RESUMO
OBJECTIVE: To establish initial processing technology of Corydalis yanhusuo. METHODS: Investigated the effect of the factors such as slice method, dry method, drying temperature on the content of water-extract, ethanol-extract, effective component in Corydalis yanhusuo pieces. Compared the quality with that of the traditional initial processing samples. RESULTS: The best initial process method was: cut fresh Corydalis yanhusuo into 4 - 5 mm thick slices, dry at 70 - 80 degrees C or microwave dry. CONCLUSION: The study provides theoretical base for modifying the initial processing.
Assuntos
Alcaloides/análise , Alcaloides de Berberina/análise , Corydalis/química , Dessecação/métodos , Plantas Medicinais/química , Cromatografia Líquida de Alta Pressão/métodos , Etanol , Micro-Ondas , Extratos Vegetais/análise , Extratos Vegetais/química , Controle de Qualidade , Reprodutibilidade dos Testes , Rizoma/química , Tecnologia Farmacêutica/métodosRESUMO
OBJECTIVE: To choose the best harvest time and initial processing method of Rhizoma Corydalis. METHODS: Taking the content of tetrahydropalmatine and dry rates as major indices, different samples from different harvest time and different processing methods were investigated by HPLC. The C18 column (250 mm x 4.6 mm, 5 microm) was used with mobile phase of methanol-0.1% phosphoric acid solution (pH = 6.0, adjusted with triethylamine) (58: 42). The mobile phase flow rate was 1.0 mL/min. The detection wavelength was 280 nm. RESULTS: The contents of tetrahydropalmatine were similar although the harvest time was different. The dry rate on May 21 was higher than the others. The content of tetrahydropalmatine was the highest by steaming and reached 0.11%. CONCLUSION: The best harvest time for Rhizoma Corydalis is around 10 days after the above-ground plant wilted, and the best initial processing method for Rhizoma Corydalis is steaming.
Assuntos
Alcaloides de Berberina/análise , Corydalis/química , Medicamentos de Ervas Chinesas/química , Plantas Medicinais/química , Tecnologia Farmacêutica/métodos , Alcaloides de Berberina/isolamento & purificação , Cromatografia Líquida de Alta Pressão/métodos , Corydalis/crescimento & desenvolvimento , Medicamentos de Ervas Chinesas/isolamento & purificação , Temperatura Alta , Plantas Medicinais/crescimento & desenvolvimento , Reprodutibilidade dos Testes , Rizoma/química , Estações do Ano , VaporRESUMO
The experiment of cutting propagation of Tripterygium wilfordii Hook. f. was carried out. It showed a better result when stems were instantly planted after being moistened or soaked by 500 - 1000 ppm IBA or NAA. A remarkably higher survival rate was achieved while plasticfilm and shade were accompanied. September and October or rainy June are prime moments for operation. It' s recommendable to choose those newly corking twigs for cuttage.
