Unveiling Fungal Community Structure along Different Levels of Anthropic Disturbance in a South American Subtropical Lagoon.

Studies of fungal communities through amplicon metagenomics in aquatic environments, particularly in freshwater ecosystems, are still relatively recent. Unfortunately, many of these water bodies are facing growing threats from human expansion, such as effluent discharge from various human activities. As a result, these effluents have the potential to significantly alter the characteristics of water bodies and, subsequently, impact the diversity of their resident microorganisms. In this context, our objective was to investigate whether the fungal community structure varies according to the presence of different anthropic disturbances. We expect (i) the diversity of fungi will be greater and (ii) more specific unique operational taxonomic units (OTUs) related to each ecotonal system will be found compared to other sites of a lagoon. The study was conducted in the Tramandaí Lagoon (subtropical southern Brazil) at four distinct sampling points (estuary, middle of the lagoon, crop field area, and near a residential area where the Tramandaí River flows into the lagoon). As expected, the estuary and residential zones, which are ecotones, exhibited greater fungal diversity and more specific OTUs compared to the middle of the lagoon and crop field area. Moreover, a substantial proportion of fungal taxa could not be identified at the genus level, with many only classified at the phylum level, indicating potential new lineages. These findings underscore our limited understanding of the subtropical freshwater mycobiota.

Disentangling the Taxonomy, Systematics, and Life History of the Spider-Parasitic Fungus Gibellula (Cordycipitaceae, Hypocreales).

Gibellula (Cordycipitaceae, Hypocreales) is frequently observed growing on spiders, but little is known about their host range. One of the greatest challenges in describing these interactions is identifying the host, since the fungus often rapidly consumes the parasitised spiders and destroys important diagnostic taxonomic traits. Additionally, the global diversity of Gibellula remains unclear, as does the natural history and phylogenetic relationships of most of the species. Herein, we performed an extensive investigation on the species of Gibellula, reconstructed the most complete molecular phylogeny of the genus in the context of Cordycipitaceae, and performed a systematic review in order to provide the foundations towards a better understanding of the genus. Therefore, we have performed an integrative study to investigate the life history of the genus and to disentangle the questionable number of valid species proposed over time. We provided novel molecular data for published species that had not been sequenced before, such as G. mirabilis and G. mainsii, and evaluated all the original and modern morphological descriptions. In addition, we presented its global known distribution and compiled all available molecular data. We suggested a set of terms and morphological traits that should be considered in future descriptions of the genus and that a total of 31 species should be considered as accepted.

Synchronous jawbone diseases: a multicenter retrospective study.

The aim of this study is to report an original case series of synchronous jawbone diseases. Data of patients seen over 13 years were extracted from the files of three Oral Radiology and Pathology diagnostic centers in Brazil. The clinical, radiographic, and laboratory characteristics were tabulated and analyzed by the authors; the patients were described according to lesion type. Seventy-two synchronous jawbone diseases were included in this study. Florid osseous dysplasia, Gorlin-Goltz syndrome, and cherubism were the most frequent disorders reported in this case series. In addition, the posterior mandible area was the main site of manifestation. Florid osseous dysplasia and Gorlin-Goltz syndrome represented two-thirds of our samples. With the utilization of adequate demographic, clinical, and radiologic information, it is possible to diagnose most of the synchronous lesions of jawbones. Sometimes, however, we need complementary exams, such as histopathologic and biochemical analysis or dosing of calcium, phosphorus, and alkaline phosphatase.

Synchronous jawbone diseases: a multicenter retrospective study

Abstract The aim of this study is to report an original case series of synchronous jawbone diseases. Data of patients seen over 13 years were extracted from the files of three Oral Radiology and Pathology diagnostic centers in Brazil. The clinical, radiographic, and laboratory characteristics were tabulated and analyzed by the authors; the patients were described according to lesion type. Seventy-two synchronous jawbone diseases were included in this study. Florid osseous dysplasia, Gorlin-Goltz syndrome, and cherubism were the most frequent disorders reported in this case series. In addition, the posterior mandible area was the main site of manifestation. Florid osseous dysplasia and Gorlin-Goltz syndrome represented two-thirds of our samples. With the utilization of adequate demographic, clinical, and radiologic information, it is possible to diagnose most of the synchronous lesions of jawbones. Sometimes, however, we need complementary exams, such as histopathologic and biochemical analysis or dosing of calcium, phosphorus, and alkaline phosphatase.

Long-term unsustainable patterns of development rather than recent deforestation caused the emergence of Orthocoronavirinae species.

We investigated whether a set of phylogeographical tracked emergent events of Orthocoronavirinae were related to developed, urban and polluted environments worldwide. We explored coronavirus records in response to climate (rainfall parameters), population density, CO2 emission, Human Developmental Index (HDI) and deforestation. We contrasted environmental characteristics from regions with spillovers or encounters of wild Orthocoronavirinae against adjacent areas having best-preserved conditions. We used all complete sequenced CoVs genomes deposited in NCBI and GISAID databases until January 2021. Except for Deltacoronavirus, concentrated in Hong Kong and in birds, the other three genera were scattered all over the planet, beyond the original distribution of the subfamily, and found in humans, mammals, fishes and birds, wild or domestic. Spillovers and presence in wild animals were only reported in developed/densely populated places. We found significantly more occurrences reported in places with higher HDI, CO2 emission, or population density, along with more rainfall and more accentuated seasonality. Orthocoronavirinae occurred in areas with significantly higher human populations, CO2 emissions and deforestation rates than in adjacent locations. Intermediately disturbed ecosystems seemed more vulnerable for Orthocoronavirinae emergence than forested regions in frontiers of deforestation. Sadly, people experiencing poverty in an intensely consumerist society are the most vulnerable.

An Integrative View of the Phyllosphere Mycobiome of Native Rubber Trees in the Brazilian Amazon.

The rubber tree, Hevea brasiliensis, is a neotropical Amazonian species. Despite its high economic value and fungi associated with native individuals, in its original area in Brazil, it has been scarcely investigated and only using culture-dependent methods. Herein, we integrated in silico approaches with novel field/experimental approaches and a case study of shotgun metagenomics and small RNA metatranscriptomics of an adult individual. Scientific literature, host fungus, and DNA databases are biased to fungal taxa, and are mainly related to rubber tree diseases and in non-native ecosystems. Metabarcoding retrieved specific phyllospheric core fungal communities of all individuals, adults, plantlets, and leaves of the same plant, unravelling hierarchical structured core mycobiomes. Basidiomycotan yeast-like fungi that display the potential to produce antifungal compounds and a complex of non-invasive ectophytic parasites (Sooty Blotch and Flyspeck fungi) co-occurred in all samples, encompassing the strictest core mycobiome. The case study of the same adult tree (previously studied using culture-dependent approach) analyzed by amplicon, shotgun metagenomics, and small RNA transcriptomics revealed a high relative abundance of insect parasite-pathogens, anaerobic fungi and a high expression of Trichoderma (a fungal genus long reported as dominant in healthy wild rubber trees), respectively. Altogether, our study unravels new and intriguing information/hypotheses of the foliar mycobiome of native H. brasiliensis, which may also occur in other native Amazonian trees.

Liposarcoma of oral cavity: Systematic review of cases reported to date and analysis of prognostic factors.

The aim of this study was to integrate the available data published on Liposarcomas (LPSs) of the oral cavity into an analysis of its clinical features, treatment modalities, and prognostic factors. An electronic search was undertaken in January 2019. The eligibility criteria included publications that had enough clinical and histological information to confirm the diagnosis. Forty-five publications (104 LPSs) were included. The lesion was more prevalent in males from the fifth to seventh decades of life. Treatment (P = .03) and distant metastasis (P = .0001) were independently associated with survival. A lower possibility of recurrence was statistically associated with age (younger patients) (P = .03), tumor size (smaller than 2.8 cm) (P = .001), and treatment (radical surgery) (P = .04). LPS presents a good survival for patients after 5 years of follow-up (66.4%). Patients who were treated with conservative surgery and presented with distant metastasis showed poor prognosis.

Proteomic fingerprinting for the fast and accurate identification of species in the Polyporoid and Hymenochaetoid fungi clades.

Basidiomycotan fungi play significant roles in the biogeochemical cycle of carbon as wood decomposers and are used in the food industry for mushroom production and in biotechnology for the production of diverse bioactive compounds and bioremediation. The correct identification of basidiomycotan isolates is crucial for understanding their biology and being able to expand their applications. Currently, the identification of these organisms is performed by analyzing morphological and genomic characteristics, primarily those based on DNA biomarkers. Despite their efficiency, such methods require considerable expertise and are both time-consuming and error-prone (multistep workflow). Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has emerged in the last decade as an accurate, fast, and powerful alternative for the identification of microorganisms. MALDI-TOF MS has been widely applied for the identification and taxonomical characterization of both bacteria and ascomycotan fungi from clinical origins. However, species of Basidiomycota have been poorly evaluated using this method. In the present study, we assessed the performance of MALDI-TOF MS using basidiomycotan isolates of two distinct taxonomical families: Polyporaceae and Hymenochaetaceae. Using a simple protocol, which eliminates the protein extraction step, we obtained high-quality mass spectra data and demonstrated that this method is efficient for the discrimination of isolates at the species level. SIGNIFICANCE: In this study, the MALDI-TOF mass spectrometry was employed to test its accuracy on the recognition of fungal species with high biotechnological and environmental interest. Using a simple and fast protocol, we obtained high-quality mass-spectra (protein fingerprinting) and proved that MALDI-TOF MS is sufficiently robust to the identification at species level and to evaluate the relationships among the isolates of the polyporoid and hymenochaetoid clades (Basidiomycota).

Melanotic neuroectodermal tumor of infancy of the jaw bones: Update on the factors influencing survival and recurrence.

The aim of the present study was to integrate the available data published on melanotic neuroectodermal tumor of infancy (MNTI) of the jaw bones. An electronic search was undertaken in April 2018. Hundred forty-seven publications and 371 patients were included. The lesion was more prevalent in males and in the second to sixth months of life. The lesions mostly presented as a rapidly growing bluish swelling and the most commonly involved was the anterior maxilla. The mean follow-up was 51.1 months (range 1-408 months). Age (P ≤ .0001), location (P = .007), occurrence of lymph node metastasis (P ≤ .0001), treatment (P = .001), recurrence (P ≤ .0001), and distant metastasis (P = .0001) were independently associated with survival. Recurrence was significantly correlated with age (P = .0001), distant metastasis (P = .0001), and treatment (P = .0001). Patients older than 12 months, with lesions in the mandible, positive regional lymph node metastasis, treated with chemotherapy, recurrence, or distant metastasis presented the worst prognosis.

Oral and maxillofacial manifestations of chronic kidney disease-mineral and bone disorder: a multicenter retrospective study.

OBJECTIVE: To describe the oral and maxillofacial manifestations of patients diagnosed with chronic kidney disease-mineral and bone disorders. STUDY DESIGN: Over a 13-year period, clinicopathologic data of patients diagnosed with CKD-MBD who had oral and maxillofacial alterations were retrieved from the files of 4 Brazilian institutions. Data included clinical, radiographic, microscopic, and biochemical findings; treatment employed; and follow-up status. RESULTS: Twenty-one cases were identified, with 13 patients diagnosed as brown tumor of hyperparathyroidism (BTH) and 8 as osteitis fibrosa/renal osteodystrophy (OF/RO) (4 of them clinically consistent with Sagliker syndrome). The mean age was 32.7 years, and the mandible was the most affected site (42.8%). OF/RO had an ill-defined "ground glass" radiographic appearance, and BTH produced well-defined radiolucent images. Biochemically the following mean values were obtained: parathormone 1511.07 pg/mL, calcium 9.25 mg/dL, phosphorus 5.19 mg/dL, alkaline phosphatase 941.55 U/L, urea 125.42 mg/dL, and creatinine 7.14 mg/dL. Treatment comprised vitamin D and calcium intake, parathyroidectomy, hemodialysis, renal transplantation, and local surgery. During follow-up, 5 patients with BTH were free of lesions, whereas 2 affected by OF/RO/Sagliker syndrome died. CONCLUSIONS: Oral and maxillofacial manifestations of BTH and OF/RO are uncommon, but they can be associated with an important life-threatening scenario.

Performers sem Fronteiras, uma plataforma clínico-performativa de ações em arte relacional / Performers without borders, a clinical-performative platform of actions in relational art

Resumo O artigo aborda o trabalho da plataforma clínico-performativa Performers sem Fronteiras (PsF), que trabalha em contextos de pessoas em situação de trauma pontual ou crônico, bem como em ações de cultivo e promoção de saúde. Vamos abordar a interface arte/cura a partir do conceito de PARC (Performances de Arte Relacional como prática de Cura), desenvolvido por Tania Alice, em algumas ações clínico-performáticas realizadas pelo conjunto da plataforma, em volta de três eixos norteadores do trabalho do coletivo: 1. O movimento livre como prática clínica pela criação de plataformas pontuais de livre expressão do corpo. 2. O empoderamento dos participantes, pensado a partir de um trabalho realizado por dois integrantes do PsF junto a usuários da rede de saúde mental do Rio de Janeiro intitulado "Todo sonho bem sonhado pode um dia virar realidade". 3. A potencialização dos afetos dentro da performance "Correio de abraços Brasil/Nepal" de Tania Alice.(AU)

Abstract The article discusses the work of the clinical-performative platform Performers without Borders (PsF), which works in contexts of people with punctual or chronic trauma, as well as in actions that cultivate and promote health. We will aproach the art/healing interface based on the concept of PARC (Performances of Relational Art as Cure practice), developed by Tania Alice, in some clinical-performative actions performed by the platform, set around three axes guiding the work of the collective: 1. The free movement as therapeutic practice by the creation of punctual platforms of free expression of the body. 2. The empowerment of the participants, based on work done by two members of the PsF together with users of the mental health network in Rio de Janeiro entitled "Every dream well dreamed can one day become a reality". 3. The potentialization of the affections within the performance "The hug Project Brazil/Nepal" of Tania Alice. (AU)

Anatomical variation of mandibular canal simulating a recurrence of odontogenic tumor.

Mandibular nerve has an important role in the field of oral maxillofacial surgery. Furthermore, several anatomical variations can be found and are clinically relevant mainly in procedures involving the posterior mandible. The unknown of these anatomical variations of the inferior alveolar nerve have been implicated with complications in the performance of surgical procedures and anesthesia in dental and maxillofacial practice. The present paper reports a rare anatomical variation of inferior alveolar nerve mimicking a recurrence of keratocystic odontogenic tumor.

Estágio em psicologia escolar e arte: contribuições para a formação do Psicólogo / Internship in school psychology and art: contributions to the professional formation of the psychologist / Pasantía en psicología escolar y arte: contribuciones para la formación del Psicólogo

O presente trabalho relata as experiências vivenciadas em um estágio de Psicologia escolar realizado no Curso de Psicologia da Universidade Federal de Uberlândia (UFU). O estágio, pautado na Psicologia histórico-cultural, estava vinculado à organização e ao desenvolvimento de uma disciplina intitulada Psicologia: Ciência e Profissão, voltada para os alunos que ingressavam no referido curso. A ementa dessa disciplina vai ao encontro da necessidade de desenvolvimento de uma visão crítica e socialmente comprometida em relação à Psicologia, o que permite a constituição de um espaço de possibilidades de se considerar essa ciência e profissão de um modo mais amplo. As atividades desenvolvidas nos encontros buscavam instigar reflexões acerca da Universidade, da Psicologia como ciência e profissão e da vida acadêmica. Para embasar essas discussões, a Arte (cinema, pintura, teatro, literatura, música) foi utilizada como mote para a apropriação e a construção do próprio conhecimento. Descrevemos aqui a trajetória desse estágio e as suas repercussões na formação pessoal e profissional dos futuros psicólogos, dando destaque para as contribuições desencadeadas a partir do contato com a Arte tanto para os estagiários como para a supervisora.(AU)

This paper describes the experience acquired during a school psychology internship in the psychology course in the Federal University of Uberlândia (UFU). The internship, based on the culturalhistorical psychology, was linked to the organization and development of a course titled Psychology: Science and Profession, which is oriented towards freshmen students. The program of this course meets the need to develop a socially involved and critical vision of psychology, which allows the creation of a space of possibilities to think about this science and profession more broadly. The activities developed in the meetings sought to engage reflections about the University, about psychology as a science and profession and about the academic life. To serve as base for discussion, Art (cinema, painting, theatre, literature, music) was used as a theme for ownership and construction of self knowledge. We describe the results of the internship and its impact on the personal and professional education of future psychologists, highlighting the contributions triggered by the contact with Art.(AU)

El presente trabajo relata las experiencias vividas en una pasantía de Psicología escolar realizada en el Curso de Psicología de la Universidad Federal de Uberlândia (UFU). La pasantía, pautada en la Psicología histórico-cultural, estaba vinculada a la organización y al desarrollo de una disciplina titulada Psicología: Ciencia y Profesión, volcada a los alumnos que ingresaban en el referido curso. El contenido programático de esa disciplina va al encuentro de la necesidad de desarrollo de una visión crítica y socialmente comprometida con relación a la Psicología, lo que permite la constitución de un espacio de posibilidades de considerarse esa ciencia y profesión de un modo más amplio. Las actividades desarrolladas en los encuentros buscaban incitar reflexiones acerca de la Universidad, de la Psicología como ciencia y profesión y de la vida académica. Para la base esas discusiones, el Arte (cine, pintura, teatro, literatura, música) fue utilizado como recurso para la apropiación y la construcción del propio conocimiento. Describimos aquí la trayectoria de esa pasantía y sus repercusiones en la formación personal y profesional de los futuros psicólogos, dando destaque para las contribuciones desencadenadas desde el contacto con el Arte tanto para los practicantes como para la supervisora.(AU)

Estágio em psicologia escolar e arte: contribuições para a formação do Psicólogo / Internship in school psychology and art: contributions to the professional formation of the psychologist / Pasantía en psicología escolar y arte: contribuciones para la formación del Psicólogo

O presente trabalho relata as experiências vivenciadas em um estágio de Psicologia escolar realizado no Curso de Psicologia da Universidade Federal de Uberlândia (UFU). O estágio, pautado na Psicologia histórico-cultural, estava vinculado à organização e ao desenvolvimento de uma disciplina intitulada Psicologia: Ciência e Profissão, voltada para os alunos que ingressavam no referido curso. A ementa dessa disciplina vai ao encontro da necessidade de desenvolvimento de uma visão crítica e socialmente comprometida em relação à Psicologia, o que permite a constituição de um espaço de possibilidades de se considerar essa ciência e profissão de um modo mais amplo. As atividades desenvolvidas nos encontros buscavam instigar reflexões acerca da Universidade, da Psicologia como ciência e profissão e da vida acadêmica. Para embasar essas discussões, a Arte (cinema, pintura, teatro, literatura, música) foi utilizada como mote para a apropriação e a construção do próprio conhecimento. Descrevemos aqui a trajetória desse estágio e as suas repercussões na formação pessoal e profissional dos futuros psicólogos, dando destaque para as contribuições desencadeadas a partir do contato com a Arte tanto para os estagiários como para a supervisora...

This paper describes the experience acquired during a school psychology internship in the psychology course in the Federal University of Uberlândia (UFU). The internship, based on the culturalhistorical psychology, was linked to the organization and development of a course titled Psychology: Science and Profession, which is oriented towards freshmen students. The program of this course meets the need to develop a socially involved and critical vision of psychology, which allows the creation of a space of possibilities to think about this science and profession more broadly. The activities developed in the meetings sought to engage reflections about the University, about psychology as a science and profession and about the academic life. To serve as base for discussion, Art (cinema, painting, theatre, literature, music) was used as a theme for ownership and construction of self knowledge. We describe the results of the internship and its impact on the personal and professional education of future psychologists, highlighting the contributions triggered by the contact with Art...

El presente trabajo relata las experiencias vividas en una pasantía de Psicología escolar realizada en el Curso de Psicología de la Universidad Federal de Uberlândia (UFU). La pasantía, pautada en la Psicología histórico-cultural, estaba vinculada a la organización y al desarrollo de una disciplina titulada Psicología: Ciencia y Profesión, volcada a los alumnos que ingresaban en el referido curso. El contenido programático de esa disciplina va al encuentro de la necesidad de desarrollo de una visión crítica y socialmente comprometida con relación a la Psicología, lo que permite la constitución de un espacio de posibilidades de considerarse esa ciencia y profesión de un modo más amplio. Las actividades desarrolladas en los encuentros buscaban incitar reflexiones acerca de la Universidad, de la Psicología como ciencia y profesión y de la vida académica. Para la base esas discusiones, el Arte (cine, pintura, teatro, literatura, música) fue utilizado como recurso para la apropiación y la construcción del propio conocimiento. Describimos aquí la trayectoria de esa pasantía y sus repercusiones en la formación personal y profesional de los futuros psicólogos, dando destaque para las contribuciones desencadenadas desde el contacto con el Arte tanto para los practicantes como para la supervisora...

Pesquisa-intervenção em um CAPSad - Centro de Atenção Psicossocial Álcool e Drogas / Research-intervention in a CAPSad - Psychosocial Care Center for Alcohol and Drugs

O presente artigo irá traçar um processo de pesquisa-intervenção realizado ao longo de um ano em um Centro de Atenção Psicossocial - Álcool e Drogas (CAPSad). Esta pesquisa se deu a partir de práticas baseadas nos conceitos que envolvem a Análise Institucional, preconizando, assim, um trabalho de análise da complexa rede de relações de saber e poder presentes em tal serviço, bem como da dinâmica institucional que faz circular ali forças produtivas, ou instituintes, além de forças que tendem a perpetuarem-se ao que está instituído. Utilizamos o método de pesquisaintervenção por ser um método de pesquisa que indissocia o pesquisar do intervir, fazendo com que o percurso desta pesquisa se trace na processualidade e complexidade dos acontecimentos investigados. Nesse processo investigativo-interventivo, problematizou-se a clínica da Redução de Danos por se constituir como uma diretriz das políticas públicas nessa área(AU)

This article will outline a process of research-intervention carried out over a year in a Psychosocial Care Center for Alcohol and Drugs (CAPSad). This research took place on practices based on concepts that involve the Institutional Analysis, thus advocating a job analysis of the complex network of knowledge and power relations present in such service, as well as of the institutional dynamics which causes productive or instituting forces to circulate there, in addition to forces which tend to perpetuate themselves to what is established. We have used the method of research-intervention, for it is a method of research which dissociates the act of researching from the act of intervening, causing the route of this research to be traced in the processuality and complexity of the events investigated. In this investigative-intervening process, the clinic of the Damage Reduction became an issue, for it is a guideline for the public policies in this area(AU)

Pesquisa-intervenção em um CAPSad - Centro de Atenção Psicossocial Álcool e Drogas / Research-intervention in a CAPSad - Psychosocial Care Center for Alcohol and Drugs

O presente artigo irá traçar um processo de pesquisa-intervenção realizado ao longo de um ano em um Centro de Atenção Psicossocial - Álcool e Drogas (CAPSad). Esta pesquisa se deu a partir de práticas baseadas nos conceitos que envolvem a Análise Institucional, preconizando, assim, um trabalho de análise da complexa rede de relações de saber e poder presentes em tal serviço, bem como da dinâmica institucional que faz circular ali forças produtivas, ou instituintes, além de forças que tendem a perpetuarem-se ao que está instituído. Utilizamos o método de pesquisaintervenção por ser um método de pesquisa que indissocia o pesquisar do intervir, fazendo com que o percurso desta pesquisa se trace na processualidade e complexidade dos acontecimentos investigados. Nesse processo investigativo-interventivo, problematizou-se a clínica da Redução de Danos por se constituir como uma diretriz das políticas públicas nessa área.

This article will outline a process of research-intervention carried out over a year in a Psychosocial Care Center for Alcohol and Drugs (CAPSad). This research took place on practices based on concepts that involve the Institutional Analysis, thus advocating a job analysis of the complex network of knowledge and power relations present in such service, as well as of the institutional dynamics which causes productive or instituting forces to circulate there, in addition to forces which tend to perpetuate themselves to what is established. We have used the method of research-intervention, for it is a method of research which dissociates the act of researching from the act of intervening, causing the route of this research to be traced in the processuality and complexity of the events investigated. In this investigative-intervening process, the clinic of the Damage Reduction became an issue, for it is a guideline for the public policies in this area.