1.
Org Biomol Chem
; 15(21): 4536-4539, 2017 May 31.
Artigo
em Inglês
| MEDLINE
| ID: mdl-28517015
RESUMO
Due to the lowered pKa of 4-fluorohistidine relative to histidine, peptides and proteins containing this amino acid are potentially endowed with novel properties. We report here the optimized synthesis of 4-fluorohistidine and show that it can efficiently replace histidine in in vitro translation reactions. Moreover, peptides containing 6×-fluorohistidine tags are able to be selectively captured and eluted from nickel resin in the presence of his-tagged protein mixtures.