[Detection of Epstein-Barr viral antibodies in patients with acute viral hepatitis]. / Obnaruzhenie antitel k virusu Epshteina-Barr u bol'nykh ostrymi virusnymi gepatitami.

Serum samples obtained from 44 patients with virus A hepatitis, 23 patients with virus B hepatitis, 65 patients with virus non A, non B hepatitis and 100 healthy adults were studied for the presence of Epstein-Barr (EB) virus in the indirect immunofluorescence test. In this work lymphoblastoid cell lines PH3-J-1 and CN37 were used. Among patients with different forms of hepatitis, the statistically significant elevation of the titers of antibodies to EB virus was detected only in the group of patients with virus non A, non B hepatitis, and in 6 cases the etiological role of EB virus was confirmed by serological and hematological methods.

[Formation of immune interferon and blast transformation in stimulated cultures of human lymphocytes]. / Obrazovanie immunogo interferona i blastnaia transformatsiia v stimulirovannykh kul'turakh limfotsitov cheloveka.

Lymphocyte blast transformation (LBT) and interferon (IF) production were studied in 55 phytohemagglutinin- and dry purified tuberculin-stimulated lymphocyte cultures obtained from normal subjects (7 adults and 24 children). A relationship has been disclosed between LBT and interferon production. However, in some of the culture with marked LBT there occurred a defective IF production. On the contrary, with suppressed PHA-induced transformation, IF production was within normal. The role of the interferon production test for estimation of the functional activity of human lymphocytes is discussed.

[Lymphotoxin production by human lymphocytes. III. The action of adenovirus antigen]. / Prosuktsiia limfotoksina limfotsitami cheloveka. Soobshchenie III. Deistvie adenovirusnogo antigena.

In lymphocyte cultures (LC) from 5 immune donors blast transformation occurred as a result of the stimulting action of adenovirus antigen; the supernatants obtained from these cultures produced a cytotoxic effect on L cells, thus suggesting the presence of lymphotoxin (LT). No blast transformation and no LT production were detected in nonstimulated LC used for control, as well as in LC from 9 nonimmune donors. LT production was shown to depend on the time elapsed after recovery from acute adenovirus infection. LT production was found to be most intensive during the first 2 months after recovery.

[Lymphotoxin production by human lymphocytes. I. Study of the supernatants of phytohemagglutinin stimulated human lymphocyte cultures]. / Produktsiia limfotoksina limfotsitami cheloveka. Soobshchenie I. Izuchenie supernatantov kul'tur limfotsitov cheloveka, stimulirovannykh fitogemaggliutininom.

Lymphotoxin was found to be present in supernatants from 22 human lymphocytes cultures stimulated with phytohemagglutinin in a dose of 5 and 10 microgram/ml. The lymphocytes were obtained from the peripheral blood of 6 apparently healthy persons. Lymphotoxin activity was determined by simple and objective method, i.e. by staining the target cells (mouse L-cells) monolayer with crystal violet, with the following determination of optic densities of the L-cells lysates at 570 nm in the spectrophotometer. As revealed, 1 : 5 dilutions of the supernatants from the lymphocyte cultures incubated for 48 hours inhibited the L-cells growth by from 40 to 60%. With further incubation of the cultures (up to 72 and 96 hours) the cytotoxicity of their supernatants for the target cells showed no increase, whereas the blasttransformation index reaches the maximal value by 72nd incubation hour. Supernatants from unstimulated lymphocyte cultures failed to produce any cytotoxic effect on L-cells.