can generate transformation products (TPs) with unknown chemical and biological characteristics. In this study, DCF was electrochemically degraded using a boron-doped diamond anode. Ultra-high performance liquid chromatography coupled with high-resolution mass spectrometry was used to analyze the TPs of DCF and elucidate its potential degradation pathways. The biological impact of DCF and its TPs was evaluated using the Xenopus Eleutheroembryo Thyroid Assay, employing a transgenic amphibian model to assess thyroid axis activity. As DCF degradation progressed, in vivo thyroid activity transitioned from anti-thyroid in non-treated samples to pro-thyroid in intermediately treated samples, implying the emergence of thyroid-active TPs with distinct modes of action compared to DCF. Molecular docking analysis revealed that certain TPs bind to the thyroid receptor, potentially triggering thyroid hormone-like responses. Moreover, acute toxicity occurred in intermediately degraded samples, indicating the generation of TPs exhibiting higher toxicity than DCF. Both acute toxicity and thyroid effects were mitigated with a prolonged degradation time. This study highlights the importance of integrating in vivo bioassays in the environmental risk assessment of novel degradation processes.

Fast and accurate 3-D spine MRI segmentation using FastCleverSeg.

Accurate and efficient segmenting of vertebral bodies, muscles, and discs is crucial for analyzing various spinal diseases. However, traditional methods are either laborious and time-consuming (manual segmentation) or require extensive training data (fully automatic segmentation). FastCleverSeg, our proposed semi-automatic segmentation approach, addresses those limitations by significantly reducing user interaction while maintaining high accuracy. First, we reduce user interaction by requiring the manual annotation of only two or three slices. Next, we automatically Estimate the Annotation on Intermediary Slices (EANIS) using traditional computer vision/graphics concepts. Finally, our proposed method leverages improved voxel weight balancing to achieve fast and precise volumetric segmentation in the segmentation process. Experimental evaluations on our assembled diverse MRI databases comprising 179 patients (60 male, 119 female), demonstrate a remarkable 25 ms (30 ms standard deviation) processing time and a significant reduction in user interaction compared to existing approaches. Importantly, FastCleverSeg maintains or surpasses the segmentation quality of competing methods, achieving a Dice score of 94%. This invaluable tool empowers physicians to efficiently generate reliable ground truths, expediting the segmentation process and paving the way for future integration with deep learning approaches. In turn, this opens exciting possibilities for future fully automated spine segmentation.

Detailed comparison of in-house developed and commercially available heart-cutting and selective comprehensive two-dimensional liquid chromatography systems.

Recently, two-dimensional liquid chromatography (2D-LC) has become a popular approach to analyze complex samples. This is partly due to the introduction of commercial 2D-LC systems. In the past, 2D-LC was carried out on in-house developed setups, typically consisting of several switching valves and sample loops as the interface between the two dimensions. Commercial systems usually offer different 2D-LC modes in combination with specialized software to operate the instrument and analyze the data. This makes them highly user-friendly, however, at an increased cost compared to in-house developed setups. This study aims to make a comparison between an in-house developed 2D-LC setup and a commercially available 2D-LC instrument. The comparison is made based on experimental differences, in addition to more general differences, including cost price, flexibility, and ease of operation. Special attention is also paid to the different strategies to deal with the mobile phase incompatibility between the highly orthogonal separation mechanisms considered in this work: hydrophilic interaction liquid chromatography (HILIC) and reversed-phase LC (RPLC). For the commercial 2D-LC instrument, this is done using active solvent modulation (ASM), a valve-based approach allowing the on-line dilution of the effluent eluting from the first dimension column before transfer to the second dimension (2D) column. For the in-house developed setup, a combination of restriction capillaries and a trap column is used. Using a sample of 28 compounds with a large polarity range, peak shapes and recoveries of the 2D-chromatograms are compared for both setups. For early eluting compounds, the selective comprehensive approach, currently only possible on the commercial 2D-LC instrument, results in the best peak shapes and recoveries, however, at the cost of an increased analysis time. In general, depending on the analytical goal (single heart-cut versus full-comprehensive 2D-LC), an in-house developed system can be satisfactory for the analysis of specific target compounds/samples. For more complex problems, it can be interesting to use a more specialized commercial 2D-LC instrument. Overall, this comparison study provides advice for analytical scientists, who are considering to use 2D-LC, on the type of equipment to consider, depending on the needs of their particular applications.

Electrochemical degradation of 17α-ethinylestradiol: Transformation products, degradation pathways and in vivo assessment of estrogenic activity.

Conventional water treatment methods are not efficient in eliminating endocrine disrupting compounds (EDCs) in wastewater. Electrochemical Advanced Oxidation Processes (eAOPs) offer a promising alternative, as they electro-generate highly reactive species that oxidize EDCs. However, these processes produce a wide spectrum of transformation products (TPs) with unknown chemical and biological properties. Therefore, a comprehensive chemical and biological evaluation of these remediation technologies is necessary before they can be safely applied in real-life situations. In this study, 17α-ethinylestradiol (EE2), a persistent estrogen, was electrochemically degraded using a boron doped diamond anode with sodium sulfate (Na2SO4) and sodium chloride (NaCl) as supporting electrolytes. Ultra-high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry was used for the quantification of EE2 and the identification of TPs. Estrogenic activity was assessed using a transgenic medaka fish line. At optimal operating conditions, EE2 removal reached over 99.9% after 120 min and 2 min, using Na2SO4 and NaCl, respectively. The combined EE2 quantification and in vivo estrogenic assessment demonstrated the overall estrogenic activity was consistently reduced with the degradation of EE2, but not completely eradicated. The identification and time monitoring of TPs showed that the radical agents readily oxidized the phenolic A-ring of EE2, leading to the generation of hydroxylated and/or halogenated TPs and ring-opening products. eAOP revealed to be a promising technique for the removal of EE2 from water. However, caution should be exercised with respect to the generation of potentially toxic TPs.

Is the Integration between Corn and Grass under Different Sowing Modalities a Viable Alternative for Silage?

This study aimed to evaluate the fermentation pattern and dry-matter losses in corn (Zea mays L.) silage intercropped with Urochloa brizantha cv. Marandu and Megathyrsus maximus cv. Mombasa grasses in different sowing modalities through crop-livestock integration. The experimental design was in randomized blocks, which were arranged in a 2 × 5 factorial scheme with four repetitions. The first factor consisted of the grass cultivars Marandu and Mombasa. The second factor was the sowing modalities of grasses intercropped with corn: (1) simultaneous row sowing and inter-row corn sowing (no fertilizer); (2) simultaneous row sowing and inter-row corn sowing (with fertilizer); (3) simultaneous sowing with double grass row in the corn inter-row; (4) delayed sowing inter-row at 7 days after corn emergence; and (5) delayed sowing inter-row at 14 days after corn emergence. The forage buffer capacity (BC), silage pH and ammoniacal nitrogen (NH3-N) content, forage (FORDM) and silage dry-matter (SILDM) percentages, gas losses (GL), effluent losses (EL), and dry-matter recovery (DMR) parameters on the ensilage were evaluated. Only forage BC, silage NH3-N, and silage DMR variables differed (p < 0.05) from the control silage (monocropped corn) when the integration was carried out. The grass cultivar factors and sowing modalities for BC and NH3-N variables had an effect. The intercropping of corn and Marandu grass or Mombasa grass, in any grass sowing modality, did not affect the quality of the silage.

Epistemological (re)considerations on physical education in brazil from the kinesiology of the united states: a horizon beyond the epithets / (re)considerações epistemológicas sobre a educação física no brasil a partir da cinesiologia dos estados unidos: um horizonte além dos epítetos

ABSTRACT This study discusses theoretical reverberations that stand out in the field of Physical Education (PE) in Brazil in the face of epistemological discussions demarcated within the scope of PE in the USA from the 1960s onwards, and due to the contributions of the Disciplinary Movement and the context by which Kinesiology then established its position as a scope in the field of American PE. An exploratory literature review was carried out with the following guiding question: what is the impact of the epistemological developments of North American PE on the configuration of the general theory of PE in Brazil? From this perspective, we verified the authors in the Brazilian field who took a position on the resonances and limits arising from the conjectures of the scientific field of PE in the USA. Through this investigation, some processes of inhibition were highlighted by which the proposal of Kinesiology could not increase reflections in Brazil, with few exceptions.

RESUMO Este estudo discute reverberações teóricas que se destacaram no campo da Educação Física (EF) no Brasil diante das investigações epistemológicas demarcadas no âmbito da EF nos EUA a partir da década de 1960, e devido às contribuições do Movimento Disciplinar e ao contexto pelo qual a Cinesiologia se estabeleceu então como escopo no campo da EF americana. Foi realizada uma revisão exploratória da literatura com a seguinte questão norteadora: qual impacto dos desenvolvimentos epistemológicos da EF norte-americana na configuração da teoria geral da EF no Brasil? Nessa perspectiva, verificamos os autores do campo brasileiro que adotaram uma posição sobre as ressonâncias e limites decorrentes das conjecturas do campo científico da EF nos EUA. Através desta investigação, foram destacados alguns processos de inibição pelos quais a proposta da Cinesiologia não conseguiu aumentar reflexões no Brasil, com poucas exceções.

A cobertura da Folha de São Paulo dos Jogos Paralímpicos entre 1972 e 2020 / The Folha de São Paulo's coverage of the Paralympic Games between 1972 and 2020 / La cobertura de Folha de São Paulo de los Juegos Paralímpicos entre 1972 y 2020

Este estudo teve como objetivo levantar algumas características da cobertura da Folha de São Paulo dos Jogos Paralímpicos (JP) entre 1972 e 2020. Os objetivos específicos foram: levantar o número de publicações relativas aos JP durante as diferentes edições; mapear a distribuição das publicações por modalidades e tipos de deficiência; levantar os números totais de medalhas conquistadas pelo país a cada edição, por modalidades e por tipos de deficiência, e contrastar estes números com as informações levantadas nos objetivos anteriores. Realizamos uma análise quantitativa e descritiva dos dados. Verificamos que número de publicações cresceu, com algumas oscilações; a deficiência física foi privilegiada nas publicações em relação aos outros tipos de deficiência; as publicações privilegiaram algumas modalidades e tipos de deficiência; e não houve uma correlação significativa entre o número total de publicações e o total de medalhas. Este trabalho oferece subsídios para uma cobertura mais qualificada do paradesporto.

This study aimed to identify some characteristics of Folha de São Paulo's coverage of the Paralympic Games (PG) between 1972 and 2020. The specific objectives were:to determine the number of publications related to the PG during different editions; to map the distribution of publications by sport and type of disability; to determine the total number of medals won by the country in each edition, by sport and by type of disability; and to compare these numbers with the information obtained in the previous objectives. The data analysis was quantitative and descriptive. The number of publications increased, with some fluctuations; physical disability was privileged in the publications compared to other types of disabilities; publications favored some sports and types of disability; and there was no significant correlation between the total number of publications and the total number of medals. This study provides support for a more qualified coverage of para sports.

Este estudio tuvo como objetivo recopilar algunas características de la cobertura de la Folha de São Paulo de los Juegos Paralímpicos (JP) entre 1972 y 2020. Los objetivos específicos fueron: recopilar el número de publicaciones relacionadas con los JP; mapear la distribución de las publicaciones por modalidades y tipos de discapacidad; recopilar los números totales de medallas conquistadas por el país en cada edición, por modalidades y por tipos de discapacidad, y contrastar estos números con la información recopilada en los objetivos anteriores. El análisis fue cuantitativo y descriptivo. El número de publicaciones aumentó, con algunas fluctuaciones; la discapacidad física fue privilegiada en las publicaciones en relación con otros tipos de discapacidad; las publicaciones privilegiaron algunas modalidades y tipos de discapacidad; y no hubo una correlación significativa entre el número total de publicaciones y el total de medallas. Este trabajo ofrece subsidios para una cobertura más calificada del paradesporto.

Assessment of behavior-related pain in school teachers during emergency remote teaching: cross-sectional study / Avaliação de dor relacionada ao comportamento de professores durante o ensino remoto emergencial: estudo observacional transversal

ABSTRACT BACKGROUND AND OBJECTIVES: In the teacher's professional practice, there is a high level of stress, anxiety and incidence of pain. With the advent of COVID-19 and the emergence of remote teaching, it is possible that this scenario has been aggravated. The objective of this study was to evaluate chronic pain, central sensitization and pain catastrophizing among primary education teachers during remote teaching offered due to the pandemic. METHODS: A cross-sectional analytical observational study. Participants were 200 teachers from different regions of Brazil who responded through an online form a sociodemographic questionnaire and a questionnaire about their working conditions. In addition, pain intensity was assessed using the Visual Analog Pain Scale (VAS), central sensitization state was assessed using the Central Sensitization Questionnaire (CSI), and pain catastrophizing was assessed using the Catastrophic Thinking Scale about Pain (PCS). RESULTS: Most of the teachers were female, white, from the southeast region, with lato sensu specialization, had an income of 2 to 2.5 minimum wages, working 21 to 40 hours a week. Most of the teachers reported pain intensity equal to eight and the body region most affected was the lumbar spine. It was observed that teachers with low salary, uncomfortable environment and longer days dedicated to remote teaching showed a greater tendency to central sensitization and pain catastrophizing. Most teachers reported pain intensity equal to eight and the most affected body region was the lumbar spine. Teachers with uncomfortable furniture reported increased pain, especially in the lumbar spine and neck. They also showed higher levels of central sensitization to pain, inversely proportional to their salary income and, in sum, teachers with a greater feeling of discomfort catastrophized more which reflects the physical and emotional damage that pain can cause. CONCLUSION: Remote teaching during the pandemic of COVID-19 impacted physical and emotional changes in teachers of primary education. The professionals perceived that their furniture was not the most adequate for the high amount of time they had to work on academic activities using computers (in general, over 40 hours a week), they reported increased pain, especially in the lumbar spine and neck, they presented higher levels of central pain sensitization, which was influenced by low salary income, and, in sum, teachers with higher feelings of discomfort catastrophized more, which reflects the physical and emotional damage that pain may cause. All these affections tend to reduce the teachers' quality of life and, consequently, affect the teaching and learning processes.

RESUMO JUSTIFICATIVA E OBJETIVOS: Na atuação profissional do professor, há elevado nível de estresse, ansiedade e incidência de dor. Com o advento do COVID-19 e a emergência do ensino remoto, é possível que este cenário tenha sido agravado. O objetivo deste estudo foi avaliar quadros de dor crônica, sensibilização central e catastrofização da dor em professores da rede básica durante o ensino remoto ofertado devido à pandemia. MÉTODOS: Trata-se de um estudo observacional analítico do tipo transversal. Participaram 200 professores de diferentes regiões do Brasil que responderam, através de um formulário online, perguntas sobre aspectos sociodemográficos e suas condições de trabalho, além de serem avaliadas a intensidade de dor por meio da escala analógica visual de dor, o estado de sensibilização central por meio do Questionário de Sensibilização Central (CSI) e a catastrofização da dor por meio da Escala de Pensamento Catastrófico sobre a Dor. RESULTADOS: A maioria dos professores eram do sexo feminino, brancos, da região sudeste, com especialização lato sensu, apresentavam renda de 2 a 2,5 salários-mínimos e jornada de trabalho de 21 a 40 horas semanais. A maioria dos professores relatou intensidade de dor igual a oito e a região do corpo mais afetada foi a coluna lombar. Observou-se que professores com baixo salário, ambiente desconfortável e maior jornada dedicada ao ensino remoto apresentaram maior tendência à sensibilização central e à catastrofização da dor. Professores com mobiliário desconfortável relataram aumento da dor, em especial, na coluna lombar e no pescoço. Apresentaram também maiores níveis de sensibilização central à dor, inversamente proporcional à sua renda salarial e, de maneira somatória, professores com maior sensação de desconforto catastrofizaram mais o que reflete os prejuízos físicos e emocionais que a dor pode causar. CONCLUSÃO: O ensino remoto durante a pandemia do COVID-19 impactou alterações físicas e emocionais nos professores da rede básica de ensino. Os profissionais perceberam que seu mobiliário não era o mais adequado para a alta permanência de tempo que tiveram de trabalhar em atividades acadêmicas pelo computador (em geral, acima de 40 horas semanais), relataram aumento da dor, em especial na coluna lombar e no pescoço, apresentaram maiores níveis de sensibilização central à dor, que foi influenciado por baixa renda salarial e, de maneira somatória, professores com maior sensação de desconforto catastrofizaram mais, o que reflete os prejuízos físicos e emocionais que a dor pode causar. Todos estes acometimentos tendem a reduzir a qualidade de vida do professor e, consequentemente, afetar o processo de ensino e aprendizagem.

COVID-19 cross-sectional study in Maricá, Brazil: The impact of vaccination coverage on viral incidence.

Population surveillance in COVID-19 Pandemic is crucial to follow up the pace of disease and its related immunological status. Here we present a cross-sectional study done in Maricá, a seaside town close to the city of Rio de Janeiro, Brazil. Three rounds of study sampling, enrolling a total of 1134 subjects, were performed during May to August 2021. Here we show that the number of individuals carrying detectable IgG antibodies and the neutralizing antibody (NAb) levels were greater in vaccinated groups compared to unvaccinated ones, highlighting the importance of vaccination to attain noticeable levels of populational immunity against SARS-CoV-2. Moreover, we found a decreased incidence of COVID-19 throughout the study, clearly correlated with the level of vaccinated individuals as well as the proportion of individuals with detectable levels of IgG anti-SARS-CoV-2 and NAb. The observed drop occurred even during the introduction of the Delta variant in Maricá, what suggests that the vaccination slowed down the widespread transmission of this variant. Overall, our data clearly support the use of vaccines to drop the incidence associated to SARS-CoV-2.

Go tani and his influence on the kinesiology field in the brazilian scientific context / Go tani e sua influência no campo da cinesiologia no contexto científico brasileiro

ABSTRACT This study aimed to group some perspectives on Go Tani's epistemological activity in the context of Brazilian Physical Education (PE), establishing his contributions and reflections on Kinesiology for the structuring of undergraduate and graduate Physical Education in Brazil. This is a bibliographical and exploratory study, which prioritized Tani's production on the most varied platforms and the materials that circulated about Kinesiology in national and international literature. Finally, through the theoretical review, it was possible to identify that the PE field is a scenario full of disputes and that even with the transformations of paradigms that infer the legitimate alternatives in the scientific context, the relationships that are established between the different schools of PE thought have erupted in a process of mutual incomprehension that contributes to the circularity of perspectives and the maintenance of beliefs in the field.

RESUMO O presente estudo objetivou agrupar algumas perspectivas sobre a atividade epistemológica de Go Tani no contexto da Educação Física (EF) brasileira, estabelecendo suas contribuições e reflexões a partir da Cinesiologia para a estruturação da graduação e da pós-graduação em Educação Física no Brasil. Trata-se de um estudo bibliográfico e exploratório, o qual priorizou a produção de Tani nas mais variadas plataformas, bem como os materiais que circularam sobre a Cinesiologia na literatura nacional e internacional. Por fim, através da referida revisão teórica, foi possível identificar que o campo da EF é um cenário repleto de disputas e que mesmo com as transformações dos paradigmas que inferem nas alternativas legítimas no contexto científico, as relações que são estabelecidas entre as distintas escolas de pensamento da EF têm eclodido num processo de interincompreensão que contribui com a circularidade de perspectivas e a manutenção de crenças no campo.

Rumen fermentation and metabolic profile of rams fed with diets amended cottonseed cake.

The aim of the study was to evaluate the addition of increasing cottonseed cake contents in the diet. The diets were composed of corn silage (500 g kg-1) and concentrate feed (500 g kg-1) on a dry matter (DM) basis. The treatments consisted of the substitution of 0, 140, 280, and 420 g kg-1 soybean meal to cottonseed cake in the DM of concentrate feed. Four rams of the Santa Inês breed, average age of 12 months and average weight of 27.77 ± 3.87 kg, were distributed in a Latin square design (4 × 4) in a split-split-plot design, with diets in the plots and as subplots were 5 days of collection and the collection times. The results were subjected to analysis of variance and regression at 5% probability. The soybean meal substitution contents by cottonseed cake in the concentrate increased linearly the pH (P = 0.019). Ruminal ammonia-nitrogen concentration decreased linearly by 0.0137 mg dL-1 at each 10 g/kg of substitution and the time after feeding promoted the same effect (P < 0.001) decreased linearly by 0.6204 mg dL-1 at each hour after feeding. There was an increased linearly of 0.0116 mmol mL-1 at the concentration of propionic (P = 0.008) and a reduction of 0.0062 mmol mL-1 at the concentration of butyric (P = 0.009) with cottonseed cake content; however, the substitution did not influence the others short-chain fatty acids. Replacement of soybean meal with cottonseed cake in the sheep feed did not significantly alter the blood parameters, promoting minor changes in the ruminal parameters mainly in the ruminal ammonia-nitrogen without affecting the animal's health.

Prevalência de quedas entre idosos de uma instituição de longa permanência / Prevalence of falls among the elderly in a long-term institution

Objetivo: analisar a prevalência bem como as associações entre as variáveis independentes relacionadas às quedas dos idosos. Método: estudo descritivo, quantitativo, realizado no ano de 2017 em uma Instituição de Longa Permanência para Idosos, após aprovação pelo Comitê de Ética em Pesquisa sob parecer nº2.152.185. Utilizado questionário elaborado a partir das variáveis de risco para queda conforme o Protocolo de Quedas do Ministério da Saúde e Morse FallScale. Elaborado banco de dados em Excel e realizada análise descritiva e de associação dos dados cálculos das frequências absolutas e relativas, p-valor e teste qui-quadrado. Resultados: a prevalência geral de quedas foi de 48,5%. A polifarmácia foi o fator de risco associado que obteve maior significância. Conclusão: o número de quedas encontrado e fatores associados são preocupantes frente a população idosa estudada ao considerar as quedas como um indicador da qualidade da assistência.(AU)

Objective: to analyze the prevalence as well as the associations between the independent variables related to falls among the elderly. Method: descriptive, quantitative study, carried out in 2017 in a Long Stay Institution for the Elderly, after approval by the Research Ethics Committee under opinion nº 2.152.185. A questionnaire was used based on the risk variables for falls according to the Falls Protocol of the Ministry of Health and Morse FallScale. Prepared an Excel database and performed descriptive analysis and data association, calculations of absolute and relative frequencies, p-value and chi-square test. Results: the overall prevalence of falls was 48.5%. Polypharmacy was the associated risk factor that had the greatest significance. Conclusion: the number of falls found and associated factors are of concern to the elderly population studied when considering falls as an indicator of quality of care.(AU)

Objetivo: analizar la prevalencia y las asociaciones entre las variables independientes relacionadas con las caídas en los ancianos. Método: estudio descriptivo, cuantitativo, realizado en 2017 en una Institución de Larga Estancia de Mayores, previa aprobación del Comité de Ética en Investigación bajo dictamen nº 2.152.185. Se utilizó un cuestionario basado en las variables de riesgo de caídas según el Protocolo de Caídas del Ministerio de Salud y Morse FallScale. Se preparó una base de datos en Excel y se realizó análisis descriptivo y asociación de datos, cálculos de frecuencias absolutas y relativas, valor p y prueba de chi-cuadrado. Resultados: la prevalencia global de caídas fue del 48,5%. La polifarmacia fue el factor de riesgo asociado de mayor significación. Conclusión: el número de caídas encontradas y los factores asociados preocupan a la población anciana estudiada al considerar las caídas como un indicador de la calidad de la atención.(AU)

Sentinel lymph node biopsy using single-agent mapping tracer (blue dye) after neoadjuvant chemotherapy in a Brazilian cohort of breast cancer patients. Real world evidence.

PURPOSE: To reduce false-negative rates (FNR) in sentinel lymph node biopsy (SLNB) of clinically positive (cN+) axilla in patients undergoing neoadjuvant chemotherapy (NAC). The removal of three or more lymph nodes with dual-tracer mapping including a radioisotope was used. However, in the Brazilian Unified Health System, the radioisotope tracer is not feasible in some hospitals. We conducted a cross-sectional study to evaluate the detection rate of sentinel lymph node (SLN) in patients who converted from cN+ to ycN0 after NAC using blue dye as a single-agent mapping tracer. METHODS: During the period of March 2018 to September 2019, 34 patients who underwent NAC with cN+ who converted to ycN0 were enrolled in the study. The SLNB was performed using blue dye as a single-agent mapping followed by axillary lymph node dissection (ALND). RESULTS: The detection rate of sentinel lymph node was of 85.3%, being SLNB not possible for five patients (14.7%), due to fibrosis. The mean number of removed SLN was 2.5. CONCLUSIONS: The use of blue dye as a single-agent mapping tracer demonstrated an acceptable detection rate of 85.3%. Although the FNR was possible to be determined, the small sample size might overestimate this rate. The removal of three or more lymph nodes with single-agent mapping tracer might be indicated for breast cancer patients who converted to ycN0 after NAC in the Brazilian health public services, in which radioisotope tracer is not suitable.

Association between spinal alignment and biochemical composition of lumbar intervertebral discs assessed by quantitative magnetic resonance imaging.

BACKGROUND: To evaluate potential associations between spinopelvic parameters and the biochemical composition of lumbar intervertebral discs using quantitative magnetic resonance imaging in asymptomatic young adults. METHODS: Our study group comprised 93 asymptomatic volunteers aged 20-40 years (49 women and 44 men). Lumbar spine T2-weighted images and T2 relaxometry were acquired on a 1.5T MRI scanner. Spinopelvic parameters including sacral slope, pelvic tilt, pelvic incidence, lumbar lordosis, thoracic kyphosis, thoracolumbar alignment, sagittal vertical axis, spinosacral angle, C2 pelvic angle, and T1S1 and L1S1 length were measured on panoramic spine radiographs. RESULTS: Lumbar lordosis decrease correlates with discrete dehydration of nucleus pulposus at all lumbar levels. Also low values of sacral slope, pelvic tilt, pelvic incidence, thoracic kyphosis and spinosacral angle were associated with decrease of T2 relaxation times on annulus fibrosus. CONCLUSIONS: In conclusion, spinopelvic parameters presented a discrete association with lumbar disc composition and water content.

Retrospective whole-genome comparison of Salmonella enterica serovar Enteritidis from foodborne outbreaks in Southern Brazil.

Salmonella enterica serovar Enteritidis is frequently isolated from animal-source foods associated with human salmonellosis outbreaks. This serovar was spread to animal (mainly poultry) farms worldwide in the 1980s, and it is still detected in foods produced in many countries, including Brazil. The present study reports a retrospective genome-wide comparison of S. Enteritidis from foodborne outbreaks in Southern Brazil in the last two decades. Fifty-two S. Enteritidis isolates were obtained from foodborne outbreaks occurring in different cities of the Brazilian southernmost State, Rio Grande do Sul (RS), from 2003 to 2015. Whole-genome sequences (WGS) from these isolates were obtained and comparatively analyzed with 65 additional genomes from NCBI. Phylogenetic and Bayesian analyses were performed to study temporal evolution. Genes related to antibiotic resistance and virulence were also evaluated. The results demonstrated that all S. Enteritidis isolates from Southern Brazil clustered in the global epidemic clade disseminated worldwide originally in the 1980s. Temporal analysis demonstrated that all Brazilian isolates had a tMRCA (time to most recent common ancestor) in 1986 with an effective population size (Ne) increase soon after until 1992, then becoming constant up to now. In Southern Brazil, there was a significant decrease in the spreading of S. Enteritidis in the last decade. In addition, three antibiotic resistance genes were detected in all isolates: aac(6')-Iaa, mdfA, and tet(34). These results demonstrate the high frequency of one only specific S. Enteritidis lineage (global epidemic clade) in foodborne outbreaks from Southern Brazil in the last two decades.

Transcranial ultrasonography as a reliable instrument for the measurement of the cerebral ventricles in rats with experimental hydrocephalus: a pilot study.

PURPOSES: Demonstrate that transcranial ultrasonography (TUS) scanning is viable and useful as a diagnostic method in experimental hydrocephalus, as well as to compare measurements of cerebral and ventricular width obtained from TUS scans of hydrocephalic rats with post-mortem anatomical specimens, aiming for the development of accurate criteria to establish ventricular enlargement and progression of hydrocephalus subsequently. METHODS: Thirty-five male Wistar rats were used. Following hydrocephalus induction, they underwent a transcranial ultrasound scan to measure cerebral and ventricular dimensions, in the fourth and 21 post-induction days. By the end of the experiments, measurements obtained from TUS scans were compared with actual values as seen in the post-mortem specimens of each animal. RESULTS: Ventricular dilation could be clearly visualized in hydrocephalic animals. We performed intraclass correlation coefficient and linear regression analyses that have demonstrated a precise correlation between measurements of TUS scans and post-mortem specimens; we have found a similarity of 0,95 for the cerebral diameter and 0,97 for ventricular width. CONCLUSIONS: Transcranial ultrasonography is a useful and reliable diagnostic tool for experimental hydrocephalus; also, it can be used to assess the progression of ventriculomegaly in animal models of hydrocephalus.

Knowledge, attitudes, and practices of primary health care professionals in coping with COVID-19 in Brazil: a cross-sectional study.

Introduction: The coronavirus disease 2019 (COVID-19) pandemic has posed challenges to healthcare professionals, who needed to quickly adjust impacts on their work processes. Primary health care has become key to fighting the pandemic, as most mild cases seek primary care services as their point of first contact. Objectives: To ascertain the knowledge, attitudes, and practices of health professionals working in primary health care in Brazil early in the COVID-19 pandemic. Methods: Cross-sectional study of a convenience sample. An online questionnaire was made available from May 1 through May 31, 2020. The sole criterion for inclusion was a response rate greater than 30 respondents per Brazilian state. Data were treated descriptively and statistically. Results: Overall, 293 responses were obtained, and the states of Paraná (n = 86), Mato do Grosso do Sul (n = 50) and São Paulo (n = 48) were included in the study. There was a predominance of female respondents (89.1%). Physical therapy (31.6%) and nursing (12.4%) were the most represented occupations. Respondents generally reported moderate knowledge (54.3%) and preparedness (57.6%), with daily information seeking (63.5%) in handbooks and technical guidance publications (89.6%). There was no statistically significant difference between states for the variables knowledge (p = 0.28) and preparedness (p = 0.19). Conclusions: The participating states showed similar, positive results regarding knowledge, attitudes, and practices. Previous experiences seem to generate cumulative knowledge; however, greater readiness in training professionals is needed in emergency situations.

Sentinel lymph node biopsy using single-agent mapping tracer (blue dye) after neoadjuvant chemotherapy in a Brazilian cohort of breast cancer patients. Real world evidence

ABSTRACT Purpose To reduce false-negative rates (FNR) in sentinel lymph node biopsy (SLNB) of clinically positive (cN+) axilla in patients undergoing neoadjuvant chemotherapy (NAC). The removal of three or more lymph nodes with dual-tracer mapping including a radioisotope was used. However, in the Brazilian Unified Health System, the radioisotope tracer is not feasible in some hospitals. We conducted a cross-sectional study to evaluate the detection rate of sentinel lymph node (SLN) in patients who converted from cN+ to ycN0 after NAC using blue dye as a single-agent mapping tracer. Methods During the period of March 2018 to September 2019, 34 patients who underwent NAC with cN+ who converted to ycN0 were enrolled in the study. The SLNB was performed using blue dye as a single-agent mapping followed by axillary lymph node dissection (ALND). Results The detection rate of sentinel lymph node was of 85.3%, being SLNB not possible for five patients (14.7%), due to fibrosis. The mean number of removed SLN was 2.5. Conclusions The use of blue dye as a single-agent mapping tracer demonstrated an acceptable detection rate of 85.3%. Although the FNR was possible to be determined, the small sample size might overestimate this rate. The removal of three or more lymph nodes with single-agent mapping tracer might be indicated for breast cancer patients who converted to ycN0 after NAC in the Brazilian health public services, in which radioisotope tracer is not suitable.