The effect of local non-thermal plasma therapy on the cancer-immunity cycle in a melanoma mouse model.

Melanoma remains a deadly cancer despite significant advances in immune checkpoint blockade and targeted therapies. The incidence of melanoma is also growing worldwide, which highlights the need for novel treatment options and strategic combination of therapies. Here, we investigate non-thermal plasma (NTP), an ionized gas, as a promising, therapeutic option. In a melanoma mouse model, direct treatment of tumors with NTP results in reduced tumor burden and prolonged survival. Physical characterization of NTP treatment in situ reveals the deposited NTP energy and temperature associated with therapy response, and whole transcriptome analysis of the tumor identified several modulated pathways. NTP treatment also enhances the cancer-immunity cycle, as immune cells in both the tumor and tumor-draining lymph nodes appear more stimulated to perform their anti-cancer functions. Thus, our data suggest that local NTP therapy stimulates systemic, anti-cancer immunity. We discuss, in detail, how these fundamental insights will help direct the translation of NTP technology into the clinic and inform rational combination strategies to address the challenges in melanoma therapy.

Accuracy Assessment of Joint Angles Estimated from 2D and 3D Camera Measurements.

To automatically evaluate the ergonomics of workers, 3D skeletons are needed. Most ergonomic assessment methods, like REBA, are based on the different 3D joint angles. Thanks to the huge amount of training data, 2D skeleton detectors have become very accurate. In this work, we test three methods to calculate 3D skeletons from 2D detections: using the depth from a single RealSense range camera, triangulating the joints using multiple cameras, and combining the triangulation of multiple camera pairs. We tested the methods using recordings of a person doing different assembly tasks. We compared the resulting joint angles to the ground truth of a VICON marker-based tracking system. The resulting RMS angle error for the triangulation methods is between 12° and 16°, showing that they are accurate enough to calculate a useful ergonomic score from.

Camera Calibration Using Gray Code.

In order to determine camera parameters, a calibration procedure involving the camera recordings of a checkerboard is usually performed. In this paper, we propose an alternative approach that uses Gray-code patterns displayed on an LCD screen. Gray-code patterns allow us to decode 3D location information of points of the LCD screen at every pixel in the camera image. This is in contrast to checkerboard patterns where the number of corresponding locations is limited to the number of checkerboard corners. We show that, for the case of a UEye CMOS camera, the precision of focal-length estimation is 1.5 times more precise than when using a standard calibration with a checkerboard pattern.

Dynamic infrared thermography (DIRT) in Deep Inferior Epigastric Perforator (DIEP) flap breast reconstruction: standardization of the measurement set-up.

Breast reconstruction with an autologous free Deep Inferior Epigastric Perforator (DIEP) flap is one of the preferred options following mastectomy. A challenging step in this procedure is the selection of a suitable perforator that provides sufficient blood supply for the flap. The current golden standard for perforator mapping is computed tomography angiography (CTA). However, this is a relatively expensive imaging modality that requires intravenous contrast injection and exposes patients to ionizing radiation. More recently, dynamic infrared thermography (DIRT) has been proposed as an alternative imaging modality for perforator identification. DIRT appears to be an ideal alternative technique not only for the identification of the dominant perforators, but also for the mapping of the individual influence of each perforator on the flap perfusion. Multiple studies have been performed with the use of DIRT, unfortunately without standardisation of the measurement set-up. In this technical note we propose a standardised and reproducible measurement set-up for the use of DIRT during breast reconstructions with a free DIEP flap. This set-up can be used pre-, intra- and postoperatively. A standardised measurement set-up will improve the quality of measured data and ensure reproducibility.