Exploring the use of visual predictions in social scenarios while under anticipatory threat.

One of the less recognized effects of anxiety lies in perception alterations caused by how one weighs both sensory evidence and contextual cues. Here, we investigated how anxiety affects our ability to use social cues to anticipate the others' actions. We adapted a paradigm to assess expectations in social scenarios, whereby participants were asked to identify the presence of agents therein, while supported by contextual cues from another agent. Participants (N = 66) underwent this task under safe and threat-of-shock conditions. We extracted both criterion and sensitivity measures as well as gaze data. Our analysis showed that whilst the type of action had the expected effect, threat-of-shock had no effect over criterion and sensitivity. Although showing similar dwell times, gaze exploration of the contextual cue was associated with shorter fixation durations whilst participants were under threat. Our findings suggest that anxiety does not appear to influence the use of expectations in social scenarios.

Radiological presentation of active pulmonary tuberculosis in kidney transplant recipients: a retrospective study of four cases and a review of the literature / Apresentação radiológica da tuberculose pulmonar ativa em transplantados renais: um estudo retrospectivo de quatro casos e revisão da literatura

Abstract Although kidney transplantation is the best therapeutic option for patients with chronic kidney disease, the immunosuppression required greatly increases susceptibility to infections that are responsible for high post-transplant mortality. Pulmonary tuberculosis (TB) represents a major cause of such infections, and its early diagnosis is therefore quite important. In view of that, we researched the manifestations of active pulmonary TB in kidney transplant recipients, through chest X-ray and computed tomography (CT), as well as determining the number of cases of active pulmonary TB occurring over a 3.5-year period at our institution. We identified four cases of active pulmonary TB in kidney transplant recipients. The CT scans provided information complementary to the chest X-ray findings in all four of those cases. We compared our CT findings with those reported in the literature. We analyzed our experience in conjunction with an extensive review of the literature that was nevertheless limited because few studies have been carried out in lowand middle-income countries, where the incidence of TB is higher.

Resumo Apesar de o transplante renal ser a melhor opção terapêutica para pacientes com doença renal crônica, a imunodepressão decorrente desse tratamento eleva muito a suscetibilidade desses pacientes a infecções, responsáveis por altas taxas de mortalidade pós-operatórias. A tuberculose (TB) pulmonar é uma significativa causa dessas infecções, sendo muito importante o seu diagnóstico precoce. Assim, nós pesquisamos as manifestações da TB pulmonar ativa nessa população de transplantados renais por meio de radiografias simples e tomografia computadorizada (TC) do tórax, também para estabelecer o número de casos de TB pulmonar ativa em nossa instituição após levantamento de 3,5 anos. Encontramos quatro casos de TB pulmonar ativa em pacientes transplantados renais. A TC forneceu informações adicionais em relação às radiografias de tórax em 100% dos casos analisados. Comparamos os nossos achados de TC com os relatados na literatura. Somamos a experiência obtida com extensa revisão da literatura, ainda limitada nessa questão, com poucos estudos realizados em países em desenvolvimento onde a incidência de TB é maior.

ER-trafficking triggers NRF1 ubiquitination to promote its proteolytic activation.

The transcription factor NRF1 resides in the endoplasmic reticulum (ER) and is constantly transported to the cytosol for proteasomal degradation. However, when the proteasome is defective, NRF1 escapes degradation and undergoes proteolytic cleavage by the protease DDI2, generating a transcriptionally active form that restores proteostasis, including proteasome function. The mechanisms that regulate NRF1 proteolytic activation and transcriptional potential remain poorly understood. This study demonstrates that the ER is a crucial regulator of NRF1 function by orchestrating its ubiquitination through the E3 ubiquitin ligase HRD1. We show that HRD1-mediated NRF1 ubiquitination is necessary for DDI2-mediated processing in cells. Furthermore, we found that deficiency in both RAD23A and RAD23B impaired DDI2-mediated NRF1 processing, indicating that these genes are essential components of the DDI2 proteolytic machinery. Our findings highlight the intricate mechanism by which the ER activates NRF1 to coordinate the transcriptional activity of an adaptation response in cells.

Impact of impaired pulmonary function on clinical outcomes in survivors of severe COVID-19 without pre-existing respiratory disease.

OBJECTIVE: To investigate the impact of impaired pulmonary function on patient-centered outcomes after hospital discharge due to severe COVID-19 in patients without preexisting respiratory disease. METHODS: This is an ongoing prospective cohort study evaluating patients (> 18 years of age) 2-6 months after hospital discharge due to severe COVID-19. Respiratory symptoms, health-related quality of life, lung function, and the six-minute walk test were assessed. A restrictive ventilatory defect was defined as TLC below the lower limit of normal, as assessed by plethysmography. Chest CT scans performed during hospitalization were scored for the presence and extent of parenchymal abnormalities. RESULTS: At a mean follow-up of 17.2 ± 5.9 weeks after the diagnosis of COVID-19, 120 patients were assessed. Of those, 23 (19.2%) reported preexisting chronic respiratory diseases and presented with worse lung function and exertional dyspnea at the follow-up visit in comparison with their counterparts. When we excluded the 23 patients with preexisting respiratory disease plus another 2 patients without lung volume measurements, a restrictive ventilatory defect was observed in 42/95 patients (44%). This subgroup of patients (52.4% of whom were male; mean age, 53.9 ± 11.3 years) showed reduced resting gas exchange efficiency (DLCO), increased daily-life dyspnea, increased exertional dyspnea and oxygen desaturation, and reduced health-related quality of life in comparison with those without reduced TLC (50.9% of whom were male; mean age, 58.4 ± 11.3 years). Intensive care need and higher chest CT scores were associated with a subsequent restrictive ventilatory defect. CONCLUSIONS: The presence of a restrictive ventilatory defect approximately 4 months after severe COVID-19 in patients without prior respiratory comorbidities implies worse clinical outcomes.

Impact of impaired pulmonary function on clinical outcomes in survivors of severe COVID-19 without pre-existing respiratory disease / Impacto do comprometimento da função pulmonar nos desfechos clínicos em sobreviventes de COVID-19 grave sem doenças respiratórias preexistentes

ABSTRACT Objective: To investigate the impact of impaired pulmonary function on patient-centered outcomes after hospital discharge due to severe COVID-19 in patients without preexisting respiratory disease. Methods: This is an ongoing prospective cohort study evaluating patients (> 18 years of age) 2-6 months after hospital discharge due to severe COVID-19. Respiratory symptoms, health-related quality of life, lung function, and the six-minute walk test were assessed. A restrictive ventilatory defect was defined as TLC below the lower limit of normal, as assessed by plethysmography. Chest CT scans performed during hospitalization were scored for the presence and extent of parenchymal abnormalities. Results: At a mean follow-up of 17.2 ± 5.9 weeks after the diagnosis of COVID-19, 120 patients were assessed. Of those, 23 (19.2%) reported preexisting chronic respiratory diseases and presented with worse lung function and exertional dyspnea at the follow-up visit in comparison with their counterparts. When we excluded the 23 patients with preexisting respiratory disease plus another 2 patients without lung volume measurements, a restrictive ventilatory defect was observed in 42/95 patients (44%). This subgroup of patients (52.4% of whom were male; mean age, 53.9 ± 11.3 years) showed reduced resting gas exchange efficiency (DLCO), increased daily-life dyspnea, increased exertional dyspnea and oxygen desaturation, and reduced health-related quality of life in comparison with those without reduced TLC (50.9% of whom were male; mean age, 58.4 ± 11.3 years). Intensive care need and higher chest CT scores were associated with a subsequent restrictive ventilatory defect. Conclusions: The presence of a restrictive ventilatory defect approximately 4 months after severe COVID-19 in patients without prior respiratory comorbidities implies worse clinical outcomes.

RESUMO Objetivo: Investigar o impacto do comprometimento da função pulmonar nos desfechos centrados no paciente após a alta hospitalar em pacientes sem doenças respiratórias preexistentes que foram hospitalizados em virtude de COVID-19 grave. Métodos: Trata-se de um estudo prospectivo de coorte em andamento, no qual pacientes com COVID-19 grave (com idade > 18 anos) são avaliados 2-6 meses depois da alta hospitalar. Avaliamos os sintomas respiratórios, a qualidade de vida relacionada à saúde, a função pulmonar e a distância percorrida no teste de caminhada de seis minutos. A definição de distúrbio ventilatório restritivo foi CPT abaixo do limite inferior da normalidade na pletismografia. As imagens de TC de tórax realizadas durante a hospitalização foram avaliadas quanto à presença e extensão de alterações parenquimatosas. Resultados: Em média 17,2 ± 5,9 semanas depois do diagnóstico de COVID-19, foram avaliados 120 pacientes. Destes, 23 (19,2%) relataram doenças respiratórias crônicas preexistentes e apresentaram pior função pulmonar e maior dispneia aos esforços na consulta de acompanhamento quando comparados aos outros participantes. Quando excluímos os 23 pacientes com doenças respiratórias preexistentes e mais 2 pacientes (sem medidas de volumes pulmonares), observamos distúrbio ventilatório restritivo em 42/95 pacientes (44%). Esse subgrupo de pacientes (52,4% dos quais eram do sexo masculino, com média de idade de 53,9 ± 11,3 anos) apresentou menor eficiência das trocas gasosas (DLCO), maior dispneia na vida diária e dessaturação de oxigênio ao exercício e redução da qualidade de vida relacionada à saúde em comparação com aqueles sem redução da CPT (50,9% dos quais eram do sexo masculino, com média de idade de 58,4 ± 11,3 anos). A necessidade de terapia intensiva e pontuações mais altas no escore de alterações parenquimatosas na TC de tórax apresentaram relação com distúrbio ventilatório restritivo subsequente. Conclusões: A presença de distúrbio ventilatório restritivo aproximadamente 4 meses depois da COVID-19 grave em pacientes sem comorbidades respiratórias prévias implica piores desfechos clínicos.

A scoping study of pulmonary paracoccidioidomycosis: severity classification based on radiographic and tomographic evaluation.

The lungs have great importance in patients with paracoccidioidomycosis since they are the portal of entry for the infecting fungi, the site of quiescent foci, and one of the most frequently affected organs. Although they have been the subject of many studies with different approaches, the severity classification of the pulmonary involvement, using imaging procedures, has not been carried out yet. This study aimed to classify the active and the residual pulmonary damage using radiographic and tomographic evaluations, according to the area involved and types of lesions.

The protease DDI2 regulates NRF1 activation in response to cadmium toxicity.

DNA-damage inducible 1 homolog 2 (DDI2) is a protease that activates the transcription factor NRF1. Cellular models have shown that this pathway contributes to cell-stress adaptation, for example, on proteasome inhibition. However, DDI2 physiological function is unknown. Ddi2 Knock-out (KO) mice were embryonic lethal. Therefore, we generated liver-specific Ddi2-KO animals and used comprehensive genetic analysis to identify the molecular pathways regulated by DDI2. Here, we demonstrate that DDI2 contributes to metallothionein (MT) expression in mouse and human hepatocytes at basal and upon cadmium (Cd) exposure. This transcriptional program is dependent on DDI2-mediated NRF1 proteolytic maturation. In contrast, NRF1 homolog NRF2 does not contribute to MT production. Mechanistically, we observed that Cd exposure inhibits proteasome activity, resulting in DDI2-mediated NRF1 proteolytic maturation. In line with these findings, DDI2 deficiency sensitizes cells to Cd toxicity. This study identifies a function for DDI2 that links proteasome homeostasis to heavy metal mediated toxicity.

Exercise training-induced changes in immunometabolic markers in youth badminton athletes.

The aim of this study was to investigate the metabolic and inflammatory fluctuations in two seasonal phases of badminton training, and the ability of youth badminton athletes to respond to an inflammatory challenge given by acute exercise on these markers. Thirteen youth badminton athletes who participated in national and international competitions were recruited. Metabolic and cytokine profile were measured at rest and in response to a maximal exercise intermittent test, in the pre- and final phases of a badminton annual season. At rest, glucose (-7.58 mg/dL; p = 0.045) and HDL-cholesterol (HDL-c) (-26.87 mg/dL; p < 0.0001) decreased at final-season. The variation of HDL-c in response to a maximal exercise test increased at final-season in comparison to pre-season (+ 10.20 mg/dL p = 0.042). Similarly, delta changes of IL-10 (+ 3.41 pg/ml; p = 0.047) and IL-1Ra (+ 141.3 pg/ml; p = 0.031) were greater at final-season. In addition, a significantly greater variation of the anti-inflammatory IL-10/IL-17 ratio was observed at final-season (+ 0.37; p = 0.010). In conclusion, our results showed a major responsivity of IL-10 and IL-1Ra to a maximal exercise even at the end of an entire season. The major responsivity of these cytokines at this time point suggests a more effective acute inflammatory response in youth badminton athletes. Therefore, the results of this study may be applied by coaches, trainers and sport nutritionist for proper training management.

Agreement between histopathological and intraoperative classifications for pediatric appendicitis and its relationship with the post-operative clinical outcome.

Introduction: Some studies have shown poor agreement between intraoperative and histopathological classifications for appendicitis, despite their routine use in clinical practice. Objective: To investigate the agreement between histopathological and intraoperative classifications for pediatric appendicitis and evaluate the predictive potential of these classifications for the post-operative outcome. Methods: A retrospective, longitudinal, observational single-center study, carried out with 485 patients up to15 years of age, with a confirmed diagnosis of acute appendicitis by histopathological evaluation. The histopathological results classified the appendices as uncomplicated appendicitis when there was confirmation of the diagnosis of appendicitis without necrosis or perforation and complicated appendicitis when there was extensive necrotic tissue in the outer layer of the appendix or signs of perforation. The intraoperative findings were classified as uncomplicated appendicitis when the appendix presented with hyperemia and edema or fibrinous exudate and complicated appendicitis when the appendix showed necrosis, abscess, or perforation. The kappa index determined the agreement and the prediction relationships using a generalized linear model. Results: 43.9% of cases were classified as complicated appendicitis by histopathological evaluation and 49.7% by intraoperative classification. The agreement analysis between the histopathological and intraoperative classification showed a moderate agreement, with a Kappa index of 0.419 (0.337-0.501). There was an association (P < 0.05) between the intraoperative classification and the post-operative clinical outcomes (time to start feeding, fever, intraabdominal collection, length of stay, the need for antibiotic therapy changing, and need for ICU). There was no association between histopathological classification and post-operative outcomes. Conclusion: The agreement between the two classifications was moderate, and the intraoperative classification was able to predict the post-operative clinical outcomes.

Phase angle is moderately correlated with lower-body power and fitness capacity in junior Badminton players / Ángulo de fase se correlaciona moderadamente con la potencia de los miembros inferiores y la condiciónfísica en jugadores de Bádminton junior / Ângulo de fase está moderadamente correlacionado com potência dos membros inferiores e aptidão física em jogadores juniores de Badminton

Objective: Phase angle (PhA) is derived from bioimpedance analysis (BIA) and is widely used as an indicator of cellular health, cell membrane integrity,and cell function. Lower-body power and fitness capacity are of paramount importance in success in several sports, including badminton. This studyaimed to evaluate the relationship between PhA and lower-body power and fitness capacity in 22 junior badminton players (14 males, 8 females, 17.7 ±1.4 years old). Methods: Bioelectrical impedance was used to assess body fat (BF), muscle mass (MM) and PhA. Countermovement jump test and Yo-yo intermittent testlevel 2 were used to evaluate lower-body power and fitness capacity, respectively. Pearson’s correlation was used to assess the relationship between PhAand lower-body power and fitness capacity, with multiple regressions considering the effect of BF, MM, and age.Results: PhA exhibited a positive relationship with lower-body power ( = 0.48; p < 0.02) and fitness capacity ( = 0.37; p < 0.04). However, theseβ = 0.48; p < 0.02) and fitness capacity (β = 0.37; p < 0.04). However, theseβ = 0.48; p < 0.02) and fitness capacity (β = 0.37; p < 0.04). However, theserelationships lost significance after adjustment for the co-variables MM, BF, and age (p > 0.24). Conclusion: PhA is associated with lower-body power and fitness capacity in junior badminton players. However, these relationships are influenced byMM, BF, and age co-variables. (AU)

Objetivo: El ángulo de fase (AF) se deriva del análisis de bioimpedancia (BIA) y es usado ampliamente como indicador de la salud celular, la integridad dela membrana celular y la función celular. La potencia de los miembros inferiores y la condición física son de gran importancia para el éxito en variosdeportes, incluido el bádminton. El objetivo de este estudio fue evaluar la relación entre el AF y la potencia de los miembros inferiores y la condiciónfísica en 22 jugadores de bádminton junior (14 hombres, 8 mujeres, 17.7 ± 1.4 años). Método: Se utilizó la bioimpedancia eléctrica para evaluar la grasa corporal (GC), la masa muscular (MM) y el AF. La prueba de salto en contramovimientoy la prueba YoYo de recuperación intermitente nivel 2 fueran utilizados para evaluar la potencia de los miembros inferiores y la condición física,respectivamente. Se calculó la Correlación de Pearson para evaluar a relación entre el AF y la potencia de los miembros inferiores y condición física, conregresiones múltiples considerando el efecto de GC, MM y edad. Resultados: AF mostró una relación positiva con la potencia de los miembros inferiores (β = 0.48; p < 0.02) and fitness capacity (β = 0.37; p < 0.04). However, these = 0.48; p < 0.02) y la condición física (β = 0.48; p < 0.02) and fitness capacity (β = 0.37; p < 0.04). However, these = 0.37; p <0.04). Sinembargo, estas relaciones perdieron significación después de ajustadas con las covariables MM, GC y edad (p > 0.24). Conclusión: El ángulo de fase se asocia con la potencia de los miembros inferiores y la condición física de jugadores de bádminton juveniles. Sin embargo,estas relaciones son influenciadas por las covariables masa muscular, grasa corporal y edad.(AU)

Objetivo: O ângulo de fase (AF) é derivado da análise de bioimpedância (BIA) e é amplamente usado como um indicador de saúde celular, integridade damembrana celular e função celular. A força dos membros inferiores e aptidão física são de grande importância para o sucesso em vários esportes,incluindo o badminton. O objetivo deste estudo foi avaliar a relação entre a AF e a potência dos membros inferiores e a aptidão física em 22 jogadoresjuniores de badminton (14 homens, 8 mulheres, 17,7 ± 1,4 anos). Método: A bioimpedância elétrica foi utilizada para avaliar gordura corporal (GC), massa muscular (MM) e AF. O teste de salto com contramovimento e oteste intermitente Yo-yo nível 2 foram usados para avaliar a potência dos membros inferiores e a aptidão física, respectivamente. Correlação de Pearsonfoi usada para avaliar a relação entre AF e potência dos membros inferiores e aptidão física, com regressões múltiplas considerando o efeito do GC, MM eidade. Resultados: AF exibiu uma relação positiva com a potência dos membros inferiores (β = 0.48; p < 0.02) and fitness capacity (β = 0.37; p < 0.04). However, these = 0.48; p <0.02) e aptidão física (β = 0.48; p < 0.02) and fitness capacity (β = 0.37; p < 0.04). However, these = 0.37; p < 0.04). No entanto,essas relações perderam a significância após o ajuste para as covariáveis MM, GC e idade (p > 0.24). Conclusões: AF está associado à dos membros inferiores e à capacidade física de jogadores juniores de badminton. No entanto, essas relações sãoinfluenciadas pelas covariáveis MM, GC e idade.(AU)

Detraining and retraining in badminton athletes following 1-year COVID-19 pandemic on psychological and physiological response.

Purpose: Badminton is a racket sport, with fast and explosive movements and mental skills employed to anticipate the opponent's movements. The COVID-19 pandemic, led to social restriction in Brazil and sport event cancellations, subsequently, sports training was banned. Thus, the objective of this study was to compare the impact of long-period detraining due to COVID-19 social restriction (8 months and 1-year) on cardiorespiratory fitness, body composition, nutritional behavior, and profile of mood states in badminton athletes and to verify if the athletes who returned to their regular training 4 months earlier than athletes who stopped their daily training routine during 1-year would improve these variables. Methods: Twenty-three young badminton athletes were analyzed: retrained group (14 athletes who stopped their daily training routine for 8 months due to the COVID-19 pandemic plus 4 months of retraining), and detrained group (9 athletes who stopped their daily training routine during 1 year of the COVID-19 pandemic but performed home-based training). We evaluated body composition, cardiorespiratory fitness, nutritional behavior, and mood states profiles. Results: Retrained athletes showed lower body fat (- 24.1% vs. + 20.8%, p < 0.001) and higher fat-free mass (+ 6.0% vs. - 0.2%, p = 0.007) after 1 year compared with the detrained group. For cardiorespiratory fitness [retrained: baseline = 55.5 ± 5.3 (47.1, 63.9) and after 1 year = 58.1 ± 2.4 (54.2, 61.9), ES = 0.65 vs. detrained: baseline = 53.4 ± 6.7 (47.2, 59.5) and after 1 year = 53.1 ± 5.6 (48.0, 58.3), ES = - 0.03] and nutritional behavior, including sauces and spices [retrained: baseline = 8.9 ± 7.0 (4.5, 13.4), and after 1 year = 3.4 ± 2.9 (1.8, 5.5), ES = - 1.11 vs. detrained: baseline = 6.8 ± 6.7 (1.6, 11.9) and after 1 year = 6.3 ± 5.5 (2.1, 10.6), ES = - 0.08], the ESs were medium and large, respectively, for Retrained but trivial for detrained group. For depression, ES was trivial in the retrained [baseline = 2.7 ± 3.3 (0.7, 4.7) and after 1 year = 2.6 ± 2.9 (0.8, 4.4), ES = 0.03] and moderate for detrained [baseline = 1.0 ± 1.5 (- 0.1, 2.1) and after 1 year = 1.8 ± 2.7 (- 0.3, 3.8), ES = 0.50]. Conclusions: Young badminton athletes who returned to their regular daily training 4 months earlier than athletes who stopped their daily training routine during 1-year due to COVID-19 social restriction decreased fat mass and increased fat-free mass. There were no significant differences between groups for cardiorespiratory fitness, nutritional behavior, and profile of mood state response.

The aspartyl protease DDI2 drives adaptation to proteasome inhibition in multiple myeloma.

Proteasome inhibitors, such as bortezomib, are first-line therapy against multiple myeloma (MM). Unfortunately, patients frequently become refractory to this treatment. The transcription factor NRF1 has been proposed to initiate an adaptation program that regulates proteasome levels. In the context of proteasome inhibition, the cytosolic protease DDI2 cleaves NRF1 to release an active fragment that translocates to the nucleus to promote the transcription of new proteasome subunits. However, the contribution of the DDI2-NRF1 pathway to bortezomib resistance is poorly understood. Here we show that upon prolonged bortezomib treatment, MM cells become resistant to proteasome inhibition by increasing the expression of DDI2 and consequently activation of NRF1. Furthermore, we found that many MM cells became more sensitive to proteasome impairment in the context of DDI2 deficiency. Mechanistically, we demonstrate that both the protease and the HDD domains of DDI2 are required to activate NRF1. Finally, we show that partial inhibition of the DDI2-protease domain with the antiviral drug nelfinavir increased bortezomib susceptibility in treated MM cells. Altogether, these findings define the DDI2-NRF1 pathway as an essential program contributing to proteasome inhibition responses and identifying DDI2 domains that could be targets of interest in bortezomib-treated MM patients.

Homeopathy and high dilutions in the context of water structure research: the experience in the 9thWorld Water Forum ­2022

Water plays a vital role in adapting natural and human systems in a climate change scenario. Understandinghow this fundamental element of life is organized and functioning is essentialtogather, disseminate, and advanceknowledge about water at the micro and nanostructure level. With developments in the different research areas, the International Panel on Water Structure­IPWS was created during the 8thWorld Water Forum(WWF)inBrasília,2018,to connect researchersspecialized inwater structure under aninterdisciplinary perspective.In 2020 a partnership between CIRAT ­International Center on Water and Transdisciplinarityand WATER­a Multidisciplinary Research Journalhasbegun, intendedto organize a special edition on Water and Transdisciplinarityto be launched during the 9thWWF, in Dakar, on March 24th, 2022, in response to the challenges of our century, with the aim to disclose advanced knowledge about water at different structural levels, leading to a new understanding of it.The whole project was organized by Prof. Leoni Bonamin, from UniversityPaulista, with the support of the Editor-in-Chief Prof. Gerald Pollack, from Washington University at Seattle. The focus was on the cross-over of information with technical quality and bold thinking, as shown in Figure 1 below.Homeopathy wasincludedamong the themes.Aftera multi-step process, in which manuscripts were received (after personal invitationssent to more than 200 researchers in the field)andevaluated in a two-step peer review processto ensure maximum scientific understanding, the result showed a set of innovative contributions in which authors were allowed to be bold in their projects while careful with the methodology. Theentire process was carried out voluntarily by all the actors involved.Thus, the whole editorial process was philanthropic, there were no costs for the authors, and all articles were published as open access. Authors from five countries participated in this project: Brazil, India, Italy, Russia, and the United States. Different sessions were organized according to the topics: "Opinion Article," "Hypotheses," "Water Structure," "High Dilutions," "Water and Environment," and "New Devices."Fiveof twelve articles reported results on high dilutions, including its physicochemical propertiesandthe implications on biological systems, such as plants and aquatic animals [1-4]. This initiative inspired all to envision the possibility of offering practical solutions to old problems involving different ways of water management, which couldreduce social gaps and improve the quality of life fairly and universally.

Brain-Derived Neurotrophic Factor and Psychophysiological Response in Youth Badminton Athletes During the Season.

PURPOSE: To analyze peripheral brain-derived neurotrophic factor (BDNF) levels and psychophysiological parameters in youth badminton athletes during the season and to determine the relationship between variables. METHODS: Fourteen young badminton athletes were assessed over the season (preseason, middle season, and final season). Serum BDNF (sBDNF) was determined during the preseason and final season. Sleep time, total physical activity, and time in vigorous activity were measured using an accelerometer. The fat-free mass, skeletal muscle mass, fat mass, handgrip strength, cardiorespiratory fitness (VO2max), and dietary intake were evaluated during the season. The Stroop Color and Word Test was employed to assess cognitive tasks. To evaluate the mood, the Brunel Mood Scale was used. RESULTS: There  were lower sBDNF levels (-16.3% [46.8%]; P = .007) and sleep time (final season = 5.7 [1.1] vs preseason = 6.6 [1.1] h·night-1, P = .043) during the end of the season. The total calories and carbohydrate intake decreased across the season (P < .05). Conversely, better cognitive function was found in the final season with respect to the preseason (P < .05). There were significant correlations between BDNF and VO2max only in the preseason (r = .61, P = .027), but no significant relationship was found among sBDNF and cognitive performance, sleep time, and percentage of won games. CONCLUSIONS: Youth badminton athletes decreased their sBDNF levels, sleep time, carbohydrate, and calorie intake across the season. The athletes improved in cognitive function; however, only the females improved in body composition, and the males improved their VO2max in the middle season. The sBDNF levels were positively correlated with the VO2max in the preseason, and no correlations were observed among the sBDNF and psychological parameters, sleep time, and sport performance during the season.

Effects of FFP2/N95 face mask on low- and high-load resistance exercise performance in recreational weight lifters.

COVID-19 pandemic, has led to several countries adopting the use of masks in public spaces. Mask used during physical exercise it may induce early fatigue. However, despite the results with aerobic exercise, as far as we know, no studies have been carried out on wearing a mask during resistance exercise.This randomized, crossover study verified the acute effect of an FFP2/N95 face mask on moderate- and high-load upper body resistance exercise performance in recreational weight lifters.The FFP2/N95 face mask impacted performance, evaluated with bar velocity, in the high-intensity resistance bench press exercise until movement failure but decreased oxygen saturation and increased rate of perceived effort only in the moderate-intensity exercise.

A scoping study of pulmonary paracoccidioidomycosis: severity classification based on radiographic and tomographic evaluation

The lungs have great importance in patients with paracoccidioidomycosis since they are the portal of entry for the infecting fungi, the site of quiescent foci, and one of the most frequently affected organs. Although they have been the subject of many studies with different approaches, the severity classification of the pulmonary involvement, using imaging procedures, has not been carried out yet. This study aimed to classify the active and the residual pulmonary damage using radiographic and tomographic evaluations, according to the area involved and types of lesions.

Deslocamento linear longitudinal do ventrículo esquerdo comparado ao strain longitudinal global por ressonância magnética cardiovascular / Left ventricular longitudinal linear displacement versus global longitudinal strain on cardiovascular magnetic resonance

Introdução: A função sistodiastólica do ventrículo esquerdo é prognóstica nas doenças cardiovasculares e pode ser avaliada por strain longitudinal global por meio de ecocardiografia e de ressonância magnética cardíaca. O strain longitudinal global pela ressonância magnética cardíaca exige a utilização de software de alto custo. O deslocamento linear longitudinal do ventrículo esquerdo pode ser uma alternativa simples e barata ao strain longitudinal global, porém eles não foram ainda comparados sistematicamente. Objetivo: Comparar o deslocamento linear longitudinal com o strain longitudinal global e fração de ejeção do ventrículo esquerdo em valvopatas aórticos e controles. Métodos: Incluímos 44 participantes (26 valvopatas aórticos/19 controles). O strain longitudinal global utilizou software específico (Circle Cardiovascular Imaging 42) e o deslocamento linear longitudinal apenas medidas lineares de distância entre a base e o ápex do ventrículo esquerdo, gerando deslocamento máximo, velocidade máxima no início da diástole, velocidade na diástase e a relação entre velocidade na diástase e velocidade máxima no início da diástole. Resultados: Deslocamento máximo e velocidade máxima no início da diástole correlacionaram-se com strain longitudinal global (r=0,69 e r=0,65 respectivamente) e com a fração de ejeção do ventrículo esquerdo (r=0,47 e r=0,57, p<0,001 para ambos). Deslocamento máximo e velocidade máxima no início da diástole apresentaram área sob a curva Característica de Operação do Receptor de 0,88 e 0,91 e, no melhor ponto de corte (-0,13 e 0,66), sensibilidade de 72,43% e 57,14% e especificidade 80,65% e 87,10%, respectivamente, quando comparados ao strain longitudinal global. Utilizando a fração de ejeção do ventrículo esquerdo como referência, foram obtidos 0,70 e 0,82, e, no melhor ponto de corte (-0,11 e 0,61), sensibilidade de 75,00% e 50,00% e especificidade 72,97% e 78,38%, respectivamente. Conclusão: O deslocamento linear longitudinal foi semelhante ao strain longitudinal global. O deslocamento máximo derivado do deslocamento linear longitudinal foi o melhor parâmetro na sístole, enquanto a velocidade máxima no início da diástole foi o melhor na diástole, o que possibilita a avaliação da função diastólica pela ressonância magnética cardíaca na rotina clínica de forma rápida e sem custo adicional.(AU)

Background: Left ventricular (LV) systolic diastolic function is prognostic in cardiovascular diseases and can be assessed via global longitudinal strain (GLS) on echocardiography and cardiac magnetic resonance (CMR). However, GLS by CMR requires the use of expensive software. Longitudinal linear displacement (LLD) may be a simple and inexpensive alternative to GLS, but the two have not been systematically compared. Objective: To compare LLD with GLS and LV ejection fraction (LVEF) in aortic valve disease patients and controls. Methods: We included 44 participants (26 with aortic valve disease, 19 controls). GLS was determined using CVI42 software (Circle Cardiovascular Imaging), while the LLD linear measurements of the distance between the base/apex of the LV included maximum displacement (MD), maximum velocity in early diastole (MVED), atrioventricular junction velocity in diastasis (VDS), and VDS/MVED ratio. Results: DM and MVED were correlated with GLS (r=0.69 and r=0.65, respectively) and LVEF (r=0.47 and r=0.57, p<0.001 for both). DM and MVED showed areas under the receiver operating characteristic curve (AUC) of 0.88 and 0.91, and at the best cut-off point (-0.13 and 0.66), sensitivities of 72.43% and 57.14% and specificities of 80.65% and 87.10%, respectively, compared to GLS. Using LVEF as a reference, we obtained AUC of 0.70 and 0.82, and at the best cut-off point (-0.11 and 0.61), sensitivities of 75.00% and 50.00% and specificities of 72.97% and 78.38%, respectively. Conclusion: LLD demonstrated similar performance to that of GLS. MD derived from LLD was the best parameter during systole, while MVED was the best during diastole. Our findings demonstrate the routine, quick, and inexpensive assessment of diastolic function on CMR.(AU)

Proteomic analysis of serum samples of paracoccidioidomycosis patients with severe pulmonary sequel.

BACKGROUND: Pulmonary sequelae (PS) in patients with chronic paracoccidioidomycosis (PCM) typically include pulmonary fibrosis and emphysema. Knowledge of the molecular pathways involved in PS of PCM is required for treatment and biomarker identification. METHODOLOGY/PRINCIPAL FINDINGS: This non-concurrent cohort study included 29 patients with pulmonary PCM that were followed before and after treatment. From this group, 17 patients evolved to mild/ moderate PS and 12 evolved severe PS. Sera from patients were evaluated before treatment and at clinical cure, serological cure, and apparent cure. A nanoACQUITY UPLC-Xevo QT MS system and PLGS software were used to identify serum differentially expressed proteins, data are available via ProteomeXchange with identifier PXD026906. Serum differentially expressed proteins were then categorized using Cytoscape software and the Reactome pathway database. Seventy-two differentially expressed serum proteins were identified in patients with severe PS compared with patients with mild/moderate PS. Most proteins altered in severe PS were involved in wound healing, inflammatory response, and oxygen transport pathways. Before treatment and at clinical cure, signaling proteins participating in wound healing, complement cascade, cholesterol transport and retinoid metabolism pathways were downregulated in patients with severe PS, whereas signaling proteins in gluconeogenesis and gas exchange pathways were upregulated. At serological cure, the pattern of protein expression reversed. At apparent cure pathways related with tissue repair (fibrosis) became downregulated, and pathway related oxygen transport became upregulated. Additionally, we identified 15 proteins as candidate biomarkers for severe PS. CONCLUSIONS/SIGNIFICANCE: Development of severe PS is related to increased expression of proteins involved in glycolytic pathway and oxygen exchange), indicative of the greater cellular activity and replication associated with early dysregulation of wound healing and aberrant tissue repair. Our findings provide new targets to study mechanisms of PS in PCM, as well as potential biomarkers.

Chronic capsiate supplementation increases fat-free mass and upper body strength but not the inflammatory response to resistance exercise in young untrained men: a randomized, placebo-controlled and double-blind study.

BACKGROUND: Acute capsaicinoid and capsinoid supplementation has endurance and resistance exercise benefits; however, if these short-term performance benefits translate into chronic benefits when combined with resistance training is currently unknown. This study investigated changes of chronic Capsiate supplementation on muscular adaptations, inflammatory response and performance in untrained men. METHODS: Twenty untrained men were randomized to ingest 12 mg Capsiate (CAP) or placebo in a parallel, double-blind design. Body composition and performance were measured at pre-training and after 6 weeks of resistance training. An acute resistance exercise session test was performed pre and post-intervention. Blood samples were collected at rest and post-resistance exercise to analyze Tumor necrosis factor- (TNF-), Soluble TNF- receptor (sTNF-r), Interleukin-6 (IL-6) and Interleukin-10 (IL-10). RESULTS: Exercise and CAP supplementation increased fat-free mass in comparison to baseline by 1.5 kg (P < 0.001), however, the majority of the increase (1.0 kg) resulted from an increase in total body water. The CAP change scores for fat-free mass were significantly greater in comparison to the placebo (CAP ∆%= 2.1 ± 1.8 %, PLA ∆%= 0.7 ± 1.3 %, P = 0.043) and there was a significant difference between groups in the bench press exercise (P = 0.034) with greater upper body strength change score for CAP (∆%= 13.4 ± 9.1 %) compared to placebo (∆%= 5.8 ± 5.2 %), P = 0.041. CAP had no effect on lower body strength and no supplementation interactions were observed for all cytokines in response to acute resistance exercise (P > 0.05). CONCLUSION: Chronic Capsiate supplementation combined with resistance training during short period (6 weeks) increased fat-free mass and upper body strength but not inflammatory response and performance in young untrained men.

Feature-tracking cardiac magnetic resonance left ventricular global longitudinal strain improves 6 months after kidney transplantation associated with reverse remodeling, not myocardial tissue characteristics.

To determine whether left ventricular (LV) global longitudinal strain (GLS) measured by feature-tracking (FT) cardiac magnetic resonance (CMR) improves after kidney transplantation (KT) and to analyze associations between LV GLS, reverse remodeling and myocardial tissue characteristics. This is a prospective single-center cohort study of kidney transplant recipients who underwent two CMR examinations in a 3T scanner, including cines, tagging, T1 and T2 mapping. The baseline exam was done up to 10 days after transplantation and the follow-up after 6 months. Age and sex-matched healthy controls were also studied for comparison. A total of 44 patients [mean age 50 ± 11 years-old, 27 (61.4%) male] completed the two CMR exams. LV GLS improved from - 13.4% ± 3.0 at baseline to - 15.2% ± 2.7 at follow-up (p < 0.001), but remained impaired when compared with controls (- 17.7% ± 1.5, p = 0.007). We observed significant correlation between improvement in LV GLS with reductions of left ventricular mass index (r = 0.356, p = 0.018). Improvement in LV GLS paralleled improvements in LV stroke volume index (r = - 0.429, p = 0.004), ejection fraction (r = - 0.408, p = 0.006), global circumferential strain (r = 0.420, p = 0.004) and global radial strain (r = - 0.530, p = 0.002). There were no significant correlations between LV GLS, native T1 or T2 measurements (p > 0.05). In this study, we demonstrated that LV GLS measured by FT-CMR improves 6 months after KT in association with reverse remodeling, but not native T1 or T2 measurements.