[EFFECTIVENESS OF PREVENTIVE VACCINE PROPHYLAXIS OF CHICKEN POX IN MILITARY COLLECTIVES].

AIM: Study the effectiveness of preventive vaccine prophylaxis of chicken pox in military collectives. MATERIALS AND METHODS: In the focus of chicken pox, 200 servicemen of the new addition by conscription were immunized once against chicken pox; 97 servicemen by conscription of the new addition (comparison group) were not vaccinated. Epidemiologic and immunologic effectiveness of conduction of preventive vaccine prophylaxis in chicken pox focus were studied. RESULTS: In the group of 200 soldiers, that were present in the focus of infection and were immunized once against chicken pox, only 2 cases of this disease were registered (10 per thousand). In the comparison group, that consisted of 97 unvaccinated servicemen, chicken pox disease was registered in 7 individuals (72 per thousand). Epidemiologic effectiveness of preventive vaccine prophylaxis of chicken pox amounted to 86%. Immunologic effectiveness of vaccination 2-3 weeks after the immunization was 42%, and 2 months after--44%. Local reactions in the form of hyperemia (up to 1.5 cm) and edema were noted in 10% of the vaccinated at the location of preparation administration; in 1.7%--general reaction in the form of temperature increase to 37.8°C was observed. Post-vaccinal complications in the immunized group were not detected. CONCLUSION: Preventive vaccination of servicemen allows to minimize the spread of chicken pox, however can not serve as means of complete elimination of the infection from military collectives.

[Epidemiological peculiarities of acute respiratory diseases and its prophylaxis in Internal Troops of the Ministry of Internal Affairs].

Acute respiratory diseases is the current problem of the medical service and command of Internal Troops of the Ministry of Internal Affairs. In recent years on the background of increase of contract servicemen the total rate of these infections decreased. However, rate of acute respiratory diseases in conscripts remains high and has a tendency to increase. Due to restricted possibilities of immunization in case of acute respiratory diseases it is necessary to improve the non-specific prophylaxis. The guaranty of sanitary-and-epidemiological safety may be active participation of military-and-professional society in realization of lifesaving measures and safety of military service.

[Some epidemiological aspects of respiratory diseases in in-country forces of the ministry of internal affairs of the Russian Federation in 1998-2011].

The authors analyzed the rate of respiratory diseases among the contingent of the in-country forces of the Ministry of Internal Affairs of the Russian Federation for the last 14 years. It was noted that the rate of respiratory diseases in 2009-2011 is higher than in 1998-2007. It is concerned with cold aggression and factors of intermigling of contingent, and also with mistakes of prevention actions. Improvement of life quality during all types of military service, improvement of prevention measures is necessary for the complex prevention of respiratory diseases.

[The analysis of biochemical markers of MCF-7 cells apoptosis induced by recombinant analog of lactaptin].

Earlier we isolated and characterized human milk pro-apoptotic peptide - lactaptin and generated its engineered analog - RL2. It was shown that both lactaptin and RL2 are capable to induce apoptotic death of MCF-7 cells. In this report we have analyzed biochemical markers of RL2 induced MCF-7 apoptosis. The activation of initiator and effector caspases as well as mitochondrial membrane potential and cytoplasm membrane changes were analyzed using flow cytometry and Western-blot methods. We have found that RL2 induced apoptotic death of MCF-7 cells was accompanied by PS exposure on the plasma membrane surface. It also was shown that RL2 has induced dissipation of mitochondrial membrane potential and resulted in activation of initiator caspases 8, 9 and effector caspase 7.

[Epidemiological and environmental specifications of acute tonsillitis (angines) in contract servicemen of internal troops of the Russian Interior Ministry].

The features of disease tonsillitis (angines) in contract servicemen of internal troops of the Russian Interior Ministry in the period from 2000 to 2009 were studied. The authors calculated trends for long-term dynamics of its troops as whole and regional commands. The incidence of quinsy in the period under review tended to moderate reduction in the rate of 3.2% per year. The most significant increase in incidence is observed in a test in 2001 when there was formation of a large compound located in the field. In 2009, the share of sore throats in the structure of general morbidity of military personnel on a contract by 4.7%, the highest rate recorded in their autumn-winter period, due to the influence of "cold" factor.

[Epidemiological characteristics of acute tonsillitis in military draft personnel from troops of the Ministry of Interior of Russia].

Studied the incidence of angina conscripts in the Russian Interior Ministry troops in the period from 2000 to 2009, given its epidemiological characteristics, calculated rates of changes for the troops in general and regional commands. The incidence of angina tended to moderate growth with an average rate of 4.5% per year. The greatest increase in the frequency of sore throats was registered in 2009 during the reform period of Interior troops, when their share in the overall incidence of conscripts reached 5.9%. The structure of the monthly incidence of maximum values were recorded twice a year--in January and July (the first few months of winter and summer periods of study).

[Nonspecific prophylaxis of tonsillitis in military drafted personnel of internal troops of Ministry of Internal Affairs of Russia].

The morbidity of tonsillitis in military drafting personnel of internal troops of Ministry of the Interior of Russia was high and had the up trend during 2000-2007. In 2008-2009 took place new increase of these diseases. The most unfortunate was the Second training center. Overcrowded accommodation of cadets in kasernes and complete rotation 2 times per year promoted this increase. The conditions of accommodation of cadets ware improved in 2010, preventive prophylaxis of tonsillitis was provided with the help of Bitsillin-5. As a result, the amount of ill in the Second training center during the summer 2010 was 5, 6 times lower than in the same period of 2009. It shows that the best antiepidemic effect can be achieved with combination of measures against the carry-over of causative agent and medicated prophylaxis.

[Community-acquired pneumonia among the conscripts of Interior Forces in Russia: characteristics of the epidemic process].

The dynamics of the incidence and characteristics of community-acquired pneumonia among the servicemen at the call of Interior Forces of Russia during the period 2000-2010 is analyzed. High frequency of pneumonia among the conscripts is provided by a number of factors: immunodepression during the period of adaptation to military service, the presence of a substantial portion of chronic respiratory disease, chill, overcrowded accommodation in the barracks and the "mixing factor". In recent years the level of this disease has had a positive effect of specific prophylaxis of pneumococcal infections and the transition period of one year of compulsory military service.

[Assessment of effectiveness and immunogenicity of hepatitis A vaccination in servicemen of Internal Forces of Ministry of Internal Affairs of Russia].

AIM: Comarative assessment of immunological and epidemiological effectiveness of hepatitis A vaccination and duration of immunity after immunization in servicemen. MATERIALS AND METHODS: During 1996-2003, immunogenicity and epidemiologic effectiveness of 3 vaccines against hepatitis A--Havrix 1440 (GlaxoSmithKline, Belgium), Hep-A-in-Vac (Chumakov Institute of Poliomyelitis and Viral Encephalitides, Russia), and Avaxim (Sanofi Pasteur, France)--were evaluated. More than 15,000 servicemen were immunized during that time. In all cases one-dose vaccination without booster was performed. RESULTS: Several cases of acute hepatitis A were registered 12 months and 6-8 months after vaccination in military communities immunized with Havrix 1440 and Hep-A-in-Vac vaccines respectively. Usage of Avaxim vaccine as a single dose in field trials allowed to exclude new cases of acute hepatitis A during time of military service. It was shown that 5 years after single vaccination with Avaxim protective anti-HAV antibody level (20 IU/l) persisted in 90% of Internal Forces servicemen. CONCLUSION: On the basis of performed complex of studies, system of antiepidemic measures was developed, which leads to decrease of hepatitis A incidence. Selective immunization is proposed.

[Neutralizing single-chain antibodies against the tick-borne encephalitis virus].

A recombinant pSC13D6 plasmid DNA was constructed based on cDNA fragments of genes encoding variable domains of heavy and light chains of the MKA 13D6 monoclonal antibody against glycoprotein of the tick-borne encephalitis (TBE) virus. This plasmid provided expression in Escherichia coli cells of the sc13D6 single-chain antibody against the TBE virus. The produced antibodies could bind to the TBE virus, strain 205, and the TBE virus recombinant E protein. The affinity constant of purified sc13D6 was (3.0 +/- 0.2) x 10(7) M(-1) for the equilibrium state and (2.8 +/- 0.3) x 10(7) M(-1) in the case of antigen-antibody formation on the surface. The obtained single-chain antibody could inhibit the infection potency of the TBE virus on a monolayer of eukaryotic cells. The calculated IC50 value for sc13D6 was 16.7 microg/ml.

[Evaluation of influenza vaccine Vaxigrip in the combined immunization of conscripts during influenza seasons of 2002-2003]. / Opyt primeneniia grippoznoi vaktsiny Vaksigrip pri sochetannoi immunizatsii prizyvnikov srochnoi sluzhby v épidemicheskom sezone 2002-2003 gg.

The immunization properties of the influenza vaccine Vaxigrip, used in combination with vaccines against pneunococcal infection and hepatitis A (respectively, Pneumo 23 and Avaxim), were evaluated. In Central Russia in one of the units of the internal forces of the RF Ministry of Internal Affairs 3 groups totaling 755 servicemen were formed, depending on the complex of the introduced vaccines. Active medical observation and the registration of the complaints of the vaccinees at the postvaccinal period did not reveal unusual reactions and complications in none of the groups under observation. In the evaluation of the level of specific antibodies to the circulating influenza viruses prior to vaccination the low level of collective protection to influenza B virus was determined: protective antibody levels were registered only in 14-18% of the servicemen, while the corresponding data with respect of influenza viruses A(H1N1) and A(H3N2) were 45-50% and 56-63% respectively. At the same time, in seronegative persons the vaccine Vaxigrip exhibited high immunogenic activity with respect of all 3 influenza strains; seroconversion to them was determined in 84-92% of the vaccines, and the level of protective antibody titers before the beginning of the epidemic season was 86-99% in the whole of the group. The characteristics of the prophylactic effectiveness of the vaccine Vaxigrip in relation to the influenza virus infection level 1 were 4.7 (index) and 79% (coefficient). In addition, the frequency of influenza cases, clinically pronounced and confirmed by laboratory methods, in patients who had been immunized with 3 vaccines was 6.7%, which was 10.3 times less frequent than number of cases in the groups of comparison (68.2% on the average). The coefficient of epidemiological effectiveness of the prophylaxis of influenza was 90.2%. The complex use of 3 preparations did not affect the immunization properties of the vaccine Vaxigrip.

[Interaction of oligonucleotides and ATP with preparations of sIgA possessing protein kinase activity]. / Vzaimodeistvie oligonukleotidov i ATP s preparatami sIgA, obladaiushchimi proteinkinaznoi aktivnost'iu.

Interaction of secretory immunoglobulins A of a varying degree of purity with oligonucleotides and ATP has been studied by the method of affinity modification. For this aim we used reactive derivatives of 32P-labeled deoxyoligonucleotide (ClRCH2NHp(T)14) and [gamma-32P]ATP (ClR-32PppA) or ATP (ClR-pppA) bearing a 4-[(N-2-chloroethyl-N-methyl)amino]benzylamine residue. Preparations of sIgA were obtained from human milk by sequential chromatography on protein A-sepharose (P1), DEAE-fractogel (P2) and by gel-filtration in 50 mM NaOH (P3). It was revealed, that the H- and L-chains of sIgA P1; H-, L-chains and secretory component (SC) in sIgA P2 and only SC in sIgA P3 were exposed to modification after incubation with ClRCH2NHp(T)14. LPS, DNA, tRNA, heparin, sufficiently inhibited the modification of chains of sIgA P1. These competitors did not influence the modification of H- and L-chains of sIgA P2, but DNA, tRNA, heparin, inhibited binding of SC with the modifier. Suppressing affect of binding of ClRCH2NHp(T)14 with secretory component of sIgA P3 by d(T)14 has been observed as well. The research of ClR-32PppA interaction with sIgA P3 has shown that H- and L-chains of sIgA are exposed to modification. ATP inhibited the reaction. Study of the influence of modification on the protein kinase activity of sIgA P3 has revealed, that the preliminary incubation of sIgA P3 with ClR-pppA leads to inhibition of protein kinase activity. We suggest that sIgA, possessing the protein kinase activity (sIgA-abzymes) has an ATP-binding center (catalytic center) and has an oligonucleotide-binding center as well.