Myeloid marker S100A8/A9 and lymphocyte marker, soluble interleukin 2 receptor: biomarkers of hidradenitis suppurativa disease activity?

BACKGROUND: Hidradenitis suppurativa (HS) is a chronic inflammatory and debilitating disease of the skin. No biomarkers for this disease exist. OBJECTIVES: We set out to test whether angiotensin-converting enzyme (ACE), lysozyme, soluble interleukin 2 receptor (sIL-2R) and S100A8/A9 (calprotectin) are elevated in patients with HS. METHODS: Serum was collected from 29 patients with HS at different stages of the disease, and from 51 controls. ACE, lysozyme, sIL-2R and S100A8/A9 levels were measured. Clinical observation of disease activity was scored according to the Hurley grading system and by a physician global score (PGS) of disease severity. RESULTS: Serum levels of lysozyme and ACE were not increased above the normal reference values in controls or patients with HS. Levels of sIL-2R and S100A8/A9 were significantly higher in patients with HS than in controls (P<0·001 for both sIL-2R and S100A8/A9). Based on the receiver operating characteristic curves, the optimum sIL-2R and S100A8/A9 cut-off values were 375 U mL(-1) and 680 ng mL(-1), respectively, with a sensitivity of 0·79 and specificity of 0·78 for sIL-2R, and 0·86 and 0·88, respectively, for S100A8/A9. No correlations with Hurley classification scores were found. However, when using PGS of disease activity to categorize patients, levels of S100A8/A9, but not sIL-2R, tended to be higher in patients with more active disease. CONCLUSIONS: Levels of S100A8/A9 and sIL-2R, but not ACE or lysozyme, are elevated in the serum of patients with HS. However, there is no correlation between S100A8/A9 or sIL-2R levels and disease stage according to the Hurley classification system. Further research is needed to study the potential of S100A8/A9 to score disease activity in larger cohorts of patients and to predict disease flares.

Adult Phenotypes in Angelman- and Rett-Like Syndromes.

BACKGROUND: Angelman- and Rett-like syndromes share a range of clinical characteristics, including intellectual disability (ID) with or without regression, epilepsy, infantile encephalopathy, postnatal microcephaly, features of autism spectrum disorder, and variable other neurological symptoms. The phenotypic spectrum generally has been well studied in children; however, evolution of the phenotypic spectrum into adulthood has been documented less extensively. To obtain more insight into natural course and prognosis of these syndromes with respect to developmental, medical, and socio-behavioral outcomes, we studied the phenotypes of 9 adult patients who were recently diagnosed with 6 different Angelman- and Rett-like syndromes. METHODS: All these patients were ascertained during an ongoing cohort study involving a systematic clinical genetic diagnostic evaluation of over 250, mainly adult patients with ID of unknown etiology. RESULTS: We describe the evolution of the phenotype in adults with EHMT1, TCF4, MECP2, CDKL5, and SCN1A mutations and 22qter deletions and also provide an overview of previously published adult cases with similar diagnoses. CONCLUSION: These data are highly valuable in adequate management and follow-up of patients with Angelman- and Rett-like syndromes and accurate counseling of their family members. Furthermore, they will contribute to recognition of these syndromes in previously undiagnosed adult patients.

Spiraling bubbles: how acoustic and hydrodynamic forces compete.

Experiments to study the effect of acoustic forces on individual bubbles in shear flows have been carried out. In the system that we have used, the competition between acoustic and fluid dynamical forces results in a spiraling bubble trajectory. This dynamics is modeled by expressing the balance between Bjerknes and hydrodynamic forces in terms of an ordinary differential equation model, to which a separation of time scales is applied. The success of this model shows that the simple force-balance approach is still meaningful when bubbles are subjected to sound fields.

Photosynthetic electron transport inhibition by pyrimidines and pyridines substituted with benzylamino, methyl and trifluoromethyl groups.

The decrease of the number of ring nitrogen atoms of 2-benzylamino-4-methyl-6-trifluoromethyl-1,3,5-triazines on herbicidal activity and inhibition of photosynthetic electron transport (PET) was assayed using thylakoids from Spinacia oleracea or atrazine-resistant Chenopodium album. Three 2-benzylamino-4-methyl-6-trifluoromethyl-1,3,5-triazines, nine pyrimidines with a benzylamino-, methyl- and trifluoromethyl-group, 2-benzylamino-6-methyl-4-trifluoromethyl- pyridine and N-benzyl-3-methyl-5-trifluoromethylaniline were synthesized and assayed. 2-(4-Bromobenzylamino)-4-methyl-6-trifluoromethylpyrimidine exhibited the highest PET inhibitory activity against Spinacia oleracea thylakoids of all compounds tested. The 2-benzylaminopyrimidines and 2-methylpyrimidines having a 4-halobenzylamino group exhibited higher PET inhibition than atrazine and 2-trifluoromethylpyrimidines against Spinacia oleracea thylakoids. These PET inhibitory active compounds also exhibited a strong and similar inhibition both against atrazine-resistant Chenopodium album thylakoids as well as against thylakoids from wild-type Chenopodium. The herbicidal activity of 4-(4-bromobenzylamino)-2-methyl-6- trifluoromethylpyrimidine was equivalent to that of known herbicides like simetryne, simazine or atrazine.

Multiple functions of photosystem II.

The most important function of photosystem II (PSII) is its action as a water-plastoquinone oxido-reductase. At the expense of light energy, water is split, and oxygen and plastoquinol are formed. In addition to this most important activity, PSII has additional functions, especially in the regulation of (light) energy distribution. The downregulation of PSII during photoinhibition is a protection measure. PSII is an anthropogenic target for many herbicides. There is a unique action of bicarbonate on PSII. Decrease in the activity of PSII is the first effect in a plant under stress; this decreased activity can be most easily measured with fluorescence. PSII is a sensor for stress, and induces regulatory processes with different time scales: photochemical quenching, formation of a proton gradient, state transitions, downregulation by photoinhibition and gene expression.

Low resistance against novel 2-benzylamino-1,3,5-triazine herbicides in atrazine-resistant Chenopodium album plants.

The effects of nine novel 2-benzylamino-1,3,5-triazines on photosynthetic reactions were measured in thylakoids isolated from wild-type and atrazine-resistant plants of Chenopodium album. The resistant plants have a mutation of serine for glycine at position 264 of the D1 protein. The measurement of oxygen evolution and chlorophyll a fluorescence induction indicated a 2-4-fold stronger inhibition by the 6-trifluoromethyl analogues of Photosystem II-dependent electron flow than atrazine. Analogues having a 6-methyl-, 6-monofluoromethyl or 6-difluoromethyl substitution were weak inhibitors, indicating that the 6-trifluoro group is very important for strong inhibition. All the nine novel 2-benzylamino-1,3,5-triazines were almost as active in wild-type as in atrazine-resistant thylakoids, indicating that the benzylamino substitution may be important for the lack of resistance in the atrazine-resistant plants.

Characterization of the complex interaction between the electron acceptor silicomolybdate and Photosystem II.

Silicomolybdate (SiMo) and its effects on thylakoids have been characterized to evaluate its use as a probe for Photosystem II (PS II). It can accept electrons at two places in the electron transport chain: one at PS II and the other at PS I. In the presence of 1 µM 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB) only the site at PS II is available. It is suggested that SiMo must disp;ace bicarbonate from its binding site to be able to function as an electron acceptor. This displacement is non-competitive. The binding of SiMo is inhibited differentially by PS II inhibitors: dinoseb>ioxynil> diuron. This difference is determined by the different positions of the inhibitors within the QB binding niche and their interaction with bicarbonate. The experimental results show that the SiMo-binding niche is located between the parallel helices of the D1 and D2 proteins of PS II, close to the non-heme iron. We conclude that SiMo is an electron acceptor with unique characteristics useful as a probe of the acceptor side of PS II.

High misses after odd flashes in oxygen evolution in thoroughly dark-adapted thylakoids from pea and Chenopodium album.

In Photosystem II (PS II), water is oxidized to molecular oxygen and plastoquinone is reduced to plastoquinol. The oxidation of water requires the accumulation of four oxidizing equivalents, through the so-called S-states of the oxygen evolving complex; the production of plastoquinol requires the accumulation of two reducing equivalents on a bound plastoquinone, QB. It has been generally believed that during the flash-induced transition of each of the S-states (Sn → Sn+1, where n=0, 1, 2 and 3), a certain small but equal fraction of the PS II reaction centers are unable to function and, thus, 'miss' being turned over. We used thoroughly dark-adapted thylakoids from peas (Pisum sativum) and Chenopodium album (susceptible and resistant to atrazine) starting with 100% of the oxygen evolving complex in the S1 state. Thylakoids were illuminated with saturating flashes, providing a double hit parameter of about 0.07. Our experimental data on flashnumber dependent oscillations in the amount of oxygen per flash fit very well with a binary pattern of misses: 0, 0.2, 0, 0.4 during S0 → S1, S1 → S2, S2 → S3 and S3 → S0 transitions. Addition of 2 mM ferricyanide appears to shift this pattern by one flash. These results are consistent with the 'bicycle' model recently proposed by V. P. Shinkarev and C. A. Wraight (Oxygen evolution in photosynthesis: From unicycle to bicycle, 1993, Proc Natl Acad Sci USA 90: 1834-1838), where misses are due to the presence of P(+) or QA (-) among the various equilibrium states of PS II centers.

A rapid method for partial mRNA and DNA sequence analysis of the photosystem IIpsbA gene.

Single amino acid substitutions in the D1 protein of photosystem II may cause resistance to various herbicides. In all organisms studied these substitutions are located in or between helices IV and V of the protein. The increasing number of herbicide-resistant organisms necessitates development of a rapid methodology to characterize deviations from the wildtype sequence. Here, two procedures are described to identify mutations in the psbA gene, which is coding for D1. These procedures involve the isolation and amplification of DNA and RNA and subsequent sequencing reactions without the need to clone the psbA gene. A triazine-resistant and a -susceptible biotype of Chenopodium album were used as model species. An A to G transition, giving rise to a serine to glycine mutation at position 264 in the D1 protein, is found in the resistant plant.

Regulation of photosynthetic electron transport by bicarbonate formate and herbicides in isolated broken and intact chloroplasts.

In this paper, we have presented a minireview on the interaction of bicarbonate, formate and herbicides with the thylakoid membranes.The regulation of photosynthetic electron transport by bicarbonate, formate and herbicides is described. Bicarbonate, formate, and many herbicides act between the primary quinone electron acceptor QA and the plastoquinone pool. Many herbicides like the ureas, triazines and the phenol-type herbicides act, probably, by the displacement of the secondary quinone electron acceptor QB from its binding site on a QB-binding protein located at the acceptor side of Photosystem II. Formate appears to be an inhibitor of electron transport; this inhibition can be removed by the addition of bicarbonate. There appears to be an interaction of the herbicides with bicarbonate and/or It has been suggested that both the binding of a herbicide and the absence of bicarbonate may cause a conformational alteration of the environment of the QB-binding site. The alteration brought about by a herbicide decreases the affinity for another herbicide or for bicarbonate; the change caused by the absence of bicarbonate decreases the affinity for herbicides. Moreover, this change in conformation causes an inhibition of electron transport. A bicarbonate-effect in isolated intact chloroplasts is demonstrated.

Reevaluation of the role of bicarbonate and formate in the regulation of photosynthetic electron flow in broken chloroplasts.

The stimulation of the Hill reaction in CO(2)-depleted broken chloroplasts (Pisum sativum L. cv Rondo) by the total amount of dissolved CO(2) and HCO(3) (-) (bicarbonate(*)) was measured at several formate concentrations. Formate appears to be a competitive inhibitor of the bicarbonate(*) stimulation of electron flow. From these experiments we have obtained a reactivation constant (K(r)) of 78 +/- 31 micromolar NaHCO(3) and an inhibition constant (K(i)) of 2.0 +/- 0.7 millimolar HCOONa at pH 6.5. In the absence of formate, significant electron flow was measured at a bicarbonate(*) concentration well below K(r), suggesting that electron flow from Q, the primary electron acceptor of photosystem II, to plastoquinone can proceed when no bicarbonate(*) is bound to the regulatory site at the Q(B)-protein. If so, bicarbonate(*) stimulation of electron flow is mainly a diminution of the inhibition of electron flow by formate. In view of the results, it is proposed that regulation of linear electron flow by bicarbonate(*) and formate is a mechanism that could link cell metabolism to photosynthetic electron flow.

Mechanism of bicarbonate action on photosynthetic electron transport in broken chloroplasts.

In CO2-depleted chloroplasts electron transport between the Photosystem II electron acceptor Q and plastoquinone is largely suppressed. In the presence of a high concentration of sodium formate (greater than 10 mM), which probably binds to the bicarbonate site, addition of bicarbonate restores the ferricyanide Hill reaction only after incubation in the dark. With lower formate concentrations bicarbonate is able to restore electron transport in the light. The Hill reaction rate in CO2-depleted chloroplasts after bicarbonate addition, divided by the rate in CO2-depleted chloroplasts before bicarbonate addition, shows a sharp optimum at pH 6.5. Furthermore, the rate-limiting step in bicarbonate action is probably diffusion. The results are explained in terms of a hypothetical model: the bicarbonate-binding site is located at the outer side of the thylakoid membrane, but not directly accessible from the "bulk". To reach the site from the bulk, the molecule has to pass a channel with negatively charge groups on its side walls. In the light these groups are more negatively charged than in the dark. Therefore, the formate ion cannot exchange for bicarbonate in the light, and a dark period is necessary to enable exchange of formate for bicarbonate.

Evidence for a close spatial location of the binding sites for CO2 and for photosystem II inhibitors.

1. CO2-depletion of thylakoid membranes results in a decrease of binding affinity of the Photosystem II (PS II) inhibitor atrazine. The inhibitory efficiency of atrazine, expressed as I50-concentration (50% inhibition) of 2,6-dichlorophenolindophenol reduction, is the same in CO2-depleted as well as in control thylakoids. This shows that CO2-depletion results in a complete inactivation of a part of the total number of electron transport chains. 2. A major site of action of CO2, which had previously been located between the two electron acceptor quinone molecule B (or R) and Photosystem II inhibitor atrazine as suggested by the following observations: (a) CO2-depletion results in a shift of the binding constant (kappa b) of [14C]atrazine to thylakoid membranes indicating a decreased affinity of atrazine to membrane; (b) trypsin treatment, which is known to modify the Photosystem II complex at the level of B, strongly diminishes CO2 stimulation of electron transport reactions in CO2-depleted membranes; and (c) thylakoids from atrazine-resistant plants, which contain a Photosystem II complex modified at the inhibitor binding site, show an altered CO2-stimulation of electron flow. 3. CO2-depletion does not produce structural changes in enzyme complexes involved in Photosystem II function of thylakoid membranes, as shown by freeze-fracture studies using electron microscopy.