Three-dimensional analysis of the accuracy of conventional and completely digital interocclusal registration methods.

STATEMENT OF PROBLEM: Compared with the frequent investigations into the accuracy of digital intraoral scans, studies analyzing digital determinations of jaw relationships based on intraoral scans are scarce. PURPOSE: The purpose of this in vitro study was to present an optical 3-dimensional method for analyzing deviations in static occlusion and to compare the accuracy of conventional and digital interocclusal registrations. MATERIAL AND METHODS: A Frasaco jaw model was duplicated, articulated, and scanned with a high-precision industrial scanner, and the data were stored in a virtual standard tessellation language (STL) format, which served as the reference model. Fifteen paired mandibular and maxillary models were scanned with a digital intraoral scanner in the completely digital workflow (IOS group). Forty-five paired gypsum casts were poured from polyvinyl siloxane (PVS) impressions and associated with 2 different PVS registration materials. These casts were digitized with a laboratory scanner and grouped as follows (n=15/group): PVS group, conventional Futar D interocclusal record; sPVS group, conventional Futar Scan interocclusal record; and the AIR group, partially digital antagonist scan of the Futar Scan interocclusal record. The axes (X, Y, Z, and XYZ) of each paired model were aligned to those of the reference model by 3-dimensional superimposition, and deviations were calculated. To determine the ideal zero position, a best-fit over the mandibular teeth between the reference model and the actual model was estimated. Next, a second best-fit was determined between the maxillary models to determine the actual position of the mandibular model. The different registration methods were compared with the Mann-Whitney U test (α=.05). RESULTS: In the IOS group, the interocclusal registration caused a mandibular deviation of 0.05 ±0.04 mm (mean ±standard deviation). This fit was better than those of conventional registrations with inserted interocclusal registration materials (PVS group and sPVS group), which caused mean z-axis deviations of 0.41 ±0.46 mm and 0.44 ±0.32 mm (P<.001), with the deviations leading to elevation of the mandibular model. The partially digital workflow with a scannable registration material (AIR group) showed significantly larger deviations in the x-axis (0.15 ±0.08 mm; P=.042) compared with the IOS group. No significant difference was observed in the total deviation between the IOS and the AIR groups. Both groups showed significantly smaller deviations than the conventional registration methods (P<.001 for the IOS group and P=.023 for the AIR group). CONCLUSIONS: In comparison with maxillary and mandibular alignment using conventional interocclusal registration materials, digital interocclusal registrations showed greater accuracy in evaluating complete jaw models and can be recommended for clinical use. Additionally, the partially digital workflow with an antagonist scan of the interocclusal record provided acceptable results.

Finding Possible Weakness in the Runoff Simulation Experiments to Assess Rill Erosion Changes without Non-Intermittent Surveying Capabilities.

The Terrestrial Photogrammetry Scanner (TEPHOS) offers the possibility to precisely monitor linear erosion features using the Structure from Motion (SfM) technique. This is a static, multi-camera array and dynamically moves the digital videoframe camera designed to obtain 3-D models of rills before and after the runoff experiments. The main goals were to (1) obtain better insight into the rills; (2) reduce the technical gaps generated during the runoff experiments using only one camera; (3) enable the visual location of eroded, transported and accumulated material. In this study, we obtained a mean error for all pictures reaching up to 0.00433 pixels and every single one of them was under 0.15 pixel. So, we obtained an error of about 1/10th of the maximum possible resolution. A conservative value for the overall accuracy was one pixel, which means that, in our case, the accuracy was 0.0625 mm. The point density, in our example, reached 29,484,888 pts/m². It became possible to get a glimpse of the hotspots of sidewall failure and rill-bed incision. We conclude that the combination of both approaches-rill experiment and 3D models-will make easy under laboratory conditions to describe the soil erosion processes accurately in a mathematical-physical way.

Rainfall-simulated quantification of initial soil erosion processes in sloping and poorly maintained terraced vineyards - Key issues for sustainable management systems.

In the context of the Sustainable Development Goals (SDG), understanding landscape evolution is essential to design long-term management plans. In agricultural fields, such as the vineyards on steep slopes, the terraces offer one of the most important morphological changes. However, it is not clear if the poorly managed agricultural terraces are optimal to reduce soil erosion and overland flow, although the trafficability is improved. Therefore, the main aim of this research is to compare the differences between initial soil erosion processes on poorly managed terraced vineyards and sloping vineyards at the pedon scale, considering the key role of the SSC (Soil Surface Components). To achieve this goal, twenty-six rainfall simulations were performed, considering the inclination, vegetation and stone covers, and surface roughness. Our research was carried out in the sloping vineyards (>20°) of the Almáchar municipality, in the Montes de Málaga (Spain). Those vineyards are characterized by bare soils, low organic matter and high rock fragment contents. Our results showed that higher soil losses (42.2 g m-2vs 9.4 g m-2) and runoff (4.9 l m-2vs 1.6 l m-2) were detected in the plots of the poorly managed terraced vineyard than in the sloping one. Moreover, the time to runoff generation was lower in the poorly conserved terraces (232 s) than in the sloping vineyard (679 s), showing a faster saturation capacity. The SSC considered as the key factors were the reduction of the stone cover and an increase of roughness. As a conclusion, we confirm that the imminent transformation from sloping vineyards into terraced fields could lead several land degradation processes if a poor management is carried out, and no control measures are applied during the process, such as the conservation of stone walls or vegetation cover above the embankment, which is not in compliance with the SDG.

Prognostic Value of Homotypic Cell Internalization by Nonprofessional Phagocytic Cancer Cells.

BACKGROUND: In this study, we investigated the prognostic role of homotypic tumor cell cannibalism in different cancer types. METHODS: The phenomenon of one cell being internalized into another, which we refer to as "cell-in-cell event," was assessed in 416 cases from five head and neck cancer cohorts, as well as one anal and one rectal cancer cohort. The samples were processed into tissue microarrays and immunohistochemically stained for E-cadherin and cleaved caspase-3 to visualize cell membranes and apoptotic cell death. RESULTS: Cell-in-cell events were found in all of the cohorts. The frequency ranged from 0.7 to 17.3 cell-in-cell events per mm(2). Hardly any apoptotic cells were found within the cell-in-cell structures, although apoptotic cell rates were about 1.6 to two times as high as cell-in-cell rates of the same tissue sample. High numbers of cell-in-cell events showed adverse effects on patients' survival in the head and neck and in the rectal cancer cohorts. In multivariate analysis, high frequency was an adverse prognostic factor for overall survival in patients with head and neck cancer (p = 0.008). CONCLUSION: Cell-in-cell events were found to predict patient outcomes in various types of cancer better than apoptosis and proliferation and might therefore be used to guide treatment strategies.

Applicability of different hydraulic parameters to describe soil detachment in eroding rills.

This study presents the comparison of experimental results with assumptions used in numerical models. The aim of the field experiments is to test the linear relationship between different hydraulic parameters and soil detachment. For example correlations between shear stress, unit length shear force, stream power, unit stream power and effective stream power and the detachment rate does not reveal a single parameter which consistently displays the best correlation. More importantly, the best fit does not only vary from one experiment to another, but even between distinct measurement points. Different processes in rill erosion are responsible for the changing correlations. However, not all these procedures are considered in soil erosion models. Hence, hydraulic parameters alone are not sufficient to predict detachment rates. They predict the fluvial incising in the rill's bottom, but the main sediment sources are not considered sufficiently in its equations. The results of this study show that there is still a lack of understanding of the physical processes underlying soil erosion. Exerted forces, soil stability and its expression, the abstraction of the detachment and transport processes in shallow flowing water remain still subject of unclear description and dependence.

Sortase-mediated assembly and surface topology of adhesive pneumococcal pili.

The rlrA genetic islet encodes an extracellular pilus in the Gram-positive pathogen Streptococcus pneumoniae. Of the three genes for structural subunits, rrgB encodes the major pilin, while rrgA and rrgC encode ancillary pilin subunits decorating the pilus shaft and tip. Deletion of all three pilus-associated sortase genes, srtB, srtC and srtD, completely prevents pilus biogenesis. Expression of srtB alone is sufficient to covalently associate RrgB subunits to one another as well as linking the RrgA adhesin and the RrgC subunit into the polymer. The active-site cysteine residue of SrtB (Cys 177) is crucial for incorporating RrgC, even when the two other sortase genes are expressed. SrtC is redundant to SrtB in permitting RrgB polymerization, and in linking RrgA to the RrgB filament, but SrtC is insufficient to incorporate RrgC. In contrast, expression of srtD alone fails to mediate RrgB polymerization, and a srtD mutant assembles heterotrimeric pilus indistinguishable from wild type. Topological studies demonstrate that pilus antigens are localized to symmetric foci at the cell surface in the presence of all three sortases. This symmetric focal presentation is abrogated in the absence of either srtB or srtD, while deletion of srtC had no effect. In addition, strains expressing srtB alone or srtC alone also displayed disrupted antigen localization, despite polymerizing subunits. Our data suggest that both SrtB and SrtC act as pilus subunit polymerases, with SrtB processing all three pilus subunit proteins, while SrtC only RrgB and RrgA. In contrast, SrtD does not act as a pilus subunit polymerase, but instead is required for wild-type focal presentation of the pilus at the cell surface.