Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Appl Microbiol Biotechnol ; 104(1): 405-415, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31832709

RESUMO

Recently Legionella pneumophila is the main causative waterborne organism of severe respiratory infections. Additionally, other Legionella species are documented as human pathogens. In our work, we describe a rapid detection method which combines two advantages for sensitive and specific detection of the genus Legionella: the fast isothermal amplification method "Loop-mediated isothermal AMPlification" (LAMP), and a colorimetric detection method using the metal indicator hydroxynaphtol blue (HBN) which allows to determine an optical signal with a simple readout (with the naked eye). Moreover, we present two approaches for minimizing the assay volume using a stationary microchip LAMP and droplet digital-based LAMP (ddLAMP) as promising highly sensitive setups.


Assuntos
Legionella pneumophila/isolamento & purificação , Legionella/isolamento & purificação , Técnicas de Amplificação de Ácido Nucleico/métodos , Colorimetria , Primers do DNA/genética , Naftalenossulfonatos/metabolismo , Sensibilidade e Especificidade , Temperatura
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...