RESUMO
The control of gynecium development in Arabidopsis thaliana by the auxin response factor ETTIN (ETT) correlates with a reduction in the methylesterification of cell-wall pectins and a decrease in cell-wall stiffness in the valve tissues of the ovary. Here, we determine the list of genes rapidly regulated following the in-vivo activation of an ETT fusion protein, and show these to be significantly enriched in genes encoding cell-wall proteins, including several pectin methylesterases (PMEs) and pectin methylesterase inhibitors (PMEIs). We also perform a genome-wide scan for potential ETT-binding sites, and incorporate the results of this procedure into a comparison of datasets, derived using four distinct methods, to identify genes regulated directly or indirectly by ETT. We conclude from our combined analyses that PMEIs are likely to be key actors that mediate the regulation of gynecium development by ETT, while ETT may simultaneously regulate PMEs to prevent exaggerated developmental effects from the regulation of PMEIs. We also postulate the existence of one or more rapidly-acting intermediate factors in the transcriptional regulation of PMEs and PMEIs by ETT.
Assuntos
Proteínas de Arabidopsis/metabolismo , Parede Celular/metabolismo , Proteínas de Ligação a DNA/metabolismo , Arabidopsis/genética , Arabidopsis/metabolismo , Proteínas de Arabidopsis/genética , Proteínas de Ligação a DNA/genética , Regulação da Expressão Gênica de Plantas/genética , Regulação da Expressão Gênica de Plantas/fisiologia , Pectinas/metabolismo , Proteínas de Plantas/metabolismoRESUMO
ETTIN (ETT) is an atypical member of the AUXIN RESPONSE FACTOR family of transcription factors that plays a crucial role in tissue patterning in the Arabidopsis (Arabidopsis thaliana) gynoecium. Though recent insights have provided valuable information on ETT's interactions with other components of auxin signaling, the biophysical mechanisms linking ETT to its ultimate effects on gynoecium morphology were until now unknown. Here, using techniques to assess cell-wall dynamics during gynoecium growth and development, we provide a coherent body of evidence to support a model in which ETT controls the elongation of the valve tissues of the gynoecium through the positive regulation of pectin methylesterase (PME) activity in the cell wall. This increase in PME activity results in an increase in the level of demethylesterified pectins and a consequent reduction in cell wall stiffness, leading to elongation of the valves. Though similar biophysical mechanisms have been shown to act in the stem apical meristem, leading to the expansion of organ primordia, our findings demonstrate that regulation of cell wall stiffness through the covalent modification of pectin also contributes to tissue patterning within a developing plant organ.
Assuntos
Proteínas de Arabidopsis/metabolismo , Arabidopsis/genética , Hidrolases de Éster Carboxílico/metabolismo , Proteínas de Ligação a DNA/metabolismo , Regulação da Expressão Gênica de Plantas , Proteínas Nucleares/metabolismo , Pectinas/metabolismo , Arabidopsis/crescimento & desenvolvimento , Arabidopsis/metabolismo , Proteínas de Arabidopsis/genética , Hidrolases de Éster Carboxílico/genética , Parede Celular/enzimologia , Proteínas de Ligação a DNA/genética , Flores/genética , Flores/crescimento & desenvolvimento , Flores/metabolismo , Regulação da Expressão Gênica no Desenvolvimento , Meristema/genética , Meristema/crescimento & desenvolvimento , Meristema/metabolismo , Proteínas Nucleares/genética , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismoRESUMO
A key innovation of flowering plants is the female reproductive organ, the carpel. Here, we show that a mechanism that regulates carpel margin development in the model flowering plant Arabidopsis thaliana was recruited from light-regulated processes. This recruitment followed the loss from the basic helix-loop-helix transcription factor SPATULA (SPT) of a domain previously responsible for its negative regulation by phytochrome. We propose that the loss of this domain was a prerequisite for the light-independent expression in female reproductive tissues of a genetic module that also promotes shade avoidance responses in vegetative organs. Striking evidence for this proposition is provided by the restoration of wild-type carpel development to spt mutants by low red/far-red light ratios, simulating vegetation shade, which we show to occur via phytochrome B, PHYTOCHROME INTERACTING FACTOR4 (PIF4), and PIF5. Our data illustrate the potential of modular evolutionary events to generate rapid morphological change and thereby provide a molecular basis for neo-Darwinian theories that describe this nongradualist phenomenon. Furthermore, the effects shown here of light quality perception on carpel development lead us to speculate on the potential role of light-regulated mechanisms in plant organs that, like the carpel, form within the shade of surrounding tissues.
Assuntos
Proteínas de Arabidopsis/genética , Arabidopsis/genética , Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética , Flores/genética , Regulação da Expressão Gênica no Desenvolvimento/genética , Sequência de Aminoácidos , Arabidopsis/citologia , Arabidopsis/crescimento & desenvolvimento , Arabidopsis/efeitos da radiação , Proteínas de Arabidopsis/metabolismo , Sequência de Bases , Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo , Evolução Molecular , Flores/citologia , Flores/crescimento & desenvolvimento , Flores/efeitos da radiação , Regulação da Expressão Gênica de Plantas/genética , Luz , Modelos Moleculares , Dados de Sequência Molecular , Mutação , Motivos de Nucleotídeos/genética , Fenótipo , Filogenia , Fitocromo B/genética , Plantas Geneticamente Modificadas , Regiões Promotoras Genéticas , Estrutura Terciária de Proteína/genética , Análise de Sequência de DNA , TranscriptomaRESUMO
We demonstrate that the biophysical technique of surface plasmon resonance (SPR) analysis, which has previously been used to measure transcription factor binding to short DNA molecules, can also be used to characterize interactions involving entire gene promoters. This discovery has two main implications that relate, respectively, to novel qualitative and quantitative uses of the SPR technique. Firstly, SPR analysis can be used qualitatively to test the capacity of any transcription factor to interact physically with its putative target genes. This application should prove particularly useful for the confirmation of predicted transcriptional interactions in model species, and for comparative studies of non-model species in which transcriptional interactions are not amenable to study by other methods. Secondly, SPR may be used quantitatively to characterize interactions between transcription factors and gene promoters containing multiple cis-acting sites. This application should prove useful for the detailed dissection of promoter function in known target genes. The qualitative and quantitative applications of the SPR analysis of whole promoters combine to make this a uniquely powerful technique, which should prove particularly useful in systems biology, evolutionary developmental biology and various branches of applied biology.
