Gut Microbiome Composition in Lynch Syndrome With and Without History of Colorectal Neoplasia and Non-Lynch Controls.

BACKGROUND: While Lynch syndrome (LS) is a highly penetrant colorectal cancer (CRC) syndrome, there is considerable variation in penetrance; few studies have investigated the association between microbiome and CRC risk in LS. We analyzed the microbiome composition among individuals with LS with and without personal history of colorectal neoplasia (CRN) and non-LS controls. METHODS: We sequenced the V4 region of the 16S rRNA gene from the stool of 46 individuals with LS and 53 individuals without LS. We characterized within community and in between community microbiome variation, compared taxon abundance, and built machine learning models to investigate the differences in microbiome. RESULTS: There was no difference within or between community variations among LS groups, but there was a statistically significant difference in both within and between community variation comparing LS to non-LS. Streptococcus and Actinomyces were differentially enriched in LS-CRC compared to LS-without CRN. There were numerous differences in taxa abundance comparing LS to non-LS; notably, Veillonella was enriched and Faecalibacterium and Romboutsia were depleted in LS. Finally, machine learning models classifying LS from non-LS controls and LS-CRC from LS-without CRN performed moderately well. CONCLUSIONS: Differences in microbiome composition between LS and non-LS may suggest a microbiome pattern unique to LS formed by underlying differences in epithelial biology and immunology. We found specific taxa differences among LS groups, which may be due to underlying anatomy. Larger prospective studies following for CRN diagnosis and microbiome composition changes are needed to determine if microbiome composition contributes to CRN development in patients with LS.

Differences in erythrocyte phospholipid membrane long-chain polyunsaturated fatty acids and the prevalence of fatty acid desaturase genotype among African Americans and European Americans.

Numerous studies have reported an association between genetic variants in fatty acid desaturases (FADS1 and FADS2) and plasma or erythrocyte long chain polyunsaturated fatty acid (PUFA) levels. Increased levels of n-6 PUFAs have been associated with inflammation and several chronic diseases, including diabetes and cancer. We hypothesized that genetic variants of FADS that more efficiently convert precursor n-6 PUFA to arachidonic acid (AA) may explain the higher burden of chronic diseases observed in African Americans. To test this hypothesis, we measured the level of n-6 and n-3 PUFAs in erythrocyte membrane phospholipids and genotyped the rs174537 FADS variants associated with higher AA conversion among African American and European American populations. We included data from 1,733 individuals who participated in the Tennessee Colorectal Polyp Study, a large colonoscopy-based case-control study. Erythrocyte membrane PUFA percentages were measured using gas chromatography. Generalized linear models were used to estimate association of race and genotype on erythrocyte phospholipid membrane PUFA levels while controlling for self-reported dietary intake. We found that African Americans have higher levels of AA and a higher prevalence of GG allele compared to whites, 81% vs 43%, respectively. Homozygous GG genotype was negatively associated with precursor PUFAs (linoleic [LA], di-homo-γ-linolenic [DGLA]), positively associated with both product PUFA (AA, docosahexaenoic acid [DHA]), product to precursor ratio (AA to DGLA), an indirect measure of FADs efficiency and increased urinary isoprostane F2 (F2-IsoP) and isoprostane F3 (F3-IsoP), markers of oxidative stress. Increased consumption of n-6 PUFA and LA resulting in increased AA and subsequent inflammation may be fueling increased prevalence of chronic diseases especially in African descent.

A new role for Women Health Volunteers in urban Islamic Republic of Iran.

An action research project was carried out by a team from the National Public Health Management Centre in Tabriz, Iran to test the following hypothesis: Health Volunteers are more able to support health improvements by focusing on community participation and empowerment through facilitating communities to define and solve their own problems than by only providing information on health problems. Training on participatory approaches was given to Women Health Volunteers (WHV) in a pilot area. The results gave evidence that local people could identify and act upon their own health needs and request more information from professionals to improve their own health. Further research is needed however to assess how the pilot can be scaled up and how initial enthusiasm can be sustained.

A new role for Women Health Volunteers in urban Islamic Republic of Iran

An action research project was carried out by a team from the National Public Health Management Centre in Tabriz, Iran to test the following hypothesis: Health Volunteers are more able to support health improvements by focusing on community participation and empowerment through facilitating communities to define and solve their own problems than by only providing information on health problems. Training on participatory approaches was given to Women Health Volunteers [WHV] in a pilot area. The results gave evidence that local people could identify and act upon their own health needs and request more information from professionals to improve their own health. Further research is needed however to assess how the pilot can be scaled up and how initial enthusiasm can be sustained

Learning by doing: teaching qualitative methods to health care personnel.

PURPOSE: We describe and assess the teaching of qualitative methods to postgraduate students using a case study from the Centre of International Child Health, Institute of Child Health, London, which trains mainly health personnel with developing country experience. As the majority of these students are practitioners rather than academics, the teaching focuses on combining theory with practice. We then analyse the results of the assessment of students about this approach and examine lessons learned from this experience. APPROACH: We present the format of a two-week course and the evaluation of this course by the students of the past four years. We describe the process of conducting a learning-by-doing course, giving the day-to-day details of how the course is conducted. We also give examples of how this teaching is done. RESULTS: One indicator of the value of this course is its increasing popularity over the past three years. In 1997-1998, 11 students out of 20 took the course. In 1998-1999, 16 students out of 21 opted for this qualitative module. In 1999-2000, 12 students out of 17 chose this module. DISCUSSION: The lessons learned from this experience include challenges in how to present the teaching within the available time and having realistic expectations concerning course outcomes. We argue that a learning-by-doing approach accomplishes the objectives of having students recognize the value of these methods for health systems research and giving them skills needed to use these methods.

Ten best readings on community participation and health.

This article reviews, in the opinion of the author, the 10 most influential reading on community participation and health development. The introduction notes that some of the articles do not address health directly but still do bring crucial interpretations to the topic. All articles view community participation as an intervention by which the lives of people, particularly the poor and marginalised can be improved. In addition, they all address the issue of the value of participation to equity and sustainability. The article considers the readings under four heading: concepts and theory; advocacy; critiques and case studies. It highlights the important contributions each reading makes to the understanding of participation in the wider context of health and health development. In conclusion, the article argues that participation has not met the objectives of planners and professionals, in good part, because it is questionable as to whether viewing participation as an intervention enables them to make correct assessments of its contribution to development. The bottom line is that participation is always about power and control, an issue planners and professionals do not want explicitly to address.

Measuring participation: its use as a managerial tool for district health planners based on a case study in Tanzania.

While community participation has become widely practised there has been little systematic assessment of the processes involved. A framework has been developed to measure participation on a number of dimensions and this article applies this framework to a project in Tanzania. The framework helps to systematically assess the nature and extent of community participation, but a number of problems were identified as well. Suggestions are made as how to improve upon the framework and make it a useful tool for managers.

Paradigms lost: toward a new understanding of community participation in health programmes.

Community participation has been a critical part of health programmes, particularly since the acceptance of primary health care as the health policy of the member states of the World Health Organisation. However, it has rarely met the expectations of health planners/professionals. This paper argues that the reason for this failure is that community participation has been conceived in a paradigm which views community participation as a magic bullet to solve problems rooted both in health and political power. For this reason, it is necessary to use a different paradigm which views community participation as an iterative learning process allowing for a more eclectic approach to be taken. Viewing community participation in this way will enable more realistic expectations to be made. Community participation in disease control programmes focusing on community health workers is used as an example to show the limitations of the old paradigm. Participatory rapid appraisal is used to illustrate the new.

The pursuit of equity: a health sector case study from Vietnam.

The health care system in Vietnam has long been cited as an example of primary health care that has worked well. The achievements of the system during the past decades have indeed been impressive, but the changing economic situation in Vietnam has consequences for all public sector activities, including health care. Liberalization of economic policies has encouraged private medical practice and free trade in medicines and drugs, while financial support for the state health system is decreasing. Equity has always been an important goal for the vietnamese health system, but it becomes harder and harder to realize under the new conditions of financing. The restrictions in centralized planning and funding brought about by recent changes also reveal weak points in the system, from planning to training to management at the different levels. This situation is discussed and issues concerning policy, legislation and human resources are highlighted in terms of their effect on equity.

Towards the measurement of community participation.

A description is given of the initial stages in the development of a methodology for assessing community participation in health schemes. A study in Nepal indicates that the approach adopted will be useful to planners who wish to evaluate involvement in primary care programmes.

PIP: Activities, rather then processes, have been looked at in measuring community participation in health programs. However, many times planners need information about the processes that influence change. 200 case studies were gone over and things that influence community participation were analyzed. They are: 1) leadership; 2) needs assessment; 3) organization; 4) management; and 5) resource mobilization. Community participation is a social process in which groups with shared needs living in a certain geographical area actively undertake needs identification and "take" decisions and set up mechanisms to meet them. Questions can be asked about the breadth of participation. Diagrams are given for assessing community participation, baseline assessment of health program, and changes between 1st and 2nd assessments. This was tested in Nepal. The national health system is set up according to the district health care approach. The situation was discussed with various people. A ranking scale for process indicators of community participation is shown. It was expected that the assessment could be used to describe changes in participation from the start of the program. However, many people could not remember the previous period. Therefore, this will be used as a 1st assessment.

Primary health care: on measuring participation.

This paper considers the problems of finding measurements for the two major principles of primary health care (PHC), equity and participation. Although both are of equal importance, the authors concentrate on the assessment of participation. A methodology is put forward to define indicators for participation in health care programmes as how wide participation is on a continuum developed for each of the five factors which influence community participation. These factors are: needs assessment leadership, organisation, resource mobilisation and management. By plotting a mark on a continuum which is defined as wide and narrow at the extremes and is connected with all other marks in a spoke arrangement, it is possible to describe a baseline for participation in any specific health programme. This baseline can be used to compare the same programme at a different point in time, to compare observations by different evaluators, and/or to compare perceptions of different participants in the same programmes. A case study provides an example of how the indicators might be used. These indicators focus on the breadth of participation and not its potential social impact, an area which is recognised to be critical for future research.

Why health improves: defining the issues concerning 'comprehensive primary health care' and 'selective primary health care'.

What is the impact of technology on improving the life situations of people, especially the poor? How is this impact analyzed in terms of health improvements? These questions are paramount in the minds of health planners as they pursue national policies of primary health care, a policy popularized by the World Health Organization (WHO) and the United Nations Children's Fund (UNICEF) and accepted by over 150 governments at Alma Ata in 1978. The purpose of this paper is to explore these questions in depth. It begins by giving the background to the debate, then examines the origins of two concepts which have dominated the field, those of 'primary health care' and 'selective primary health care.' On this basis it suggests areas of differences in the two concepts and discusses the policy and practical implications of confusing the two approaches. The paper suggests that the differences are firstly who controls the outcome of technological interventions and the perceived time frame in which plans can be carried out.

Primary health care in Southeast Asia: attitudes about community participation in community health programmes.

Although community participation in health has become a major plank in WHO's Primary Health Care platform, comparatively little concrete programme data has been collected which helps to define its potentials and problems. In an effort to expand knowledge in this area, a study of three Church-related community health programmes in Southeast Asia was undertaken. All three programmes have the stated goal of 'having the community take responsibility for its own health care' and as a concrete step in this direction, have developed training programmes for community health workers (CHWs). Starting about the same time, 1973-1975, they provide data for comparative examination of the development of community participation. As part of the study, a questionnaire was designed to elicit information from three categories of programme participants (the medical professionals, the community development workers and the CHWs) in community health programmes. It sought to discover their attitudes about the objectives of community health programmes; impact and measurements of success of these programmes; the role of health services; the role of medical professionals and community development workers in community health programmes; the role and training of community health workers; and financing community health programmes. The hypothesis of the investigation was that all three categories of programme participants in one programme share attitudes distinct from participants in the other two programmes. Although, due to technical reasons, it was not possible to test this hypothesis, the survey produced other conclusions.(ABSTRACT TRUNCATED AT 250 WORDS)

Health care in China: the experts take command.

PIP: The trend away from communal medicine in the People's Republic of China is sadly noted in this brief history of medical education and practice in Mainland China since the cultural revolution. After Mao's revolution, the institution of barefoot doctors and other paraprofessional personnel spread quickly; entrance to medical colleges required a commitment to the common good, not just intellectual qualifications. The end result was the education of a wide variety of people in the medical profession. However, recently, new policy states that intellectual or mental labor is equivalent to physical labor, and again medical college applicants are mainly children of intellectuals; the colleges are no longer drawing committed, although ill-educated, persons into their ranks. This author believes that the Chinese model taught others how they could get meximum health benefits from minimum financial investments through the use of preventive work, barefoot doctors, traditional medicines, and health campaigns. It also encouraged, especially in developing countries, breaks from traditional Western approaches to health care and encouraged developments of health care systems concerned more with the care of the masses of rural and urban poor rather than the praise of the medical profession housed in Western countries. The Chinese would do well to learn from their own past.^ieng