RESUMO
Two Gram-stain-negative, non-motile, non-pigmented cocci, designated IMCC11369(T) and IMCC11389, were isolated from surface seawater of the East Sea of Korea by high-throughput cultivation based on dilution to extinction. Strains IMCC11369(T) and IMCC11389 shared 99.9â% 16S rRNA gene sequence similarity and >86.3â% DNA-DNA relatedness, which suggested that they belong to the same genomic species. The isolates were most closely related to Lentisphaera araneosa HTCC2155(T) (99.0â% 16S rRNA gene sequence similarity). Phylogenetic analysis revealed that the isolates formed a robust cluster with L. araneosa HTCC2155(T). DNA-DNA relatedness values, however, showed that the isolates were distantly related to L. araneosa HTCC2155(T) (2.0-18.6â%), which suggested that they represent a separate genomic species in the genus Lentisphaera. The two isolates were phenotypically differentiated from their closest relative by several characteristics, including degradation of macromolecules and carbon source utilization. The DNA G+C content was 44.5-45.2 mol% and the predominant cellular fatty acids were C14â:â0, C16â:â1ω9c and C16â:â0. Strain IMCC11369(T) contained MK-7 as the respiratory quinone and phosphatidylethanolamine and an unknown lipid as the major polar lipids. On the basis of data obtained in this study, a novel species is proposed to accommodate the isolates, Lentisphaera marina sp. nov. The type strain is IMCC11369(T) (â=âKCTC 23780(T)â=âNBRC 108776(T)).
Assuntos
Bactérias/classificação , Filogenia , Água do Mar/microbiologia , Bactérias/genética , Bactérias/isolamento & purificação , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/análise , Dados de Sequência Molecular , Hibridização de Ácido Nucleico , Fosfatidiletanolaminas/análise , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/análiseRESUMO
The order "Parvularculales" represents the seventh order in the class Alphaproteobacteria. Parvularcula bermudensis, the type species of the order, was isolated from the Sargasso Sea using dilution-to-extinction culturing. We present here the complete genome sequence of Parvularcula bermudensis HTCC2503(T), which contains genes for carotenoid biosynthesis, dimethylsulfoniopropionate demethylase, and transduction-like gene transfer agents.
