RESUMO
The capacity to grow in a mineral medium with ethanol as a sole carbon source was studied in 147 Pseudomonas strains. All of the strains can grow in this medium. The biomass accumulation in the mineral medium with ethanol (1%) and maize extract (0.2%) is 2.5--5.0 times higher than without the latter. Strains which accumulate up to 3.5 g of dry biomass per litre (containing 62--66% of protein and 5.3% of nucleic acids) in the mineral medium with ethanol (1%) and maize extract (0.2%) have been selected. The quantitative content of essential amino acids satisfies the requirements while that of lysine, threonine, valine and leucine is twice as high.
Assuntos
Etanol/metabolismo , Pseudomonas/crescimento & desenvolvimento , Aminoácidos/análise , Proteínas de Bactérias/análise , Pseudomonas/análise , Pseudomonas/metabolismo , Pseudomonas aeruginosa/crescimento & desenvolvimento , Pseudomonas aeruginosa/metabolismo , Especificidade da EspécieRESUMO
Over 400 strains of Pseudomonads, when cultivated on starch media, were examined with respect to their capacity for extracellular production of amylases. Seven strains were selected that accumulated up to 6 g/l dry biomass and secreted amylase with an activity of up to 5.4 mg maltose/ml medium. The total and specific growth rate of the cultures was measured. Their physiological and biosynthetic activity in the course of development was evaluated. The economic coefficient was calculated. The dependence of the above parameters on the substrate concentration was demonstrated.