Ration particle size has different effects on digestive but not production parameters in higher-yielding (Holstein) compared to lower-yielding (Girolando) cows.

The aim of the study was to evaluate the effect of total mixed ration particle size (length) and breed of cow on intake dynamics, animal performance and CH4 emissions, comparing high yielding Holstein and low yielding Girolando cows. The experimental design was 2 × 2 Latin Square arranged as a crossover factorial scheme with two diets (short particle size, SPS and long particle size, LPS) and the two breed compositions. The design comprised two periods of 26 d each, where all data collection was performed at cow level. No influence of the particle size occurred for the passage rate, neutral detergent fiber digestibility, performance and milk composition, methane emissions or ruminal fermentation parameters. Girolando cows had greater dry matter intake (DMI) when fed SPS, while Holsteins had the same (P < 0.05). Girolando cows had lower dry matter digestibility when fed LPS compared to SPS, while Holsteins had the opposite effect (P < 0.05). Also, the digestibility of crude protein and non-fibrous carbohydrates decreased in Girolando cows fed LPS, but not in Holsteins (P < 0.05). Girolando cows reduced DMI by 10.6% when fed LPS diet (P < 0.05). Girolando had an increased eating rate (+24 g of DM/min; P < 0.05) compared to Holstein cows, but Holstein cows had a lower CH4 intensity (by 29.7%: P < 0.05). Girolando cows increased the dry matter intake when fed a diet with short particle size, while the same did not happen in Holsteins. Dry matter digestibility increased in Holsteins when fed long particle size, while the opposite was observed in Girolando cows. Nutrient digestibility was reduced in Girolando cows when fed short particle size. Particle size did not influence eating time, eating rate, feed trough visits, visits with intake, milk yield and composition regardless of the breed. Reducing particle size increased CH4 intensity in both breeds.

Replacement of Soybean Meal With Soybean Cake Reduces Methane Emissions in Dairy Cows and an Assessment of a Face-Mask Technique for Methane Measurement.

The objective of this study was to (a) evaluate the effect of replacing soybean meal (SBM) with soybean cake (SBC) on feeding behavior, rumen fermentation, milk production, nutrient digestibility and CH4 emissions and (b) investigate whether a face-mask technique could be used to predict daily methane (CH4) emissions in dairy cattle. The experiment was conducted as a completely randomized design, with 32 crossbred Holstein × Gyr cows (days in milk (DIM): 112 ± 25.1) randomly assigned to the following treatments (n = 8/group) for 75 days: (1) 0% SBC, (2) 6% SBC, (3) 14% SBC, and (4) 23% SBC, in place of SBM on a dry matter (DM) basis. Across the final 4 weeks of the study, CH4 production was estimated using the proposed face-mask technique subsequent to a respiration chamber measurement for an evaluation of treatment efficacy and face-mask accuracy. There was no effect of SBM replacement by SBC on intake, feeding or drinking behavior (P > 0.21). Total VFA concentration, the individual proportions of VFA and blood metabolites were not altered (P > 0.17) by SBC, however there was a tendency for decreased (P = 0.08) lactate and plasma urea nitrogen (P = 0.07) concentration associated with SBC addition. Fat-corrected milk yield (FCM4%) and composition was not affected (P > 0.27) by SBC; however, there was a tendency for decreased total milk solids (P = 0.07) and milk fat (P = 0.08) associated with 23% SBC treatment. There was no treatment × technique interaction (P > 0.05) effect on gas measurements. A maximum reduction (P = 0.01) in CH4 yield (g/kg DM) and intensity (g/kg milk) of 11 and 20%, respectively, was observed for the 14% SBC inclusion. Compared to the week of mask measurements, chambers decreased (P = 0.01) intake (kg/d, %BW) and increased (P = 0.05) FCM4%. The face-mask method over estimated O2 consumption by 5%. The face-mask method accurately predicted daily CH4 emissions when compared to the chamber at the same time-point. However, there was a linear bias of CH4 outputs so further evaluation of the calculation of total CH4 from a spot measurement is required.

Tithonia diversifolia as a Supplementary Feed for Dairy Cows.

The objective of this study was to examine the effects of Tithonia diversifolia as a supplementary forage on dairy cow performance and methane production. Nine lactating Holstein × Zebu dairy cows (519 ± 53.3 kg of body weight and 66 ± 13.3 d in milk) were paired by milk yield (21.3 ± 2.34 kg/d) and body weight and randomly assigned to three dietary treatments in a Latin square design with 21-d experimental periods (14 d for diet adaptation and 7 d for measurements and sample collection). The dietary treatments included the control diet consisting of fresh sugar cane plus concentrate (44:56, % of diet DM), and two treatment diets containing different levels of fresh T. diversifolia (6.5 and 15.4%, DM basis) which partially replaced both sugarcane and concentrates. Methane production was measured using the sulphur hexafluoride (SF6) technique from d 16 to d 21 of each experimental period. Analysis of the gas samples was performed by gas chromatography. The inclusion of T. diversifolia at 15.4% DM had no effects on DM intake, milk production, nitrogen balance or methane production. There was no effect on the concentrations of total saturated fatty acids (SFA), monounsaturated fatty acids (MUFA), and polyunsaturated fatty acids (PUFA) in milk fat (P ≥ 0.28), though individual milk fatty acids were affected. Serum concentrations of glucose, urea nitrogen (BUN), triglycerides, ß-hydroxybutyrate (BHBA), and cholesterol were unaffected by the dietary treatments (P ≥ 0.13). There was a time (2 and 6 h post-feeding) and dietary treatment effect (P < 0.01) on the acetate to propionate ratio in the rumen. A denaturing gradient gel electrophoresis analysis of the archaeal community showed distinct clustering of the archaea populations for control and treatment diets. Taken together, our results indicate the potential of T. diversifolia as a supplementary forage for dairy cattle in the tropics.

Productivity and nutritional quality of Flechinha grass ( Echinolaena inflexa ), native grass of Brazilian Cerrado / Produção e qualidade nutricional do capim-flechinha (Echinolaena inflexa), gramínea nativa do Cerrado brasileiro

ABSTRACT: Due to scarce nutritional data, this study assessed the productivity and nutritional value of Echinolaena inflexa (EI) grass, native to the Cerrado biome. It was compared to B. brizantha (BB), one of the most cultivated grasses in Brazil, during a whole year (rainy; RS and dry season; DS). Sampling was held in accordance with pasture management (entry / exit height; 50 / 5cm and 80 / 25cm for EI and BB, respectively). Dry matter production (DMP), crude protein (CP), neutral and acid detergent fiber (NDF; ADF), hemicellulose (HCEL), PB insoluble neutral and acid detergent (PIDN; PIDA), total and non-fibrous carbohydrates (TC; NFC), ether extract (EE), and mineral matter (MM), and in vitro fermentation kinetics and DM degradability (DMD) were evaluated. A completely randomized design (season as a fixed term) and average treatment compared by Tukey post test were applied. EI produced 38.5% of the DMP of BB. A higher CP (75.3; 73.5 in the RS and DS), PIDA (12.5; 8.7), PIDN (47.1; 40.1), NDF (714.4; 749.5) and ADF (396.0; 419.0) were obtained by EI in relation to BB (CP (60.3; 33.5), PIDA (6.0; 3.5), PIDN (21.4; 10.8), NDF (673.0; 675.1) and ADF (335.5; 351.4) during the RS and DS, respectively (g kg-1 DM). In vitro data were directly associated with chemical composition, resulting in lower DMD of EI compared to BB. EI showed productive similarity (DMP) during RS and DS (939.3; 809.8kg DM respectively). Although EI showed greater nutritional stability (CP) between seasons, 17% of CP was linked to ADF and therefore, not available for rumen microorganisms.

RESUMO: Existem escassas informações a respeito da qualidade nutricional e potencial produtivo de Echinolaena Inflexa, planta nativa do Cerrado Brasileiro e que possui potencial valor forrageiro. Dessa forma, este trabalho objetivou avaliar o seu valor nutritivo e produtividade, comparando-a com a B. brizantha (BB), uma das mais cultivadas gramíneas no Brasil, durante um no agrícola (Estação das Chuvas; CH e seca; SE). A coleta do material experimental foi realizada de acordo com as recomendações de manejo (altura de entrada / altura de saída 50/5cm e 80/25cm para EI e BB, respectivamente). Foram avaliadas a produção de matéria seca (PMS), proteína bruta (PB), fibra detergente neutro e ácido (FDN e FDA), hemicelulose (HCEL), PB insolúvel em detergente neutro e ácido (PIDN e PIDA), carboidratos totais e não fibrosos (CT e CNF), extrato etéreo (EE) e matéria mineral (MM), e a cinética de fermentação ruminal e degradabilidade in vitro da MS (DMS). Utilizou-se o delineamento inteiramente casualizado e as médias de tratamento comparadas pelo post test de Tukey. A EI apresentou 38,5% da PMS da BB. Foram obtidos maiores teores de PB (75,3 e 73,5), PIDA (12,5 e 8,7), PIDN (47,1 e 40,1), FDN (714,4 e 749,5) e FDA (396,0 e 419,0) para a EI em relação a BB (PB; 60,3 e 33,5), PIDA (6,0 e 3.5), PIDN (21,4 e 10,8), FDN (673,0 e 675,1) e FDA (335,5 e 351,4) na estação chuvosa e seca, respectivamente (g kg-1 MS). Os dados in vitro da EI foram diretamente associados à composição química, resultando em menor DMS comparativamente BB. A EI apresentou estabilidade produtiva (PMS) durante a estação chuvosa e seca (939,3 e 809,8kg MS), respectivamente. Apesar da similaridade nutricional (PB) nas estações, cerca de 17% da PB está ligada a FDA e, portanto, não disponível para os microrganismos ruminais.