RESUMO
Objective: To investigate the chemical constituents of Clematis aethusifolia,a traditional Mongolian medicine for resolving hard lump. Methods: Bioactive guided isolation and purification of Clematis aethusifolia was performed by normal and reverse phase column chromatography, Sephadex LH-20 and preparation HPLC. The structures were identified by 1D,2D-NMR and MS spectral analysis and comparison with literature data. Cytotoxicity of compounds were determined by CCK-8 cell staining method for detecting growth inhibition to five kinds of human solid tumor cell lines. Results: Eight compounds were isolated and identified as vanillicacid( 1),protocatechuic acid( 2),( +)-pinoresinol diglucoside( 3),( +)-pinoresinol-4'-O-ß-D-glucopyranoside( 4),( +)-syringaresinol-4'-O-ß-Dglucopyranoside( 5),7,9,9'-trihydroxy-3,3'-dimethoxy-8-O-4'-neolignan-4-O-ß-D-glucopyranoside( 6),butyl-ß-D-fructopyranoside( 7),butyl-ß-D-fructofuranoside( 8). Compounds 2,8 showed strong cytotoxic activity. Conclusion: All the compounds are isolated from this plant for the first time, Results: Eight compounds were isolated and identified as vanillicacid( 1), protocatechuic acid( 2),( +)-pinoresinol diglucoside( 3),( +)-pinoresinol-4'-O-ß-D-glucopyranoside( 4),( +)-syringaresinol-4'-O-ß-Dglucopyranoside( 5),7,9,9'-trihydroxy-3,3'-dimethoxy-8-O-4'-neolignan-4-O-ß-D-glucopyranoside( 6),butyl-ß-D-fructopyranoside( 7),butyl-ß-D-fructofuranoside( 8). Compounds 2,8 showed strong cytotoxic activity. compounds 3,6 ~ 8 are isolated from Clematis genus for the first time, compounds 6 and 8 are isolated from Ranunculaceae family for the first time. Compounds 2,8 may be the effective components in Clematis aethusifolia.
