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1.
Exp Dermatol ; 27(2): 150-155, 2018 02.
Artigo em Inglês | MEDLINE | ID: mdl-29152798

RESUMO

Skin mechanical properties are usually measured considering the entire skin thickness and very little is known about the mechanical behaviour of individual skin layers. We propose atomic force microscopy (AFM) as a tool to quantify nanoscale changes in the biomechanical properties and ultrastructure of human papillary dermis exposed to different mechanical and physical stimuli. Samples from 3 human skin biopsies were studied: one stretched by obesity, one subjected to a high level of sun exposure and normal skin as control. Slices of the papillary dermis layer were harvested at controlled depths from each skin biopsy and 25 µm2 areas of each slice were imaged and D-periodicity of collagen fibres measured by AFM, together with their stiffness. Standard histological analysis was also carried out to correlate biochemical properties and their distribution with stiffness and topography. We obtained similar stiffness values between the sample affected by obesity and the control sample at any depth level into the dermis, while the sun-exposed sample presented a significantly lower stiffness. Additionally, all samples presented an increase in the stiffness at higher depths into the papillary dermis layer. Collagen fibres close to the epidermis of sample affected either by obesity and sun exposure-the former even more than the latter-are thicker and present a larger D-period than those in the control sample. Our results open the possibility to use structural and mechanical analysis based on AFM as a complementary tool for medical diagnosis and therapy monitoring.


Assuntos
Derme/patologia , Epiderme/patologia , Microscopia de Força Atômica , Fenômenos Biomecânicos , Biópsia , Derme/diagnóstico por imagem , Derme/efeitos da radiação , Elasticidade , Humanos , Obesidade/complicações , Obesidade/metabolismo , Pele/patologia , Estresse Mecânico , Queimadura Solar/complicações
2.
Aesthet Surg J ; 37(1): 14-23, 2017 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-27241362

RESUMO

BACKGROUND: Adipose-derived stromal and stem cells (ADSC) in autologous fat promises regenerative advantages, and injected into the dermal and subdermal layers, enhances rejuvenation and volume. However, extremely superficial fat injection with current techniques is limited. OBJECTIVES: Efficacy and viability evaluation of fat harvested with extremely small side port (0.3 mm) cannulae without further tissue manipulation for the correction of aging/thin skin in the periocular and perioral regions. METHODS: Micro-superficial enhanced fluid fat injection (M-SEFFI) harvests adipose tissue with a multi-perforated cannula (0.3 mm), and autologous platelet rich plasma (PRP) is added. The tissue is injected into the dermal region of the periocular and perioral zones. Efficacy and viability were evaluated by histological and cell culture analysis. Clinical assessment included retrospective evaluation according to 1 = no effect, 2 = fair effect, 3 = good effect, 4 = excellent effect. RESULTS: Between June 2014 and July 2015, 65 patients (7 men; mean age 49.7 years) were treated with M-SEFFI. No intraoperative complications or visible lumpiness were recorded. Analysis demonstrated mature, viable adipocytes with a strong stromal component. Following PRP addition, there was a greater proliferation noted in the M-SEFFI compared to the SEFFI (0.5 mm). Mean follow-up was 4.1 months. Clinical assessment by surgeons and patients at 1 month was 3.52 and 3.74, and 6 months 3.06 and 2.6 respectively. CONCLUSIONS: M-SEFFI is effective and viable for lump free skin rejuvenation and volume enhancement, through the extraction of smoother ADSC rich, autologous fat tissue that does not require further tissue manipulation, to correct skin aging. LEVEL OF EVIDENCE: 4 Therapeutic.


Assuntos
Tecido Adiposo/transplante , Técnicas Cosméticas , Olho , Boca/cirurgia , Rejuvenescimento , Envelhecimento da Pele , Transplante de Células-Tronco , Adulto , Idoso , Sobrevivência Celular , Células Cultivadas , Técnicas Cosméticas/efeitos adversos , Estética , Olho/anatomia & histologia , Feminino , Humanos , Lipólise , Masculino , Microinjeções , Pessoa de Meia-Idade , Boca/anatomia & histologia , Fotografação , Complicações Pós-Operatórias/etiologia , Estudos Retrospectivos , Transplante de Células-Tronco/efeitos adversos , Fatores de Tempo , Coleta de Tecidos e Órgãos , Transplante Homólogo , Resultado do Tratamento
3.
Aesthet Surg J ; 36(3): 358-63, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-26499941

RESUMO

BACKGROUND: Adipose tissue transplantation has the benefit of providing both regenerative and aesthetic outcomes in breast cancer treatment. However, the transplanted tissue can stimulate the growth of residual cancer cells. OBJECTIVES: The aim of this study is to identify the interactions between adipose tissue cell subpopulations and human cancer cell lines. METHODS: Intact adipose tissue from lipofilling procedures as well as fibroblasts derived from adipose tissue, were cocultured in the presence of MDA-MB-231, MCF-7 e ZR-75-1 breast cancer cell lines. The influence on cancer cell lines of fibroblasts, induced to differentiate into specific adipocytes, was also assayed. RESULTS: All cancer cell lines displayed a significant increase in proliferation rate when cocultured in the presence of either intact adipose tissue or induced adipocytes. To a lesser extent, uninduced fibroblasts stimulate breast cancer cell proliferation. CONCLUSIONS: Recent studies have shown that the microenvironment surrounding breast cancer cells may stimulate growth and promote progression of residual cancer cells when surgery is performed on the main tumor mass. Accordingly, the graft of adipose tissue could potentially promote or accelerate the development of a subclinical tumor or support its locoregional recurrence. Our data suggest that adipocytes have a remarkable influence on the proliferation of cancer cell lines. The oncological safety of the lipofilling procedure outcome is still debated; thus, further studies and consistent follow-up examination are needed.


Assuntos
Adipócitos/fisiologia , Adipogenia , Tecido Adiposo/citologia , Neoplasias da Mama/patologia , Comunicação Celular , Transdiferenciação Celular , Fibroblastos/fisiologia , Adipócitos/metabolismo , Tecido Adiposo/transplante , Adulto , Neoplasias da Mama/metabolismo , Proliferação de Células , Técnicas de Cocultura , Feminino , Fibroblastos/metabolismo , Humanos , Células MCF-7 , Pessoa de Meia-Idade , Fenótipo
4.
Aesthet Surg J ; 35(5): 504-15, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-25911629

RESUMO

BACKGROUND: Although recent research on micro fat has shown the potential advantages of superficial implantation and high stem cell content, clinical applications thus far have been limited. OBJECTIVES: The authors report their experience with superficial enhanced fluid fat injection (SEFFI) for the correction of volume loss and skin aging of the face in general and in the periocular region. METHODS: The finer SEFFI preparation (0.5 mL) was injected into the orbicularis in the periorbital and perioral areas, and the 0.8-mL preparation was injected subdermally elsewhere in the face. RESULTS: The records of 98 consecutive patients were reviewed. Average follow-up time was 6 months, and average volume of implanted fat was 20 mL and 51.4 mL for the 0.5-mL and 0.8-mL preparations, respectively. Good or excellent results were achieved for volume restoration and skin improvement in all patients. Complications were minor and included an oil cyst in 3 patients. The smaller SEFFI quantity (0.5 mL) was well suited to correct volume loss in the eyelids, especially the deep upper sulcus and tear trough, whereas the larger SEFFI content was effective for larger volume deficits in other areas of the face, including the brow, temporal fossa, zygomatic-malar region, nasolabial folds, marionette lines, chin, and lips. CONCLUSIONS: The fat administered by SEFFI is easily harvested via small side-port cannulae, yielding micro fat that is rich in viable adipocytes and stem cells. Both volumes of fat (0.5 mL and 0.8 mL) were effective for treating age-related lipoatrophy, reducing facial rhytids, and improving skin quality. LEVEL OF EVIDENCE: 4 Therapeutic.


Assuntos
Adipócitos/transplante , Tecido Adiposo/transplante , Técnicas Cosméticas , Rejuvenescimento , Envelhecimento da Pele , Transplante de Células-Tronco , Tecido Adiposo/citologia , Adulto , Fatores Etários , Idoso , Autoenxertos , Diferenciação Celular , Células Cultivadas , Estética , Olho , Face , Feminino , Humanos , Injeções Intradérmicas , Lipólise , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Fatores de Tempo , Coleta de Tecidos e Órgãos/métodos , Resultado do Tratamento
5.
Biomed Res Int ; 2015: 524948, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25705670

RESUMO

Sarcopenia is defined as a syndrome characterized by progressive and generalized loss of muscle mass and strength. The more rationale approach to delay the progression of sarcopenia is based on the combination of proper nutrition, possibly associated with the use of dietary supplements and a regular exercise program. We performed a narrative literature review to evaluate the till-now evidence regarding (1) the metabolic and nutritional correlates of sarcopenia; (2) the optimum diet therapy for the treatment of these abnormalities. This review included 67 eligible studies. In addition to the well recognized link between adequate intake of proteins/amino acids and sarcopenia, the recent literature underlines that in sarcopenic elderly subjects there is an unbalance in vitamin D synthesis and in omega-6/omega-3 PUFA ratio. Given the detrimental effect of these metabolic abnormalities, a change in the lifestyle must be the cornerstone in the treatment of sarcopenia. The optimum diet therapy for the sarcopenia treatment must aim at achieving specific metabolic goals, which must be reached through accession of the elderly to specific personalized dietary program aimed at achieving and/or maintaining muscle mass; increasing their intake of fish (4 times/week) or taking omega-3 PUFA supplements; taking vitamin D supplementation, if there are low serum levels.


Assuntos
Suplementos Nutricionais , Músculo Esquelético/fisiopatologia , Sarcopenia/dietoterapia , Vitamina D/administração & dosagem , Idoso , Envelhecimento , Animais , Exercício Físico , Humanos , Estilo de Vida , Músculo Esquelético/efeitos dos fármacos , Sarcopenia/fisiopatologia
6.
Arch Gynecol Obstet ; 290(6): 1079-92, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25200687

RESUMO

INTRODUCTION: Polycystic ovary syndrome (PCOS) is associated with numerous metabolic morbidities (insulin resistance (IR), central obesity) and various nutritional abnormalities (vitamin D deficit, mineral milieu alterations, omega6/omega3 PUFA ratio unbalance). METHODS: We performed a systematic literature review to evaluate the till-now evidence regarding: (1) the metabolic and nutritional correlates of PCOS; (2) the optimum diet therapy for the treatment of these abnormalities. This review included 127 eligible studies. RESULTS: In addition to the well-recognized link between PCOS and IR, the recent literature underlines that in PCOS there is an unbalance in adipokines (adiponectin, leptin, visfatin) production and in omega6/omega3 PUFA ratio. Given the detrimental effect of overweight on these metabolic abnormalities, a change in the lifestyle must be the cornerstone in the treatment of PCOS patients. The optimum diet therapy for the PCOS treatment must aim at achieving specific metabolic goals, such as IR improvement, adipokines secretion and reproductive function. These goals must be reached through: accession of the patient to hypocaloric dietary program aimed at achieving and/or maintaining body weight; limiting the consumption of sugar and refined carbohydrates, preferring those with lower glycemic index; dividing the food intake in small and frequent meals, with high caloric intake at breakfast; increasing their intake of fish (4 times/week) or taking omega3 PUFA supplements; taking Vitamin D and chromium supplementation, if there are low serum levels. CONCLUSION: Lifestyle intervention remains the optimal treatment strategy for PCOS women. A relatively small weight loss (5 %) can improve IR, hyperandrogenism, menstrual function, fertility.


Assuntos
Adipocinas/sangue , Dieta , Ingestão de Energia , Obesidade/dietoterapia , Síndrome do Ovário Policístico/dietoterapia , Redução de Peso/fisiologia , Adulto , Feminino , Índice Glicêmico , Humanos , Hiperandrogenismo/complicações , Resistência à Insulina , Estilo de Vida , Obesidade/complicações , Obesidade/fisiopatologia , Sobrepeso/dietoterapia , Sobrepeso/fisiopatologia , Síndrome do Ovário Policístico/complicações , Síndrome do Ovário Policístico/psicologia , Redução de Peso/efeitos dos fármacos
7.
PLoS One ; 8(11): e79212, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24236111

RESUMO

The androgen receptor (AR) plays a central role in the development and progression of prostate cancer (PCa) and anti-androgen therapy is a standard treatment. Unfortunately, after a few years, the majority of patients progress, developing androgen-independent PCa. AR-driven gene transcription recruits a large number of co-activator/co-repressor complexes; among these, the heterogeneous nuclear ribonucleoprotein K (hnRNP K) directly interacts with and regulates the AR translational apparatus. Here we examined AR and hnRNP K expression in response to the treatment of LNCaP cells with anti-androgen cyproterone acetate (CPA) or bicalutamide (BIC). AR and hnRNP K modulation and compartmentalization were studied by Western blot and confocal microscopy. Phosphate-affinity gel electrophoresis was employed to examine how anti-androgens modified hnRNP K phosphorylation. 10(-6) M CPA significantly stimulated LNCaP proliferation, whereas for 10(-4) M CPA or 10(-5) M BIC an antagonistic effect was observed. After anti-androgen treatment, AR expression was remarkably down-regulated within both the cytoplasm and the nucleus; however, when CPA had an agonist activity, the AR associated with the nuclear matrix (NM) increased approximately 2.5 times. This increase was synchronous with a higher PSA expression, indicating that the NM-associated AR represents the active complex. After BIC treatment, hnRNP K expression was significantly lower in the NM, the protein was hypophosphorylated and the co-localization of AR and hnRNP K decreased. In contrast, CPA as an agonist caused hnRNP K hyperphosphorylation and an increase in the co-localization of two proteins. These findings demonstrate that, in vitro, there is a strong relationship between NM-associated AR and both cell viability and PSA levels, indicating that AR transcriptional activity is critically dependent on its subnuclear localization. Moreover, the agonistic/antagonistic activity of anti-androgens is associated with modifications in hnRNP K phosphorylation, indicating an involvement of this protein in the AR transcriptional activity and likely in the onset of the androgen-independent phenotype.


Assuntos
Ribonucleoproteínas Nucleares Heterogêneas Grupo K/metabolismo , Matriz Nuclear/metabolismo , Neoplasias da Próstata/metabolismo , Receptores Androgênicos/metabolismo , Antagonistas de Androgênios/farmacologia , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Humanos , Masculino , Fosforilação/efeitos dos fármacos , Neoplasias da Próstata/genética , Transporte Proteico
8.
PLoS One ; 7(7): e40617, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22808207

RESUMO

In tumor progression definite alterations in nuclear matrix (NM) protein composition as well as in chromatin structure occur. The NM interacts with chromatin via specialized DNA sequences called matrix attachment regions (MARs). In the present study, using a proteomic approach along with a two-dimensional Southwestern assay and confocal laser microscopy, we show that the differentiation of stabilized human prostate carcinoma cells is marked out by modifications both NM protein composition and bond between NM proteins and MARs. Well-differentiated androgen-responsive and slowly growing LNCaP cells are characterized by a less complex pattern and by a major number of proteins binding MAR sequences in comparison to 22Rv1 cells expressing androgen receptor but androgen-independent. Finally, in the poorly differentiated and strongly aggressive androgen-independent PC3 cells the complexity of NM pattern further increases and a minor number of proteins bind the MARs. Furthermore, in this cell line with respect to LNCaP cells, these changes are synchronous with modifications in both the nuclear distribution of the MAR sequences and in the average loop dimensions that significantly increase. Although the expression of many NM proteins changes during dedifferentiation, only a very limited group of MAR-binding proteins seem to play a key role in this process. Variations in the expression of poly (ADP-ribose) polymerase (PARP) and special AT-rich sequence-binding protein-1 (SATB1) along with an increase in the phosphorylation of lamin B represent changes that might trigger passage towards a more aggressive phenotype. These results suggest that elucidating the MAR-binding proteins that are involved in the differentiation of prostate cancer cells could be an important tool to improve our understanding of this carcinogenesis process, and they could also be novel targets for prostate cancer therapy.


Assuntos
Diferenciação Celular , Regiões de Interação com a Matriz , Proteínas Associadas à Matriz Nuclear/metabolismo , Neoplasias da Próstata/metabolismo , Neoplasias da Próstata/patologia , Especificidade de Anticorpos/imunologia , Sequência de Bases , Southwestern Blotting , Western Blotting , Linhagem Celular Tumoral , DNA de Neoplasias/química , Ribonucleoproteínas Nucleares Heterogêneas Grupo U/metabolismo , Humanos , Lamina Tipo B/metabolismo , Masculino , Proteínas de Ligação à Região de Interação com a Matriz/metabolismo , Conformação de Ácido Nucleico , Fosforilação , Poli(ADP-Ribose) Polimerases/metabolismo , Ligação Proteica , Transporte Proteico , Proteínas de Ligação a RNA/metabolismo
9.
Prostate ; 71(13): 1466-79, 2011 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-21321982

RESUMO

BACKGROUND: Bicalutamide (BIC) is widely used in prostate cancer therapy. The dose and schedule employed are well tolerated, but about 50% of patients develop gynecomastia. Several studies have shown a significant reduction of the troublesome effects when Tamoxifen is concomitantly administered with BIC. However, the results reported in the literature seem to be preliminary and possible interferences could be present. In order to clarify the molecular mechanisms of the combination of the two drugs, we have investigated whether the expression of the proteins belonging to nuclear matrix (NM), one modulator of hormone action, is altered by BIC and/or 4-hydroxy-tamoxifen (4OHT) in LNCaP cells. We focused above all on heterogeneous nuclear ribonucleoprotein K (hnRNP K) a NM protein with a key role in prostate carcinoma. METHODS: NM proteins were analyzed by two-dimensional gel electrophoresis. Modulation and compartmentalization of the androgen receptor and the hnRNP K were studied by Western blotting, confocal microscopy, and immunoprecipitation. RESULTS: Proteomic analysis revealed that there is a similarity in the changes of the NM proteins elicited by drugs alone but that their combination does not result in a simple additive effect. Moreover, we found that in the nucleoplasm the androgen receptor and the hnRNP K colocalize in a complex that is highly proximal to DNA and that both proteins were synchronously modulated by BIC and/or 4OHT treatment. CONCLUSION: This study confirm the pivotal role of hnRNP K in prostate carcinoma and suggest that this role might be played by the interaction with the androgen receptor.


Assuntos
Antagonistas de Androgênios/farmacologia , Anilidas/farmacologia , Núcleo Celular/química , Antagonistas de Estrogênios/farmacologia , Ribonucleoproteínas Nucleares Heterogêneas Grupo K/análise , Nitrilas/farmacologia , Neoplasias da Próstata/química , Receptores Androgênicos/análise , Tamoxifeno/análogos & derivados , Compostos de Tosil/farmacologia , Linhagem Celular Tumoral , Sobrevivência Celular , Ribonucleoproteínas Nucleares Heterogêneas Grupo K/fisiologia , Humanos , Masculino , Fosforilação , Antígeno Prostático Específico/análise , Neoplasias da Próstata/patologia , Tamoxifeno/farmacologia
10.
Exp Cell Res ; 316(6): 992-1001, 2010 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-20026109

RESUMO

Nuclear lamins are among the more abundant proteins making up the internal nuclear matrix, but very little is known about their structure in the nucleoplasm. Using immunoelectron microscopy, we demonstrate the organization of lamins in the nuclear matrix isolated from rat hepatocytes for the first time. Lamin epitopes are arrayed both in locally ordered clusters and in quasi-regular rows. Fourier filtering of the images demonstrates that the epitopes are placed at the nodes and halfway between the nodes of square or rhombic lattices that are about 50 nm on each side, as well as along rows at regular approximately 25-nm intervals. In addition, we have compared this structure with that of the internal nuclear matrix isolated from persistent hepatocyte nodules. In transformed hepatocytes, the islands of lamin lattice are lost, and only a partial regularity in the rows of gold particles remains. We suggest that orthogonal lattice assembly might be an intrinsic property of lamin molecules, and that the disassembly may be triggered by simple molecular events such as phosphorylation.


Assuntos
Hepatócitos/ultraestrutura , Laminas/metabolismo , Matriz Nuclear/metabolismo , Animais , Células Cultivadas , Epitopos , Hepatócitos/metabolismo , Imuno-Histoquímica , Microscopia Imunoeletrônica , Ratos
11.
Exp Cell Res ; 315(2): 226-39, 2009 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-19000672

RESUMO

Tumor progression is characterized by definite changes in the protein composition of the nuclear matrix (NM). The interactions of chromatin with the NM occur via specific DNA sequences called MARs (matrix attachment regions). In the present study, we applied a proteomic approach along with a Southwestern assay to detect both differentially expressed and MAR-binding NM proteins, in persistent hepatocyte nodules (PHN) in respect with normal hepatocytes (NH). In PHN, the NM undergoes changes both in morphology and in protein composition. We detected over 500 protein spots in each two dimensional map and 44 spots were identified. Twenty-three proteins were differentially expressed; among these, 15 spots were under-expressed and 8 spots were over-expressed in PHN compared to NH. These changes were synchronous with several modifications in both NM morphology and the ability of NM proteins to bind nuclear RNA and/or DNA containing MARs sequences. In PHN, we observed a general decrease in the expression of the basic proteins that bound nuclear RNA and the over-expression of two species of Mw 135 kDa and 81 kDa and pI 6.7-7.0 and 6.2-7.4, respectively, which exclusively bind to MARs. These results suggest that the deregulated expression of these species might be related to large-scale chromatin reorganization observed in the process of carcinogenesis by modulating the interaction between MARs and the scaffold structure.


Assuntos
Neoplasias Hepáticas/metabolismo , Proteínas de Ligação à Região de Interação com a Matriz/metabolismo , Proteínas Associadas à Matriz Nuclear/metabolismo , Matriz Nuclear/metabolismo , Proteômica/métodos , Animais , Western Blotting , Proteínas de Ciclo Celular , Eletroforese em Gel Bidimensional , Proteínas de Choque Térmico/análise , Proteínas de Choque Térmico/metabolismo , Ribonucleoproteínas Nucleares Heterogêneas/análise , Ribonucleoproteínas Nucleares Heterogêneas/metabolismo , Queratinas Tipo II/análise , Queratinas Tipo II/metabolismo , Laminas/análise , Laminas/metabolismo , Neoplasias Hepáticas/patologia , Neoplasias Hepáticas/ultraestrutura , Masculino , Proteínas de Ligação à Região de Interação com a Matriz/análise , Microscopia Eletrônica , Matriz Nuclear/química , Matriz Nuclear/ultraestrutura , Proteínas Associadas à Matriz Nuclear/análise , Proteínas Nucleares/análise , Proteínas Nucleares/metabolismo , Ligação Proteica , RNA Nuclear/metabolismo , Proteínas de Ligação a RNA/análise , Proteínas de Ligação a RNA/metabolismo , Ratos , Ratos Endogâmicos F344 , Ribonucleosídeos/química , Ribonucleosídeos/metabolismo , Espectrometria de Massas em Tandem/métodos , Fatores de Tempo , Vanadatos/química , Vanadatos/metabolismo
12.
Cell Oncol ; 30(1): 13-26, 2008.
Artigo em Inglês | MEDLINE | ID: mdl-18219107

RESUMO

INTRODUCTION: Although several molecular markers for bladder cancer have been identified, at present little information on prognostic biomarkers is available in the literature. Prognostication of this tumor is largely based on clinicopathological characteristics. Our aim was to identify nuclear matrix (NM) proteins that might serve to better characterize the phenotype of the invasive bladder cancer and to investigate their diagnostic and prognostic roles. METHODS: NM proteins expressed in normal (n=3) or non-tumoral (n=9) tissue specimens and muscle-invasive bladder cancer (n=21) specimens were analyzed by two dimensional (2D) gel electrophoresis. PDQuest image analysis software was used to generate a comparative NM proteome analysis. Selected spots were characterized by liquid chromatography coupled to tandem mass spectrometry and Western blot. RESULTS: We detected over 800 protein spots in each 2D map and 43 spots were identified. 30 proteins were differentially expressed by bladder tumor cells; among these, 19 proteins were detected in bladder tumoral tissues but not in normal and non-tumoral tissues and seven proteins correlated with tumor stage. One protein (p54nrb) was strongly correlated with vascular invasions and appeared to be also significantly (P<0.0001) associated with a decreased probability of survival. CONCLUSION: Important alterations in NM proteins occur in muscle-invasive bladder cancer. The differentially expressed proteins include biomarkers potentially useful for disease diagnosis, progression and prognosis. Our findings beyond improving the understanding of the biology of bladder cancer, could help to stratify patients into different prognostic subgroups and to select those who might be better candidate to multimodal therapeutic approaches.


Assuntos
Proteínas Associadas à Matriz Nuclear/análise , Proteoma/análise , Neoplasias da Bexiga Urinária/química , Idoso , Idoso de 80 Anos ou mais , Biomarcadores Tumorais/análise , Western Blotting , Eletroforese em Gel Bidimensional , Feminino , Humanos , Estimativa de Kaplan-Meier , Masculino , Pessoa de Meia-Idade , Proteínas de Neoplasias/análise , Prognóstico , Proteômica , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Neoplasias da Bexiga Urinária/diagnóstico , Neoplasias da Bexiga Urinária/patologia
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