Metagenomic analysis reveals the mechanisms of biochar supported nano zero-valent iron in two-phase anaerobic digestion of food waste: microbial community, CAZmey, functional genes and antibiotic resistance genes.

The mechanisms of biochar supported nano zero-valent iron (BC/nZVI) on two-phase anaerobic digestion of food waste were investigated. Results indicated that the performance of both acidogenic phase and methanogenic phase was effectively facilitated. BC/nZVI with the amount of 120 mg/L increased methane production by 32.21%. In addition, BC/nZVI facilitated direct interspecies electron transfer (DIET) between Geobacter and methanogens. Further analysis showed that BC/nZVI increased the abundance of most CAZymes in acidogenic phase. The study also found that BC/nZVI had positive effects on metabolic pathways and related functional genes. The abundances of acdA and ackA in acidogenic phase were increased by 151.75% and 36.26%, respectively, and the abundances of pilA and TorZ associated with DIET were also increased. Furthermore, BC/nZVI mainly removed IMP-12, CAU-1, cmeB, ErmR, MexW, ErmG, Bla2, vgaD, MuxA, and cpxA from this system, and reduced the antibiotic resistance genes for antibiotic inactivation resistance mechanisms.

Effects of Fe3O4 NMs based Fenton-like reactions on biodegradable plastic bags in compost: New insight into plastisphere community succession, co-composting efficiency and free radical in situ aging theory.

Biodegradable plastic bags (BPBs), meant for eco-friendly, often inadequately degrade in compost, leading to microplastic pollution. In this study, the effect of Fenton-like reaction with Fe3O4 nanoparticles (NMs) on the plastisphere microorganisms' evolution and the BPBs' aging mechanism was revealed by co-composting of food waste with BPBs for 40 days. The establishment of the Fenton-like reaction was confirmed, with the addition of Fenton-like reagent treatments resulting in an increase of 57.67% and 37.75% in H2O2 levels during the composting, compared to the control group. Moreover, the structural characterization reveals that increasing oxygen content continuously generates reactive free radicals on the surface, leading to the formation of oxidative cavities. This process results in random chain-breaking, significantly reducing molecular weights by 39.27% and 38.81%, thus showcasing a deep-seated transformation in the plastic's molecular structure. Furthermore, the microbial network suggested that the Fenton-like reaction enriched plastisphere keystone species, thus accelerating the BPBs' aging. Additionally, the Fenton-like reaction improved compost maturity and reduced greenhouse gas emissions. These results reveal the bio-chemical mechanisms of BPBs aging and random chain-breaking by the Fenton-like reaction, under alternating oxidative/anoxic conditions of composting and provide a new insight to resolve the BPBs' pollutions.

Feasibility of anaerobic co-digestion of biodegradable plastics with food waste, investigation of microbial diversity and digestate phytotoxicity.

The effects of biodegradable plastics of different thicknesses (30 and 40 µm) and sizes (20 × 20, 2 × 2, and 1 × 1 mm) on anaerobic digestion of food waste and digestate phytotoxicity were investigated. Methane productions (38 days) for the groups with 20 × 20, 2 × 2, and 1 × 1 mm of 30 µm plastics were 92.46, 138.27, and 259.95 mL/gVSremoval, respectively which are nearly 58 % higher than the control group (58.86 mL/gVSremoval). Methane production in 40 µm plastics groups was lower than in 30 µm groups of equal size. All sizes of 30 µm plastics promoted substrate hydrolysis, acidification, and relative abundance of key hydrolytic bacteria and methanogens. Phytotoxicity tests results showed that seed root elongation was inhibited in groups with 40 µm plastics. In conclusion, 30 µm biodegradable plastics were more suitable for anaerobic digestion with food waste than 40 µm.

Effects of Two Different Proportions of Microbial Formulations on Microbial Communities in Kitchen Waste Composting.

The objective of this research was to investigate the effect of bulking agents on the maturity and gaseous emissions of composting kitchen waste. The composing experiments were carried out by selected core bacterial agents and universal bacterial agents for 20 days. The results demonstrated that the addition of core microbial agents effectively controlled the emission of typical odor-producing compounds. The addition of core and universal bacterial agents drastically reduced NH3 emissions by 94% and 74%, and decreased H2S emissions by 78% and 27%. The application of core microbial agents during composting elevated the peak temperature to 65 °C and in terms of efficient temperature evolution (>55 °C for 8 consecutive days). The organic matter degradation decreased by 65% from the initial values for core microbial agents were added, while for the other treatments the reduction was slight. Adding core microbial agents to kitchen waste produced mature compost with a higher germination index (GI) 112%, while other treatments did not fully mature and had a GI of <70%. Microbial analysis demonstrated that the core microbial agents in composting increased the relative abundances of Weissella, Ignatzschineria, and Bacteroides. Network and redundancy analysis (RDA) revealed that the core microbial agents enhanced the relationship between bacteria and the eight indicators (p < 0.01), thereby improving the bio transformation of compounds during composting. Overall, these results suggest that the careful selection of appropriate inoculation microorganisms is crucial for improved biological transformation and nutrient content composting efficacy of kitchen waste.

Enhancing food waste reduction efficiency and high-value biomass production in Hermetia illucens rearing through bioaugmentation with gut bacterial agent.

The black soldier fly (BSF) rearing technology has been a promising bioconversion method for food waste (FW) disposal. However, when used independently, it currently only achieves low efficiency and biomass transformation rates (BTR). This study screened and identified two strains of gut beneficial bacteria, Bacillus cereus and Bacterium YC-LK-LKJ45. The efficiency of a complex culture formulated by these strains was investigated, focusing on enhancing FW reduction and high-value biomass production during the rearing of BSF larvae. The coculture agent group (G1-10%, with two strains in 1:1 volume ratio at a 10 % dosage) exhibited higher larval yield (627.67 g·kg-1), BTR (47.90 %), FW reduction efficiency (80.67 %), and total protein and fat yield (261.99 g·kg-1and 46.24 g·kg-1) compared to the control and the monoculture agent group (which added a single gut beneficial bacteria agent, either Bacillus cereus or Bacterium YC-LK-LKJ45). The bacterial agent altered the richness and diversity of the gut microbial community of BSF, increasing the relative abundance of beneficial bacteria such as Bacillus, Oceano bacillus, and Akkermansia, while decreasing pathogenic bacteria, such as Acinetobacter and Escherichia-Shigella. Structural equation model quantification revealed that α-diversity (λ = 0.897, p < 0.001) and BTR (λ = 0.747, p < 0.001) are crucial drivers for enhancing high-value biomass during bioaugmentation rearing. This investigation provides a theoretical framework for the effective management of food waste using BSF, enhancing its decomposition and transformation into higher-value biomass.

Suppressive performance of food waste composting with polylactic acid: Emphasis on microbial core metabolism pathways and mechanism.

The aim of this study was to assess the effect of polylactic acid (PLA) on microbial community composition and core metabolism pathways in food waste (FW) composting. The presence of PLA negatively influenced microbial community richness and decreased respectively the abundance of Bacillus, Halocella and Cellvibrio at mesophilic, thermophilic and mature phases. Analysis of microbial metabolism at the gene level help to understand the mechanism in co-composting with FW and PLA. The expression of core functional genes related to lactide metabolism was stimulated by PLA degradation at thermophilic and mature phases. The sum of absolute abundance of functional genes that involved in first and second carbon oxidation of tricarboxylic acid cycle was decreased due to the existence of PLA. The transformation between 2-Oxoglutarate and Succinyl-CoA were interfered in thermophilic phase, which might result in the lower germination index in PLA group (115%) than that in control (186%).

Effect of aeration/micro-aeration on lignocellulosic decomposition, maturity and seedling phytotoxicity during full-scale biogas residues composting.

With the accelerated construction of biogas plants, the amount of biogas residues are expanding. Composting has been widely implemented to deal with biogas residues. Aeration regulation is the main factor affecting the post-composting treatment of biogas residues as high-quality fertilizer or soil amendment. Therefore, this study aimed to investigate the impact of different aeration regulations on full-scale biogas residues compost maturity by controlling oxygen concentration under micro-aeration and aeration conditions. Results showed that micro-aerobic extended the thermophilic stage of 17 days at above 55 ℃ and facilitated the mineralization process of organic nitrogen into nitrate nitrogen to retain higher N nutrition levels compared to aerobic treatment. For biogas residues with high moisture, aeration should be regulated at different full-scale composting stages. Total organic carbon (TOC), NH4+-N, NO3--N, total potassium (TK), total phosphorus (TP) and the germination index (GI) could be used to evaluate stabilization, fertilizer efficiency and phytotoxicity of compost with frequent monitoring times. However, seedling growth trials were still necessary in full-scale composting plants when changing of composting process or biogas residues feedstock.

Effects of biochar supported nano zero-valent iron with different carbon/iron ratios on two-phase anaerobic digestion of food waste.

The promotion effects of biochar supported nano zero-valent iron (BC/nZVI) with different carbon/iron ratios on two-phase anaerobic digestion (AD) of food waste (FW) were studied. Results suggested that when the carbon/iron ratio was 3:1 AD system showed the best performance, with the concentration of volatile fatty acids (VFAs) in acidogenic phase (AP) and the cumulative methane production in methanogenic phase (MP) increased by 31.4% and 24.8%, respectively. Metagenomic analysis demonstrated that BC/nZVI increased the relative abundance of Defluviitoga in AP, and promoted the growth of Methanothrix in MP. Metabolic pathway analysis in AP indicated that BC/nZVI mainly promoted the abundances of acetate kinase and butyrate kinase to enhance acid production. Methane metabolism pathway analysis in MP revealed that BC/nZVI increased methane production by promoting the module of M00357 and activating related enzymes. The results of this sutdy showed that BC/nZVI promoted AD of FW mainly through acetoclastic methanogenic pathway.

Electronic nose application for detecting different odorants in source water: Possibility and scenario.

The problem of taste and odor (T&O) in drinking water is a widespread societal concern and highlights substantial challenges related to the detection and evaluation of odor in water. In this study, the portable electronic nose PEN3, which is equipped with ten different heated metal sensors, was applied to analyze its applicability, feasibility and application scenarios for the detection of typical odorants, such as 2-methylisobornel (2-MIB), geosmin (GSM), ß-cyclocitral, ß-ionone, and other T&O compounds in source water, while avoiding uncertainties and instability related to manual inspection. All the T&O compounds could be effectively differentiated by principal component analysis (PCA). Linear discriminant analysis (LDA) showed that the odors varied greatly between different samples and could be effectively distinguished. As the odorant concentration increased, the sensor response intensity of the primary identification sensors R6 and R8 increased with a significant positive correlation. For Microcystis aeruginosa, an algae that produces odorants, PCA could distinguish the odors of algae at a series of densities at different concentrations. The responses of R10 showed a significant increase with increasing algae density, implying the production of more aliphatic hydrocarbons and other odor compounds. The results indicated that the electronic nose could provide a promising alternative to traditional unstable and complex detection methods for the detection of odorous substances in surface water and early warning of odor events. This study aimed to provide technical support for rapid monitoring and early warning of odorants in source water management.

Appraising co-composting efficiency of biodegradable plastic bags and food wastes: Assessment microplastics morphology, greenhouse gas emissions, and changes in microbial community.

Biodegradable plastic bags (BPBs) to collect food waste and microplastics (MPs) produced from their biodegradation have received considerable scientific attention recently. Therefore, the current study was carried out to assess the co-composting efficiency of biodegradable plastic bags (polylactic acid (PLA) + polybutylene terephthalate (PBAT) + ST20 and PLA + PBAT+MD25) and food waste. The variations in greenhouse gas (GHG) emissions, microbial community and compost fertility were likewise assessed. Compared with the control, PLA + PBAT+ST20 and PLA + PBAT+MD25 both accelerated organic matter degradation and increased temperature. Moreover, PLA + PBAT+ST20 aggravated CH4 and CO2 emissions by 12.10 % and 11.01 %, respectively. PLA + PBAT+MD25 decreased CH4 and CO2 emissions by 5.50 % and 9.12 %, respectively. Meanwhile, compared with PLA + PBAT+ST20, the combined effect of plasticizer and inorganic additive in PLA + PBAT+MD25, reduced the NO3--N contents, seed germination index (GI) and compost maturity. Furthermore, adding BPBs changed the richness and diversity of the bacterial community (Firmicutes, Proteobacteria and Bacteroidetes). Likewise, redundancy analysis (RDA) showed that the co-compost system of BPBs and food waste accelerated significantly bacterial community succession from Firmicutes and Bacteroidetes at the initial stage to Proteobacteria and Actinobacteria at the mature stage, increased co-compost temperature to over 64 °C and extended thermophilic composting phase, and promoted the degradation of MPs. Additionally, according to structural equation model quantification results, the inorganic additive of PLA + PBAT+MD25 had more serious toxicity to microorganisms and had significantly adverse effects on GI through CO2-C (λ = -0.415, p < 0.05) and NO3--N (λ = -0.558, p < 0.001), thus reduced compost fertility and quality. The results also indicated that the BPBs with ST20 as an additive could be more suitable for industrial composting than the BPBs with MD25 as an additive. This study provided a vital basis for understanding the potential environmental and human health risks of the MPs' generated by the degradation of BPBs in compost.

The greenhouse gas emission potential and phytotoxicity of biogas slurry in static storage under different temperatures.

With the booming development of biogas industry to treat organic waste in China, the by-product of biogas slurry was accompanied with a huge amount. Static storage process of biogas slurry was normally operated under different seasons before application to land which would cause nutrition decomposition and greenhouse gas emission. Thus, the aim of this study was to investigate the nutrition decomposition, greenhouse gas emission (CH4 and N2O), and phytotoxicity of biogas slurry under different static temperatures, furthermore to illuminate the network among them and functional microorganism. According to the results, higher temperature at 30 °C contributed to fast and complete degradation of COD. In addition, more quantity of NH4+ conversion and NO3- formation appeared at 30 °C. These factors resulted in relatively less crop toxicity together. CH4 was the dominant greenhouse gas emission than N2O and was highest in 30 °C treatment with total emission of 273.7 L/(m3·d) and greenhouse gas emission of 20.01 kg CO2e (carbon dioxide equivalent). Lower temperature was conductive to N reservation and reduction of greenhouse gas emission, but making against with stabilization of organic matter and crop safety. At the same dilution times (≤3) of biogas slurry with deionized water, higher temperature at 30 °C could reduce 30 days of storage time, but 10 °C was still unsafe for crop. Structural equation model was further illustrated the positive effect of temperature on NO3-, CH4, GI, and N2O and negative on COD and NH4+. These results could help to monitor the environmental risk, evaluate the maturity, guide the irrigation scheme, and regulate the static storage condition of biogas slurry under different seasons.

Untargeted metabolomics elucidated biosynthesis of polyhydroxyalkanoate by mixed microbial cultures from waste activated sludge under different pH values.

Waste activated sludge has been frequently used as mixed substrate to produce polyhydroxyalkanoate (PHA). However, insufficient research on microbial metabolism has led to difficulties in regulating PHA accumulation in mixed microbial cultures (MMCs). To explore the variation of functional genes during domestication and the effect of different pH conditions on metabolic pathways during PHA accumulation, MMCs were domesticated by adding acetate and propionate with aerobic dynamic feeding strategy for 60 days. As the domestication progressed, the microbial community diversity declined and PHA-producing bacteria, Brevundimonas, Dechloromonas and Hyphomonas, were enriched. Through bacterial function prediction by PICRUSt the gene rpoE involved in starvation resistance of bacteria was enriched after the domestication. The pH value of 8.5 was the best condition for PHA accumulation in MMCs, under which a maximum PHA content reached 23.50% and hydroxybutyric (HB)/hydroxyvaleric (HV) reached 2.22. Untargeted metabolomics analysis exhibited that pH conditions of 7 and 8.5 could promote the up-regulation of significant differential metabolites, while higher alkaline conditions caused the inhibition of metabolic activity. Functional annotation showed that pH condition of 8.5 significantly affected Pyrimidine metabolism, resulting in an increase in PHA production. Regarding the pathways of PHA biosynthesis, acetoacetate was found to be significant in the metabolism of hydroxybutyric, and the alkaline condition could restrain the conversion from hydroxybutyric (HB) to the acetoacetate to protect PHB accumulation in MMCs compared with neutral condition. Taken together, the present results can advance the fundamental understanding of metabolic function in PHA accumulation under different pH conditions.

New insights of anaerobic performance, antibiotic resistance gene removal, microbial community structure: applying graphite-based materials in wet anaerobic digestion.

The addition of carbonaceous materials into anaerobic digestion (AD) has gained widespread attention due to their significant effects on anaerobic performance and antibiotic resistance gene (ARG) removal. This study selected graphite, graphene, and graphene oxide (GO) as additives to investigate variations in AD performance, ARG removal, microbial community diversity and structure in wet AD systems. The results indicated that the addition of graphite-based materials in wet AD systems could increase degradation of solid organic matters by 0.91%-3.41% and utilization of soluble organic fractions by 10.43%-13.67%, but could not stimulate methane production. After the addition of graphite and graphene, ARG removal rates were effectively increased to 90.85% and 94.22%, respectively. However, the total ARG removal rate was reduced to 77.46% with the addition of GO. In addition, the microbial diversity in the wet AD process was enhanced with the addition of GO only, graphite and graphene led to a reduction in it. As for bacterial community, graphite and graphene increased the abundance of Thermotogae from 43.43% to 57.42% and 58.74%, while GO increased the abundance of Firmicute from 49.90% to 56.27%. For the archaeal community, the proportion of hydrogenotrophic methanogens was improved when adding each graphite-based material; however, only GO increased Methanosaeta that was acetoclastic methanogens. Finally, methanogens were found as the ARG host, and ARGs that belong to the same subtype might exist in the same host bacteria.

Metagenomics insight into bioaugmentation mechanism of Propionibacterium acidipropionici during anaerobic acidification of kitchen waste.

In the present study, a biochemical strategy for improving propionic acid production from kitchen waste acidification by bioaugmentation with Propionibacterium acidipropionici (P. acidipropionici) was investigated. When the inoculum of P. acidipropionici was 30% (w/w) of the seeding sludge, the propionic acid production increased by 79.57%. Further, bioaugmentation improved the relative abundance of Firmicute and Actinobacteria. The results of metagenomic analysis further reveal that the ATP-binding cassette (ABC) transporters and all related pathways of Propanoate metabolism (ko00640) were enriched when P. acidipropionici was added. For Propanoate metabolism, most functional genes involved in the conversion from Glycolysis / Gluconeogenesis (ko00010) to Propanoyl-CoA and conversion from Propanoyl-CoA to propionic acid were enhanced after bioaugmentation with P. acidipropionici, thereby promoting propionic acid production. As such, bioaugmentation with P. acidipropionici was effective in the anaerobic acidification of kitchen waste for propionic acid production.

Food Waste Management Employing UV-Induced Black Soldier Flies: Metabolomic Analysis of Bioactive Components, Antioxidant Properties, and Antibacterial Potential.

Food waste, as a major part of municipal solid waste, has been increasingly generated worldwide. Efficient and feasible utilization of this waste material for biomanufacturing is crucial to improving economic and environmental sustainability. In the present study, black soldier flies (BSF) larvae were used as carriers to treat and upcycle food waste. Larvae of the BSF were incubated with UV light for 10, 20, and 30 min at a wavelength of 257.3 nm and an intensity of 8 W. The food waste utilization efficiency, antioxidant assays, antibacterial activity, and bioactive metabolites without and with UV treatment were determined and compared. Results showed that the BSF larvae feed utilization rate was around 75.6%, 77.7%, and 71.2% after UV treatment for 10, 20, and 30 min respectively, contrasting with the non-UV induced group (73.7%). In addition, it was perceived that the UV exposure enhanced antioxidant and antimicrobial properties of BSF extracts, and the maximum values were observed after 20 min UV induction time. Moreover, UV-induced BSF extracts showed an improved metabolic profile than the control group, with a change in the amino acids, peptides, organic acids, lipids, organic oxides, and other derivatives. This change in metabolomics profile boosted environmental signaling, degradation of starch, amino acids, sugars, and peptide metabolism. It was concluded that the bioconversion of food wastes using UV-induced BSF larvae can enhance the generation of a variety of functional proteins and bioactive compounds with potent antioxidant and antimicrobial activity. However, more studies are required to exploit the efficiency of UV treatment in improving BSF's potential for upcycling of food wastes.

Untargeted Metabolomics Profiling of Bioactive Compounds under Varying Digestate Storage Conditions: Assessment of Antioxidant and Antifungal Activity.

The rapid development of biogas plants in China has generated large quantities of digestate. The disparity between the continuity of biogas plant operation and the seasonality of digestate utilization has led to the need to store digestate. Therefore, untargeted profiling of bioactive compounds in the digestate stored under aerobic and anaerobic conditions was performed. The antioxidant and antifungal activity of digestate stored under varying conditions was likewise assessed. The results delineated that digestate storage under varying conditions brought about the degradation of organic acids, alkenes, aldehydes, alcohols, ketones, ethers, amino acids and their derivatives, and esters, leading to the stabilization of digestate components. Together, these new data revealed that digestate storage for up to 20 days under aerobic conditions promotes glycine, serine, and threonine degradation pathways and enhances biotin and vitamins production. In contrast, anaerobic storage enhances the taurine and hypotaurine metabolic pathways and increases the derivation of antimicrobial substances, such as indole alkaloids. Moreover, digestate storage under anaerobic conditions promotes antioxidant and antifungal activity more than storage under aerobic conditions. These findings can contribute to the future development of high-value agricultural products from digestate and the sustainability of biogas plants. Further studies are required for the untargeted metabolomic of digestate under storage to explore the underlying mechanisms of promoting disease resistance by the digestate upon land application.

Food waste anaerobic biogas slurry as fertilizer: Potential salinization on different soil layer and effect on rhizobacteria community.

Biogas slurry(BS) from food waste anaerobic fermentation coexisted a lot of salinity that could damage soil and crops health. So, this study was to explore the effect of the application of biogas slurry on soil salinization in 1 âˆ¼ 4 cm, 4-6 cm and 6 âˆ¼ 8 cm soil layers every 10 days, Chinese cabbage growth and rhizobacteria. The results indicated that ≤ 10% concentration of biogas slurry was uninjurious for soil and plant, the dry weight growth rate was 73.7% compared with CK, long term application should be further evaluated the potential risk of salinity on underground water and human health. As for high concentration of biogas slurry ≥ 10% concentration of biogas slurry could inhibit the seed germination and root elongation, and the germination percentage was declined from 87.6% to 2.4%, but 50% and 100% concentration of biogas slurry showed a promotion of crop growth because of sufficient nutrition. However, the potential accumulation of salinity could be seen in high concentration of biogas slurry for long term application especially in top1-4 cm soil. Correlation analysis showed that Cl- was the main factor resulting high EC in all soil layers. 16S rRNA sequencing showed that UCG-004, Ketobacter, Sphingopyxis and RB41 could be regard as the indicators for determining the potential jeopardize on soil environmental by high salinity from biogas slurry.

Probing the antioxidant activity of functional proteins and bioactive peptides in Hermetia illucens larvae fed with food wastes.

Food waste is becoming more prevalent, and managing it is one of the most important issues in terms of food safety. In this study, functional proteins and bioactive peptides produced from the enzymatic digestion of black soldier fly (Hermetia illucens L., BSF) fed with food wastes were characterized and quantified using proteomics-based analysis. The results revealed approximately 78 peptides and 57 proteins, including 40S ribosomal protein S4, 60S ribosomal protein L8, ATP synthase subunit alpha, ribosomal protein S3, Histone H2A, NADP-glutamate dehydrogenase, Fumarate hydratase, RNA helicase, Chitin binding Peritrophin-A, Lectin C-type protein, etc. were found in BSF. Furthermore, functional analysis of the proteins revealed that the 60S ribosomal protein L5 (RpL5) in BSF interacted with a variety of ribosomal proteins and played a key role in the glycolytic process (AT14039p). Higher antioxidant activity was found in peptide sequences such as GYGFGGGAGCLSMDTGAHLNR, VVPSANRAMVGIVAGGGRIDKPILK, AGLQFPVGR, GFKDQIQDVFK, and GFKDQIQDVFK. It was concluded that the bioconversion of food wastes by BSF brought about the generation of a variety of functional proteins and bioactive peptides with strong antioxidant activity. However, more studies are required to exploit BSF's potential in the value addition of food wastes.

Performance and metagenomics analysis of anaerobic digestion of food waste with adding biochar supported nano zero-valent iron under mesophilic and thermophilic condition.

A large amount of food waste (FW) brings environmental pollution and sanitation problems. Anaerobic digestion (AD) is an effective technology to treat FW and generate biogas energy. This study investigated the effect of biochar supported nano zero-valent iron (BC-nZVI) on AD performance of FW. Results showed that the cumulative methane yield (CMY) increased by 21.52%-54.90% and the lag time decreased significantly with BC-nZVI. Under mesophilic and thermophilic condition, the peak of CMY was achieved at 178.82 ± 5.27 mL/g VS and 193.01 ± 6.81 mL/g VS with 5 g/L BC-nZVI, respectively. Besides, BC-nZVI stimulated hydrolysis process and reduced the inhibition of NH4+-N and volatile fatty acids accumulation, and it could improve the system stability. Structural equation model analysis indicated that digestion time, BC-nZVI, NH4+-N, temperature and total volatile fatty acid had significant effects on CMY, explaining 92.20% of its total variation. The metagenomic analysis of key microorganisms and related metabolism pathways involved in AD system was further investigated. The results suggested that BC-nZVI contributed to strengthen methanogenesis through enriching the various predominant methanogenic pathways and activating most enzymes related to methane metabolism. BC-nZVI could improve the AD system function and provided a better AD performance by shifting the microbial communities and altering functional genes. This study provided a theoretical basis for BC-nZVI applications and improvements in AD process of FW.

The formation and control of ozonation by-products during drinking water advanced treatment in a pilot-scale study.

Ozone has been widely applied during drinking water treatment for organic pollutants removal, however, the ozonation by-products such as bromate, aldehydes, and carboxylic acids could be produced. To investigate the ozonation by-products formation and control efficiency, a continuous pilot-scale system was established including conventional treatment and ozone-biological activated carbon (O3-BAC) treatment processes in a waterworks that receives bromide-bearing source water from the Yellow River in northern China. It was found that ozonation could reduce the threshold odour number and the haloacetic acids formation potential effectively. As 1.8-2.6 mg/L of ozone was applied, the bromate concentration would exceed the standard of 10 µg/L. Simultaneously, ozonation led to a large number of formaldehyde, methylglyoxal and other aldehydes. At the ozone dosage of 3.0 mg/L, the total concentration of aldehydes reached the highest of 45.4 µg/L. Ammonia and hydrogen peroxide (H2O2) addition could inhibit the formation of bromate effectively. The application of H2O2 induced an increase in aldehydes concentration reaching a maximum concentration of 57.6 µg/L. During the BAC treatment, the removal rate of aldehydes would reach 19%-41%. The addition of H2O2 inhibited the formation of bromate but increased the production of aldehydes, thus it is important to search a balance point for controlling bromate and aldehydes simultaneously. The H2O2/O3 (g/g) of 1.0 could be possible to control both bromate and aldehydes concentrations within the standards.