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1.
Viruses ; 8(5)2016 05 13.
Artigo em Inglês | MEDLINE | ID: mdl-27187446

RESUMO

The identification of host cellular genes that act as either proviral or antiviral factors has been aided by the development of an increasingly large number of high-throughput screening approaches. Here, we review recent advances in which these new technologies have been used to interrogate host genes for the ability to impact bunyavirus infection, both in terms of technical advances as well as a summary of biological insights gained from these studies.


Assuntos
Infecções por Bunyaviridae/imunologia , Infecções por Bunyaviridae/patologia , Interações Hospedeiro-Patógeno , Orthobunyavirus/patogenicidade , Testes Genéticos/métodos , Ensaios de Triagem em Larga Escala , Humanos
2.
AIDS Res Hum Retroviruses ; 32(7): 705-17, 2016 07.
Artigo em Inglês | MEDLINE | ID: mdl-26847431

RESUMO

Platelet factor 4 (PF4) has been recently shown to inhibit infection by a broad range of human immunodeficiency virus type 1 (HIV-1) isolates in vitro. We found that the inhibitory effects of PF4 are limited to a defined concentration range where PF4 exists largely in a monomeric state. Under these conditions, PF4 bound the HIV-1 envelope protein and inhibited HIV-1 attachment to the cell surface. However, as concentrations increased to the point where PF4 exists largely in tetrameric or higher-order forms, viral infection in vitro was enhanced. Enhancement could be inhibited by mutations in PF4 that shift the oligomeric equilibrium toward the monomeric state, or by using soluble glycosaminoglycans (GAGs) to which tetrameric PF4 avidly binds. We conclude that at physiologically relevant concentrations, oligomeric PF4 enhances infection by HIV-1 by interacting with the viral envelope protein as well as cell surface GAGs, enhancing virus attachment to the cell surface. This effect was not specific to HIV-1, as enhancement was seen with some but not all other viruses tested. The biphasic effects of PF4 on HIV-1 infection suggest that native PF4 will not be a useful antiviral agent and that PF4 could contribute to the hematologic abnormalities commonly seen in HIV-infected individuals by enhancing virus infection in the bone marrow.


Assuntos
HIV-1/fisiologia , Interações Hospedeiro-Patógeno , Fator Plaquetário 4/metabolismo , Ligação Viral , Produtos do Gene env do Vírus da Imunodeficiência Humana/metabolismo , Humanos , Ligação Proteica
3.
J Virol ; 90(3): 1414-23, 2016 02 01.
Artigo em Inglês | MEDLINE | ID: mdl-26581979

RESUMO

UNLABELLED: Rift Valley fever virus (RVFV) causes recurrent insect-borne epizootics throughout the African continent, and infection of humans can lead to a lethal hemorrhagic fever syndrome. Deep mutagenesis of haploid human cells was used to identify host factors required for RVFV infection. This screen identified a suite of enzymes involved in glycosaminoglycan (GAG) biogenesis and transport, including several components of the cis-oligomeric Golgi (COG) complex, one of the central components of Golgi complex trafficking. In addition, disruption of PTAR1 led to RVFV resistance as well as reduced heparan sulfate surface levels, consistent with recent observations that PTAR1-deficient cells exhibit altered Golgi complex morphology and glycosylation defects. A variety of biochemical and genetic approaches were utilized to show that both pathogenic and attenuated RVFV strains require GAGs for efficient infection on some, but not all, cell types, with the block to infection being at the level of virion attachment. Examination of other members of the Bunyaviridae family for GAG-dependent infection suggested that the interaction with GAGs is not universal among bunyaviruses, indicating that these viruses, as well as RVFV on certain cell types, employ additional unidentified virion attachment factors and/or receptors. IMPORTANCE: Rift Valley fever virus (RVFV) is an emerging pathogen that can cause severe disease in humans and animals. Epizootics among livestock populations lead to high mortality rates and can be economically devastating. Human epidemics of Rift Valley fever, often initiated by contact with infected animals, are characterized by a febrile disease that sometimes leads to encephalitis or hemorrhagic fever. The global burden of the pathogen is increasing because it has recently disseminated beyond Africa, which is of particular concern because the virus can be transmitted by widely distributed mosquito species. There are no FDA-licensed vaccines or antiviral agents with activity against RVFV, and details of its life cycle and interaction with host cells are not well characterized. We used the power of genetic screening in human cells and found that RVFV utilizes glycosaminoglycans to attach to host cells. This furthers our understanding of the virus and informs the development of antiviral therapeutics.


Assuntos
Proteoglicanas de Heparan Sulfato/metabolismo , Vírus da Febre do Vale do Rift/fisiologia , Ligação Viral , Linhagem Celular , Testes Genéticos , Proteoglicanas de Heparan Sulfato/genética , Humanos , Mutagênese
4.
J Exp Med ; 211(4): 653-68, 2014 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-24638169

RESUMO

Microbial infection triggers assembly of inflammasome complexes that promote caspase-1-dependent antimicrobial responses. Inflammasome assembly is mediated by members of the nucleotide binding domain leucine-rich repeat (NLR) protein family that respond to cytosolic bacterial products or disruption of cellular processes. Flagellin injected into host cells by invading Salmonella induces inflammasome activation through NLRC4, whereas NLRP3 is required for inflammasome activation in response to multiple stimuli, including microbial infection, tissue damage, and metabolic dysregulation, through mechanisms that remain poorly understood. During systemic infection, Salmonella avoids NLRC4 inflammasome activation by down-regulating flagellin expression. Macrophages exhibit delayed NLRP3 inflammasome activation after Salmonella infection, suggesting that Salmonella may evade or prevent the rapid activation of the NLRP3 inflammasome. We therefore screened a Salmonella Typhimurium transposon library to identify bacterial factors that limit NLRP3 inflammasome activation. Surprisingly, absence of the Salmonella TCA enzyme aconitase induced rapid NLRP3 inflammasome activation. This inflammasome activation correlated with elevated levels of bacterial citrate, and required mitochondrial reactive oxygen species and bacterial citrate synthase. Importantly, Salmonella lacking aconitase displayed NLRP3- and caspase-1/11-dependent attenuation of virulence, and induced elevated serum IL-18 in wild-type mice. Together, our data link Salmonella genes controlling oxidative metabolism to inflammasome activation and suggest that NLRP3 inflammasome evasion promotes systemic Salmonella virulence.


Assuntos
Proteínas de Transporte/metabolismo , Evasão da Resposta Imune/imunologia , Inflamassomos/metabolismo , Salmonella typhimurium/metabolismo , Aconitato Hidratase/metabolismo , Animais , Proteínas Reguladoras de Apoptose/metabolismo , Sistemas de Secreção Bacterianos , Proteínas de Ligação ao Cálcio/metabolismo , Citrato (si)-Sintase/metabolismo , Ciclo do Ácido Cítrico , Genes Bacterianos/genética , Imunidade , Isocitrato Desidrogenase/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Mitocôndrias/metabolismo , Mutação/genética , Proteína 3 que Contém Domínio de Pirina da Família NLR , Oxirredução , Espécies Reativas de Oxigênio/metabolismo , Salmonelose Animal/imunologia , Salmonelose Animal/patologia , Salmonella typhimurium/enzimologia , Salmonella typhimurium/genética , Salmonella typhimurium/imunologia
5.
PLoS Pathog ; 10(2): e1003911, 2014 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-24516383

RESUMO

The Bunyaviridae comprise a large family of RNA viruses with worldwide distribution and includes the pathogenic New World hantavirus, Andes virus (ANDV). Host factors needed for hantavirus entry remain largely enigmatic and therapeutics are unavailable. To identify cellular requirements for ANDV infection, we performed two parallel genetic screens. Analysis of a large library of insertionally mutagenized human haploid cells and a siRNA genomic screen converged on components (SREBP-2, SCAP, S1P and S2P) of the sterol regulatory pathway as critically important for infection by ANDV. The significance of this pathway was confirmed using functionally deficient cells, TALEN-mediated gene disruption, RNA interference and pharmacologic inhibition. Disruption of sterol regulatory complex function impaired ANDV internalization without affecting virus binding. Pharmacologic manipulation of cholesterol levels demonstrated that ANDV entry is sensitive to changes in cellular cholesterol and raises the possibility that clinically approved regulators of sterol synthesis may prove useful for combating ANDV infection.


Assuntos
Colesterol/metabolismo , Infecções por Hantavirus/metabolismo , Interações Hospedeiro-Parasita/fisiologia , Orthohantavírus/patogenicidade , Internalização do Vírus , Linhagem Celular , Citometria de Fluxo , Humanos , Microscopia Confocal , Mutagênese Sítio-Dirigida , Reação em Cadeia da Polimerase , Transdução de Sinais/fisiologia , Esteróis/metabolismo , Transdução Genética , Replicação Viral/fisiologia
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