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1.
Zookeys ; 1060: 125-153, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34629926

RESUMO

A list of amphibian and reptile species that occur in open and forested areas of the Atlantic Forest in the municipality of Cruz das Almas, in the Recôncavo Baiano, eastern Brazil is presented. Field sampling occurred between January 2015 to March 2019, totalling 117 samples distributed in three areas: Parque Florestal Mata de Cazuzinha, Mata da Cascalheira, and Riacho do Machado. A total of 1,848 individuals of 69 species (31 anurans, 14 lizards, 19 snakes, two amphisbaenians, and three testudines) was recorded. Additionally, one individual of Ophiodesstriatus was found in Mata da Cascalheira after the end of sampling, totalling 15 lizard species and 70 herpetofaunal species. The prevalence of open-area species and the presence of Phyllopezuslutzae, Diploglossuslessonae, and Dryadosauranordestina in interior forest patches are discussed. Additionally, a new record of the invasive terrapin Trachemysdorbigni in the State of Bahia is reported.

2.
Nanomedicine ; 14(6): 1753-1763, 2018 08.
Artigo em Inglês | MEDLINE | ID: mdl-29778889

RESUMO

We present a methodology for production and application of electrospun hybrid materials containing commercial polyester (poly (butylene adipate-co-terephthalate; PBAT), and a conductive polymer (polypyrrole; PPy) as scaffold for neuronal growth and differentiation. The physical-chemical properties of the scaffolds and optimization of the electrospinning parameters are presented. The electrospun scaffolds are biocompatible and allow proper adhesion and spread of mesenchymal stem cells (MSCs). Fibers produced with PBAT with or without PPy were used as scaffold for Neuro2a mouse neuroblastoma cells adhesion and differentiation. Neuro2a adhered to PBAT and PBAT/PPy2% scaffolds without laminin coating. However, Neuro2a failed to differentiate in PBAT when stimulated by treatment with retinoic acid (RA), but differentiated in PBAT/PPy2% fibers. We hypothesize that PBAT hydrophobicity inhibited proper spreading and further differentiation, and inhibition was overcome by coating the PBAT fibers with laminin. We conclude that fibers produced with the combination of PBAT and PPy can support neuronal differentiation.


Assuntos
Células-Tronco Mesenquimais/patologia , Nanofibras/química , Neuritos/patologia , Neuroblastoma/patologia , Poliésteres/administração & dosagem , Polímeros/administração & dosagem , Pirróis/administração & dosagem , Animais , Adesão Celular/efeitos dos fármacos , Diferenciação Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Células-Tronco Mesenquimais/efeitos dos fármacos , Camundongos , Camundongos Endogâmicos C57BL , Neuritos/efeitos dos fármacos , Neuroblastoma/tratamento farmacológico , Poliésteres/química , Polímeros/química , Pirróis/química , Alicerces Teciduais , Células Tumorais Cultivadas
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