Polarity-Driven Fluorescence Monitoring of Lipid Droplet Dynamics in Dry Eye Disease.

The emergence of lipid droplets (LDs) has been recognized as cellular markers of ocular surface hyperosmosis, which is recognized as a fundamental mechanism driving dry eye disease (DED), while their dynamics during DED progression and therapy remains unlocked. For this purpose, an LD-specific fluorescent probe P1 is presented in this work that exhibits highly selective and sensitive emission enhancement in response to a decreased ambient polarity (Δf) from 0.209 to 0.021. The hydrophobic nature of P1 enables specific staining of LDs, facilitating visualization of changes in polarity within these cellular structures. Utilizing P1, we observe a decrease in polarity accompanied by an increase in the size and number of LDs in hyperosmotic human corneal epithelial cells (HCECs). Furthermore, interplays between LDs and cellular organelles such as mitochondria and the Golgi apparatus are visualized, suggesting the underlying pathogenesis in DED. Notably, the variations of LDs are observed after the inhibition of ferroptosis or activation of autophagy in hyperosmotic HCECs, implying the great potential of LDs as indicators for the design and efficacy evaluation of DED drugs regarding ferroptosis or autophagy as targets. Finally, LDs are confirmed to be overproduced in corneal tissues from DED mice, and the application of clinical eye drops effectively impedes these changes. This detailed exploration underscores the significant roles of LDs as an indicator for the deep insight into DED advancement and therapy.

Comparison of deep learning-assisted blinking analysis system and Lipiview interferometer in dry eye patients: a cross-sectional study.

BACKGROUND: Abnormal blinking pattern is associated with ocular surface diseases. However, blink is difficult to analyze due to the rapid movement of eyelids. Deep learning machine (DLM) has been proposed as an optional tool for blinking analysis, but its clinical practicability still needs to be proven. Therefore, the study aims to compare the DLM-assisted Keratograph 5M (K5M) as a novel method with the currently available Lipiview in the clinic and assess whether blinking parameters can be applied in the diagnosis of dry eye disease (DED). METHODS: Thirty-five DED participants and 35 normal subjects were recruited in this cross-sectional study. DED questionnaire and ocular surface signs were evaluated. Blinking parameters including number of blinks, number of incomplete blinking (IB), and IB rate were collected from the blinking videos recorded by the K5M and Lipiview. Blinking parameters were individually collected from the DLM analyzed K5M videos and Lipiview generated results. The agreement and consistency of blinking parameters were compared between the two devices. The association of blinking parameters to DED symptoms and signs were evaluated via heatmap. RESULTS: In total, 140 eyes of 70 participants were included in this study. Lipiview presented a higher number of IB and IB rate than those from DLM-assisted K5M (P ≤ 0.006). DLM-assisted K5M captured significant differences in number of blinks, number of IB and IB rate between DED and normal subjects (P ≤ 0.035). In all three parameters, DLM-assisted K5M also showed a better consistency in repeated measurements than Lipiview with higher intraclass correlation coefficients (number of blinks: 0.841 versus 0.665; number of IB: 0.750 versus 0.564; IB rate: 0.633 versus 0.589). More correlations between blinking parameters and DED symptoms and signs were found by DLM-assisted K5M. Moreover, the receiver operating characteristic analysis showed the number of IB from K5M exhibiting the highest area under curve of 0.773. CONCLUSIONS: DLM-assisted K5M is a useful tool to analyze blinking videos and detect abnormal blinking patterns, especially in distinguishing DED patients from normal subjects. Large sample investigations are therefore warranted to assess its clinical utility before implementation.

Fluorescence Imaging of Diabetic Cataract-Associated Lipid Droplets in Living Cells and Patient-Derived Tissues.

Diabetic cataract (DC) surgery carries risks such as slow wound healing, macular edema, and progression of retinopathy and is faced with a deficiency of effective drugs. In this context, we proposed a protocol to evaluate the drug's efficacy using lipid droplets (LDs) as the marker. For this purpose, a fluorescent probe PTZ-LD for LDs detection is developed based on the phenothiazine unit. The probe displays polarity-dependent emission variations, i.e., lower polarity leading to stronger intensity. Especially, the probe exhibits photostability superior to that of Nile Red, a commercial LDs staining dye. Using the probe, the formation of LDs in DC-modeled human lens epithelial (HLE) cells is validated, and the interplay of LDs-LDs and LDs-others are investigated. Unexpectedly, lipid transfer between LDs is visualized. Moreover, the therapeutic efficacy of various drugs in DC-modeled HLE cells is assessed. Ultimately, more LDs were found in lens epithelial tissues from DC patients than in cataract tissues for the first time. We anticipate that this work can attract more attention to the important roles of LDs during DC progression.

Elliptical deep anterior lamellar keratoplasty in severe Acanthamoeba keratitis.

Acanthamoeba keratitis (AK) is an intractable infection of the cornea. Penetrating keratoplasty is widely used for the management of severe AK but suffers from complications like graft rejection, endophthalmitis, and glaucoma. Herein, we aimed to describe the surgical technique and the results of elliptical deep anterior lamellar keratoplasty (eDALK) for the management of severe AK. In this retrospective case series, records of consecutive patients with AK poorly responsive to medical treatment who underwent eDALK from January 2012 to May 2020 were reviewed. The largest diameter of infiltration was ≥8 mm and did not involve the endothelium. The recipient bed was made by an elliptical trephine, and big bubble or wet-peeling technique was performed. Postoperative best spectacle-corrected visual acuity, endothelial cell density, corneal topographic data, and complications were evaluated. Thirteen eyes of thirteen patients (eight men and five women, 45.54 ± 11.78 years old) were included in this study. The mean follow-up interval was 21.31 ± 19.59 months (range, 12-82 months). At the last follow-up, the mean best spectacle-corrected visual acuity was 0.35 ± 0.27 logarithm of the minimum angle of resolution. The mean refractive and topographic astigmatism were - 3.21 ± 1.77 and 3.08 ± 1.14 D, respectively. Intraoperative perforation was encountered in one case and double anterior chambers occurred in two cases. One graft developed stromal rejection and one eye developed amoebic recurrence. eDALK can serve as the first-line surgical management of severe AK poorly responsive to medical treatment.

SIRT1 Inhibits High Glucose-Induced TXNIP/NLRP3 Inflammasome Activation and Cataract Formation.

Purpose: To determine whether SIRT1 regulates high glucose (HG)-induced inflammation and cataract formation through modulating TXNIP/NLRP3 inflammasome activation in human lens epithelial cells (HLECs) and rat lenses. Methods: HG stress from 25 to 150 mM was imposed on HLECs, with treatments using small interfering RNAs (siRNAs) targeting NLRP3, TXNIP, and SIRT1, as well as a lentiviral vector (LV) for SIRT1. Rat lenses were cultivated with HG media, with or without the addition of NLRP3 inhibitor MCC950 or SIRT1 agonist SRT1720. High mannitol groups were applied as the osmotic controls. Real-time PCR, Western blots, and immunofluorescent staining evaluated the mRNA and protein levels of SIRT1, TXNIP, NLRP3, ASC, and IL-1ß. Reactive oxygen species (ROS) generation, cell viability, and death were also assessed. Results: HG stress induced a decline in SIRT1 expression and caused TXNIP/NLRP3 inflammasome activation in a concentration-dependent manner in HLECs, which was not observed in the high mannitol-treated groups. Knocking down NLRP3 or TXNIP inhibited NLRP3 inflammasome-induced IL-1ß p17 secretion under HG stress. Transfections of si-SIRT1 and LV-SIRT1 exerted inverse effects on NLRP3 inflammasome activation, suggesting that SIRT1 acts as an upstream regulator of TXNIP/NLRP3 activity. HG stress induced lens opacity and cataract formation in cultivated rat lenses, which was prevented by MCC950 or SRT1720 treatment, with concomitant reductions in ROS production and TXNIP/NLRP3/IL-1ß expression levels. Conclusions: The TXNIP/NLRP3 inflammasome pathway promotes HG-induced inflammation and HLEC pyroptosis, which is negatively regulated by SIRT1. This suggests viable strategies for treating diabetic cataract.

Efficiently suppress of ferroptosis using deferoxamine nanoparticles as a new method for retinal ganglion cell protection after traumatic optic neuropathy.

Traumatic optic neuropathy (TON) is the major contributor to optic nerve damage, where the retinal ganglion cells (RGCs) are substantially lost. However, the underlying pathological mechanisms for these conditions remain largely elusive. Present work conducted a study on TON rat model, where the iron-dependent cyclooxygenase-2 (COX-2) overexpression and lipid peroxidation were observed in RGCs, suggesting ferroptosis, an iron-dependent non-apoptotic cell death, is involved in TON-induced death of RGCs. Hence, the newly formulated hyaluronic acid (HA)-based deferoxamine (DFO) nanoparticles (DFO-NPs) were intravitreally administrated in the rat model. It was hypothesized that the effective delivery of DFO, iron chelator, to the RGCs might rescue RGC ferroptosis from TON-induced injury. Also, since DFO is poor in bioavailability and of very short half-life in vivo, its safe and efficient intravitreal delivery is critical. Therefore, DFO-NPs were prepared by chemical grafting DFO onto HA molecules, and then crosslinking them in microemulsion bubbles for nanoparticles formulation. The nanoparticles were highly accumulated around the ganglionic cells and DFO uptake was increased in RGCs, accompanied by the significantly inhibited the overexpression of COX-2 and inactivation of glutathione peroxidase 4 (GPX4). These results indicate that DFO-NPs acted as an effective ferroptosis inhibitor, for the prevention of TON-induced RGC death. The current study provides new insights into the underlying mechanism of TON-induced RGC death, which may help to explore a novel strategy for the treatment of TON.

Impact of cone base diameter on outcomes of deep anterior lamellar keratoplasty in keratoconus.

PURPOSE: To evaluate the outcomes of deep anterior lamellar keratoplasty (DALK) in treating keratoconus in relation to cone base diameter (CBD). METHODS: A retrospective study. Sixty-one eyes of 49 keratoconus patients who underwent DALK between 2009 and 2018 were enrolled. Preoperative and postoperative uncorrected visual acuity (UCVA), best-corrected visual acuity (BCVA), spherical equivalent, and astigmatism were measured. Scheimpflug tomography (Pentacam) was used to measure the cone base area (CBA) and CBD using MATLAB software. RESULTS: The mean age of the patients was 20.8 ± 6.1 years old, and the mean follow-up time was 27.3 ± 15.2 months. Mean UCVA improved from 1.23 ± 0.48 to 0.57 ± 0.27 (LogMAR, 95% CI [0.52, 0.80]; P < 0.001), whereas mean BCVA improved from 0.98 ± 0.55 to 0.18 ± 0.13 (95% CI [0.66, 0.94]; P < 0.001). The mean spherical equivalent decreased by 4.53 ± 5.65 D (95% CI [- 6.25, - 2.82]; P < 0.001), with little change in astigmatism (95% CI [- 1.39, 0.64]; P = 0.457). The postoperative BCVA in the patients with CBD < 5.07 mm and corneal curvature ≥ 55D was significantly better than those whose CBD ≥ 5.07 mm (0.14 ± 0.09 vs 0.25 ± 0.15, P = 0.001). The follow-up time was negatively correlated with the BCVA (P = 0.004). CONCLUSIONS: In this study, outcomes of DALK in keratoconus were related to CBD and corneal curvature. Patients with large CBD (≥ 5.07 mm) where the corneal curvature ≥ 55D are more likely to have poor visual outcomes after DALK.

MiR-223 inhibits hyperosmolarity-induced inflammation through downregulating NLRP3 activation in human corneal epithelial cells and dry eye patients.

We previously showed that increases in reactive oxygen species (ROS) generation upregulate NLRP3 inflammasome and inflammation through increases in both caspase-1 activity and rises in IL-1ß expression levels in animal models of dry eye (DE). As changes in microRNA (miRNAs) expression levels can modulate inflammasome function, we determine here if there is a relationship in DE between changes in miR-223 expression levels and NLRP3 activation induced in an intelligent controlled environmental system (ICES) in mice. In parallel, ROS, miR-223 and NLRP3 expression levels were assessed in conjunctival impression cytology and tear fluid samples obtained from DE patients and normal subjects. MiR-223 expression levels were modulated by transfection of either a mimic or its negative control (NC) in a human corneal epithelial cell line (HCECs) exposed to a 500 mOsm hyperosmotic medium for 4 h. The dual-luciferase reporter assay confirmed that miR-223 controls NLRP3 gene expression readout through directly interacting with the 3' UTR of its mRNA. Hyperosmolarity-induced NLRP3 activation was confirmed based on recruitment and colocalization of NLRP3 with ASC as well as increases in IL-1ß expression. The miR-223 expression level decreased by 55% in the conjunctiva and cornea of the murine DE model from the level in the control group (P ≤ 0.047), while NLRP3 protein expression rose by 30% (P ≤ 0.017). In DE patients, miR-223 expression decreased in conjunctival impression cytology samples (P = 0.002), whereas IL-1ß tear content rose significantly (P < 0.001).The relevance of this decline was confirmed by showing that exposure to a 500 mOsm stress decreased the miR-223 expression level whereas ROS generation as well as the NLRP3, and IL-1ß expression levels rose in HCECs (P ≤ 0.037). In contrast, miR-223 mimic transfection reduced the NLRP3 protein expression level by 30% (P = 0.037), whereas both ROS generation and IL-1ß secretion rose compared to their corresponding levels in the control group (P ≤ 0.043). Thus, miR-223 negatively regulates NLRP3 inflammasome activity via suppressing NLRP3 translation in DE. This inverse regulation between miR-223 and NLRP3 expression levels suggests that selective upregulation of miR-223 expression may be a novel option to suppress chronic inflammation in DE.

Observation of HOCl generation associated with diabetic cataract using a highly sensitive fluorescent probe.

Diagnosis of diabetic cataract (DC) in the early stage is of great significance for drug intervention and surgery circumvention for DC patients. However, the lack of reliable imaging tools greatly limits the diagnosis of early DC. In this context, a fluorescent probe BBPy for hypochlorous acid (HOCl) is presented based on the oxidation of phenothiazine. The probe displays apparent emission enhancement at 562 nm toward HOCl with high selectivity, superb sensitivity (detection limit: 12.6 nM), and rapid response (within seconds). Using the probe, the HOCl generation in diabetic human lens epithelial cells was monitored, as well as the HOCl down-regulation during antioxidant treatment. Therefore, it is proposed that HOCl can be a promising biomarker for DC and fluorescence imaging technique can be regarded as a candidate tool for DC diagnosis.

iUMRG: multi-layered network-guided propagation modeling for the inference of susceptibility genes and potential drugs against uveal melanoma.

Uveal melanoma (UM) is the most common primary malignant intraocular tumor. The use of precision medicine for UM to enable personalized diagnosis, prognosis, and treatment require the development of computer-aided strategies and predictive tools that can identify novel high-confidence susceptibility genes (HSGs) and potential therapeutic drugs. In the present study, a computational framework via propagation modeling on integrated multi-layered molecular networks (abbreviated as iUMRG) was proposed for the systematic inference of HSGs in UM. Under the leave-one-out cross-validation experiments, the iUMRG achieved superior predictive performance and yielded a higher area under the receiver operating characteristic curve value (0.8825) for experimentally verified SGs. In addition, using the experimentally verified SGs as seeds, genome-wide screening was performed to detect candidate HSGs using the iUMRG. Multi-perspective validation analysis indicated that most of the top 50 candidate HSGs were indeed markedly associated with UM carcinogenesis, progression, and outcome. Finally, drug repositioning experiments performed on the HSGs revealed 17 potential targets and 10 potential drugs, of which six have been approved for UM treatment. In conclusion, the proposed iUMRG is an effective supplementary tool in UM precision medicine, which may assist the development of new medical therapies and discover new SGs.

Impact of Incomplete Blinking Analyzed Using a Deep Learning Model With the Keratograph 5M in Dry Eye Disease.

Purpose: To establish a deep learning model (DLM) for blink analysis, and investigate whether blink video frame sampling rate influences the accuracy of analysis. Methods: This case-controlled study recruited 50 dry eye disease (DED) participants and 50 normal subjects. Blink videos recorded by a Keratograph 5M, symptom questionnaires, and ocular surface assessments were collected. After processing the blink images as datasets, further training and evaluation of DLM was performed. Blink videos of 30 frames per second (FPS) under white light, eight FPS extracted from white light videos, and eight FPS under infrared light were processed by DLM to generate blink profiles, allowing comparison of blink parameters, and their association with DED symptoms and signs. Results: The blink parameters based on 30 FPS video presented higher sensitivity and accuracy than those based on eight FPS. The average relative interpalpebral height (IPH), the frequency and proportion of incomplete blinking (IB) were much higher in DED participants than in normal controls (P < 0.001). The IB frequency was closely associated with DED symptoms and signs (|R| ≥ 0.195, P ≤ 0.048), as was IB proportion and the average IPH (R ≥ 0.202, P ≤ 0.042). Conclusions: DLM is a powerful tool for analyzing blink videos with high accuracy and sensitivity, and a frame rate ≥ 30 FPS is recommended. The IB frequency is indicative of DED. Translational Relevance: The system of DLM-based blink analysis is of great potential for the assessment of IB and diagnosis of DED.

Calcium-modified granular attapulgite removed phosphorus from synthetic wastewater containing low-strength phosphorus.

Traditional biological processes combined with chemical precipitation methods can effectively reduce phosphate concentration in wastewater. However, discharge standards required additional advanced treatment technologies, and the removal of low phosphorus concentration is complicated and expensive. This study proposes application of a simple and recyclable adsorbent to remove low-concentration phosphorus from water. The removal efficiency of phosphorus from low-strength synthetic wastewater was investigated and the adsorption mechanism was analyzed. When the initial phosphorus concentration was 2.0 mg/L, the phosphorus adsorption capacity of Ca-GAT increased to 0.891 mg/g from 0.074 mg/g for GAT at 298 K and pH of 7. Phosphorus adsorption on Ca-GAT performs well when the solution pH is in the range of 5-10, and it is not conducive to the adsorption reaction when the solution pH exceeds 11. The competing anions (such as NO3-, SO42-, HCO3- and F-) existed, Ca-GAT still performed better in removing phosphorus. Then, the saturated absorbents could be effectively regenerated with a 0.5 mos/L NaOH solution, while desorption efficiency was reduced from 97.11% to 33.06% after fifth regeneration cycle. Finally, Scanning electron microscopy-energy dispersive spectroscopy (SEM-EDS), Fourier-transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS) analysis demonstrated that the Ca2+ content on the Ca-GAT surface played an important role in capturing phosphate ions from wastewater. Phosphorus was mainly removed via the formation of Ca-phosphorus precipitation. To some extent, ligand exchanges of CO32- and OH- with HPO42- and H2PO4- were also beneficial for phosphorus removal. The present work shows that attapulgite has sustainable and beneficial potential in the removal of low-strength phosphorous in wastewater, and the phosphorus loaded adsorbent can be used in the agriculture as slow-release fertilizer.

Pathway analysis of the biodegradation of lignin by Brevibacillus thermoruber.

With increased interest in the biodegradation of lignin, there is a pressing need to evaluate the feasibility of using microorganisms for lignin degradation. A novel Bacillus strain was separated from compost and identified as Brevibacillus thermoruber. B. thermoruber showed excellent performance in lignin degradation and degraded 81.97% of lignin after 7 d, which was similar to the lignin degradation rate of fungi. The biodegradation of lignin G and H monomers mainly proceeded via the ß-ketoadipate pathway at 37 °C. At 55 °C, the degradation product of lignin S monomer was mainly a benzoic acid substance, indicating that the lignin was degraded via the benzoic acid pathway. The degradation products of lignin are important precursors for humus formation in compost. The results of this study provide new insights into the biodegradation pathway of lignin in different stages of composting.

Synthesis of Fe0/Fe3O4@porous carbon through a facile heat treatment of iron-containing candle soots for peroxymonosulfate activation and efficient degradation of sulfamethoxazole.

Developing highly efficient, reusable, non-toxic and low-cost catalysts is of great importance for persulfate-based advanced oxidation processes (AOPs). In this work, ferrocene was mixed into paraffin to prepare a candle, and the iron-containing candle soots were collected and heated at 500 °C~900 °C under N2 atmosphere for 1 h to prepare magnetically recyclable Fe0/Fe3O4@porous carbon (Fe0/Fe3O4@PC) catalysts. The Fe0/Fe3O4@PC-700 obtained after pyrolysis at 700 °C exhibited the best catalytic activity for sulfamethoxazole (SMX) degradation. 10 mg/L SMX could be completely degraded within 10 min by 0.2 g/L of Fe0/Fe3O4@PC-700 and 0.5 mM PMS at pH 5.0. The carbon shell effectively inhibited the Fe leaching of Fe0/Fe3O4@PC-700, and 99.73% of Fe was retained after five consecutive cycles. In the Fe0/Fe3O4@PC-700/PMS system, SMX was degraded through the sulfate radical (SO4·¯), hydroxyl radical (·OH), superoxide radical (O2·¯) dominated radical pathway, and the singlet oxygen (1O2) dominated non-radical pathway. The coexisting inorganic ions and natural organic matters (NOM) in actual water inhibited the degradation of SMX. Finally, four possible degradation pathways were proposed based on the degradation intermediates of SMX. This work provides a facile heat treatment of iron-containing candle soots strategy to prepare the metal@carbon catalysts for PMS-based AOP.

A triple-chamber microbial fuel cell enabled to synchronously recover iron and sulfur elements from sulfide tailings.

Bioleaching by coupling iron oxidization with microbial growth is a process frequently used to extract target metals from sulfide tailing piles. However, the slower leaching, longer operational times, and lower efficiency compared to those of other extracting processes are the most important reasons that make this approach unattractive for the recovery of target elements. A triple-chamber microbial fuel cell (MFC) was explored to elevate the dissolution of sulfide tailings via in-situ removal of bioleached Fe3+/Fe2+ and SO42-, during which iron and SO42- ions were synchronously recovered as Fe(OH)3 and S° in the first and second cathode chambers, respectively. 107.9 % of iron and 99.8 % of sulfur contained in the sulfide tailings was bioleached over 50 h, with 80.0 % iron and 22.1 % sulfur elements synchronously recovered. The purities of the Fe(OH)3 and S° precipitates with high metallurgical values were up to 93.1 % and 90.2 %, respectively. The excellent leaching performance of the explored triple-chamber MFC was attributed to the synergistic effect of Acidithiobacillia catalysis and electrochemical oxidation. The explored approach, by virtue of having the higher bioleaching efficiency, less aggressive conditions and shorter operating times than the conventional bioleaching, is of potential commercial value.

Deep anterior lamellar keratoplasty with cross-linked acellular porcine corneal stroma to manage fungal keratitis.

PURPOSE: To evaluate the effects of deep anterior lamellar keratoplasty (DALK) with cross-linked acellular porcine corneal stroma (APCS) and post-operative topical tacrolimus treatment in patients with fungal keratitis. METHODS: This multicenter prospective study involved 25 cases of fungal keratitis that were treated by DALK with cross-linked APCSs and post-operative topical tacrolimus from December 2013 to November 2014 at the Wenzhou Eye Hospital and the Henan provincial Eye Hospital. Signs of post-operative inflammation, corneal reepithelialization, corneal neovascularization, and graft rejection were assessed, and best corrected visual acuity (BCVA), intraocular pressure (IOP), and APCS graft transparency were monitored for the 12-month follow-up period. RESULTS: All 25 patients underwent DALK without Descemet's membrane perforation. Corneal epithelium recovered completely in 17 patients in the first week, and APCS grafts maintained transparency in 18 patients at 1-year follow-up. The mean BCVA significantly improved from 2.16 ± 0.32 (LogMAR) at baseline to 1.56 ± 0.70 at 1-week (P < .001), 0.95 ± 0.57 at 1-month (P < .001), and 0.70 ± 0.51 at 3-month follow-ups (P < .001). The BCVA kept stable at 6-month and 12-month follow-ups. Post-operative topical tacrolimus alleviated the ciliary injection, except in one case which acute stromal rejection occurred. One patient developed fungal reinfection and underwent penetrating keratoplasty. Graft rejection occurred in three patients. No case was noted with graft splitting, elevated IOP or tacrolimus intolerance. CONCLUSIONS: DALK using cross-linked APCS combining topical tacrolimus treatment is safe and effective in managing fungal keratitis. It may ameliorate the shortage of corneal donation globally.

Thermally treated candle soot as a novel catalyst for hydrogen peroxide in-situ production enhancement in the bio-electro-Fenton system.

Thermally treated candle soot (TCS) was used as a two-electron (2e¯) oxygen reduction reaction (ORR) catalyst to in situ produce H2O2 in a bio-electro-Fenton (BEF) system. Compared with the pristine candle soot (CS), TCS showed larger Brunauer-Emmett-Teller (BET) surface area (102.54 m2 g-1 vs. 61.79 m2 g-1), higher mesoporous ratio (50.39% vs. 34.98%), and improved hydrophilicity. X-ray photoelectron spectra (XPS) results revealed that the C-O-C was the dominant oxygen-containing group of the CS, and its percentage reached at 80.55%. However, the C-O-C ratio of the TCS decreased to 48.93%, whilst it's CO and OC-O ratios significantly increased to 27.92% and 23.15%. The TCS showed a high H2O2 selectivity (87.5%∼97.0%) at the neutral pH condition, which was much higher than that of the commonly used carbon black (CB) catalyst. Finally, the H2O2 concentration maxima (Cmax-H2O2) of the bio-electro-Fenton system running with the TCS air-cathode (BEF-TCS) achieved at 32.02 mg/L, which was 6.29 times higher than that of the BEF-CB (5.09 mg/L). The removal and mineralization ratios of the SMX in the BEF-TCS reached at 83.0% and 79.0%, respectively. This paper reported a novel 2e¯ ORR electro-catalyst which was low-cost, easily available and highly efficiency.

Self-generated electric field to suppress sludge production and fouling development in a membrane bioreactor for wastewater treatment.

Compared with conventional sludge reduction techniques, electric field assisted membrane bioreactor (MBR) is a cost-effective technology with low power consumption. In this study, spontaneous electric field without extra power supply was introduced into the MBR for wastewater treatment to complete the in situ sludge reduction and membrane fouling mitigation. A novel spontaneous electric field membrane bioreactor (SEF-MBR) equipped with Cu-nanowires (Cu-NWs) conductive microfiltration membrane as cathode was established by using baffles to form anaerobic and aerobic tanks. SEF-MBR 1 with external resistance of 500 Ω maintained a highest electric field intensity of 1.25 mV/cm. Compared with Control-MBR, the reduction of mixed liquor suspended solids (MLSS) growth rate, extracellular polymeric substances (EPS) growth rate, total cell number and water content of SEF-MBR 1 reached 50.0%, 43.0%, 37.1% and 6.4%, respectively. After 43 days operation, SEF-MBR 1 obtained the minimum MLSS concentration and sludge volume, which were 29.9% and 83.8% lower than that of it in Control-MBR. The total biovolume of the contaminants (i.e., EPS and cells) on the membrane surface of SEF-MBR 1 was 68.8% lower than that of Control-MBR. SEF-MBR 1 exhibited a better performance with a lower membrane fouling rate (0.58 kPa/d) than Control-MBR (1.09 kPa/d). Economic analysis showed that a total of 148.1 kWh/m3 of electric energy was saved in the SEF-MBR 1. This technology reduced the sludge production in the sewage biological treatment process, which realized the sludge reduction had a positive impact on the membrane fouling mitigation.

Substance P induces sympathetic immune response in the contralateral eye after the first eye cataract surgery in type 2 diabetic patients.

BACKGROUND: Substance P (SP) is a nociceptive tachykinin which regulates the immune inflammatory reactions including monocyte chemoattractant protein 1 (MCP-1) production. Sequential second-eye cataract surgery patients often suffer more pain than the first one partly because of the MCP-1 increase in aqueous humor (AH). This study aims to illustrate whether SP is involved in sympathetic inflammatory responses in the contralateral eye in patients with or without type 2 diabetes. METHODS: This prospective randomized clinical study included 51 cataract patients (22 with diabetes and 29 without). Bilateral sequential cataract surgeries were conducted with 1-day or 1-week interval randomly. More than 100 µl of AH were obtained before surgery and stored for later analysis using magnetic Luminex assays to detect interleukin (IL)-1ß, IL-1ra,IL-6, IL-8, IL-10, MCP-1, vascular endothelial growth factor, spinal macrophage inflammatory protein (MIP-1a), interferon-inducible protein 10 (IP-10), regulated on activation, normal T expressed and secreted (RANTES), as well as the enzyme-linked immunosorbent assay for SP. RESULTS: Among the 4 groups, no significant differences were found in age, sex distribution, the R/L ration of the first surgery eye, or the lens nucleus hardness (P ≥ 0.802). Over 100 µl of AH samples were collected safely in all cases without intraoperative complications. SP and MCP-1 levels were both increased significantly in the second eye of the diabetic patients with 1-day and 1-week interval (P ≤ 0.040). The SP increase in the second eye of the diabetic patients were significantly higher than that of the patients without diabetes (P ≤ 0.030) both in the groups with 1-day and 1-week interval. Similarly, the MCP-1 increase was significantly higher in the diabetic patients in the group with 1-week interval (P = 0.042). CONCLUSIONS: Substance P and MCP-1 productions elevated in the AH of the contralateral eye after the first-eye cataract surgery in diabetic patients, indicating that SP and MCP-1 were involved in the sympathetic inflammatory responses. Diabetic patients are susceptible to noninfectious inflammation after cataract surgery, and perceive more pain in the second-eye phacoemulsification. TRIAL REGISTRATION: Chinese Clinical Trial Registry, ChiCTR1900028374 , retrospectively registered on 20th December, 2019.

Effect of heterotrophic anodic denitrification on anolyte pH control and bioelectricity generation enhancement of bufferless microbial fuel cells.

Heterotrophic anodic denitrification (HAD) in the single-chamber microbial fuel cell (MFC) is a promising nitrogen removal technology. In this paper, the benefit (anolyte pH increase) and challenge (substrate consumption) brought by the heterotrophic anodic denitrification process for the electricity generation of bufferless MFCs were studied for the first time. Substrate anaerobic hydrolysis dramatically decreased the anolyte pH to 5.1, which seriously restricted the electric power output of the Control. The anolyte pH of the heterotrophic anodic denitrification MFCs (HADMFCs) with 60 mg/L (HADMFC-60), 90 mg/L (HADMFC-90), and 120 mg/L (HADMFC-120) nitrate nitrogen (NO3--N), retained above 6.0, 6.5, and 6.8 in every running cycles, due to the protons (H+) consumption by nitrate reduction. In the HADMFC-60 and HADMFC-90, 17.6% and 26.1% of the total organic carbons (TOC) were used for the nitrate reduction, but their electric power output significantly increased. The maximum power densities of the HADMFC-60 and HADMFC-90 were 3.3 and 5.4 times higher than that of the Control. However, when the proportion of TOC consumption for nitrate reduction increased to 35.8%, substrate insufficiency became a serious limitation for the electricity generation. The Pmax of the HADMFC-120 dramatically decreased to 17.3 mW/m2. Dysgonomonas was the dominant electro-active genus, and Petrimonas, Acidovorax and Devosia appeared as the denitrifying bacteria genera.