From Nature to Lab: A Review of Secondary Metabolite Biosynthetic Pathways, Environmental Influences, and In Vitro Approaches.

Secondary metabolites are gaining an increasing importance in various industries, such as pharmaceuticals, dyes, and food, as is the need for reliable and efficient methods of procuring these compounds. To develop sustainable and cost-effective approaches, a comprehensive understanding of the biosynthetic pathways and the factors influencing secondary metabolite production is essential. These compounds are a unique type of natural product which recognizes the oxidative damage caused by stresses, thereby activating the defence mechanism in plants. Various methods have been developed to enhance the production of secondary metabolites in plants. The elicitor-induced in vitro culture technique is considered an efficient tool for studying and improving the production of secondary metabolites in plants. In the present review, we have documented various biosynthetic pathways and the role of secondary metabolites under diverse environmental stresses. Furthermore, a practical strategy for obtaining consistent and abundant secondary metabolite production via various elicitation agents used in culturing techniques is also mentioned. By elucidating the intricate interplay of regulatory factors, this review paves the way for future advancements in sustainable and efficient production methods for high-value secondary metabolites.

Comparative effects of caffeine and lead nitrate on the bio-physiological and yield associated traits of lentil (Lens culinaris Medik.).

Lentil belonging to the fabaceae family is a proteinaceous cool-season legume consumed across the world. However, lentil is low yielding with a narrow genetic base compared to other grain legumes such as chickpea, faba bean, and cowpea. In the present study, we intended to investigate the effect of two different mutagens viz., caffeine and lead nitrate on the bio-physiological and agronomical traits of lentil. Unlike other mutagens like ethyl methanesulphonate, sodium azide, and hydrazine hydrates very little is known about the mutagenic potency of caffeine and lead nitrate. The results revealed contrasting effects as lower doses of caffeine-induced a substantial increase in mean values of physiological and agronomical traits whereas both lower and higher doses of lead nitrate negatively impacted the agronomical traits of lentil. Among the mutagen doses, 0.1% caffeine was most efficient in inducing a substantial increase in mean values of bio-physiological and quantitative traits. The present study also revealed the successful conduct of induced mutagenesis in lentil and present a protocol that could be followed in future breeding programs aimed at increasing the yielding potential of legumes.