Superhydrophobic polypyrene films to prevent Staphylococcus aureus and Pseudomonas aeruginosa biofilm adhesion on surfaces: high efficiency deciphered by fluorescence microscopy.

A blue luminescent and superhydrophobic coating based on an electropolymerized fluorinated-pyrene monomer and its planktonic bacteria and biofilm repellent properties are reported. Two different pathogenic bacterial strains (Gram-positive and Gram-negative) at two different incubation times (2 h planktonic bacterial and 24 h biofilm adhesion) were studied and monitored (analyzed) using multicolor scanning confocal fluorescence microscopy. The coating was proved to reduce bacterial adhesion by 65%. It is highly effective against biofilm attachment, with 90% reduction of bacteria surface coverage. This blue fluorescent surface provides a facile method to characterize the coating, observe the bacterial distribution and quantify the bacterial coverage rate by fluorescence imaging of different colors. Furthermore, the film does not show significant bacterial toxicity during the working incubation times.

Finite-size effects and dynamics of giant transition of a continuum quorum percolation model on random networks.

We start from a continuous extension of a mean field approach of the quorum percolation model, accounting for the response of in vitro neuronal cultures, to carry out a normal form analysis of the critical behavior. We highlight the effects of nonlinearities associated with this mean field approach even in the close vicinity of the critical point. Statistical properties of random networks with Gaussian in-degree are related to the outcoming links distribution. Finite size analysis of explicit Monte Carlo simulations enables us to confirm the relevance of the mean field approach on such networks and to show that the order parameter is weakly self-averaging; dynamical relaxation is investigated. Furthermore we derive a mean field equation taking into account the effect of inhibitory neurons and discuss the equivalence with a purely excitatory network.

Electrochemical monitoring of the fluorescence emission of tetrazine and bodipy dyes using total internal reflection fluorescence microscopy coupled to electrochemistry.

A very sensitive technique where an electrochemical cell is coupled to a total internal reflection fluorescence microscopy setup is described and applied for the first time to the electrochemical monitoring of the fluorescence of organic dyes in solution. It is shown that this setup basically allows both spatial and time resolution for the recorded fluorescence signal as a function of the electrode potential: indeed the variations of the emission intensity are recorded within the diffusion layer for a classical cyclic voltammetry or chronoamperometry experiment inducing the redox conversion of an emissive form into a non emissive one (and conversely). Simultaneously, the variations of the emissive state lifetime are measured to discriminate between a mechanism involving only the conversion into a non emissive form from one involving a quenching between the emitter and the electrogenerated species. The results concerning the investigation of the electrochemical monitoring of the fluorescence properties for two types of original dyes are presented, demonstrating the possibility to switch on and off the emission in a fully reversible way and to investigate in depth the mechanisms associated to this switch.

Sudden cardiac arrest due to puerperal transient left ventricular apical ballooning syndrome.

A 37 year old woman with chest pain was admitted to the emergency room 40 days after normal delivery with ventricular fibrillation due to Takotsubo cardiomyopathy.

Scanning-less wide-field single-photon counting device for fluorescence intensity, lifetime and time-resolved anisotropy imaging microscopy.

A scanning-less single-photon counting system for FLIM and fluorescence anisotropy wide-field imaging is described and characterized in this paper. The two polarizations of the fluorescence are divided by a Glan prism and acquired at the same time by the Q(A) detector. Fluorescence decay profiles can be reconstructed for any desired area up to each pixel and used to calculate time-resolved fluorescence anisotropy decays.