RESUMO
Adeno-associated virus type-2 (AAV-2) is a helper-dependent parvovirus that has been implicated in the inhibition of replication and oncogenic transformation of bovine papillomavirus type-1 (BPV-1) and other transforming DNA viruses. Previous studies have suggested that the Rep78 protein of AAV-2 is a key player mediating this effect. In this report we have analyzed the effect of AAV-2 Rep78 protein on the regulation of gene expression of a reporter gene under the control of the long control region (LCR) of BPV-1. Our results show that Rep78 is capable of down-regulating the promoter activity of the LCR in vivo in tissue culture cells. Inhibition of LCR activity in vivo suggested the need for Rep78 to bind to a region of the LCR promoter spanning the E2-responsive elements of BPV-1. This observation was further confirmed in vitro with gel shift assays showing specific binding of Rep78 to DNA oligonucleotides containing E2-responsive element 1 (E2RE1) sequences of BPV-1 LCR. Our results expand the understanding of the mechanism of trans-regulation mediated by Rep78 and involving this protein and DNA sequences with complex secondary structure.
Assuntos
Papillomavirus Bovino 1/genética , Papillomavirus Bovino 1/metabolismo , Proteínas de Ligação a DNA/genética , Proteínas de Ligação a DNA/metabolismo , Dependovirus/metabolismo , Elementos de Resposta , Proteínas Virais/genética , Proteínas Virais/metabolismo , Animais , Sequência de Bases , Bovinos , Linhagem Celular Transformada , Proteínas de Ligação a DNA/biossíntese , Proteínas de Ligação a DNA/isolamento & purificação , Dependovirus/genética , Regulação para Baixo/genética , Escherichia coli/genética , Escherichia coli/metabolismo , Regulação Viral da Expressão Gênica , Humanos , Dados de Sequência Molecular , Regiões Promotoras Genéticas , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/genética , Especificidade por Substrato , Sequências Repetidas Terminais , Proteínas Virais/biossíntese , Proteínas Virais/isolamento & purificaçãoRESUMO
A study was undertaken on wild animals to determine the seroprevalence of animal leptospirosis in Korea. Using the serum microagglutination test for 19 serogroups, it was shown that two of 26 rats (Rattus rattus) had antibodies to Leptospira canicola. When data for domestic animals were included, the most prevalent (nearly 50%) serogroup was Leptospira canicola.
Assuntos
Doenças das Aves/epidemiologia , Leptospira/imunologia , Leptospirose/veterinária , Mamíferos , Testes de Aglutinação , Animais , Animais Selvagens , Anticorpos Antibacterianos/sangue , Aves , Surtos de Doenças/prevenção & controle , Coreia (Geográfico)/epidemiologia , Leptospirose/epidemiologia , Muridae , Prevalência , Ratos , Doenças dos Roedores/epidemiologia , ZoonosesRESUMO
With regard to the legal regulations for the diagnosis of Aujeszky's disease in pigs, a retrospective immunoenzymatic study was performed on brains of 20 pigs, in which Aujeszky's disease had been diagnosed based only on clinical, macroscopical and histological findings, although the etiological agent could not be demonstrated neither by immunofluorescent technique nor by virological cultivation. Applying the peroxidase-antiperoxidase-(PAP) method, in 6 of 20 animals (30%) viral antigen of porcine herpesvirus type 1 was demonstrated in situ in at least two of five different locations of the brain. Reasons for negative results in immunohistological and virological investigations are discussed and the significance of the performed immunoenzymatic technique is pointed out.
Assuntos
Antígenos Virais/análise , Encéfalo/microbiologia , Herpesvirus Suídeo 1/imunologia , Pseudorraiva/diagnóstico , Doenças dos Suínos/diagnóstico , Animais , Feminino , Técnicas Imunoenzimáticas/veterinária , Imuno-Histoquímica , Masculino , SuínosAssuntos
Adenoma/veterinária , Colelitíase/veterinária , Neoplasias da Vesícula Biliar/veterinária , Doenças dos Macacos/complicações , Adenoma/complicações , Adenoma/patologia , Animais , Colelitíase/complicações , Colelitíase/patologia , Neoplasias da Vesícula Biliar/complicações , Neoplasias da Vesícula Biliar/patologia , Haplorrinos , Masculino , Doenças dos Macacos/patologia , PapioRESUMO
An investigation was conducted to delineate the fine structure of steroid-producing ovarian theca interna cells following administration of Korean Panax ginseng to rats for 60 days. The cytoplasmic changes were observed in the ginseng-treated theca interna cells, increased number, size and density of the mitochondria, and increased size of the smooth surfaced endoplasmic reticulum, the rough surfaced endoplasmic reticulum and the Golgi apparatus. The nucleus and nucleolus were slightly enlarged and increased numbers of dense bodies were seen whereas lipid droplets were decreased in number. The changes may result from hyperfunction of the steroid-producing cells. Morphologic changes seen may represent stimulating effects on the steroid-producing cells of the theca interna in ginseng-treated animals.