Drought tolerance memory transmission by citrus buds.

Plants face recurrent drought events, and previous stresses can influence their responses to subsequent stress episodes. Studies on drought stress memory are recent in citriculture, although they show promise as a tool for crop improvement. Here, we investigated whether stress memory mechanisms can be detected in citrus plants grafted with buds from plants subjected to recurrent water deficit. Three rootstock varieties, namely 'Rangpur Santa Cruz' lime, 'Sunki Maravilha' mandarin and 'Sunki Tropical' mandarin, in combination with 'Valencia' orange, were either maintained under full irrigation or subjected to one, two, or three water deficit cycles. Buds from 'Valencia' orange were grafted onto 'Swingle' citrumelo rootstocks and were evaluated. This combination displayed improved physiological and biochemical performance under water limitation, especially 'Valencia' buds grafted onto 'Sunki Maravilha', with better photosynthetic performance under water deficit. These findings indicate that genotype-dependent epigenetic memory is a key factor in restoring citrus plants' capacity to rely on previous stress experiences to restore better photosynthetic and physiological responses when undergoing new water deficit events. Therefore, epigenetic marks can be stored and transmitted to new citrus plants and are a promising alternative to enable increased water deficit tolerance when plants are then challenged by drought-prone environments.

Physiological and biochemical responses of mini watermelon irrigated with brackish water under two types of irrigation system / Respostas fisiológicas e bioquímicas de minimelancia irrigada com água salobra sob dois tipos de sistema de irrigação

The use of marginal quality water can be a viable alternative in regions with water scarcity when associated with an adequate irrigation management strategy. The aim of this study was to evaluate the physiological and biochemical responses of 'Sugar Baby' mini watermelon as a function of irrigation management and salinity of the nutrient solution (ECsol). The experiment was carried out in a greenhouse of the Federal University of Recôncavo of Bahia, in the municipality of Cruz das Almas - BA, in a completely randomized design, with four replications. The plants were grown under two types of irrigation management (conventional drip - CD and pulse - PD) and four saline levels of the fertigation nutrient solution (2.5 - control; 4.5; 5.5; 6.5 dS m-1). At 65 days after cultivation, the following variables were evaluated: chlorophyll a and b content, chlorophyll a fluorescence, and organic and inorganic solutes content. The treatments did not influence the levels of chlorophyll a and b. Salinity decreased the quantum yield of photochemical energy conversion due to the increased quantum yield of unregulated energy loss. Irrigation management and water salinity did not affect carbohydrate content in mini watermelons leaves. However, soluble proteins were higher in the CD than in PD and decreased with increasing salinity in both managements. Salinity increased free amino acids in CD but did not change the content of these solutes in PD. Free proline was only influenced by the management system and was higher in CD than in PD. Sodium, chloride, and sodium to potassium ratio increased with ECsol, but these increases were more pronounced in PD. Salinity increased potassium content in PD and reduced in CD. The CD led to lower absorption of toxic ions, reducing the effects of salinity on the mini watermelon.(AU)

A utilização de água da qualidade marginal pode ser uma alternativa viável em regiões com escassez hídrica, quando associada a estratégias de manejo de irrigação adequadas. O objetivo deste estudo foi avaliar as respostas fisiológicas e bioquímicas da minimelancia 'Sugar Baby' em função dos manejos de irrigação e da salinidade da solução nutritiva (CEsol). O experimento foi realizado em casa de vegetação da Universidade Federal do Recôncavo da Bahia, no município de Cruz das Almas, BA, Brasil, em delineamento experimental inteiramente casualizados com quatro repetições. As plantas foram cultivadas sob dois tipos de manejo de irrigação (gotejamento convencional - GC e por pulsos ­ GP) e quatro níveis salinos da solução nutritiva de fertirrigação (2,5 - controle; 4,5; 5,5; 6,5 dS m-1). Aos 65 dias após semeadura, foram avaliadas as variáveis: teores de clorofilas a e b, fluorescência da clorofila a e teores de solutos orgânicos e inorgânicos. Os tratamentos não influenciaram os teores de clorofila a e b. A salinidade diminuiu o rendimento quântico da conversão de energia fotoquímica com aumento na dissipação de energia não regulada, sendo que ambos são vias competitivas de energia entre si. O tipo de manejo e a salinidade não afetaram os teores de carboidratos nas folhas de minimelancia. Entretanto, as proteínas solúveis foram maiores no GC que no GP e diminuíram com o incremento da salinidade em ambos os manejos. A salinidade aumentou os aminoácidos livres no GC, mas não alterou o conteúdo destes solutos no GP. Os teores de prolina livre só foram influenciados pelo tipo de manejo, sendo maiores no GC que no GP. Os íons sódio e cloreto e a razão sódio/potássio aumentaram com a CEsol, sendo esses aumentos mais pronunciados no GP. A salinidade aumentou os teores de potássio no GP e diminuiu no GC. O GC levou a uma menor absorção de íons tóxicos, atenuando os efeitos da salinidade sobre a minimelancia.(AU)

Salt-tolerance induced by leaf spraying with H2O2 in sunflower is related to the ion homeostasis balance and reduction of oxidative damage.

Salinity is still one of the main factors that limit the growth and production of crops. However, currently, hydrogen peroxide (H2O2) priming has become a promising technique to alleviate the deleterious effects caused by salt. Therefore, this study aimed to test different leaf spraying strategies with H2O2 for acclimation of sunflower plants to salt stress, identifying the main physiological and biochemical changes involved in this process. The experiment was conducted in a completely randomized design, with four replications. Initially, four concentrations of H2O2 were tested (0.1; 1; 10 and 100 mM) associated with different applications: 1AP - one application (48 h before exposure to NaCl); 2AP - two applications (1AP + one application 7 days after exposure to NaCl) and 3AP - three applications (2AP + one application 14 days after exposure to NaCl), besides this two reference treatments were also added: control (absence of NaCl and absence of H2O2) and salt control (presence of 100 mM of NaCl and absence of H2O2). The experiment was conducted in hydroponic system containing Furlani's nutrient solution. Salt stress reduced the growth of sunflower plants, however, the H2O2 priming through leaf spraying was able to reduce the deleterious effects caused by salt, especially in the 1 mM H2O2 treatment with one application. H2O2 acts as a metabolic signal assisting in the maintenance of ionic and redox homeostasis, and consequently increasing the tolerance of plants to salt stress.

Photosynthesis and oxidative stress in the restinga plant species Eugenia uniflora L. exposed to simulated acid rain and iron ore dust deposition: potential use in environmental risk assessment.

The Brazilian sandy coastal plain named restinga is frequently subjected to particulate and gaseous emissions from iron ore factories. These gases may come into contact with atmospheric moisture and produce acid rain. The effects of the acid rain on vegetation, combined with iron excess in the soil, can lead to the disappearance of sensitive species and decrease restinga biodiversity. The effects of iron ore dust deposition and simulated acid rain on photosynthesis and on antioxidant enzymes were investigated in Eugenia uniflora, a representative shrub species of the restinga. This study aimed to determine the possible utility of this species in environmental risk assessment. After the application of iron ore dust as iron solid particulate matter (SPM(Fe)) and simulated acid rain (pH 3.1), the 18-month old plants displayed brown spots and necrosis, typical symptoms of iron toxicity and injuries caused by acid rain, respectively. The acidity of the rain intensified leaf iron accumulation, which reached phytotoxic levels, mainly in plants exposed to iron ore dust. These plants showed the lowest values for net photosynthesis, stomatal conductance, transpiration, chlorophyll a content and electron transport rate through photosystem II (PSII). Catalase and superoxide dismutase activities were decreased by simulated acid rain. Peroxidase activity and membrane injury increased following exposure to acid rain and simultaneous SPM(Fe) application. Eugenia uniflora exhibited impaired photosynthetic and antioxidative metabolism in response to combined iron and acid rain stresses. This species could become a valuable tool in environmental risk assessment in restinga areas near iron ore pelletizing factories. Non-invasive evaluations of visual injuries, photosynthesis and chlorophyll a fluorescence, as well as invasive biochemical analysis could be used as markers.

Técnica de transferência direta do molde da arcada superior do paciente para o articulador semi-ajustável / Direct molding transfer technique from patient's maxilary arch to semiadjustable articulator

Um dos erros mais comuns em reabilitação oral é a montagem incorreta dos modelos de gesso no articulador. Por isso, devemos também dar uma atenção especial nessa importante etapa, da mesma maneira que em qualquer outra fase do tratamento do paciente. Este trabalho apresenta uma técnica para transferência direta do molde da arcada superior do paciente para o articulador, conhecida como técnica Zanetti. Esse procedimento é realizado com o auxílio do conjunto Zanetti, o qual consegue transferir os registros da arcada superior, seja ela totalmente dentada, parcialmente ou totalmente desdentada, do paciente para o articulador, numa só etapa, sem a necessidade de confeccionarem-se bases de prova ou de registro, planos de orientação, utilização do garfo do arco facial e dos meios macios de transferência

A new method to simplify and increase the precision of maxillary cast mounting procedures in fully adjustable or semiadjustable articulators / Novo método simplificado para aprimorar a precisão na montagem de modelos em articulador totalmente ou semi ajustável

This study introduces a new impression procedure that permits mounting the maxillary stone cast in an articulator for any patient treatment and is applicable to any dental arch with any impression material. A transfer tray was designed that makes it possible to transfer the patient's maxillary impression to the articulator in a one-stage procedure without the need for making record bases and occlusion rims or the need to use a face-bow bite fork

Um novo método para simplificaçäo e maior precisäo de montagem de modelos de arcos superiores, em articuladores semi ou totalmente ajustáveis / A new method to simplify and increase the precision of maxillary cast mounting procedures on fully or semiadjustable articulators

Apresentaçäo de uma nova técnica de moldagem, obtençäo, transferência e montagem do modelo de gesso no articulador, em qualquer caso que exija a utilizaçäo de um articulador para o diagnóstico e planejamento do tratamento de pacientes. Os autores idealizaram uma moldeira de transferência, com a qual consegue-se transferir os registros da arcada superior do paciente para o articulador numa só etapa, sem a necessidade de confeccionarem-se bases de prova e planos de orientaçäo, utilizaçäo do garfo do arco facial e dos meios macios de transferência do modelo