Bovine monoclonal antibodies specific for bovine herpesvirus-1 glycoprotein gIII.

Spleen cells from a calf immunized with bovine herpesvirus-1 (BHV-1) were fused with the nonsecreting murine cell line SP2/0. Several bovine-murine hybridomas secreting bovine immunoglobulins were stabilized. Of these, 9 hybridomas secreted bovine monoclonal antibodies that specifically bound to BHV-1 in a radioimmunoassay. Two of these monoclonal antibodies reacted specifically with BHV-1 in an indirect fluorescent antibody test and immunoprecipitated a BHV-1 glycoprotein with molecular mass of 97 kilodaltons.

Effects of training in use of executive strategies on a verbal memory problem resulting from closed head injury.

This case study reports attempts to improve the recall performance of an adolescent (GC) who had suffered a closed-head injury. GC had a very limited range of ways of processing both spoken and written information and showed significant recall problems. Initial training in the use of strategies for list learning resulted in improvement in paired-associate recall but showed that initiation and use of the newly learned strategies would not occur without prompting. Executive strategy training was provided to improve GC's ability to identify a memory problem and to initiate a general plan for dealing with that problem. This training involved consideration of task analysis, strategy selection and initiation, and monitoring of strategy use. Evidence of long-term maintenance of improvement in level of recall on both paired-associate and free recall tests was noted following the executive strategy training.

Transient neonatal tyrosinaemia.

Children who had presented with transient neonatal tyrosinaemia (TNT) were compared with a group of unaffected controls at 7-9 years of age. A comprehensive psychometric assessment revealed significant differences between the groups in adaptive behaviour, psycholinguistic abilities, and speed of learning. In nearly all components of the tests used, higher levels of TNT were associated with lower levels of performance. This study demonstrates that TNT, a condition commonly regarded as benign in the short term, has long-term effects which may be detrimental to the child in school.

Incarceration of a section of small intestine by remnants of the ductus deferens in steers.

A 13-month-old Charolais steer was examined because of anorexia and weight loss of 1 week's duration. Initially, the steer passed tarry feces for 2 days, but no feces were passed for 4 days before examination. Palpation per rectum disclosed moderate distention of the small intestine and dark tenacious mucus in the rectum. Standing celiotomy was performed through the right paralumbar fossa, and a 60-cm segment of small intestine was found to be entrapped by a fibrous band between the caudal abdominal wall and the pelvic inlet. The band was transected and the intestine was freed. The intestine was judged to be viable and the abdomen was closed. Histologically, the fibrous band consisted of ductus deferens and adnexa; the intestine had become incarcerated through a rent in the mesoductus. Small intestine entrapment through a rent in the right mesoductus was diagnosed in 20 additional steers over a 10-year period. In all cases, the steers had been castrated when weighing more than 100 kg, by manual traction on the testicle and spermatic cord through a scrotal incision. Alternate means of castration that prevent disruption of the abdominal mesoductus may prevent development of this condition.