RESUMO
Lung injury in preterm neonates with respiratory failure has been attributed to persistent inflammation, which is likely to involve lung macrophages (LM). The study objective was to investigate LM during the first 8 d of life from preterm infants (n = 19), using term infants (n = 11) with respiratory failure as control subjects. LM percentages from mixed-cell suspensions produced from tracheobronchial lavage were calculated. A postnatal increase in the mean LM concentration was demonstrated within the preterm group (p = 0.01), which was greater in comparison to that from the term group (p < 0.01). Regression analyses were significant for direct relationships between LM concentrations and ex vivo lipopolysaccharide-induced tumor necrosis factor-alpha and IL-10 production (r = 0.93 and r = 0.63, respectively), establishing LM as the source of these cytokines. Comparative analyses demonstrated that the ability of preterm versus term LM to produce tumor necrosis factor-alpha was nearly identical; in contrast, a trend toward diminished levels of IL-10 expression in the preterm group was observed (p = 0.06). Thus, although studies have shown that LM precursors (i.e. cord blood monocytes) produce less tumor necrosis factor-alpha in preterm versus term infants, the present data strongly suggest that this relationship does not hold postnatally with respect to terminally differentiated LM in sick neonates. Overall, the data are consistent with a pro- versus antiinflammatory imbalance that may bear functional significance on the pathogenesis of chronic lung disease.
Assuntos
Recém-Nascido/fisiologia , Recém-Nascido Prematuro/fisiologia , Interleucina-10/biossíntese , Lipopolissacarídeos/farmacologia , Macrófagos Alveolares/fisiologia , Fator de Necrose Tumoral alfa/biossíntese , Humanos , Doenças do Recém-Nascido/fisiopatologia , Macrófagos Alveolares/citologia , Macrófagos Alveolares/efeitos dos fármacos , Análise de RegressãoRESUMO
No other disease entity has provided greater impetus for the development of the concept of "molecular morphology" than Hodgkin lymphoma. Efforts to understand the etiology of this enigmatic disease have stimulated the application and refinement of almost every mode of biomedical scientific exploration. Notwithstanding the vast amount of data generated, much is still unknown about this remarkable disease, serving as an ongoing inducement to the development and application of new technologies for the analysis of cells and molecules in a morphologic environment.
