The impact of nTiO2 and GO (graphene oxide), and their combinations, on freshwater Chlorella sp.: a comparative study in lake water and BG-11 media.

Titanium dioxide nanoparticles (nTiO2) and graphene oxide (GO) are extensively used nanomaterials in various products and applications. Freshwater ecosystems are a crucial sink for these pollutants, posing severe threats to aquatic organisms. Although multiple studies have investigated the pristine toxicity of nTiO2 and GO in freshwater organisms, the combined toxicity of these materials remains unexplored. Interaction media is a crucial factor in evaluating toxicity nanomaterial toxicity towards algae. In this study, we have investigated the comparative effect of sterilized and filtered freshwater and BG-11 medium on the pristine and combined toxicity of nTiO2 and GO on freshwater algae Chlorella sp. Results indicated that the combination of nTiO2 and GO showed more toxicity when compared to their respective pristine forms. This could be due to the additive effect exhibited by nTiO2 and GO on Chlorella sp. The enhanced growth inhibition for the combined toxicity was in the order of 1 mg L-1 nTiO2 + 1 mg L-1 GO > 1 mg L-1 nTiO2 + 0.1 mg L-1 GO > 0.1 mg L-1 nTiO2 + 1 mg L-1 GO > 0.1 mg L-1 nTiO2 + 0.1 mg L-1 GO. All test groups that interacted in BG-11 media exhibited less toxicity when compared to corresponding groups in the lake water medium. This could be attributed to the cushioning effect of BG-11 medium, providing supplementary nutrition to the algal cells. This signifies that the environmentally relevant conditions could be more detrimental than the laboratory conditions. This study elucidates valuable insights into the potential detrimental effects associated with the combination of nTiO2 and GO on freshwater algae. Furthermore, we have evaluated the growth inhibition, oxidative stress, and photosynthetic activity of Chlorella sp. in both environmentally relevant interaction medium and well-defined culture medium.

The comparative effects of visible light and UV-A radiation on the combined toxicity of P25 TiO2 nanoparticles and polystyrene microplastics on Chlorella sp.

The ubiquitous presence of TiO2 nanoparticles (nTiO2) and microplastics (MPs) in marine ecosystems has raised serious concerns about their combined impact on marine biota. This study investigated the combined toxic effect of nTiO2 (1 mg/L) and NH2 and COOH surface functionalized polystyrene MPs (PSMPs) (2.5 and 10 mg/L) on Chlorella sp. All the experiments were carried out under both visible light and UV-A radiation conditions to elucidate the impact of light on the combined toxicity of these pollutants. Growth inhibition results indicated that pristine nTiO2 exhibited a more toxic effect (38%) under UV-A radiation when compared to visible light conditions (27%). However, no significant change in the growth inhibitory effects of pristine PSMPs was observed between visible light and UVA radiation conditions. The combined pollutants (nTiO2 + 10 mg/L PSMPs) under UV-A radiation exhibited more growth inhibition (nTiO2 + NH2 PSMPs 66%; nTiO2 + COOH PSMPs 50%) than under visible light conditions (nTiO2 + NH2 PSMPs 55%; TiO2 + COOH PSMPs 44%). Independent action modeling indicated that the mixture of nTiO2 with PSMPs (10 mg/L) exhibited an additive effect on the algal growth inhibition under both the light conditions. The photoactive nTiO2 promoted increased production of reactive oxygen species under UV-A exposure, resulting in cellular damage, lipid peroxidation, and impaired photosynthesis. The effects were more pronounced in case of the mixtures where PSMPs added to the oxidative stress. The toxic effects of the binary mixtures of nTiO2 and PSMPs were further confirmed through the field emission electron microscopy, revealing specific morphological abnormalities. This study provides valuable insights into the potential risks associated with the combination of nTiO2 and MPs in marine environments, considering the influence of environmentally relevant light conditions and the test medium.

Ecotoxicological significance of bio-corona formation on micro/nanoplastics in aquatic organisms.

The unsustainable manufacturing, utilization and inadequate handling of plastics have led to a surge in global plastic pollution. In recent times, there has been increasing concern about the plausible hazards associated with exposure to micro/nanoplastics (M/NPs). As aquatic systems are considered to be the likely sink for M/NPs, it is crucial to comprehend their environmental behavior. The bioavailability, toxicity and fate of M/NPs in the environment are predominantly dictated by their surface characteristics. In the aquatic environment, M/NPs are prone to be internalized by aquatic organisms. This may facilitate their interaction with a diverse array of biomolecules within the organism, resulting in the formation of a biocorona (BC). The development of BC causes modifications in the physicochemical attributes of the M/NPs including changes to their size, stability, surface charge and other properties. This review details the concept of BC formation and its underlying mechanism. It provides insight on the analytical techniques employed for characterizing BC formation and addresses the associated challenges. Further, the eco-toxicological implications of M/NPs and the role of BC in modifying their potential toxicity on aquatic organisms is specified. The impact of BC formation on the fate and transport of M/NPs is discussed. A concise outlook on the future perspectives is also presented.