Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Inflamm Res ; 57(4): 145-50, 2008 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-18368290

RESUMO

OBJECTIVE: Evaluate the Monocyte Locomotion Inhibitory Factor (MLIF) effect upon the expression of genes encoding human cytokines, receptors and related factors in the human cell line U-937. MLIF (Met-Gln-Cys-Asn-Ser) is an anti-inflammatory pentapeptide produced by Entamoeba histolytica that inhibits many human monocyte functions. MATERIAL AND METHODS: U-937 cell line cultured (24 hrs/RPMI). RNA extracted by Trizol method. 385 genes were analyzed on microarray membranes, complement by real-time RT-PCR and protein expression of some affected genes. RESULTS: MLIF had a preferentially inhibitory effect on gene expression; four genes were over-expressed and 13 underexpressed in MILF vs. simple medium - constitutive expression. Three genes are over-expressed and 19 under-expressed in MLIF/PMA vs. PMA - induced expression. CONCLUSIONS: Many modified genes are products regulated by the Nuclear Factor-kappaB and Mitogen Activated Protein Kinase pathways, suggesting MLIF involvement with these two major pathways for the modulation of the inflammation and immune responses.


Assuntos
Citocinas/metabolismo , Entamoeba histolytica/metabolismo , Expressão Gênica/efeitos dos fármacos , Monócitos/metabolismo , Oligopeptídeos/metabolismo , Oligopeptídeos/farmacologia , Animais , Citocinas/genética , Perfilação da Expressão Gênica , Humanos , Inflamação/metabolismo , Quinases de Proteína Quinase Ativadas por Mitógeno/metabolismo , Modelos Biológicos , Monócitos/citologia , NF-kappa B/metabolismo , Análise de Sequência com Séries de Oligonucleotídeos , Células U937
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...