RESUMO
This study evaluated the effectiveness of the essential oil of Pimenta pseudocaryophyllus in inhibiting the growth of the main bacteria responsible for bad perspiration odor (Staphylococcus epidermidis, Proteus hauseri, Micrococcus yunnanensis and Corynebacterium xerosis). The chemical profile of the essential oil was evaluated by high-resolution gas chromatography (HR-GC) and four constituents were identified, eugenol being the major component (88.6%). The antimicrobial activity was evaluated by means of the turbidimetric method, using the microdilution assay. The minimum inhibitory concentration (MIC) values of the essential oil ranged from 500 to 1,000 µg mL⻹. Scanning electron microscope (SEM) observations confirmed the physical damage and morphological alteration of the test bacteria treated with the essential oil, reference drugs and eugenol. The findings of the study demonstrated that this essential oil can be used in the formulation of personal care products.
Assuntos
Anti-Infecciosos/farmacologia , Óleos Voláteis/farmacologia , Pimenta , Óleos de Plantas/farmacologia , Anti-Infecciosos/isolamento & purificação , Bactérias Aeróbias Gram-Negativas/efeitos dos fármacos , Bactérias Aeróbias Gram-Negativas/crescimento & desenvolvimento , Bactérias Aeróbias Gram-Negativas/ultraestrutura , Bactérias Gram-Positivas/efeitos dos fármacos , Bactérias Gram-Positivas/crescimento & desenvolvimento , Bactérias Gram-Positivas/ultraestrutura , Testes de Sensibilidade Microbiana , Microscopia Eletrônica de Varredura , Óleos Voláteis/isolamento & purificação , Fitoterapia , Pimenta/química , Óleos de Plantas/isolamento & purificação , Plantas MedicinaisRESUMO
El uso de dientes naturales en el proceso de enseñanza-aprendizaje de Odontología es fundamental, además los nuevos planes curriculares vigentes en la mayoría de instituciones de enseñanza brasileras de nivel superior dan privilegio a la investigación. Es de fundamental relevancia conocer tanto la procedencia y formas de uso de los dientes humanos extraídos, como los procedimientos de descontaminación y almacenamiento empleados para este fin. El presente trabajo tiene como objetivo analizar la forma de uso de dientes humanos en la enseñanza odontológica en la Facultad de Odontología de la Universidad Federal de Juiz de Fora a partir de la aplicación de 200 cuestionarios a los alumnos de primero a séptimo periodo de formación. Los resultados demuestran que todavía existe una despreocupación en lo que se refiere a los aspectos ético-legales y de bioseguridad en la obtención y manipulación de dientes humanos por parte de los alumnos. Los datos demuestran la relevancia de la funcionalidad del Banco de Dientes Humanos encargada de abastecer de dientes a los académicos, profesores e investigadores, de forma ética y legal dentro de los estándares de bioseguridad, favoreciendo al menor riesgo de contaminación cruzada.
The use of natural teeth in the dental education and research is essential, moreover, the new curriculum model currently in the majority of Brazilian institutions of higher education focuses on research practice. Besides the importance of knowing the origin and ways of using extracted human teeth, another relevant question is whether the procedures for decontamination and storage are being employed. This study aimed to investigate the use of human teeth in dental education in College of Dentistry of Federal University of Juiz de Fora by application of 200 questionnaires to students between 10 and 70 periods. Given the above study, it appears that there is still a lack of concern with the ethical-legal and bio-security in obtaining and handling human teeth by students. Data show the relevance of the functionality of the Human Teeth Bank, with the role of providing teeth to academics, teachers and researchers in an ethical, legal and standards of biosecurity, providing less risk of cross contamination.
