RESUMO
BACKGROUND: Dermorphin, a hepta-peptide with potent analgesic properties, is classified as a doping agent in equine racing. Since its discovery, a number of biologically active structural analogs have been synthesized and made commercially available so there is a need for reliable methods of detection. METHODOLOGY/RESULTS: A sensitive detection method was developed for dermorphin and six analogs in equine urine. Peptide enrichment was achieved using weak cation exchange with subsequent separation and detection by nano-UHPLC-MS/MS. Method validation parameters included: specificity, linearity (5-10000 pg/ml), recovery (58-93%), intra and inter-assay repeatability, LOD (5-50 pg/ml) and matrix effects. CONCLUSION: The presented method will facilitate the control of the abuse of dermorphin and selected analogs in equine sports.
Assuntos
Analgésicos Opioides/urina , Cromatografia Líquida de Alta Pressão/veterinária , Cavalos/urina , Peptídeos Opioides/urina , Detecção do Abuso de Substâncias/veterinária , Espectrometria de Massas em Tandem/veterinária , Analgésicos Opioides/análise , Animais , Cromatografia Líquida de Alta Pressão/métodos , Limite de Detecção , Peptídeos Opioides/análise , Detecção do Abuso de Substâncias/métodos , Espectrometria de Massas em Tandem/métodosRESUMO
Interprofessional collaboration is critical to the provision of safe patient care and provider satisfaction. Collaboration is an active process that can help maximize positive patient outcomes. Three academic institutions implemented collaborative processes as part of their perinatal patient safety initiatives based on anecdotal experiences and safety culture surveys that demonstrated positive outcomes. Reliable tools and additional research are needed to measure the extent and impact of collaboration on patient outcomes in perinatal care.
